Browse > Article
http://dx.doi.org/10.3839/jabc.2022.052

Anti-skin aging activities of ethanol extract from Echinodorus cordifolius L. in human keratinocytes  

Haeun Mun (Department of Medical Science, Soonchunhyang University)
Seung-Hong Lee (Department of Medical Science, Soonchunhyang University)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.4, 2022 , pp. 405-412 More about this Journal
Abstract
Echinodorus cordifolius (L.) is an aquatic plant in the family Alismataceae. The anti-skin aging activity of E. cordifolius (L.) has not been yet reported. Therefore, the objective of the present study was to prepare 70% ethanol extract (ECEE) from E. cordifolius (L.) and investigate their antioxidant and anti-hyaluronidase activities for confirm the potential of anti-skin aging. ECEE showed good activities of DPPH, hydrogen peroxide scavenging, and hyaluronidase inhibition, with EC50 and IC50 values of 31.4, 300, and 450 ㎍/mL, respectively. ECEE also significantly improved cell viability and inhibited intracellular reactive oxygen species dose-dependently against 1 mM hydrogen peroxide-induced oxidative stress in immortalized human keratinocytes (HaCaT cells). Furthermore, ECEE upregulated hyaluronic acid (HA)-synthesizing enzyme hyaluronan synthase 2 (HAS2) expression level, but downregulated expression level of HA-degrading enzyme hyaluronidase 2, resulting in increased HA production in HaCaT cells. Taken together, these results suggest that ECEE shows antioxidant and anti-hyaluronidase potential and could be a functional cosmetic ingredients for anti-skin aging.
Keywords
Anti-hyaluronidase; Antioxidant; Anti-skin aging activity; Echinodorus cordifolius L.; Human keratinocytes;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Nguyen JK, Masub N, Jagdeo J (2020) Bioactive ingredients in Korean cosmeceuticals: Trends and research evidence. J Cosmet Dermatol 19(7): 1555-1569. doi: 10.1111/jocd.13344    DOI
2 Faria-Silva C, Ascenso A, Costa AM, Marto J, Carvalheiro M, Ribeiro HM, Simoes S (2020) Feeding the skin: A new trend in food and cosmetics convergence. Trends Food Sci Technol 95: 21-32. doi: 10.1016/j.tifs.2019.11.015    DOI
3 Amberg N, Fogarassy C (2019) Green Consumer Behavior in the Cosmetics Market. Resources 8(3): 137. doi: 0.3390/resources8030137    DOI
4 Ahmed IA, Mikail MA, Zamakshshari N, Hussah AS (2020) Natural anti-aging skincare: role and potential. Biogerontology 21: 293-310. doi: 10.1007/s10522-020-09865-z    DOI
5 Liyanaarachchi GD, Samarasekera JKRR, Mahanama RR, Hemalal KDP (2018) Tyrosinase, elastase, hyaluronidase, inhibitory and antioxidant activity of Sri Lankan medicinal plants for novel cosmeceuticals. Ind Crops Prod 111: 597-605. doi: 10.1016/j.indcrop. 2017.11.019.    DOI
6 Cho YJ, Kim SH, Choi YJ, Lee JB (2022) Antioxidant and Anti-inflammatory activity of Colpomenia sinuosa extract. Journal of Convergence for Information Technology 12(1): 135-143. doi: 10.22156/CS4SMB.2022 12.01.135    DOI
7 Lee TB, So YK, Kim SY, Hwang JY (2020) Biological Activities of Cosmetic Material from Ten Kinds of Flower Ethanol Extracts. Korean J. Medicinal Crop Sci. 28(4): 260-275. doi: 10.7783/KJMCS.2020.28.4.260    DOI
8 Teamkao P, Thiravetyan P (2010) Phytoremediation of ethylene glycol and its derivatives by the burhead plant (Echinodorus cordifolius (L.)): Effect of molecular size. Chemosphere 81: 1069-1074. doi: 10.1016/j.chemosphere.2010.09.049    DOI
9 Bercu R (2009) Histoanatomy of Echinodorus cordifolius (L.) Griseb. (Alismataceae). Studia Univ. VG, SSV 19: 283-286 
10 Yu SR, Kim SH, Shin SM (2018) The effect of Alisma canaliculatum and Polyporus umbellatus extracts on adipogenic differentiation of human bone marrow derived mesenchymal stromal stem cells. Korean J Orient Int Med 39: 1181-1190. doi: 10.22246/jikm.2018.39.6.1181    DOI
11 Tibirica E, Almeida A, Caillleaux S, Pimenta D, Kaplan MA, Lessa MA, Figueiredo MR (2007) Pharmacological mechanisms involved in the vasodilator effects of extracts from Echinodorus grandifloras. J Ethnopharmacol 111(1): 50-55. doi: 10.1016/j.jep.2006.10.030    DOI
