Browse > Article

Effect of Cyclohexane Application to Rat Skin on the Skin Toxicity  

전태원 (계명대학교 공중보건학과)
조현국 ((재)경북테크노파크 경산대학교 한방생명자원특화센터 효능검증원)
윤종국 (경운대학교 안경광학과)
Publication Information
Journal of Environmental Health Sciences / v.28, no.2, 2002 , pp. 71-80 More about this Journal
Abstract
To evaluate the skin toxicity of topical cyclohexane application (25mg/$\textrm{cm}^2$) was sequentially applied to the rat skin for four days. On the histopathological findings in the light micrographs, neutrophils and engulfed neutrophils are seen, and many cytoplasmic processes were appeared in proliferated layer whereas in the dermis area, increased numbers of fibroblast, accumulation of neutrophil and lipid droplets are demonstrated. On the other hand, applying the cyclohexane to the rat skin led to the remarkable rise of cutaneous xanthine oxidase activity and similar activities of superoxide dismutase and glutathione peroxidase and glutathione content and declined activity of glutathione S-transferase compared with control group. Especially the remarkably decreased activity of aniline hydroxylase (AH) was appeared in skin as little as scarcely determined. Furthermore, the applying the cyclohexane to skin led to the significantly increased activity of hepatic AH and alcohol dehydrogenase. These results indicate that oxygen free radical and intermediate metabolite of cyclohexane may be responsible for structural changes in skin by cyclohexane application to rat skin.
Keywords
Cyclohexane; Skin; Oxygen free radical; Rat; Cyclohexane metabolizing enzyme; Morphological changes in skin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ellenhorn, M.J. and Barceloux, D.G.: Medical toxicology. pp. 968-969. Elsevier, New York, 1988
2 Nordblom, G.D. and Coon, M.J.: Hydrogen peroxide formation and stoichiometry of hydroxylation reactions catalyzed by highly purified liver microsomal cytochrome P-450. Arch Bioehem. Biophys., 180(2), 343-347, 1977   DOI   ScienceOn
3 Martin, J.P., Dailey, M. and Sugarman, E: Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem. Biophy., 255(2), 329-336, 1987   DOI   ScienceOn
4 ACGIH (American Conference of Governmental Industrial Hygienists): 1994-1995 Threshold limit values for chemical substances and physical agents and biological exposure indices. ACGIH, Cincinnati
5 Wislocki, P.G., Mima, G.T. and Anthony, Y.H.Lu.: Reactions catalyzed by the cytochrome P450 system. In 'Enzymatic Basis of Detoxication Vol. I' (Jakoby, W.B. eds.), pp. 136-143., Academic Press., New York, 1980
6 김현영, 정용현, 정재황, 서길수, 문영환: 유기용제의 피부흡수 연구. 한국산업위생학회지, 7(2), 279-288, 1997
7 Leibovitz, B.E. and Siegel, B.V.: Aspects of free radical reactions in biological system:aging. J. Gerontol., 35(1), 45-56, 1980   DOI   ScienceOn
8 윤종국, 이미경, 이상일: 성장기간이 다른 흰쥐에 사염화탄소 투여가 oxygen free radical 생성계 및 해독계 효소 활성에 미치는 영향. 한국노화학회지, 8(1), 35-42, 1998
9 Guidotti, T. L.: Principles of occupational toxicology. In 'Occupational medicine' (Zenz, C., Dickerson, O. B. and Horvath, E. P. Jr., eds.), pp. 70-84. Mosby, St. Louis Baltimore
10 Perico, A., Cassinelli, C.B,. Brugnone, F., Bavazzano, P. and Perbellini, L.: Biological monitoring of occupational exposure to cyclohexane by urinary 1,2- and l,4-cyclohexanediol determination. Int. Arch Occup. Environ. Health, 72, 115-120, 1999   DOI   ScienceOn
11 Bergmeyer, H.U.: Method of enzymatic analysis. Academic Press, New York, 2, 428-429, 1974
12 류종일, 윤종국, 신중규: 흰쥐에 있어서 Toluene 대사에 미치는 주.야 시차의 영향. 대한의생명과학회지., 5(1), 67-74, 1999
13 Smyth, H.F,Jr., Carpenter, C.P., Weil, C.S., Pozzani, U.C., Striegel, J.A. and Nycum, J.S.: Range-finding toxicity data: List Ⅶ. Am. Ind. Hyg. Assoc. J., 30(5), 470-476, 1969   DOI
