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http://dx.doi.org/10.4142/jvs.22111

Molecular identification and characterization of Lumpy skin disease virus emergence from cattle in the northeastern part of Thailand  

Seerintra, Tossapol (Faculty of Veterinary Sciences, Mahasarakham University)
Saraphol, Bhuripit (Faculty of Veterinary Sciences, Mahasarakham University)
Wankaew, Sitthichai (Laboratory of Veterinary teaching hospital of Mahasarakham University)
Piratae, Supawadee (One Health Research Unit, Faculty of Veterinary Sciences, Mahasarakham University)
Publication Information
Journal of Veterinary Science / v.23, no.5, 2022 , pp. 73.1-73.8 More about this Journal
Abstract
Background: Lumpy skin disease (LSD), a disease transmitted by direct and indirect contact with infected cattle, is caused by the Lumpy skin disease virus (LSDV). The disease affects cattle herds in Africa, Europe, and Asia. The clinical signs of LSD range from mild to the appearance of nodules and lesions in the skin leading to severe symptoms that are sometimes fatal with significant livestock economic losses. Objectives: This study aimed to characterize LSDV strains in the blood of infected cattle in Thailand based on the GPCR gene and determine the phylogenetic relationship of LSDV Thailand isolates with published sequences available in the database. Methods: In total, the blood samples of 120 cattle were collected from different farms in four provinces in the northeastern part of Thailand, and the occurrence of LSDV was examined by PCR based on the P32 antigen gene. The genetic diversity of LSDV based on the GPCR gene was analyzed. Results: Polymerase chain reaction assays based on the P32 antigen gene showed that 4.17% (5/120) were positive for LSDV. All positive blood samples were amplified successfully for the GPCR gene. Phylogenetic analysis showed that LSDV Thailand isolates clustered together with LSDVs from China and Russia. Conclusions: The LSD outbreak in Thailand was confirmed, and a phylogenetic tree was constructed to infer the branching pattern of the GPCR gene from the presence of LSDV in Thailand. This is the first report on the molecular characterization of LSDV in cattle in Thailand.
Keywords
G protein-coupled chemokine receptor; lumpy skin disease virus; epidemiology; Southeast Asia; Thailand;
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