• Journal of Physiology & Pathology in Korean Medicine
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• v.24 no.3
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• pp.484-489
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• 2010

Allergic contact or wound induced dermatitis are common environmental health issue and seriously affect the patient's quality of life. In now, people are interested in the korean herbal bathing that get better the skin conditions. This study was performed to investigate the effect of korean herbal bathing candidates extract on allergic contact or wound induced dermatitis. ICR mice were divided into four groups: naive, control, experimental group A and B. Control and experimental groups induced allergic contact or wound induced dermatitis by DNFB and TMA. Since then, each experimental group was pasted with corresponding korean herbal bathing extract into the skin. All groups were observed after 5 days. When the korean herbal bathing extract was applied, arbitrary skin wound index and epidermal thickness were significantly decreased compared to that of control. These data suggest that these korean herbal bathing candidates may be an effective therapeutic supplements for the treatment of skin.

• Journal of the Korea Society of Computer and Information
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• v.15 no.3
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• pp.27-35
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• 2010

Accurately extracting skin color regions is very important in various areas such as face recognition and tracking, facial expression recognition, adult image identification, health-care, and so forth. In this paper, we evaluate the performances of several skin color detection algorithms in indoor environments by changing the distance between the camera and the object as well as the background colors of the object. The distance is from 60cm to 120cm and the background colors are white, black, orange, pink, and yellow, respectively. The algorithms that we use for the performance evaluation are Peer algorithm, NNYUV, NNHSV, LutYUV, and Kimset algorithm. The experimental results show that NNHSV, NNYUV and LutYUV algorithm are stable, but the other algorithms are somewhat sensitive to the changes of backgrounds. As a result, we expect that the comparative experimental results of this paper will be used very effectively when developing a new skin color extraction algorithm which are very robust to dynamic real environments.

• Journal of the Korean Applied Science and Technology
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• v.33 no.4
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• pp.706-716
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• 2016

In this study we applied the NAG obtained by the deacetylation of chitin extracted from the shells of crabs and shrimp as cosmetic ingredient. In order to compare NAG with GLC we identified the influence of cytotoxicity, anti-inflammatory, melanin biosynthesis formation inhibition on skin cell, and we measured the effects of the change of melanin and red spots. The results show that there was not any attentive cytotoxicity on the Raw 264.7 cell and B16F10 cell, and NO formation anti-inflammatory hindrance effect induced from Raw 264.7 by LPS was slight, and NAG suppressed the increase of melanin generation concentration-dependently after we induced the melanin generation with ${\alpha}$-MSH on B16F10 and measured the melanin biosynthesis inhibition. From this result, we identified the applicability of the cosmetics containing NAG as functional cosmetic for enhancing skin-lightening because when cream containing NAG was applied to skin the index of melanin red spots showed statistically meaningful changes.

• Fisheries and Aquatic Sciences
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• v.13 no.1
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• pp.1-11
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• 2010

The study was performed to optimize the processing conditions (alkali concentration, extraction time, and temperature) for rockfish skin gelatin based on yield using response surface methodology and comparison of the physicochemical properties with those of rockfish skin gelatin pretreated and extracted under ordinary conditions (alkali treatment concentration: 1.0 M; extraction time: 2 hr; extraction temperature: $60^{\circ}C$). Predicted maximum gelatin yield of 19.1% and gelatin content of 87.8% were obtained by extraction at $106.6^{\circ}C$ for 69.0 min after pretreatment with 1.1 M calcium hydroxide. Yield of gelatin extracted under high temperature/high pressure (G-HT/HP) was 54% higher than that extracted under ordinary temperature/time (G-OT/T). However, G-HT/HP was inferior in gel strength and gelling point to (G-OT/T), but comparable in transmission. Based on the physicochemical properties, G-HT/HP was unsuitable for use in products requiring higher physical properties, but could be useful for health-functional foods.

