• Biomolecules & Therapeutics
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• 제26권3호
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• pp.282-289
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• 2018

Melanin is a pigment produced from tyrosine in melanocytes. Although melanin has a protective role against UVB radiation-induced damage, it is also associated with the development of melanoma and darker skin tone. Tyrosinase is a key enzyme in melanin synthesis, which regulates the rate-limiting step during conversion of tyrosine into DOPA and dopaquinone. To develop effective RNA interference therapeutics, we designed a melanin siRNA pool by applying multiple prediction programs to reduce human tyrosinase levels. First, 272 siRNAs passed the target accessibility evaluation using the RNAxs program. Then we selected 34 siRNA sequences with ${\Delta}G{\geq}-34.6kcal/mol$, i-Score value ${\geq}65$, and siRNA scales score ${\leq}30$. siRNAs were designed as 19-bp RNA duplexes with an asymmetric 3' overhang at the 3' end of the antisense strand. We tested if these siRNAs effectively reduced tyrosinase gene expression using qRT-PCR and found that 17 siRNA sequences were more effective than commercially available siRNA. Three siRNAs further tested showed an effective visual color change in MNT-1 human cells without cytotoxic effects, indicating these sequences are anti-melanogenic. Our study revealed that human tyrosinase siRNAs could be efficiently designed using multiple prediction algorithms.

• 전기전자학회논문지
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• 제6권1호
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• pp.72-79
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• 2002

본 논문에서는 복잡한 배경의 영상에서 얼굴을 검출하는 효과적인 알고리즘을 제안한다. 제안된 알고리즘은 색상정보와 PCA-LDA(Principal Component Analysis - Linear Discriminant Analysis)에 의하여 구해진 고유얼굴 (eigenface)을 이용한다. 색상정보는 다른 어떤 방법보다 얼굴을 검출하는데 유용하게 사용된다. 고유얼굴은 전체학습 얼굴들의 평균정보를 포함하고 있기 때문에 얼굴 후보영역에 대하여 얼굴인지를 판별할 수 있는 기능을 갖는다. 전체적인 과정은 두 단계로 이루어진다. 첫 번째 단계는 영상내에서 색상정보를 이용하여 살색영역의 1차 얼굴 후보영역을 찾아 후보영역의 위치와 기를 결정한다. 두 번째 단계는 1차 얼굴 후보영역에 대하여 PCA-LDA에 의한 얼굴의 유사성을 측정하여 얼굴인지 아닌지를 판별한다. 제안한 검출 방법을 사용한 실험 결과, 색상정보를 사용하여 1차 얼굴 후보영역의 크기와 위치를 결정함으로써 검출속도의 향상을 가져올 수 있었다. 또한 PCA-LDA에 의한 고유얼굴과의 비교를 통해 복잡한 매경이 있는 영상에서도 97%의 검출률을 얻을 수 있었다.

• 디지털콘텐츠학회 논문지
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• 제19권9호
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• pp.1653-1662
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• 2018

본 연구는 화장품 앱이 제공하는 가상 메이크업 템플릿과 메이크업 룩의 관계를 조사하였다. 한국의 20대 및 30대 여성 34명을 대상으로 설문조사한 결과, 19개 가상 메이크업 템플릿중 'Bella', 'Hilary', 'Cara' 등 3개를 가장 선호하며, 이에 상응하는 메이크업 룩은 각각 '섹시', '클래식', '내추럴'인 것으로 조사되었다. 전문가의 정성적 평가와 통계적 분석에 의한 정량적 평가를 통해 이들은 메이크업 템플릿과 유사한 메이크업 룩을 선호하고, 전반적으로 고명도의 연한 피부색과 중명도의 밝은 입술색을 선호하는 것으로 분석되었다.

• 대한한방내과학회지
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• 제31권1호
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• pp.118-127
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• 2010

Objective : Hyperhidrosis is the secretion of sweat in amounts greater than physiologically needed for thermoregulation. Ryodoraku is a physiological function test using electric current, and is closely related to skin sympathetic tone. The aim of this study was to investigate the characteristics of Ryodoraku and association of Ryodoraku with hyperhidrosis. Methods : For this study, we conducted Ryodoraku on 56 people, comprising 33 with palmar-plantar hyperhidrosis and 23 with head-face hyperhidrosis, who visited the 5th Internal Department of the Oriental Medicine Hospital of Kyung Hee University during the period from March 1, 2008 to May 31, 2009. Ryodoraku scores were evaluated and compared by 7 indices(upper, lower, left, right, yin, yang, total score). Results : Ryodoraku scores were significantly higher in hyperhidrosis patients than the normal group. Ryodoraku scores of palmar-plantar hyperhidrosis patients were significantly higher than of head-face hyperhidrosis patients and the normal group. The difference of Ryodoraku indices among palmar-plantar hyperhidrosis patients were insignificant. In head-face hyperhidrosis patients, the upper index was significant higher than the lower index. Conclusions : In hyperhidrosis patients, all indices were significant higher than lower. The upper/lower ratio was significant higher in head-face hyperhidrosis patients.

