• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
• /
• pp.387-412
• /
• 2003

Influence of gelatinase on basement membrane (BM) structure was investigated by using a skin equivalent (SE) model. The results showed that (1) gelatinase produced by cells degraded the BM and (2) the addition of matrix metalloproteinase-specific inhibitor to the SE medium accelerated the formation of BM structure, indicating that gelatinase is involved in BM impairment. The activity of gelatinase was also studied in healthy human facial skin tissues. The result of in situ zymography revealed gelatinase activity around the basal layer of the epidermis, where BM integrity was severely compromised. Therefore, this enzyme was suggested to be associated with BM decomposition in human facial skin. To assess the behavior of gelatinase in stratum corneum (SC) non-invasively, an immunological study was performed. Since positive immunostaining of pro-gelatinase B was observed in SC stripped from sun-exposed skin, whereas no positive staining detected in SC of non-irradiated skin, gelatinase in the epidermis could be non-invasively detected by measuring gelatinase in SC. Gelatinase in SC of healthy female volunteers was monitored using a special film that sensitively and conveniently detects gelatinase. Ninetr percent of SC from facial skin (l00 women, 40's-50's) was gelatinase-positive. On the other hand, SC from non-irradiated skin was negative. These results strongly suggest that (1) gelatinase is constantly produced in the facial epidermis of most middle-aged woman during their daily life, and (2) the enzyme might be involved in the aging-related degeneration of both BM and the matrix fibers of the upper layer of the dermis, acting as a very important aging factor. Strong inhibitory activity against gelatinase was found in turmeric extract and identified curcumin as the major ingredient. Topical application of cream containing turmeric extract significantly decreased the number of gelatinase-positive SC clusters in human facial skins. These results indicated that turmeric is an effective ingredient to prevent skin from photo aging by suppressing chlonically upregulated gelatinase activity by UV and to improve skin condition.

• 융합정보논문지
• /
• 제11권2호
• /
• pp.188-193
• /
• 2021

본 연구는 만 25세 이하의 여대생을 대상으로 스킨 도포 횟수에 따른 안면 피부의 수분함량 변화에 대한 연구를 하였다. 수분함량(moisture contents)은 피부 수분 측정기(피부 유수분측정기, PRC, 중국)를 이용하여 측정하였다. 스킨은 I사의 블루베리 리밸런싱 스킨[대용량] 500ml를 임상실험재료로 사용하였다. 이마와 눈가, 볼, 턱 모두 도포 전 보다는 한 겹 도포 후가 수분함량이 더 높았으며, 한 겹 도포 후 보다는 열 겹 도포 후 수분함량이 더 높은 것을 알 수 있다. 이와 같은 결과로 세안 후 기초 제품을 도포할 때 가장 첫 번째 단계인 스킨의 횟수를 늘려 도포 한 후 로션과 수분크림 등 기초 제품을 바른다면 수분함량을 더욱 높일 수 있는 방법이다.

• 한국가시화정보학회지
• /
• 제1권1호
• /
• pp.64-74
• /
• 2003

An experimental study was carried out to investigate the effect of periodic blowing and suction on a turbulent boundary layer. Particle image velocimetry (PIV) was used to probe the characteristics of the flow. The local forcing was introduced to the boundary layer via a sinusoidally-oscillating jet issuing from a thin spanwise slot. Three forcing frequencies (f$^{+}$=0.044, 0.066 and 0.088) with a fixed forcing amplitude (A$^{+}$=0.6) were employed at $Re_{=690. The effect of the forcing angles ($\alpha$=60$^{\circ}$ , 90$^{\circ}$ and 120$^{\circ}$ ) was investigated under the fixed forcing frequency (f$^{+}$=0.088). The PIV results showed that the wall region velocity decreases on imposition of the local forcing. Inspection of phase-averaged velocity profiles revealed that spanwise large-scale vortices were generated in the downstream of the slot and persist further downstream. The highest reduction in skin friction was achieved at highest forcing frequency (f$^{+}$=0.088) and a forcing angle of $\alpha$=120$^{\circ}$. The spatial fraction of the vortices was examined to analyze the skin friction reduction.

