• Journal of Food Hygiene and Safety
• /
• v.37 no.5
• /
• pp.339-346
• /
• 2022

This study was performed to investigate the quality characteristics and antioxidant activity of black soybean Sunsik product with functional food ingredients and functional labeling system. We prepared black soybean Sunsik (BS) containing black beans, cereals, and vegetables. Black soybean Sunsik with nondigestible maltodextrin and calcium lactate (BSN) was prepared by adding non-digestible maltodextrin and calcium lactate to the base recipe to apply a functional labeling system. The particle size in BS was 118.00 ㎛, whereas BSN was 127.00 ㎛. The respective L, a, and b color values of BS were 73.25, 2.36, and 14.21. The respective L, a, and b values of BSN were 73.21, 2.36, and 14.31. The respective water retention capacities of BS and BSN were 241.67% and 216.33%. No significant difference was observed between BS and BSN in the three physicochemical properties described above. However, the pH of BSN was 5.45, which was significantly lower than that of BS. The total respective phenolic contents of BS and BSN were 1.75 mg GAE/g and 1.61 mg GAE/g, and total respective flavonoid contents of BS and BSN were 6.36 mg RE/g and 5.95 mg RE/g. The antioxidant capacities of BS and BSN were compared via assays of DPPH and ABTS radical scavenging activities, FRAP, and reducing power. The antioxidant activities of BS and BSN increased in a dose-dependant manner. No significant difference between BS and BSN was observed in any measure of antioxidant capacity. These results suggested that the addition of functional food ingredients (non-digestible maltodextrin and calcium lactate) did not affect the quality characteristics and antioxidant activity of black soybean Sunsik.

• Journal of International Society for Simulation Surgery
• /
• v.1 no.1
• /
• pp.37-40
• /
• 2014

Purpose For living donor liver transplantation, liver segmentation is difficult due to the variability of its shape across patients and similarity of the density of neighbor organs such as heart, stomach, kidney, and spleen. In this paper, we propose an automatic segmentation of the liver using multi-planar anatomy and deformable surface model in portal phase of abdominal contrast-enhanced CT images. Method Our method is composed of four main steps. First, the optimal liver volume is extracted by positional information of pelvis and rib and by separating lungs and heart from CT images. Second, anisotropic diffusing filtering and adaptive thresholding are used to segment the initial liver volume. Third, morphological opening and connected component labeling are applied to multiple planes for removing neighbor organs. Finally, deformable surface model and probability summation map are performed to refine a posterior liver surface and missing left robe in previous step. Results All experimental datasets were acquired on ten living donors using a SIEMENS CT system. Each image had a matrix size of $512{\times}512$ pixels with in-plane resolutions ranging from 0.54 to 0.70 mm. The slice spacing was 2.0 mm and the number of images per scan ranged from 136 to 229. For accuracy evaluation, the average symmetric surface distance (ASD) and the volume overlap error (VE) between automatic segmentation and manual segmentation by two radiologists are calculated. The ASD was $0.26{\pm}0.12mm$ for manual1 versus automatic and $0.24{\pm}0.09mm$ for manual2 versus automatic while that of inter-radiologists was $0.23{\pm}0.05mm$. The VE was $0.86{\pm}0.45%$ for manual1 versus automatic and $0.73{\pm}0.33%$ for manaual2 versus automatic while that of inter-radiologist was $0.76{\pm}0.21%$. Conclusion Our method can be used for the liver volumetry for the pre-surgery planning of living donor liver transplantation.

• Journal of Korea Port Economic Association
• /
• v.31 no.4
• /
• pp.151-168
• /
• 2015

Shippers face uncertainty and risks until the delivery of goods to the buyer (importer). To avoid these uncertainty and risks, shippers use the abroad logistics center and then try to construct continuous contract relations. From this viewpoint, this study examines the motivations of shippers to take advantage of the logistics center and perception difference for service quality according to the characteristics of shippers. For this purpose, T-test and ANOVA analyses are conducted using SPSS 21.0. The results suggest the following implications. First, there are no differences in perception regarding the motivation to take advantage of the logistics center for the size and characteristics of products. Second, the main motivating factors are maneuver to competitors and meeting buyers' demands by using the abroad logistics center. Furthermore, there is the level of perception for service quality regarding packaging and labeling in the logistics center. In contrast, the problem process and the quality of the order progress information is higher. Finally, specific logistics services are required depending on individual products because each product's characteristics are different.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• v.18 no.2
• /
• pp.269-278
• /
• 1996

To overcome the limitations of conventional microsurgical tissue transfer, experimental creation of various neo-flaps using the vessel implantation technique has been reported. We have performed some experiments of fabrication of neo-osseous flap with local vessels and iliac bone slabs to know that the flap vascularity and neo-angiogenesis are achieved enough to microtransfer. As a next step of our previous experiments, the flap viability and the histologic change between the recipient bone and neo-oseous flap was assessed after microsurgical transplantation. The flap was created on the rabbit femoral region(n=25) using femoral vessel and the iliac bone segments($2.5{\times}1.5cm$ in size). Three weeks after neovascularization, the newly formed flap was harvested and microtransferred to the mandibular defect. As a control, contralateral mandibular defect was created and reconstructed with conventional free iliac bone graft. Scintigrams of experimental group performed 3 days after microtransfer showed hot uptake, while that of control poor uptake. Histologic and vital stain labeling study revealed good bone viability and vascularity of neo-osseous flap. In conclusion, prefabricated neo-osseous flap of our model could be transferred to the recipient site with retaining the flap viability and showed advantages over the conventional bone graft in that it was living bone graft.

