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http://dx.doi.org/10.7783/KJMCS.2019.27.2.143

Effects of Catechin-rich Green Tea Extract on the MMP-1 Activity of HaCaT Keratinocyte Cells and on UVB-induced Skin Damage in Hairless Mice  

Yang, Won Kyung (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University)
Park, Yang Chun (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Daejeon University)
Kim, Bok Kyu (Institute of Traditional Medicine and Bioscience, Daejeon University)
Choi, Jeong June (Institute of Traditional Medicine and Bioscience, Daejeon University)
Ryu, Geon Seek (AlnO Food Inc.(LTD))
Kim, Seung Hyung (Institute of Traditional Medicine and Bioscience, Daejeon University)
Publication Information
Korean Journal of Medicinal Crop Science / v.27, no.2, 2019 , pp. 143-150 More about this Journal
Abstract
Background: Skin is an organ that protects the human body from various environmental stimuli that can induce immune system activation. Skin aging can be largely divided into two categories: physiological aging, which is caused by the a decreased physiological function of the skin and structural changes with aging, and photoaging, which is caused by the chemical stress induced by external stimuli such as ultraviolet (UV) radiation. Methods and Results: The objective of this study was to investigate the anti-wrinkle and UV protective effect of catechin-rich green tea extract (CGTE) in activated keratinocyte (HaCaT cells) and UV-induced skin damage in hairless mice. The results showed that CGTE inhibits the tumor necrosis factor-alpha interferon-gamma ($TNF-{\alpha}+IFN-{\gamma}$)-induced expression of matrix metalloproteinase (MMP)-1 in HaCaT cells. In addition, the CGTE treatment significantly reduced wrinkle formation, epidermal thickness, collagen deposition, and transepidermal water loss in dorsal skin irradiated with UVB. However, the ${\beta}$-glucosidase activity was significantly increased. The CGTE treatment inhibits mRNA expression and enzyme activity of MMP-2 and MMP-9 in the dorsal skin irradiated with UVB. Conclusions: It is expected that CGTE can be effectively used as a functional food and cosmetic ingredient to improve skin moisture retention and reduce wrinkle formation.
Keywords
Catechin-rich Green Tea Extract; UV-induced Skin Damage; Metalloproteinases; Anti-wrinkle;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 Kang JH, Pack YK, Chung ST and Row KH. (1999). Extraction and purification of EGCG from green tea. Korean Journal of Biotechnology and Bioengineering. 14:517-522.
2 Khan N, Afaq F, Saleem M, Ahmad N and Mukhtar H. (2006). Targeting multiple signaling pathways by green tea polyphenol (-)-epigallocatechin-3-gallate. Cancer Research. 66:2500-2505.   DOI
3 Kondo S. (2000). The roles of cytokines in photoaging. Journal of Dermatological Science. 23:30-36.   DOI
4 Lee SJ. (2008). Novel natural products as active material for beauty food. Food Science and Industry. 40:10-18.
5 Levin J and Maibach H. (2005). The correlation between transepidermal water loss and percutaneous absorption: An overview. Journal of Controlled Release. 103:291-299.   DOI
6 Oh JH, Kim A, Park JM, Kim SH and Chung AS. (2006). Ultraviolet B-induced matrix metalloproteinase-1 and -3 secretion are mediated via PTEN/Akt pathway in human dermal fibroblasts. Journal of Cellular Physiology. 209:775-785.   DOI
7 Rutter K, Sell D, Fraser N, Obrenovich M, Zito M, Starke-Reed P and Monnier VM. (2003). Green tea extract suppresses the age-related increase in collagen cross-linking and fluorescent products in C57BL/6 mice. International Journal for Vitamin and Nutrition Research. 73:453-460.   DOI
8 Soter NA. (1990). Acute effects of ultraviolet radiation on the skin. Seminars in Dermatology. 9:11-15.
9 Steenport M, Khan KM, Du B, Barnhard SE, Dannenberg AJ and Falcone DJ. (2009). Matrix metalloproteinase(MMP)-1 and MMP-3 induce macrophage MMP-9: Evidence for the role of TNF-a and cyclooxygenase-2. Journal of Immunology. 183:8119-8127.   DOI
10 Youn HS. (2007). Anti-inflammatory effects of resveratrol, (-)-epigallocatechin-3-gallate and curcumin by the modulation of toll like receptor signaling pathways. Korean Journal of Food Science and Technology. 39:481-487.
11 Yusuf N, Irby C, Katiyar SK and Elmets CA. (2007). Photoprotective effects of green tea polyphenols. Photodermatology, Photoimmunology and Photomedicine. 23:48-56.   DOI
12 Berardesca E and Maibach HI. (1990). Transepidermal water loss and skin surface hydration in the non invasive assessment of stratum corneum function. Dermatosen in Beruf und Umwelt. Occupation and Environment. 38:50-53.
13 Boelsma E, Hendriks HF and Roza L. (2001). Nutritional skin care: Health effects of micronutrients and fatty acids. American Journal of Clinical Nutrition. 73:853-864.   DOI
14 Cabrera C, Artacho R and Gimenez R. (2006). Beneficial effects of green tea-a review. Journal of the American College of Nutrition. 25:79-99.   DOI
15 Chen ZP, Schell JB, Ho CT and Chen KY. (1998). Green tea epigallocatechin gallate shows a pronounced growth inhibitory effect on cancerous cells but not on their normal counterparts. Cancer Letters. 129:173-179.   DOI
16 Chung JH. (2003). Photoaging in Asians. Photodermatology, Photoimmunology and Photomedicine. 19:109-121.   DOI
17 Ha JS and Kim JH. (2005). Protective effect of EGCG against reactive oxygen species-induced stress. International Journal of Oral Biology. 30:77-84.
18 Choi SW and Ryu SY. (2014). Mass production of highly pure epigallocatechin gallate. Korea. Patents. 1016594230000.
19 Imokawa G, Takema Y, Yorimoto Y, Tsukahara K, Kawai M and Imayama S. (1995). Degree of ultraviolet-induced tortuosity of elastic fibers in rat skin is age dependent. Journal of Investigative Dermatology. 105:254-258.   DOI