• 한국철도학회논문집
• /
• 제9권4호
• /
• pp.493-498
• /
• 2006

In this study, two large pile load tests were performed in the deep sand gravel deposit of Nakdong river basin so that the characteristics of the load transfer was identified. The fully instrumented rectangular barrette pile in the size of $1.5\times3.0m$ and the circular bored pile of the diameter 1.5 m were placed into the ground below 50 m. Under the applied loads of 2,400 tonf and 4,000 tonf, the test results of the load transfer showed the portion of 83% and 93% of the applied loads on the barrette pile and the bored pile, respectively, were supported by the skin friction along the pile shaft. It was revealed that the most of these skin friction mobilized in sand layer underlying clay layer having N-value more than 30 and that the friction per unit area of the bored pile was larger than the friction of barrette pile. However, if embedded in the stiff sand graval layer, the both piles were proven to be sufficient for using as the friction piles.

• Preventive Nutrition and Food Science
• /
• 제21권2호
• /
• pp.85-89
• /
• 2016

Omega-3 rich fish oils are extremely labile, thus requiring control of oxidation and off flavor development. A recently proposed emulsification method, layer-by-layer (LbL) deposition, was found to be a plausible method to enhance the characteristics of bioactive ingredients, especially lipids. The present work was designed to test the possibility of enhancing the uptake and utilization of omega-3 fatty acids present in fish oil. The bioavailability of nano-emulsified fish oil was monitored in terms of intestinal absorption as well as skin permeability by using the everted intestinal sac model and Franz cell model. The skin permeability and intestinal absorption characteristics was significantly improved by LbL emulsification with lecithin/chitosan/low methoxypectin. Multilayer encapsulation along with nano-emulsification can be a useful method to deliver biologically active lipids and related components, such as fish oil. The protective effect of this tool from lipid oxidation still needs to be verified.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2000년도 춘계학술대회논문집B
• /
• pp.472-478
• /
• 2000

An experimental study is performed to analyze flow structures behind a local suction/blowing in a flat-plate turbulent boundary layer, The local forcing is given to the boundary layer flow by means of a sinusoidally oscillating jet issuing from a thin spanwise slot at the wall. The Reynolds number based on the momentum thickness is about $Re_{\theta}=1700$. The effects of local forcing are scrutinized by altering the forcing frequency $(0.011{\leq}f^+{\leq}0.044)$. The forcing amplitude is fixed at $A_0=0.4$. It is found that a small local forcing reduces the skin friction, and this reduction increases with the forcing frequency. A phase-averaging technique is employed to capture the coherent structures. Velocity signals are decomposed into a periodic part and a fluctuating part. An organized spanwise vortical structure is generated by the local forcing. The larger reduction of skin friction for the higher forcing frequencies is attributed to the diminished adverse effect of the secondary vortex. An investigation of the random fluctuation components reveals that turbulent energy is concentrated near the center of vortical structures.

• 한국환경보건학회지
• /
• 제28권2호
• /
• pp.71-80
• /
• 2002

To evaluate the skin toxicity of topical cyclohexane application (25mg/$\textrm{cm}^2$) was sequentially applied to the rat skin for four days. On the histopathological findings in the light micrographs, neutrophils and engulfed neutrophils are seen, and many cytoplasmic processes were appeared in proliferated layer whereas in the dermis area, increased numbers of fibroblast, accumulation of neutrophil and lipid droplets are demonstrated. On the other hand, applying the cyclohexane to the rat skin led to the remarkable rise of cutaneous xanthine oxidase activity and similar activities of superoxide dismutase and glutathione peroxidase and glutathione content and declined activity of glutathione S-transferase compared with control group. Especially the remarkably decreased activity of aniline hydroxylase (AH) was appeared in skin as little as scarcely determined. Furthermore, the applying the cyclohexane to skin led to the significantly increased activity of hepatic AH and alcohol dehydrogenase. These results indicate that oxygen free radical and intermediate metabolite of cyclohexane may be responsible for structural changes in skin by cyclohexane application to rat skin.