• 대한한방내과학회지
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• 제29권3호
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• pp.535-542
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• 2008

Objectives : Ryodoraku, which is a physiological function test using electric current, is closely related to skin sympathetic tone. Pulse analysis is known to reflect cardiovascular reactivity. Refer to the previous researches Ryodoraku and pulse analysis have value as tools for diagnosing respiratory diseases. In this study we examined the diagnostic values of Ryodoraku and pulse analysis for respiratory disease patients. Methods : For this study. we conducted Ryodoraku and pulse analysis on 114 people, including 83 respiratory disease outpatients and 31 volunteers who did not have any respiratory symptoms or disease history. The respiratory patients were divided into three subgroups according to their symptoms: rhinorrhea group, cough-sputum group and wheezing-dyspnea group. Then we compared the disease groups with the control group. Results : When all experimental groups were compared with the control group, mean Ryodoraku was significantly lower. Mean H2, mean H3 and mean H6 were significantly lower in the rhinorrhea group (P<0.05), all the test results of Ryodoraku were evidently lower in the cough-sputum group (P<0.01), and most results of Ryodoraku were evidently lower in the wheezing-dyspnea group except H1 (P<0.01). Compared with the control group on pulse analysis, mean YP+/YP- was significantly lower in the wheezing-dyspnea group (P<0.05). Conclusion : Ryodoraku and pulse analysis were found to have a high value as quantitative diagnosis tools reflecting individuals' weakness and firmness. Nevertheless, more research is needed to find the further values.

• 한국감성과학회:학술대회논문집
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• 한국감성과학회 1998년도 추계학술발표 논문집
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• pp.225-229
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• 1998

The purpose of the current study was comparative analysis of autonomic and electrocortical responses to passive and active touch of the tektites with different subjective emotional preference. Perspective goal of the project is development of a template for classification of tactile stimuli according to subjective comfort and associated physiological manifestations. The study was carried out on 36 female college students. Physiological signals were acquired by Grass and B10PAC 100 systems with AcqKnowledge III software. Frontal, parietal and occipital EEG (relative power spectrum /percents/ of EEG bands - delta, theta, slow and fast alpha, low and fast beta), and autonomic variables, namely heart rate (HR), respiratory sinus arrhythmia (RSA), pulse transit time (PTT), respiration rate (RSP) and skin conductance parameters (SCL, amplitude, rise time and number of SCRs) were analyzed for rest baseline and stimulation conditions. Analysis of the overall pattern of reaction indicated that autonomic response to tactile stimulation was manifested in a form of moderate HR acceleration, RSP increase, RSA decrease (lowered vagal tone), decreased n and increased electrodermal activity (increased SCL, several SCRs) that reflects general sympathetic activation. Parietal EEG effects (on contra-lateral side to stimulated hand) were featured by short-term alpha-blocking, slightly reduced theta and significantly increased delta and enhanced fast beta activity with few variations across stimuli. The main finding of the study was that most and least preferred textures exhibited significant differences in autonomic (HR, RSP, PTT, SCR, and at less extent in RSA and SCL) and electrocortical responses (delta, slow and fast alpha, fast beta relative power). These differences were recorded both in passive and active stimulation modes, thus demonstrating reproducibility of distinction between most and least emotionally preferred tactile stimuli, suggesting influence of psychological factors, such as emotional property of stimulus, on physiological outcome. Nevertheless, development of sufficiently sensitive .and reliable template for classification of emotional responses to tactile stimulation based on physiological response pattern may require more extensive empirical database.

• 대한한방내과학회지
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• 제28권3호
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• pp.560-569
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• 2007

Objectives : Ryodoraku is a physiological function test using electric current, and is closely related to skin sympathetic tone. Pulse analysis is known to reflect cardiovascular reactivity. There has been no report on the correlation between ryodoraku and pulse analysis in respiratory diseases. The present study examined the diagnostic values of ryodoraku and pulse analysis for respiratory disease patients Methods : For this study, we conducted ryodoraku and pulse analysis in 103 people including 79 respiratory disease outpatients who visited the $5^{th}$ Internal Department of the Oriental Medicine Hospital of Kyung Hee University during the period from January 1, 2003 to July 25, 2006, and 24 volunteers who did not have any respiratory symptoms or disease history. The respiratory disease patients were divided into five sub-groups according to their symptom: cough-sputum group, wheezing-dyspnea group ' nasal symptoms group. cold-prone group, and fatigue prone group. We compared the disease groups with the control group in six items as follows : mean ryodoraku, mean H1, mean elastic index, the percentage of those with mean ryodoraku below $40{\mu}A$, the percentage of those with HI beyond the physiological range, and the ratio of left : right of elastic index Results and Conclusions : Ryodoraku and pulse analysis were found to have a high value as quantitative diagnosis tools reflecting individuals' weakness and firmness. The results of this research suggest that ryodoraku and pulse analysis have value as tools for diagnosing respiratory diseases.

