• 한국약용작물학회지
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• 제27권2호
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• pp.143-150
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• 2019

Background: Skin is an organ that protects the human body from various environmental stimuli that can induce immune system activation. Skin aging can be largely divided into two categories: physiological aging, which is caused by the a decreased physiological function of the skin and structural changes with aging, and photoaging, which is caused by the chemical stress induced by external stimuli such as ultraviolet (UV) radiation. Methods and Results: The objective of this study was to investigate the anti-wrinkle and UV protective effect of catechin-rich green tea extract (CGTE) in activated keratinocyte (HaCaT cells) and UV-induced skin damage in hairless mice. The results showed that CGTE inhibits the tumor necrosis factor-alpha interferon-gamma ($TNF-{\alpha}+IFN-{\gamma}$)-induced expression of matrix metalloproteinase (MMP)-1 in HaCaT cells. In addition, the CGTE treatment significantly reduced wrinkle formation, epidermal thickness, collagen deposition, and transepidermal water loss in dorsal skin irradiated with UVB. However, the ${\beta}$-glucosidase activity was significantly increased. The CGTE treatment inhibits mRNA expression and enzyme activity of MMP-2 and MMP-9 in the dorsal skin irradiated with UVB. Conclusions: It is expected that CGTE can be effectively used as a functional food and cosmetic ingredient to improve skin moisture retention and reduce wrinkle formation.

• 한방안이비인후피부과학회지
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• 제29권3호
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• pp.124-133
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• 2016

Objectives : Recently, the demands for the effective and safe depigmentating and anti-aging agents of the skin have increased due to the medical, pharmaceutical and cosmetic reasons. The aim of this study is to search new materials from the traditional herbal medicines which inhibit the aging process of skin in vitro.Methods : Human dermis cell (HS68) was used to test the effect of Rhus verniciflua Stokes (RVS). 80% ethanol or water extracts were screened for their inhibitory activities against elastase. Elastase inhibition effect was tested by microplate reader instrument. And MMP-1 suppression effect of RVS was tested by western blot. These cells were investigated the viability by MTS assay. And also the inhibition effect of tyrosinase by RVS was tested.Results : RVS (final concentrstion 1 ㎎/㎖ appeared over 30% of inhibition of elastase activity. So we are investigated anti wrinkle effects of Rhus verniciflua Stokes look through MMP-1 inhibition activity, also Extracts of RVS showed higher anti-tyrosinase activity than arbutin as final concentration 1 mg/ml. These results suggest that herbal medicines could be strong potential sources of inhibition of anti-aging and whitening effects for the skin.Colclusions : RVS was the best suppressor candidate of elastase activity among other control oriental drugs. It was found that RVS did not have toxicity to cells. We found proper concentration of RVS to treat in HS68 culture and investigated not only wrinkle care effect but also whitening effect of RVS.

• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.405-412
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• 2022

Echinodorus cordifolius (L.)은 택사과(Alismataceae)에 속하는 수생 식물이다. Echinodorus cordifolius (L.)의 피부 항노화 활성에 대한 연구는 아직까지 보고되지 않았다. 따라서 본 연구의 목적은 Echinodorus cordifolius (L.)로부터 70% 에탄올 추출물 (ECEE)을 제조하고 항산화 및 hyaluronidase 억제 활성을 통해 피부 항노화 기능성 소재로서의 가능성을 확인하였다. ECEE는 EC₅₀과 IC₅₀ 값이 각각 31.4, 300, 450 ㎍/mL로 우수한 DPPH, hydrogen peroxide 소거 활성 및 hyaluronidase 억제 활성을 가지는 것으로 나타났다. ECEE는 불멸화된 인간 각질세포인 HaCaT 세포에서 1 mM H₂O₂로 유도된 산화적 스트레스에 대해 농도 의존적으로 세포 보호 효과 증가 및 세포 내 활성산소종을 생성을 억제하는 것으로 나타났다. 또한 ECEE는 hyaluronic acid (HA) 합성 효소인 hyaluronan synthase 2 발현 수준을 상향 조절하고 HA 분해 효소인 hyaluronidase 2(HYAL2)의 발현 수준을 하향 조절하여 HaCaT 세포에서 HA 생성량을 증가시켰다. 따라서 이러한 결과를 종합하면 ECEE는 항산화, hyaluronidase 억제 및 HA 생성 촉진의 우수한 잠재력을 가진다는 것을 확인하였고 이를 통해 피부 노화를 방지하기 위한 천연 기능성 화장품 소재로서의 충분한 활용 가치가 있다고 판단된다.