• CELLMED
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• v.11 no.1
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• pp.2.1-2.8
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• 2021

The clinical condition Amnesia causes difficulty in learning new information and the inability to recall past events. It is primarily concerned with recent memory loss. Majoon-e-Nisyan (MJN) is a polyherbal Unani formulation, present in a semi-solid form. It is widely used potent drug of the Unani System of Medicine (USM) for treating Nisyan (amnesia). In the present study polyherbal Unani formulation, MJN has been studied for its quality control and acute toxicity. Standardization (quality control) of drugs deals with drug identity, drug quality and purity determination. Standardization of MJN had been done as per the Unani pharmacopoeial parameters approved by World Health Organization (WHO) - Pharmacognostical parameters, Physico-chemical parameters, high-performance thin-layer chromatography (HPTLC), microbial load, aflatoxin, and heavy metals. Solvents and chemicals used in the study were of analytical grade and used instrument were calibrated. By conducting an acute oral toxicity study in rats, the safety of MJN was assessed. The limit test method of OECD guideline 425 was followed in the study. Results of standardization and standard operating procedures (SOPs) for preparation of MJN may serve as the standard reference in the future. The data generated in the study for the quality control of MJN proved the quality of formulation and shows that MJN is not toxic in rats following acute dosing up to 2000 mg/kg bw. The data obtained in the paper for MJN may be used as a standard guideline for preparation of the formulation which can save time, cost, and resources for future research endeavours.

• Journal of Veterinary Science
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• v.23 no.5
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• pp.73.1-73.8
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• 2022

Background: Lumpy skin disease (LSD), a disease transmitted by direct and indirect contact with infected cattle, is caused by the Lumpy skin disease virus (LSDV). The disease affects cattle herds in Africa, Europe, and Asia. The clinical signs of LSD range from mild to the appearance of nodules and lesions in the skin leading to severe symptoms that are sometimes fatal with significant livestock economic losses. Objectives: This study aimed to characterize LSDV strains in the blood of infected cattle in Thailand based on the GPCR gene and determine the phylogenetic relationship of LSDV Thailand isolates with published sequences available in the database. Methods: In total, the blood samples of 120 cattle were collected from different farms in four provinces in the northeastern part of Thailand, and the occurrence of LSDV was examined by PCR based on the P32 antigen gene. The genetic diversity of LSDV based on the GPCR gene was analyzed. Results: Polymerase chain reaction assays based on the P32 antigen gene showed that 4.17% (5/120) were positive for LSDV. All positive blood samples were amplified successfully for the GPCR gene. Phylogenetic analysis showed that LSDV Thailand isolates clustered together with LSDVs from China and Russia. Conclusions: The LSD outbreak in Thailand was confirmed, and a phylogenetic tree was constructed to infer the branching pattern of the GPCR gene from the presence of LSDV in Thailand. This is the first report on the molecular characterization of LSDV in cattle in Thailand.

• Journal of the Korean Society of Radiology
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• v.17 no.1
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• pp.63-69
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• 2023

In head and neck radiation therapy, the thermoplastic immobilization mask used for fixing the patient's posture and reproducibility causes scattered rays by being in close contact with the skin. To investigate the increase in skin dose due to the scattered rays generated from the immobilization mask, we evaluated dose reduction by decreasing contact between face skin and immobilization mask in computerized radiotherapy planning system with CT scanned images. In addition, to confirm the reproducibility problem of the setup due to the decrease in the cover area of immobilizing, the difference of each setup was confirmed using DRR and CT images. As the mask area covered for immobilizing was reduced, the dose on the skin surface significantly decreased, and it was confirmed that there was no significant difference in reproducibility even if the entire face was not covered and fixed.

