Browse > Article
http://dx.doi.org/10.7783/KJMCS.2013.21.2.142

Induction and Growth of Vegetative Stems through In Vitro Culture of Gastrodia elata  

Kim, Hyun Tae (Department of Forest Genetic Resources, Korea Forest Research Institute)
Kim, Seung Taek (Department of Forest Genetic Resources, Korea Forest Research Institute)
Lee, Wi Young (Department of Forest Genetic Resources, Korea Forest Research Institute)
Park, Eung Jun (Department of Forest Genetic Resources, Korea Forest Research Institute)
Publication Information
Korean Journal of Medicinal Crop Science / v.21, no.2, 2013 , pp. 142-147 More about this Journal
Abstract
Gastrodia elata has been cultivated as an important medicinal resources to treat various human diseases. One of the major problems associated with its field production is the degeneration of seed tubers, which is mainly caused by soil-borne pathogens. This study was conducted to produce disease-free seed tubers by the development of in vitro micropropagation method. First, tubers of G. elata were treated with $HgCl_2$ prior to culturing in vitro. Among various culture medium tested, water agar (WA) and WPM medium were the most effective on the induction and growth of vegetative stems. NAA ($0.1mg/{\ell}$) or TDZ ($1.0mg/{\ell}$) in WA medium showed better growth of vegetative stems compared to other plant hormones. Finally the induction and growth of vegetative stems were better in the dark compared to the light condition. In this study, we established an in vitro micropropagation system of G. elata, which might be an efficient way to increase the yield and quality of G. elata tubers in the field production.
Keywords
Gastrodia elata Blume; Immature Rhizome; Micro-Propagation; Vegetative Stem; Orchidaceae;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Ahn YK, Na SY, Kim SY and Om YH. (2002). The effects of photoperiods on In vitro microtuberization in potato(Solanum tuberosum L.). Horticulture Environment and Biotechnology. 301-305.   과학기술학회마을
2 Bae KH, Ko MS, Choi SA, Lee HB, Kim NY, Song JM and Song G. (2012). In vitro germination of Gastrodia verrucosa Blume and Hetaeria sikokiana Tuyama treated by NaOCl. Journal of Plant Biotechnology. 39:163-168.   과학기술학회마을   DOI   ScienceOn
3 Bin H and Chen F. (2004). Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter current chromatography. Journal of Chromatography A. 1052:229-232.   DOI   ScienceOn
4 Chang HM and But PH. (1986). Pharmacology and application of chinese materia medica(Vol. 1). World Scientific Publishing Co. Singapore. p.185.
5 Choi SO, Chung JD and Lee JH. (1996). Effect of culture media on rhizome formation and its subsequent growth from shoot tip culture of temperate Cymbidium species. Journal of Plant Biotechnology. 23:167-172.
6 Choi SR, Kim CS, You DH, Kim JY, Kim YH, Ahn YS, Kim JM, Kim YS and Seo KW. (2011). Changes of components and quality in gastrodiae rhizoma by different dry methods. Korean Journal of Medicinal Crop Science. 19:354-361.   과학기술학회마을   DOI   ScienceOn
7 Chung JD and Chun CK. (1983). Effect of basal media and growth regulators on germinination of seeds and shoot emergence from rhizomes. Horticulture Environment and Biotechnology. 24:236-242.
8 Chung JD, Harm SH, Jee SO, Chung MY, Khin TM and Kim CK. (2004). The multiple propagation of mericlones from shoot tip culture of Neofinetia Falcata. Flower Research Journal. 12:101-106.
9 Kang KW, Park KS, Mo SY, Kim DH and Kang SY. (2009). A new Cymbidium orchid variety "Daegook" bred by in vitro mutagenesis. Korean Journal of Breeding Science. 41:210-514.   과학기술학회마을
10 Kang TJ and Yang DC. (2003). Days to germination and effect of growth regulator on rhizome growth in Cymbidium goeringii hybrid. Korean Journal of Plant Resources. 6:144-148.   과학기술학회마을
11 Kim YG, Kim MG, Yoon S and Hong JS. (2000). Histological observation on the symbiotic relationships between Gastrodia elata and rhizomorph of Armillaria mellea. The Korea Journal of Mycrobiology. 28:41-45.   과학기술학회마을
12 Kim MS, Lee YR, Won JY, Kim JY, Kim BH and Eun JS. (1998). Effects of days after pollination and media asymbiotic germination in cross combination of Cymbidium spp. Horticulture Environment and Biotechnology. 19:236-238.
13 Kim HT, Kim JA and Park EJ. (2012). Genetic diversity and metabolite analysis of Gastrodia elata by Inter-Simple Sequence Repeats(ISSR) Markers. Korean Journal of Medicinal Crop Science. 20:440-446.   과학기술학회마을   DOI   ScienceOn
14 Kim JY and Lee JS. (1992). Effect of cultural conditions on rhizome growth and organogenesis of Cymbidium lancifolium native to Korea in vitro. Horticulture Environment and Biotechnology. 33:471-476.
15 Lee BY, Choi HS and Hwang JB. (2002). Analysis of food components of Gastrodiae Rhizoma and changes in several characteristics at the various drying conditions. Korean Society of Food Science and Technology. 34:37-42.   과학기술학회마을
16 Lee MY. (2007). Important medicinal crop characteristics and cultivation technologies. Dongguk University Press. Korea. p.39.
17 Sung KC, Mi YC, Kim CK, Lim KB and Chung JD. (2006). Effect of culture media on rhizome formation and subsequent growth from shoot tip culture of Cymbidium goeringii. Flower Research Journal. 14:289-295.
18 Xu Q, Liu Y, Wang X, Gu H and Chen Z. (1998). Purification and characterization of a novel anti-fungal protein from Gastrodia elata. Plant Physiology of Biochemistry. 36:899-905.   DOI   ScienceOn
19 Hayashi J, Sekine T, Deguchi S, Lin Q, Horie S, Tsuchiya S, Yano S, Watanabe K and Ikegami F. (2002). Phenolic compounds from Gastrodia rhizome and relaxant effects of related compounds on isolated smooth muscle preparation. Phytochemistry. 59:513-519.   DOI   ScienceOn
20 Ha JH, Lee DU, Lee JT, Kim JS, Yong CS, Kim JA, Ha JS and Huh K. (2000). 4-hydroxybenzaldehyde from Gastrodia elata B1. is active in the antioxidation and gabaergic neuromodulation of the rat brain. Journal of Ethnopharmacology 73:329-333.   DOI   ScienceOn
21 Heo JC, Park JY, An SM, Lee JM, Yun CY, Shin HM, Kwon TK and Lee SH. (2006). Anti-oxidant and anti-tumor activities of crude extracts by Gastrodia elata Blume. Korean Food Marketing Association. 13:83-87.   과학기술학회마을
22 Huang ZL. (1985). The pharmacological studies and clinical application of Gastrodia eleta Bl. Journal of Modern Development Traditional Media. 5: 251-254.