• 한국식품저장유통학회지
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• 제19권4호
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• pp.463-469
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• 2012

전복가공부산물인 내장의 이용가치를 향상시키고자 열수추출, 에탄올추출 등의 방법으로 유효성분을 추출하고 항산화 및 항피부노화 활성을 측정하였다. DPPH 라디칼소거능 분석결과 Tris-HCl 추출물은 $58.60{\pm}0.88%$으로 높은 라디칼소거능을 보였으며, 다음으로 에탄올추출물은 $55.40{\pm}0.62%$의 라디칼소거능을 보였다. Anti-elastase활성을 분석한 결과 에탄올추출물이 다른 시험구에 비하여 현저하게 높은 활성을 보였다. 전복내장추출물의 세포독성을 확인하기 위하여 human dermal fibroblast 세포에 대하여 MTT 시험결과 세포독성은 전혀 나타나지 않았다. 에탄올 추출물의 농도를 달리하여 human dermal fibroblast 세포에 대해pro-collagen type I 생합성능시험결과 10.0 ${\mu}g/mL$에서 $705.30{\pm}3.06$ ng/mL로 retinoic acid $453.60{\pm}2.82$ ng/mL보다 우수한 결과를 보였다. MMP-1 활성은 10 ${\mu}g/mL$에서 $45.30{\pm}0.80$ ng/mL를 보여 control의 $62.37{\pm}0.56$ ng/mL 보다 감소정도가 낮았다. 본 연구는 전복내장추출물의 항산화와 항노화활성에 관하여 최초로 시도되었으며 향후 전복내장을 이용한 다양한 연구의 기초자료로서 중요한 의미가 있다.

• 생명과학회지
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• 제32권5호
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• pp.355-361
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• 2022

멜라닌 색소는 피부색의 주요 원인이다. 멜라닌 색소는 멜라닌 세포에서 생성된 다음 각질 세포로 전달되어 결국 피부 표면에 다양한 색상을 부여한다. 많은 탈색제 및 피부 미백제가 개발되었지만, 색소 침착을 감소시키기 위한 재료에 대한 수요는 여전히 증가하고 있다. 본 연구에서 천연 화합물을 사용하여 탈색 및 피부 미백에 대한 재료를 찾으려고 시도한 결과 겨우살이(Viscum album var. coloratum) 추출물이 색소 침착을 억제할 수 있음을 발견하였다. 인간 멜라닌 세포에 겨우살이 추출물(mistletoe extracts, ME)을 처리했을 때 색소 침착이 극적으로 감소하였다. 프로모터 리포터 분석은 ME 처리가 HM3KO 흑색종 세포에서 microphthalmia-associated transcription factor (MITF), melanophilin (MLPH), tyrosinase related protein 2 (TRP-2), and tyrosinase (TYR) 유전자의 전사를 억제한다는 것을 보여주었다. 일관되게 ME는 MITF, TRP-1 및 TYR과 같은 색소 침착 관련 분자의 단백질 수준을 감소시켰다. 또한 ME는 cAMP Responsive Element Binding Protein (CREB), AKT 및 ERK의 인산화를 감소시켰다. 이러한 결과는 ME가 색소 침착과 관련된 세포 내 신호 전달의 조절을 통해 멜라닌 생성을 억제한다는 것을 시사한다. 끝으로 ME는 색소 침착에 대한 생체 내 평가 모델인 제브라피쉬 배아의 멜라닌 생성을 현저하게 억제하였다.