• Korean Journal of Veterinary Service
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• v.45 no.2
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• pp.133-137
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• 2022

Lumpy skin disease (LSD) is a severe cross-border infectious disease that causes fever, skin and visceral nodules in cattle. LSD is caused by the lumpy skin disease virus (LSDV), a dsDNA virus that belongs to the genus Capripoxvirus. Although LSD has been found only in Southern Africa traditionally, in the last decade it is spreading very quickly through the Middle East and into Eastern Europe and China. It usually affects cattle and water buffalos being transmitted by blood-feeding insects. As it causes a huge economic impact, LSD is a notifiable disease by World Organisation for Animal Health, and managed as the legal infectious disease class I in Korea. Therefore, the purpose of this study was to confirm the existence of LSDV antigens or antibodies in Korean livestock. We collected 1,200 blood samples from cattle (Korean native and dairy cattle) and Korean water deer in 4 major provinces of the country, then tested the existence of LSDV antigen and antibody. None (0.0%) of the 1,200 blood samples were positive for both antigen and antibody of LSDV. To the best of our knowledge, this is the first study that examines the prevalence of LSDV in Korea. Our study aims to report the LSDV occurrence situation obtained by surveillance in Korea and provide information that may help prevention of LSD epidemics.

• The Korea Journal of Herbology
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• v.24 no.1
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• pp.133-139
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• 2009

Objectives : This study was designed to investigate the effects of alcoholic fermentation extract from ovary and rind of pear on human skin. Methods : We investigated the effects of samples on human skin in terms of clinical trial. 32 of healthy volunteers, who lived in mokpo participated in clinical trial. Effects of sample on hygroscopicity and water-holding capacity were investigated. Sebum, moisture, scabrousness and desquamation were also investigated. Facial massages were conducted 8 times in each volunteers. Results : Single treatment group showed elevated levels of hygroscopicity (Normal : 106.4$\pm$2.1%, vs Pear : 114.1$\pm$6.2) and water-holding capacity (Normal : 87.1$\pm$4.4%, vs Pear : 96.2$\pm$1.3) on forearm of 16 volunteers respectively. In addition, massage group using sample showed elevated moisture (Control : 3.8$\pm$5.8%, vs Sample : 15.2$\pm$4.7%) and lowered scabrousness levels (Control : -12.3$\pm$4.4%, vs Sample : -22.0$\pm$5.2%) significantly. However, the levels of sebum did not affected by sample. Conclusions : These result suggest that alcoholic fermentation extract from ovary and rind of pear has moisturizing effect on human skin.

• Journal of Ginseng Research
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• v.48 no.2
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• pp.211-219
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• 2024

Background: Recently, plant-derived exosome-like nanoparticles (PDENs) have been isolated, and active research was focusing on understanding their properties and functions. In this study, the characteristics and molecular properties of ginseng root-derived exosome-like nanoparticles (GrDENs) were examined in terms of skin protection. Methods: HPLC-MS protocols were used to analyze the ginsenoside contents in GrDENs. To investigate the beneficial effect of GrDENs on skin, HaCaT cells were pre-treated with GrDENs (0-2 × 10⁹ particles/mL), and followed by UVB irradiation or H₂O₂ exposure. In addition, the antioxidant activity of GrDENs was measured using a fluorescence microscope or flow cytometry. Finally, molecular mechanisms were examined with immunoblotting analysis. Results: GrDENs contained detectable levels of ginsenosides (Re, Rg1, Rb1, Rf, Rg2 (S), Gyp17, Rd, C-Mc1, C-O, and F2). In UVB-irradiated HaCaT cells, GrDENs protected cells from death and reduced ROS production. GrDENs downregulated the mRNA expression of proapoptotic genes, including BAX, caspase-1, -3, -6, -7, and -8 and the ratio of cleaved caspase-8, -9, and -3 in a dose-dependent manner. In addition, GrDENs reduced the mRNA levels of aging-related genes (MMP2 and 3), proinflammatory genes (COX-2 and IL-6), and cellular senescence biomarker p21, possibly by suppressing activator protein-1 signaling. Conclusions: This study demonstrates the protective effects of GrDENs against skin damage caused by UV and oxidative stress, providing new insights into beneficial uses of ginseng. In particular, our results suggest GrDENs as a potential active ingredient in cosmeceuticals to promote skin health.