• 감성과학
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• 제1권2호
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• pp.13-22
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• 1998

The sense of touch has both objective and subjective characteristics. During hand evaluation of the fabrics. psycho physiological processes such as emotion and stimulation. On other site, the mode of touch (passive vs. active) is also capable to modulate somatosensory responses. I.e., suppress somatocensory perception during active electrocortical responses to passive and active touch of the textiles with different subjective emotional preference. The study was carried out on 36 female college students. Physiological signals were acquired by Grass and BIOPAC 100 systems with AcqKnowledge variables, namely heart rate (HR), respiratory sinus arrhythmia (RSA), pulse transit time (PTT), respiration rate (RSP) and skin conductance parameters (SCL, amplitude, risetime and number of SCRs) were analyzed for baseline and stimulation conditions. Analysis was manifested in a form of moderate HR acceleration. RSP increase, RSA decrease (lowered vagal tone), decreased PTT and increased electrodermal activity (increased SCL, several SCRs) that reflects general sympathetic activation. Parietal EEG effects (on contra-lateral side to stimulated hand)were featured by short-term alpha-blocking, slightly reduced theta, significantly increased delta and enhanced fast beta activity with few variations across stimuli. The main finding of the study was that most and least preferred textures exhibited significant differences in autonomic (HR, RSP, PTT, SCR, and at less extent in RSA and SCL) and electrocortical responses (delta, slow and fast alpha, fast beta relative power). These differences were recorded both in passive and active stimulation modes, thus demonstrating reproducibility of distinction between most and least emotionally preferred tactile stimuli, suggesting influence of psychological factors, such as emotional property of stimulus, on physiological outcome.

• 감성과학
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• 제12권1호
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• pp.33-44
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• 2009

본 연구는 방송 영상물을 시청할 때 나타나는 감성적으로 긍정적 내용과 부정적 내용의 차별적 영향에 대해 탐구하는 한편, 형식적 특성 중 하나인 편집속도와의 상호작용을 고찰하였다. 연구는 실험으로 진행하였으며, 피험자가 메시지를 보는 동안 심박률과 피부 전도성 측정을 통해 주의와 각성 수준을 관찰하였다. 전체 메시지를 다 본 후 피험자들은 자유회상기억에 관한 문항을 작성하였다. 연구의 결과는 각성 수준을 통제하였을 때, 수용자들은 긍정적 내용보다 부정적 내용에 더 주의를 기울였지만 기억하는 정도는 반대로 긍정적 내용이 우세하였다. 한편 편집속도를 높이는 것이 감성적인 내용에 대한 반응에서 각성, 주의, 그리고 기억 수준을 높이는 것으로 나타났다. 하지만 그 효과는 긍정적, 부정적 내용에 따라 차별적이었다. 편집속도를 높이는 것이 부정적인 내용에는 별로 효력이 없거나 오히려 악영향을 미칠 수 있다는 결과가 도출되었다. 본 연구는 감성 연구에서 감성 유인가의 비대칭성에 대한 이해를 심화하고, 또한 감성 유인가적 내용과 형식적인 특성과의 상호작용이라는 새로운 연구 영역을 제시한다.

• 대한화장품학회지
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• 제25권1호
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• pp.37-54
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• 1999

분체 특성으로 매끄러운 사용감과 피부 부착력이 우수하며 판상의 체질안료보다 유동성이 큰 실리카 미립자를 제조하기 위해 실리카 조대입자의 제조와 이의 다단계 분쇄 공정을 이용하여 여러 층의 terrace를 가지고 있는 판상 체질안료의 표면과 다른 평골한 표면 상태의 평탄면을 가지는 실리카 미립자를 제조하였다 여기에서 제조된 실리카 단일 입자를 모입자로 사용함으로써 이산화티탄의 균일침전반응법에 의해 제조된 복합입자는 평탄면을 가지게 되어 유동성의 조절과 부착력의 조절에 의해 도포 층이 균일하게 형성되는 분체 특성을 가지고 있다 본 실험에서 가장 적합한 침전 반응의 조건은 요소농도가 0.2~0.3mol/$\ell$이고 황산농도가 0.3~O.4mol/$\ell$이고 황산티타닐의 농도가 0.007~0.015mol/$\ell$며, 반응온도는 60~8$0^{\circ}C$였다 이와 같은 균일 침전 반응의 조건에 따라 초미립자상 이산화티탄의 피복량과 피복된 초미립자의 입경을 제어함으로써 분체의 광학적 특성을 조절하였고, 그 결과 피부 도포 시 피부색에서 600nm이상의 장파장 영역에서 반사율을 증가시켜 강한 피부색 tone을 표현할 수 있도록 하였다.