14 Brown, H.S., Bishop, D.R. and Rowan, C.A.: The role of skin absorption as a route of exposure for volatile organic compounds (VOCs) in drinking water. Am. J. Public Health, 74(5), 479-484, 1984   DOI   ScienceOn
15 이혜자, 윤종국: Xylene 대사효소활성에 미치는 주.야 시차의 영향. 한국환경위생학회지., 27(2), 10-16, 2001
16 Tobby Mathias, C.G.: Occupational Dermatoses. In 'Occupational medicine' (Zenz, C., Dickerson, O. B. and Horvath, E. P. Jr., eds.), pp. 93-122, Mosby, St. Louis Baltimore
17 전태원, 이상일, 윤종국: Cyclohexane에 의한 흰쥐의 폐독성. 대한의생명과학회지, 6(4), 245-251, 2000
18 Stirpe, F. and Della Corte, E.: The regulation of rat liver xanthine oxidase. J. Biol. Chem., 244(14), 3855-3863, 1969
19 Longacre, S.L.: Cyclohexane. In 'Ehtel Browning's toxicity and metabolism of industrial solvents' (Snyder, R., ed.), pp. 225-235. Elsevier, Amsterdam, 1987
20 James, S.P. and Waring, R.H.: The metabolism of alicyclic ketones in the rabbit and rat. Xenobiotica, 1(6), 573-580, 1971   DOI   ScienceOn
21 Ellman, G.L.: Tissue sulfhydryl group. Arch Biochem Biophys, 82, 70-77, 1959   DOI   PUBMED   ScienceOn
22 Bernard, A.M., de Russis, R., Normand, J.C. and Lauwerys, R.R.: Evaluation of the subacute nephrotoxicity of cyclohexane and other industrial solvents in the female Sprague-Dawley rat. Toxieol. Lett., 45(2-3), 271-280, 1989   DOI   ScienceOn
23 Bidlack, W. R. and Lowery, G. L.: Multiple drug metabolism: p-nitroanisole reversal of acetone enhanced aniline hydroxylation. Biochem. Pharmacol., 31(3), 311-317, 1982   DOI   ScienceOn
24 Paglia, E. D. and Valentine, W. N.: Studies on the quantitative and qualitative characterization of erythrocytes glutathione peroxidase. J. Lab. Clin. Med., 70, 158-169, 1967
25 Granger, D.N. and Parks, D.A.: Role of oxygen radical in the pathogenesis of intestinal ischemia. The Physiologist, 26, 159-164, 1983
26 Perbellini, L. and Brugnone, F.: Lung uptake and metabolism of cyclohexane in shoe factory workers. Int. Arch Occup. Environ. Health, 45, 261-269, 1980   DOI
27 Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J: Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275, 1951
28 Elliott, T.H., Parke, D.U. and Williams, R.T.: Studies in detoxication. The metabolism of cyclo [$^1^4$C] hexane and its derivatives. Biochem. J., 72, 115-120, 1959   DOI
29 Niland, J.: Industrial hygiene. In Occupational medicine. (Zenz, C., Dickerson, O. B. and Horvath, E. P. Jr., eds.), pp. 1021-1022. Mosby, St. Louis Baltimore
30 Iyadomi, M., Higaki, Y, Ichiba, M., Morimoto, M. and Tomokuni, K.: Evaluation of organic solvent-induced inflammation modulated by neuropeptides in the abdominal skin of hairless rats. Ind. Health, 36(1), 40-51, 1998   DOI   ScienceOn
31 Naskali, L., Oksanen, H. and T$\"{a}$hti, H.: Astrocytes as targets for CNS effects of organic solvents in vitro. Neurotoxicology, 15(3), 609-612, 1994
32 Habig, W.H., Pabst, M.J. and Jakoby, W.B.: Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249(22), 7130-7139, 1974
33 Schefler, W.C.: Statistics for the biological sciences. pp. 84-89. Addison-Wesley, London. 1980
34 Freeman, B.A. and Crapo, J.D.: Biology of disease:free radicals and tissue injury. Lab Invest, 47(5), 412-426, 1982
35 노동부: 화학물질 및 물리적 인자의 노출기준. 노도부고시 제97-65호, 노동부, 1998
36 Edmond Riviere, J.: Absorption and Distribution. In 'Biochemical pharmacology (Hodgson, E. and Levi, P.E. eds), pp. 23-29, Appleton and Longe, Morwalk, Connectiant., 1994
37 채순님, 윤종국, 박원학: 흰쥐에 있어서 피부조직의 Oxygen Free Radical 대사계에 미치는 toluene의 영향. J. Toxicol. Pub. Health, 17(1), 33-39, 2001
38 Chow, C.K. and Tappel, A.L.: Responese of glutathione peroxidase to dietary selenium in rats. J. Nutr., 104(4), 444-451, 1974