12 Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200    DOI
13 Muller HE (1995) Detection of hydrogen peroxide produced by microorganism on ABTS peroxidase medium. Zentralbl Bakterio Mikrobio Hyg 259: 151-158. doi: 10.1016/S0176-6724(85)80045-6    DOI
14 AOAC (1980) Official methods of analysis. 13th ed, Association of Official Analysis Chemists, Washington, DC, USA. Method 914-915 
15 Im ST, Kang NL, Kim JS, Heo SJ, Lee SH (2022) Antioxidant and anti-hyaluronidase activities of taurine-enriched enzymatic hydrolysates prepared from Turbo cornutus, a by-product of fish processing in Jeju Island. Fish Sci 88: 509-517. doi: 10.1007/s12562-022-01605-1    DOI
16 Yoon Y, Lee YM, Song S, Lee YY, Yeum KJ (2018) Black soybeans protect human keratinocytes from oxidative stress-induced cell death. Food Sci. Nutr. 6: 2423-2430. doi: 10.1002/fsn3.842    DOI
17 AK T, Gulcin I (2008) Antioxidant and radical scavenging properties of curcumin. Chem. Biol. Interact. 174(1): 27-37. doi: 10.1016/j.cbi.2008.05.003    DOI
18 Herrmann K (1989) Occurrence and content of hydroxycinnamic and hydroxybenzoic acid compounds in foods. Crit Rev Food Sci Nutr 28: 315-347. doi: 10.1080/10408398909527504    DOI
19 Mukherjee PK, Maity N, Nema NK, Sarkar BK (2011) Bioactive compounds from natural resources against skin aging. Phytomedicine 19(1): 64-73. doi: 10.1016/j.phymed.2011.10.003    DOI
20 Harman D (1981) The aging process. Proc Natl Acad Sci USA 78: 7124-7128    DOI
21 Lee SJ, Choi HR, Lee JC, Park HJ, Lee HK, Jeong JT, Lee TB (2014) The anti-aging effects of various berries in the human skin keratinocyte (HaCaT) cells. Korean J Food Sci Technol 46(2): 198-204. doi: 10.9721/KJFST.2014.46.2.198    DOI
22 Tobin DJ (2017) Introduction to skin aging. J Tissue Viability 26(1): 37-46. doi: 10.1016/j.jtv.2016.03.002    DOI
23 Miracle Uwa L (2017) The anti-aging efficacy of antioxidants. Curr Trends Biomedical Eng & Biosci. 7(4): 66-68. doi: 10.19080/CTBEB.2017.07.555716    DOI
24 Rinnerthaler M, Bischof J, Streubel MK, Trost A, Richter K (2015) Oxidative stress in aging human skin. Biomolecules 5(2): 545-589. doi: 10.3390/biom5020545    DOI
25 Ganceviciene R, Liakou AI, Theodoridis A, Makrantonaki E, Zouboulis CC (2012) Skin anti-aging strategies. Dermatoendocrinol 4(3): 308-319. doi: 10.4161/derm.22804    DOI
26 Seo SH, Choi MO (2013) Protectvie effects of Lonicerae Japonicae Flos against hydrogen peroxidase-induced oxidative stress on Human keratinocyte, HaCaT cells. Kor J Herbology 28: 57-62. doi: 10.6116/kjh.2013.28.4.57    DOI
27 Shahidi F (1992) Phenolic antioxidant.Crit Rev Food Sci Nutr 32: 67-103. doi: 10.1080/10408399209527581    DOI
28 Kim PJ, Ha HY (2018) Effect of Lonicera japonica ethanol extract on hyaluronic acid production in HaCaT cells. J Invest Cosmetol 14: 441-446. doi: 10.15810/jic.2018.14.4.005    DOI
29 Razak DLA, Jamaluddin A, Rashid NYA, Sani NA, Manan MA (2020) Assessment of Cosmeceutical Potentials of Selected Mushroom Fruitbody Extracts Through Evaluation of Antioxidant, Anti-Hyaluronidase and Anti-Tyrosinase Activity. J 3(3): 329-342. doi: 10.3390/j3030026    DOI
30 Chang TM, Tsen JH, Yen H, Yang TY, Huang HC (2017) Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes. Evid Based Complement Alternat Med 2017. doi: 10.1155/2017/8325049    DOI
31 Kim AR, Jung MC, Jeong HI, Song DG, Seo YB, Jeon YH, Park SH, Shin HS, Lee SL, Park SN (2018) Antioxidative and cellular protective effects of Lysimachia christinae hance extract and fractions. Appl Chem Eng 29: 176-184. doi: 10.14478/ace.2017.1113    DOI
32 Kolakul P, Sripanidkulchai B (2017) Phytochemicals and anti-aging potentials of the extracts from Lagerstroemia speciosa and Lagerstroemia floribunda. Ind Crops Prod 109: 707-716. doi: 10.1016/ j.indcrop.2017.09.026    DOI
33 Kang MS, Ha HY, Kim HT (2015) An experimental study on the effect of Angelica acutiloba ethanol extract on hyaluronic acid synthesis. J Korean Med Ophthalmol Otolaryngol Dermatol 28: 32-40. doi: 10.6114/jkood.2015.28.1.032   DOI