• 한국해양바이오학회지
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• 제14권1호
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• pp.9-24
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• 2022

Low-molecular weight peptides derived from fish collagen exhibit several bioactivities, including antioxidant, antiwrinkle, antimicrobial, antidiabetic, and antihypertension effects. These peptides are also involved in triglyceride suppression and memory improvement. This study aimed to investigate the optimal processing condition for preparing low-molecular weight peptides from flounder skin, and the properties of the hydrolysate. The optimal processing conditions for peptic hydrolysis were as follows: a ratio of pepsin to dried skin powder of 2% (w/w), pH of 2.0, and a temperature of 50℃. Peptic hydrolysate contains several low-molecular weight peptides below 300 Da. Gly-Pro-Hyp(GPHyp) peptide, a process control index, was detected only in peptic hydrolysate on matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF) spectrum. 2,2'-azinobis-(3-3-ethylbenzothiazolline-6- sulfonic acid(ABTS) radical scavenging activity of the peptic hydrolysate was comparable to that of 1 mM ascorbic acid, which was used as a positive control at pH 5.5, whereas collagenase inhibition was five times higher with the peptic hydrolysate than with 1 mM ascorbic acid at pH 7.5. However, the tyrosinase inhibition ability of the peptic hydrolysate was lower than that of arbutin, which was used as a positive control. The antibacterial effect of the peptic hydrolysate against Propionibacterium acne was not observed. These results suggest that the peptic hydrolysate derived from a flounder skin is a promising antiwrinkle agent that can be used in various food and cosmetic products to prevent wrinkles caused by ultraviolet radiations.

• 대한화장품학회지
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• 제38권4호
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• pp.321-326
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• 2012

최근에는 콜라겐 펩타이드가 항상화효과를 통해 피부의 재생을 촉진시키는 효과를 한다는 연구가 보고되고 있다. 본 구는 저분자 경구 콜라겐 펩타이드가 1550 nm fractional photothermolysis 레이저 치료 후 피부재생에 미치는 영향을 알아보고자 한다. 본 실험에 참여한 환자는 총 10명으로 실험군과 대조군 각 5명씩 무작위배정되었다. 대조군과 실험군 모두 동일하게 fractional photothermolysis 레이저를 시술받았다. 실험군에서는 경구 콜라겐 펩타이드를 하루에 1,000 mg씩 총 8주간 복용하였다. 치료 전후 모든 환자에서 피부의 탄성도, 경표피수분손실도, 홍반지수를 측정하였다. 피부탄성도는 실험군에서 대조군에 비해 통계적으로 유의하게 피부의 탄력성이 증가하였음을 확인하였다(p < 0.05). 경표피수분손실도는 두군간에 유의한 차이가 없었고, 홍반지수는 실험군에서 더 빨리 감소하는 결과를 보였다. 환자의 주관적 만족도평가에서 시술 4주 후 환자의 만족도는 실험군에서 평균 2.0점, 대조군에서 1.2점으로 나타났다. 저분자 콜라겐 펩타이드는 1550 nm fractional photothermolysis 레이저 치료 후 피부회복 촉진에 도움이 된다.

• 대한한방소아과학회지
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• 제22권2호
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• pp.81-114
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• 2008

Objectives : The purpose of this study is to investigate the effect of Jeseupwiryeongtang-gagam(JWTG) on atopic dermatitis by in vivo experiment using NC/Nga atopic dermatitis mouse, which has histological and clinical similarities to the atopic dermatitis of human. Methods : To investigate the effect of JWTG on AD, we evaluated atopic dermatitis-like skin lesions by clinical skin index and analyzed immunological parameters in peripheral blood mononuclear cells(PBMCs), splenocytes, draining lymph node(DLN) and performed skin histology in ears and dorsal skin of atopic dermatitis-like skin NC/Nga mouse in vivo. Results and Conclusions : In vivo, clinical skin severity score were significantly lower in JWTG group than control group. IgE, IL-6, $TNF-{\alpha}$, IgM, IgG2a and IgG2b levels in serum were decreased remarkably in JWTG group than control group and $IFN-{\gamma}$ production, secreted in Th1 cell were increased by JWTG. After experiment ended, we analyzed immunological cells ($CD3^+$, $CD19^+$, $CD4^+$, $CD8^+$, $CD3^+$$CD69^+$, $CD4^+$$CD25^+$ and $CD49b^+$) by flow cytometry. It resulted that total absolute number of $CD3^+$, $CD19^+$, $CD4^+$ and $CD8^+$ cells were recovered as normal and $CD3^+$$CD69^+$ were decreased significantly compared with control group in isolated DLN and PBMCs from NC/Nga mouse and total absolute number of $Gr-1^+$, $CD11b^+$ and $CD3^+$ in dorsal skin of NC/Nga mouse were decreased by JWTG. We analyzed ear, DLN, and neck-back skin after biopsy and dyeing by hematoxyline/eosin(H&E) and toluidine staining (mast cells marker) and obtained results that JWTG were effective to histological symptoms (dermal and epidermal thickening, hyperkeratosis and inflammatory cell infiltration). Ear thickness was decreased significantly than the control group and the size of inflammatory lymphocytes cells(ILC) and plasma cells(PC) in DLN were also decreased.

