Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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A Study on the Design of Solid Lipid Nanoparticles for enhanced Skin Penetration of Pantothenic Acid

Pantothenic acid의 피부 투과 개선을 위한 고형지질나노입자설계 연구

  • Yeo, Sooho (College of Pharmacy, Chung-Ang University)
  • Received : 2021.06.08
  • Accepted : 2021.08.23
  • Published : 2021.08.31
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Abstract

In this study, we designed pantothenic acid (PA) loaded solid lipid nanoparticles (SLNs) for enhanced skin penetration of PA that is used for moisturizing agent in cosmetics with hydrophilic property. SLNs were prepared using various lipids and surfactants. PA loaded SLNs were fabricated using double emulsion method. The fabricated PA loaded SLNs assessed particle size, polydispersity index, zeta potential, loading capacity. Skin penetration study was conducted using artificial skin tissue originated from human epidermis as one of the reconstructed human epidermis models. The mean particle size and zeta potential of SLNs ranged from 192.15 nm to 369.87 nm and -21.39 mV to -40.55 mV, respectively. The loading efficiency and loading amount of PA loaded SLNs were ranged from 44.36% to 57.16% and 12.60% to 16.36%, respectively. The results of penetration demonstrated that all SLNs improved PA skin penetration. In addition, the amount of PA from SLNs were approximately 3.8 - 8.8 times higher than that from PA solution. Therefore, the fabricated SLNs demonstrated the enhancment of skin penetration of PA. Particularly, the SLN, which used glyceryl behenate and Span 60, showed optimal skin penetration of PA.

본 연구에서는 피부 보습 및 진정효능을 갖는 비타민 B5인 Pantothenic acid (PA)의 피부 투과율을 개선시키기 위해 고형지질나노입자(SLN)를 설계하였다. SLN은 지질과 유화제의 종류를 변화하여 여러 조성으로 제조하였고, PA는 이중 에멀젼 가온 용융유화법으로 SLN 내에 봉입하였다. 제조 된 PA 봉입 SLN은 입자의 물리화학적 특성인 입자크기, 다분산 지수, 제타전위, 봉입율을 평가하였다. 피부 투과시험의 경우 인체 유래 인조 피부 조직을 사용하였다. 제조 된 PA 봉입 SLN의 입자크기는 192.15 - 369.87 nm이었고, 제타전위는 -21.39 - -40.55 mV이었다. SLN 내 PA의 평균 봉입효율은 44.36 - 57.16%이었고, 평균 봉입율은 12.60 - 16.36%이었다. PA 봉입 SLN의 피부 투과 결과는 SLN을 적용한 제형이 적용하지 않은 PA 용액 보다 약 3.8 - 8.8 배 PA의 피부 투과율이 개선되었다. 본 연구에서 제조 된 SLN은 PA의 피부 투과율을 개선하였음을 보여주었다. 특히, glyceryl behenate와 Span 60을 사용한 SLN이 PA의 피부 투과율이 가장 높은 결과를 보여 주었다.

