Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular identification and characterization of Lumpy skin disease virus emergence from cattle in the northeastern part of Thailand

  • Received : 2022.04.19
  • Accepted : 2022.07.05
  • Published : 2022.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Lumpy skin disease (LSD), a disease transmitted by direct and indirect contact with infected cattle, is caused by the Lumpy skin disease virus (LSDV). The disease affects cattle herds in Africa, Europe, and Asia. The clinical signs of LSD range from mild to the appearance of nodules and lesions in the skin leading to severe symptoms that are sometimes fatal with significant livestock economic losses. Objectives: This study aimed to characterize LSDV strains in the blood of infected cattle in Thailand based on the GPCR gene and determine the phylogenetic relationship of LSDV Thailand isolates with published sequences available in the database. Methods: In total, the blood samples of 120 cattle were collected from different farms in four provinces in the northeastern part of Thailand, and the occurrence of LSDV was examined by PCR based on the P32 antigen gene. The genetic diversity of LSDV based on the GPCR gene was analyzed. Results: Polymerase chain reaction assays based on the P32 antigen gene showed that 4.17% (5/120) were positive for LSDV. All positive blood samples were amplified successfully for the GPCR gene. Phylogenetic analysis showed that LSDV Thailand isolates clustered together with LSDVs from China and Russia. Conclusions: The LSD outbreak in Thailand was confirmed, and a phylogenetic tree was constructed to infer the branching pattern of the GPCR gene from the presence of LSDV in Thailand. This is the first report on the molecular characterization of LSDV in cattle in Thailand.

Keywords

Acknowledgement

This research project was financially supported by Mahasarakham University 2021.