• 한국응용과학기술학회지
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• 제38권4호
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• pp.915-921
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• 2021

본 연구에서는 피부 보습 및 진정효능을 갖는 비타민 B5인 Pantothenic acid (PA)의 피부 투과율을 개선시키기 위해 고형지질나노입자(SLN)를 설계하였다. SLN은 지질과 유화제의 종류를 변화하여 여러 조성으로 제조하였고, PA는 이중 에멀젼 가온 용융유화법으로 SLN 내에 봉입하였다. 제조 된 PA 봉입 SLN은 입자의 물리화학적 특성인 입자크기, 다분산 지수, 제타전위, 봉입율을 평가하였다. 피부 투과시험의 경우 인체 유래 인조 피부 조직을 사용하였다. 제조 된 PA 봉입 SLN의 입자크기는 192.15 - 369.87 nm이었고, 제타전위는 -21.39 - -40.55 mV이었다. SLN 내 PA의 평균 봉입효율은 44.36 - 57.16%이었고, 평균 봉입율은 12.60 - 16.36%이었다. PA 봉입 SLN의 피부 투과 결과는 SLN을 적용한 제형이 적용하지 않은 PA 용액 보다 약 3.8 - 8.8 배 PA의 피부 투과율이 개선되었다. 본 연구에서 제조 된 SLN은 PA의 피부 투과율을 개선하였음을 보여주었다. 특히, glyceryl behenate와 Span 60을 사용한 SLN이 PA의 피부 투과율이 가장 높은 결과를 보여 주었다.

• 동의생리병리학회지
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• 제32권6호
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• pp.384-395
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• 2018

The aim of this study was to investigate whether a novel formulation of an herbal extracts has an inhibitory effect on obesity. To determine its anti-obesity effects, we performed anti-obesity-related experiments in vitro and in vivo. Thus, our present study was carried out to evaluate the anti-obesity effect of herbal extracts using a high fat diet (HFD)-induced obese mouse model and 3T3-L1 adipose cells. The effects of each herbal extracts on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. Results showed that treatment with each herbal extracts at $10{\sim}100{\mu}g/ml$ had no effect on cell morphology and viability. Without evidence of toxicity, herbal extracts treatment decreased lipid accumulation compared with the untreated adipocytes controls as shown by the lower absorbance of Oil Red O stain. Futhermore, compared with control-differentiated mature adipocytes, each herbal extracts significantly inhibited lipid accumulation in mature 3T3-L1 adipocytes. In the HFD-fed obese mice, body weight, liver weight and white adipose tissue weights were significantly reduced by mixture of herbal extracts administration in mouse skin. Futhermore, we found that mixture of herbal extracts administration suppressed serum triglyceride (TG), and total cholesterol (TCHO) in HFD-induced obese mouse model. The mixture of herbal extracts of permeability was estimated by measuring the transepithelial electrical resistance (TEER) value in pig skin. The optimized formulations of herbal extracts (Test 3 formulation) showed skin permeation. However, test 1 formulation containing essential oil as enhancer showed maximum skin permeation. After confirming the enhanced skin permeability, in vivo studies were performed to assess whether skin irritation potential on the basis of a primary irritation index (PII) in rabbit skin. Reactions were scored for erythema/edema reactions at 24 h, 48 h and 72 h post-application. It was concluded that the test 1 formulation was not irritation (PII = 0). The present study suggests that the test 1 formulation might be of therapeutic interest with respect to the treatment of obesity.

• Advances in aircraft and spacecraft science
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• 제1권3호
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• pp.331-351
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• 2014

The paper presents the development of numerical models referred to a morphing actuated aileron. The structural solution adopted consists of an internal part made of a composite chiral honeycomb that bears a flexible skin with an adequate combination of flexural stiffness and in-plane compliance. The identification of such structural frame makes possible an investigation of different actuation concepts based on diffused and discrete actuators installed in the skin or in the skin-core connection. An efficient approach is presented for the development of aeroelastic condensed models of the aileron, which are used in sensitivity studies and optimization processes. The aerodynamic performances and the energy required to actuate the morphing surface are evaluated and the definition of a general energetic performance index makes also possible a comparison with a rigid aileron. The results show that the morphing system can exploit the fluid-structure interaction in order to reduce the actuation energy and to attain considerable variations in the lift coefficient of the airfoil.