• 한국자원식물학회지
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• 제33권6호
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• pp.604-614
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• 2020

Chronological aging and photoaging affect appearance, causing wrinkles, pigmentation, texture changes, and loss of elasticity in the skin. Phragmites communis is a tall perennial herb used for its high nutritional value and for medicinal purposes, such as relief from fever and vomiting and facilitation of diuresis. In this study, we investigated the effects of ethanol extract of P. communis rhizome (PCE) on skin aging. The total flavonoid and total phenolic content in PCE were 2.92 ± 0.007 ㎍ of quercetin equivalents (QE) and 231.8 ± 0.001 ㎍ of gallic acid equivalents (GAE) per 100 mg of dried extract (n = 3). The half-maximal inhibitory concentration (IC50) values of PCE for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and hydrogen peroxide scavenging activities were 0.96 and 0.97 mg/mL, respectively. PCE showed inhibitory effects on tyrosinase when L-tyrosine (IC50 = 1.25 mg/mL) and L-3,4-dihydroxyphenylalanine (IC50 = 0.92 mg/mL) were used as substrates. PCE treatment up to 200 ㎍/mL for 24 h did not cause any significant cytotoxicity in B16F10 melanocytes, human dermal fibroblasts (HDFs), and HaCaT keratinocytes. In B16F10 melanocytes, PCE (25 and 50 ㎍ /mL) inhibited melanin production and cellular tyrosinase activity after challenge with α-melanocyte-stimulating hormone (α-MSH; p < 0.05). In HDFs, PCE suppressed the mRNA expression of matrix metalloproteinase-1 (MMP-1) and reduced the activity of elastase (p < 0.05). In addition, ultraviolet B (UVB)-mediated downregulation of hyaluronic acid synthase-2 gene expression in HaCaT keratinocytes was also effectively suppressed by PCE treatment. Overall, our results showed that PCE has potential anti-skin aging activity associated with the suppression of hyperpigmentation, wrinkle formation, and reduction in dryness. PCE is a promising candidate for the development of an anti-skin aging cosmetic ingredient.

• 대한화장품학회지
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• 제30권4호
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• pp.445-448
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• 2004

노화는 크게 내인성 노화와 광노화로 분류된다. 내인성 노화는 시간이 지남에 따라 진행되는 자연적인 노화이며, 광노화는 자외선에 우리 몸이 노출되면서 발생되는 노화 현상으로 주로 피부에서 잘 나타난다. 대표적인 노화 기작으로는 체내 활성 산소의 증가로 인한 생체 구성 성분의 퇴화를 들 수 있다. 따라서, 효과적인 노화 방지를 위해서는 활성 산소를 억제할 수 있는 항산화제를 지속적으로 공급해 주어야 한다. 본 논문에서는 천연 식물 성분인 예덕나무 피 추출물이 노화 방지에 매우 우수한 효과가 있다는 실험 결과들을 보여준다. 먼저, 예덕나무 피 추출물(Mallotus japonicus bark extracts)은 hydroxy radical scavenging activity와 SOD like activity를 가지고 있으며 과산화 수소에 의해 발생하는 피부 손상을 억제하는 효과가 매우 뛰어나다. 또한 광노화 방지 효과도 매우 뛰어나 자외선 조사에 의해 발생할 수 있는 피부 세포 손상을 억제하여 주며 자외선에 의한 유전자 변이도 억제해 주는 것으로 나타났다. 결론적으로, 예덕나무 피 추출물은 피부에서 일어날 수 있는 노화 현상을 억제하는데 매우 뛰어난 효과를 가진 물질로서 화장품 원료로서의 이용 가능성이 매우 높다.