References

  1. Weiss KE. Lumpy skin disease virus. In: Hanshaw JB, Plowright W, Weiss KE, editors. Cytomegaloviruses. Rinderpest Virus. Lumpy Skin Disease Virus. Berlin, Heidelberg: Springer; 1968, 111-131.
  2. Tulman ER, Afonso CL, Lu Z, Zsak L, Kutish GF, Rock DL. Genome of lumpy skin disease virus. J Virol. 2001;75(15):7122-7130. https://doi.org/10.1128/JVI.75.15.7122-7130.2001
  3. Irons PC, Tuppurainen ES, Venter EH. Excretion of lumpy skin disease virus in bull semen. Theriogenology. 2005;63(5):1290-1297. https://doi.org/10.1016/j.theriogenology.2004.06.013
  4. Sharawi SS, Abd El-Rahim IH. The utility of polymerase chain reaction for diagnosis of lumpy skin disease in cattle and water buffaloes in Egypt. Rev Sci Tech. 2011;30(3):821-830. https://doi.org/10.20506/rst.30.3.2075
  5. Beard PM. Lumpy skin disease: a direct threat to Europe. Vet Rec. 2016;178(22):557-558. https://doi.org/10.1136/vr.i2800
  6. Sprygin A, Pestova Y, Wallace DB, Tuppurainen E, Kononov AV. Transmission of lumpy skin disease virus: as short review. Virus Res. 2019;269:197637. https://doi.org/10.1016/j.virusres.2019.05.015
  7. Albayrak H, Ozan E, Kadi H, Cavunt A, Tamer C, Tutuncu M. Molecular detection and seasonal distribution of lumpy skin disease virus in cattle breeds in Turkey. Med Weter. 2018;74(3):175-178. https://doi.org/10.21521/mw.6081
  8. Zeedan GS, Mahmoud AH, Abdalhamed AM, El-Razik KA, Khafagi MH, Zeina HA. Detection of lumpy skin disease virus in cattle using real-time polymerase chain reaction and serological diagnostic assays in different governorates in Egypt in 2017. Vet World. 2019;12(7):1093-1100. https://doi.org/10.14202/vetworld.2019.1093-1100
  9. Ochwo S, VanderWaal K, Ndekezi C, Nkamwesiga J, Munsey A, Witto SG, et al. Molecular detection and phylogenetic analysis of lumpy skin disease virus from outbreaks in Uganda 2017-2018. BMC Vet Res. 2020;16(1):66. https://doi.org/10.1186/s12917-020-02288-5
  10. Sudhakar SB, Mishra N, Kalaiyarasu S, Jhade SK, Hemadri D, Sood R, et al. Lumpy skin disease (LSD) outbreaks in cattle in Odisha state, India in August 2019: Epidemiological features and molecular studies. Transbound Emerg Dis. 2020;67(6):2408-2422. https://doi.org/10.1111/tbed.13579
  11. Leliso SA, Bari FD, Chibssa TR. Molecular characterization of lumpy skin disease virus isolates from outbreak cases in cattle from Sawena District of Bale Zone, Oromia, Ethiopia. Vet Med Int. 2021;2021:8862180.
  12. Tuppurainen ES, Oura CA. Review: lumpy skin disease: an emerging threat to Europe, the Middle East and Asia. Transbound Emerg Dis. 2012;59(1):40-48. https://doi.org/10.1111/j.1865-1682.2011.01242.x
  13. Tran HT, Truong AD, Dang AK, Ly DV, Nguyen CT, Chu NT, et al. Lumpy skin disease outbreaks in Vietnam, 2020. Transbound Emerg Dis. 2021;68(3):977-980. https://doi.org/10.1111/tbed.14022
  14. Khoo CK, Dahlan R, Desa ZM, Syarina PN, Salim SM, Barker Z, et al. Molecular detection of lumpy skin disease virus in Malaysia 2021. Int J Infect Dis. 2022;116:S64.
  15. Arjkumpa O, Suwannaboon M, Boonrawd M, Punyawan I, Laobannu P, Yantaphan S, et al. First emergence of lumpy skin disease in cattle in Thailand, 2021. Transbound Emerg Dis. 2021;68(6):3002-3004. https://doi.org/10.1111/tbed.14246
  16. Bedekovic T, Simic I, Kresic N, Lojkic I. Detection of lumpy skin disease virus in skin lesions, blood, nasal swabs and milk following preventive vaccination. Transbound Emerg Dis. 2018;65(2):491-496. https://doi.org/10.1111/tbed.12730
  17. Wolff J, Tuppurainen E, Adedeji A, Meseko C, Asala O, Adole J, et al. Characterization of a Nigerian lumpy skin disease virus isolate after experimental infection of cattle. Pathogens. 2021;11(1):1-16. https://doi.org/10.3390/pathogens11010001
  18. Selim A, Manaa E, Khater H. Molecular characterization and phylogenetic analysis of lumpy skin disease in Egypt. Comp Immunol Microbiol Infect Dis. 2021;79:101699. https://doi.org/10.1016/j.cimid.2021.101699
  19. Koirala P, Meki IK, Maharjan M, Settypalli BK, Manandhar S, Yadav SK, et al. Molecular characterization of the 2020 outbreak of lumpy skin disease in Nepal. Microorganisms. 2022;10(3):539. https://doi.org/10.3390/microorganisms10030539
  20. Le Goff C, Lamien CE, Fakhfakh E, Chadeyras A, Aba-Adulugba E, Libeau G, et al. Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination. J Gen Virol. 2009;90(Pt 8):1967-1977. https://doi.org/10.1099/vir.0.010686-0
  21. Gelaye E, Belay A, Ayelet G, Jenberie S, Yami M, Loitsch A, et al. Capripox disease in Ethiopia: genetic differences between field isolates and vaccine strain, and implications for vaccination failure. Antiviral Res. 2015;119:28-35. https://doi.org/10.1016/j.antiviral.2015.04.008
  22. Badhy SC, Chowdhury MG, Settypalli TB, Cattoli G, Lamien CE, Fakir MA, et al. Molecular characterization of lumpy skin disease virus (LSDV) emerged in Bangladesh reveals unique genetic features compared to contemporary field strains. BMC Vet Res. 2021;17(1):61. https://doi.org/10.1186/s12917-021-02751-x
  23. Hedayati Z, Varshovi HR, Mohammadi A, Tabatabaei M. Molecular characterization of lumpy skin disease virus in Iran (2014-2018). Arch Virol. 2021;166(8):2279-2283. https://doi.org/10.1007/s00705-021-05119-6
  24. Heine HG, Stevens MP, Foord AJ, Boyle DB. A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. J Immunol Methods. 1999;227(1-2):187-196. https://doi.org/10.1016/S0022-1759(99)00072-1
  25. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-98.
  26. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547-1549. https://doi.org/10.1093/molbev/msy096
  27. Das M, Chowdhury MS, Akter S, Mondal AK, Jamal M. An updated review on lumpy skin disease: perspective of Southeast Asian countries. J. Adv. Biotechnol. Exp. Ther. 2021;4(3):322-333. https://doi.org/10.5455/jabet.2021.d133
  28. Azeem S, Sharma B, Shabir S, Akbar H, Venter E. Lumpy skin disease is expanding its geographic range: a challenge for Asian livestock management and food security. Vet J. 2022;279:105785. https://doi.org/10.1016/j.tvjl.2021.105785
  29. Ghalyanchilangeroudi A, Ziafati Kafi Z, Rajeoni A, Ataii J, Sadri N, Hajizamani N, et al. Molecular detection and phylogenetic analysis of lumpy skin disease virus in Iran. Iran J Vet Med. 2021;15(2):169-173.
  30. Aerts L, Haegeman A, De Leeuw I, Philips W, Van Campe W, Behaeghel I, et al. Detection of clinical and subclinical lumpy skin disease using ear notch testing and skin biopsies. Microorganisms. 2021;9(10):2171. https://doi.org/10.3390/microorganisms9102171
  31. Tuppurainen ES, Venter EH, Coetzer JA. The detection of lumpy skin disease virus in samples of experimentally infected cattle using different diagnostic techniques. Onderstepoort J Vet Res. 2005;72(2):153-164.
  32. Babiuk S, Bowden TR, Parkyn G, Dalman B, Manning L, Neufeld J, et al. Quantification of lumpy skin disease virus following experimental infection in cattle. Transbound Emerg Dis. 2008;55(7):299-307. https://doi.org/10.1111/j.1865-1682.2008.01024.x
  33. Saltykov YV, Kolosova AA, Filonova NN, Chichkin AN, Feodorova VA. Genetic evidence of multiple introductions of lumpy skin disease virus into Saratov Region, Russia. Pathogens. 2021;10(6):716. https://doi.org/10.3390/pathogens10060716
  34. Chibssa TR, Sombo M, Lichoti JK, Adam TI, Liu Y, Elraouf YA, et al. Molecular analysis of East African lumpy skin disease viruses reveals a mixed isolate with features of both vaccine and field isolates. Microorganisms. 2021;9(6):1142. https://doi.org/10.3390/microorganisms9061142