• 한국산업융합학회 논문집
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• 제1권2호
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• pp.65-74
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• 1998

The purpose of this paper is to investigate the mount of relief of human solar heat load and thermal comfort in outdoor environment in summer, Six different types of sites, T garden and its neighboring area in Japan, were selected as the experiment sites. The experiments were conducted from 22 to 29 August, 1994 to find the relationship between climatic conditions and human responses, Climatic conditions, subjects's thermal sensation and skin temperature were measured. Radiant heat exchange on the human body was estimated on the basis of the measured air and surface temperature and solar radiation. Thermal index Operative Temperature and New Effective Temperature was modified with the effect of the radiant heat exchange. Human thermal comfort and skin temperature is affected by the solar radiation and the sky factor in an outdoor environment. The effect of tree shade was verified on thermal comfort, The mount of relief of human solar heat load is relation to the existence of shade a solar radiation and the sky factor. The urban garden is one of the effective design element in an urban environmental planning.

• 동의생리병리학회지
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• 제17권6호
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• pp.1543-1546
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• 2003

Psoriasis is a common inflammatory disease of the skin marked by excessive scaling associated with inflammation, affecting 1-2% of the population. Although their etiologies remain unknown, the finding of CD4+ and CD8+ lymphocytes at sites of psoriasis has led many reports to hypothesize that psoriasis is a T lymphocyte-mediated disease directed against unknown autoantigens. The oriental medicine understands the cause of psoriasis as low humidity of skin tissues and functional deteriorations of the liver and lung. Gami-Yunbueum (GY) is a mixture of two previous prescription, which were used to the protection of liver and lung. We had prescribed the GY to the three psoriasis patients for 11 weeks and examined the effectiveness of the GY by PASI (Psoriasis Area and Severity Index) score. In our experiment, GY improved the symptoms of psoriasis in all cases. These results indicated that GY can apply to the treatment of psoriasis. Furthermore, the process for treatment of psoriasis is possible to provide humidity to skin and control the functions of liver and lung.

• 대한상한금궤의학회지
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• 제8권1호
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• pp.53-65
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• 2016

Objective : The purpose of this paper is to report the improved contact dermatitis(CD) treated with the herbal medication based on Shanghanlun disease pattern identification diagnostic system. The level V steroid ointments were no use to the patient 3weeks before the treatment. Methods : In this case, the patient was diagnosed according to 'Disease Pattern Identification Diagnostic System based on Shanghanlun Provisions' and took Mahwang-tang for 36days since the final diagnosis was Taeyang-byung, number 46 provision. The photographs of patient's hands were taken to observe the process of the skin status and the discomfort from CD was measured with DLQI(Dermatologic Life Quality Index) and NRS(Numeric Rating Scale). Results : The DLQI score changed from 24 to 7 and NRS of itching changed form 8 to under 4. Conclusions : This case report shows that the herbal medication through the diagnostic system could be a remedy on CD, dealing with the skin status with no use of the level V steroid ointments. It is needed proving an alternative possibility of Korean Medicine, on other skin diseases, with later studies since there are many reports on the side-effects after some long-term steroid treatments.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 1992년도 자외선 차단 화장품의 SPF에 관한 심포지움(대한화장품학회)
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• pp.5-26
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• 1992

Many methods of in vitro SPF evaluation have been developed in order to measure SPF of sunscreen products. In this study, we compared two spectrophotometric in vitro measurements using the skin cast made of Luviset CAP- $X^{R}$ resin and Transpor $e^{R}$ tape as substrate with two in vivo measurements using guinea pig and human. And we also observed the radiation reaching on the earth's surface in Seoul for one year and the change of the primary skin irritancy along the change of SPF value. According to the our research, the first, in vivo measurement using guinea pig had the close agreement with SPF measurement using human and showed the tendancy that guinea pig SPF was lower than human SPF. The second, there was the good relationship between in vitro SPF by using Transpor $e^{R}$ tape and in vivo SPF, and it is possible to predict human SPF from Protection Index(PI) by using tape. The third, it is thought that SPE 20 is reasonable value because of UV intensity in Seoul and the primary skin irritancy of higher SPF than 25. So we propose that it is important to distinguish the sunscreen product having higher than approximate 25 from the category of general cosmetics including lower SPF sunscreen product.oduct.