• Journal of Pharmaceutical Investigation
• /
• 제36권5호
• /
• pp.299-304
• /
• 2006

The aim of this study was to investigate the effect of deformable liposomes with sodium cholate on the skin permeation and skin deposition of arbutin, a hydrophilic skin-whitening agent. Various compositions of liposomes were prepared by the extrusion method. Particle size distribution and entrapment efficiency were determined by the laser light scattering and the gel permeation chromatography, respectively. The in vitro rat skin permeation and deposition of arbutin in various skin layers were investigated using the Keshary-Chien diffusion cells at $37^{\circ}C$. The average particle size of the deformable liposomes ranged from 217.4 to 117.4 nm, depending on the composition. The entrapment efficiency was dependent on surfactant concentration and loading dose of arbutin. The permeation rate of 5% arbutin in deformable liposomes was $8.91({\pm}1.33){\mu}g/cm^2/h$, and was not significantly different from 5% arbutin aqueous solution $[9.82({\m}0.86){\mu}g/cm^2/h]$. The deposition of arbutin was $43.34({\pm}12.13)$ and $16.99({\pm}7.83){\mu}g/cm^2$ in stratum corneum layer and epidermis/dermis layer, respectively, after 12 h of permeation study. These results are consistent with several earlier studies for the localization effect of liposomal formulations in stratum corneum, and demonstrated the feasibility of the deformable liposomes as a promising carrier for the skin deposition of hydrophilic skin-whitening compounds.

• Biomolecules & Therapeutics
• /
• 제27권6호
• /
• pp.553-561
• /
• 2019

Rab25, a member of the Rab11 small GTPase family, is central to achieving cellular polarity in epithelial tissues. Rab25 is highly expressed in epithelial cells of various tissues including breast, vagina, cervix, the gastrointestinal tract, and skin. Rab25 plays key roles in tumorigenesis, mainly by regulating epithelial differentiation and proliferation. However, its role in skin physiology is relatively unknown. In this study, we demonstrated that Rab25 knock-out (KO) mice show a skin barrier dysfunction with high trans-epidermal water loss and low cutaneous hydration. To examine this observation, we investigated the histology and epidermal differentiation markers of the skin in Rab25 KO mice. Rab25 KO increased cell proliferation at the basal layer of epidermis, whereas the supra-basal layer remained unaffected. Ceramide, which is a critical lipid component for skin barrier function, was not altered by Rab25 KO in its distribution or amount, as determined by immunohistochemistry. Notably, levels of epidermal differentiation markers, including loricrin, involucrin, and keratins (5, 14, 1, and 10) increased prominently in Rab25 KO mice. In line with this, depletion of Rab25 with single hairpin RNA increased the expression of differentiation markers in a human keratinocyte cell line, HaCaT. Transcriptomic analysis of the skin revealed increased expression of genes associated with skin development, epidermal development, and keratinocyte differentiation in Rab25 KO mice. Collectively, these results suggested that Rab25 is involved in the regulation of epidermal differentiation and proliferation.

• 한국기계가공학회지
• /
• 제19권7호
• /
• pp.35-40
• /
• 2020

This work is concerned with various planar deformations of an isotropic sandwich beam, which generally consists of three layers: two stiff skin layers and one soft core layer. When one layer of the sandwich beam is modeled as a beam, the variational-asymptotic method is rigorously used to construct a zeroth-order beam model, which is similar to a generalized Timoshenko beam model capable of capturing the transverse shear deformations but still carries out the zeroth-order approximation. To analyze the planar sandwich beam, the sum of the energies of the two skin layers and one core layer is then formulated with different material and geometric properties and represented by a universal beam model in terms of the core-layer kinematics through interface displacement and stress continuity conditions. As a preliminary validation, two extreme examples are presented to demonstrate the capability and accuracy of this present approach.