Keywords

References

  1. S. H. Lee, S. K. Jeong, S. K. Ahn, "An Update of the Defensive Barrier Function of Skin", Yonsei medical journal, Vol.47. No.3 pp. 293-306, (2006) https://doi.org/10.3349/ymj.2006.47.3.293
  2. A. Ota, K. Istenic, M. Skrt, N. Segatin, N. Znidarsic, K. Kogej, N. P. Ulrih, "Encapsulation of Pantothenic Acid into Liposomes and into Alginate or Alginatepectin Microparticles loaded with Liposomes", Journal of Food Engineering, Vol.229. pp. 21-31, (2018) https://doi.org/10.1016/j.jfoodeng.2017.06.036
  3. F. B. Camargo, L. R. Gaspar, P. M. Maia Campos, "Skin Moisturizing Effects of Panthenol-based Formulations", Journal of cosmetic science, Vol.62. No.4 pp. 361- 369, (2011)
  4. H. Stettler, P. Kurka, N. Lunau, C. Manger, A. Bohling, S. Bielfeldt, K. P. Wilhelm, D. P. Stephan, D. Dahnhardt, F. H. H. Brill, H. Lenz, "A new Topical Panthenol-containing Emollient: Results from Two Randomized Controlled Studies assessing its Skin Moisturization and Barrier Restoration Potential, and the Effect on Skin Microflora", Journal of Dermatological Treatment, Vol.28. No.2 pp. 173-180, (2017) https://doi.org/10.1080/09546634.2016.1214235
  5. N. Muhamad, M. M. Yusoff, J. Gimbun, "Thermal Degradation Kinetics of Nicotinic Acid, Pantothenic Acid and Catechin derived from Averrhoa bilimbi fruits", Rsc Advances, Vol.5. No.90 pp. 74132-74137, (2015) https://doi.org/10.1039/C5RA11950B
  6. J. S. Lucks, R. H. Muller, B. Konig, "Solid lipid nanoparticles (SLN)-an Alternative Parenteral Drug Carrier System", European Journal of Pharmaceutics and Biopharmaceutics, Vol.38. No.33, (1992)
  7. U. Gonullu, M. Uner, G. Yener, E. F. Karaman, Z. Aydogmus, "Formulation and Characterization of Solid Lipid Nanoparticles, Nanostructured Lipid Carriers and Nanoemulsion of Lornoxicam for Transdermal Delivery", Acta Pharmaceutica, Vol.65. No.1 pp. 1-13, (2015) https://doi.org/10.1515/acph-2015-0009
  8. L. Mitri, R. Shegokar, S. Gohla, C. Anselmi, R. H. Muller, "Lipid Nanocarriers for Dermal Delivery of Lutein: Preparation, Characterization, Stability and Performance", International Journal of Pharmaceutics, Vol.414. No.1-2 pp. 267-275, (2011) https://doi.org/10.1016/j.ijpharm.2011.05.008
  9. C. Lotte, C. Patouillet, M. Zanini, A. Messager, R. Roguet, "Permeation and Skin Absorption: Reproducibility of Various Industrial Reconstructed Human Skin Models", Skin Pharmacology and Physiology, Vol.15. No.1 pp. 18-30, (2002) https://doi.org/10.1159/000066679
  10. D. Monti, P. Chetoni, S. Burgalassi, S. Tampucci, M Centini, C. Anselmi, "4-Methylbenzylidene Camphor Microspheres: Reconstituted Epidermis (Skinethic®) Permeation and Distribution", International journal of cosmetic science, Vol.37. No.3 pp. 298-305, (2015) https://doi.org/10.1111/ics.12199
  11. E. M. Pritchard, C. Szybala, D. Boison, D. L. Kaplan, "Silk Fibroin Encapsulated Powder Reservoirs for Sustained Release of Adenosine", Journal of Controlled Release, Vol.144. No.2 pp. 159-167, (2010) https://doi.org/10.1016/j.jconrel.2010.01.035
  12. F. Tigu, J. Zhang, G. Liu, Z Cai, Z, Y Li, "A highly active pantothenate synthetase from Corynebacterium glutamicum enables the production of d-pantothenic acid with high productivity", Applied microbiology and biotechnology, Vol.102. No.14 pp. 6039-6046, (2018) https://doi.org/10.1007/s00253-018-9017-2
  13. V. Vallez-Gomis, G. Peris-Pastor, J. L. Benede, A. Chisvert, A. Salvador, "Green determination of eight water-soluble B vitamins in cosmetic products by liquid chromatography with ultraviolet detection", Journal of Pharmaceutical and Biomedical Analysis, Vol.205. No.25 pp. 114308, (2021) https://doi.org/10.1016/j.jpba.2021.114308
  14. C. G. Acevedo-Rocha, L. S. Gronenberg, M. Mack, F. M. Commichau, H. J. Genee, "Microbial cell factories for the sustainable manufacturing of B vitamins", Current opinion in biotechnology, Vol.56. pp. 18-29 (2019) https://doi.org/10.1016/j.copbio.2018.07.006
  15. N. Muhamad, M. M. Yusoff, J. Gimbun, "Thermal degradation kinetics of nicotinic acid, pantothenic acid and catechin derived from Averrhoa bilimbi fruits", RSC advances, Vol.5. No.90 pp. 74132-74137, (2015) https://doi.org/10.1039/C5RA11950B