• 한국약용작물학회지
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• 제28권5호
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• pp.331-338
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• 2020

Background: The aim of this study was to analyze the total phenol and total flavonoid contents and antioxidant activity of steam-treated leaves and rhizomes of Polygonatum sibiricum Redoute. In addition, we aimed to confirm their potential use as cosmetic materials by investigating their anti-aging and skin-whitening activity. Methods and Results: The leaves and rhizomes of P. sibiricum were treated with steam at different temperatures for different durations, and the antioxidant activity (DPPH and ABTS radical scavenging activity) and total phenol and total flavonoid contents of each sample were tested. The steam temperature and treatment duration siginificantly affected the antioxidant activity and, total phenol and total flavonoid content of the leaves and rhizome of P. sibiricum. Treating the P. sibiricum samples with steam at 120℃ for 12 h, yielded higher total phenol and total flavonoid contents. Comparatively, the samples treated with steam at 120℃ for 12 to 24 h showed significantly higher antioxidant activity. Further, the steamed samples of P. sibiricum demonstrated collagenase and tyrosinase inhibition activity, which indicated their anti-aging and skin-whitening properties. The samples steamed at 120℃ for 12 h, exhibited higher collagenase and tyrosinase inhibition activity. Conclusions: Leaves and rhizomes of P. sibiricum steamed at 120℃ for 12 h, showed highest antioxidant activity and, total phenol and total flavonoid contents than all other samples. Our results indicate the potential of using P. sibiricum as a cosmetic material by confirming its excellent anti-aging and whitening activity.

• 대한본초학회지
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• 제26권1호
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• pp.111-117
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• 2011

Objectives : The present study was designed to investigate effects of mixed extract from korean medicinal herbs (MIX) on oxidation/reduction reaction-related and aging-related enzyme in vitro. Methods : We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase inhibition assay. Results : Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. The MIX showed 97% inhibition of collagenase activity, and 64% inhibition of elastase activity at 1 mg/ml concentration of MIX, next only to positive control, which indicate good efficacy for anti-wrinkle ingredient. Also it's treatment showed 34% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of MIX. Antioxidant activities were determined by DPPH radical scavenging, xanthine oxidase (XO) inhibiting activity and SOD-like activity. Also these scavenging, XO-inhibiting and SOD-like activities were measured in 91%, 80%, and 63% inhibition, respectively, at a treated dose of 1 mg/ml, compare to control. Conclusions : These results suggest that possibility of mixed korean medicinal herbs as a functional ingredient for anti-wrinkle and whitening, anti-oxidation and anti-aging cosmetic formula.

• 동의생리병리학회지
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• 제25권3호
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• pp.425-430
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• 2011

This study evaluated the effect of extraction conditions of yam (Dioscorea aimadoimo) on antioxidant, moisturizing, collagenase activity, proliferation, and migration. Yam has been recognized as a healthy food due to its various biological activities, such as anti-obesity, anti-constipation, anti-mutagenic activities, as well as its ability to decrease blood glucose and cholesterol levels. Electron donating ability of high temperature ethanol extract of Dioscorea aimadoimo (HDA) had shown 70.6% at 400 mg/ml, and low temperature ethanol extract of Dioscorea aimadoimo (LDA) had shown 40% at 400 mg/ml. SOD-like activities of LDA and HDA were 23% and 34% at 400 mg/ml respectively. LDA significantly reduced the activity of collagenase in a dose-dependent manner, which was higher than HDA. The water contents in LDA-treated skin and HDA-treated skin were increased by 45.63% and 38.65% than the placebo cream respectively. The cellular proliferation of human dermal fibroblast neonatal (HDFn) was evaluated by MTT and cell migration assay. Compared to control, the cell proliferation was elevated to 109.7% and 114% by the treatment of LDA and HDA respectively at the concentration of 200 mg/ml. In addition, LDA and HDA were induced cell migration in HDFn. Our study suggests that LDA and HDA should be a very useful cosmetic ingredient, as anti-aging and skin moisturizer.