• Journal of the Korea Computer Graphics Society
• /
• v.17 no.4
• /
• pp.11-21
• /
• 2011

This paper proposes a method for decomposing a Hangul glyph of outline fonts into its initial, medial and final components using statistical-structural information. In a font family, the positions of components are statistically consistent and the stroke relationships of a Hangul character reflect its structure. First, we create the component histograms that accumulate the shapes and positions of the same components. Second, we make pixel clusters from character image based on pixel direction probabilities and extract the candidate strokes using position, direction, size of clusters and adjacencies between clusters. Finally, we find the best structural match between candidate strokes and predefined character model by relaxation labeling. The proposed method in this paper can be used for a study on formative characteristics of Hangul font, and for a font classification/retrieval system.

• The Korea Journal of Herbology
• /
• v.25 no.4
• /
• pp.85-93
• /
• 2010

Objectives : This study aimed at evaluation of the effects of Gastrodiae Rhizoma on brain edema and aquaporin water channel expressions in the brain. Methods : Brain edema following intracerebral hemorrhage (ICH) was induced by the stereotaxic intrastriatal injection of bacterial collagenase type VII in Sprague-Dawley rats. Then ethanol extract of Gastrodiae rhizoma was treated once a day for 3 days. Brain edema % and water contents, and cell size of neurons in the cerebral cortex were examined. Immuno-histochemistry was processed for AQP4, AQP1, and AQP9 expressions in the brain sections and area % of immuno-labeling was analyzed with image analysis. Results : 1. Ethanol extract of Gastrodiae Rhizoma reduced brain edema of ICH induced rats significantly. 2. Ethanol extract of Gastrodiae Rhizoma reduced excessive brain tissue water contents of ICH induced rats significantly. 3. Ethanol extract of Gastrodiae Rhizoma reduced cellular edema of neurons in cerebral cortex of ICH induced rats significantly. 4. Ethanol extract of Gastrodiae Rhizoma reduced AQP4 immuno-positive area % in cerebral cortex and external capsule of ICH induced rat brain significantly. 5. Ethanol extract of Gastrodiae Rhizoma reduced AQP9 immuno-positive area % in glia limitans externa of ICH induced rat brain significantly. Conclusions : These results suggest that Gastrodiae Rhizoma reveals protective effects against brain edema and cytotoxic edema of neurons by means of down-regulation of AQP4 expression in the brain.

• Reproductive and Developmental Biology
• /
• v.35 no.3
• /
• pp.341-348
• /
• 2011

Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. The aim of the present study was to locate Ski protein in rat ovaries in order to predict the possible involvement of Ski in follicular development and atresia. First, expression of c-Ski mRNA in the ovaries of adult female rats was confirmed by RT-PCR. Then, ovaries obtained on the day of estrus were subjected to immunohistochemical analysis for Ski and proliferating cell nuclear antigen (PCNA) in combination with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL). Ski was expressed in granulosa cells that were positive for TUNEL, but negative for PCNA, regardless of the shape and size of follicles. Expression of Ski in TUNEL-positive granulosa cells, but not in PCNA-positive granulosa cells, was also verified in immature hypophysectomized rats having a single generation of developing and atretic follicles by treatment with equine chorionic gonadotropin (eCG). These results indicate that Ski is profoundly expressed in the granulosa cells of atretic follicles, but not in growing follicles, and suggest that Ski plays a role in apoptosis of granulosa cells during follicular atresia.

• The Journal of the Acoustical Society of Korea
• /
• v.16 no.3
• /
• pp.64-72
• /
• 1997

In this paper, study and experiments are performed for finding recognition unit fit which can be used in large vocabulary recognition system. Specifically, a phoneme that is currently used as recognition unit and a syllable in which Korean is well characterized are selected. From comparisons of recognition experiments, the study is performed whether a syllable can be considered as recognition unit of Korean recognition system. For report of an objective result of the comparison experiment, we collected speech data of a male speaker and processed them by hand-segmentation for phoneme boundary and labeling to construct speech database. And for training and recognition based on HMM, we used HTK (HMM Tool Kit) 2.0 of commercial tool from Entropic Co. to experiment in same condition. We applied two HMM model topologies, 3 emitting state of 5 state and 6 emitting state of 8 state, in Continuous HMM on training of each recognition unit. We also used 3 sets of PBW (Phonetically Balanced Words) and 1 set of POW(Phonetically Optimized Words) for training and another 1 set of PBW for recognition, that is "Speaker Dependent Medium Vocabulary Size Recognition." Experiments result reports that recognition rate is 95.65% in phoneme unit, 94.41% in syllable unit and decoding time of recognition in syllable unit is faster by 25% than in phoneme.

• Journal of the Korean Magnetic Resonance Society
• /
• v.16 no.1
• /
• pp.34-45
• /
• 2012

Melanocortin-4 receptor (MC4R) subtype is associated with obese humans. Especially, in a patient with severe early-onset obesity, novel heterozygous mutation in the MC4R gene was detected, resulting in an exchange of aspartic acid to asparagine in $90^{th}$ amino acid residue located in the predicted second trans-membrane domain (TM2). Mutations in the melanocortin-4 receptor (MC4R) gene are the most frequent monogenic causes of severe obesity which have been described as heterozygous with loss of function. In order to compare structure difference between MC4R wild type (MC4R-TM2-wt) and mutant (MC4R-TM2-D90N), we designed both MC4R-TM2-wt and MC4R-TM2-D90N construct in pET 21b vector. In this study, we optimized high-yield purification procedure for recombinant TM2-D90N. Eluted recombinant protein was resolubilized under urea condition for thrombin cleavage reaction and we conducted the high-performance liquid chromatography (HPLC) with reverse phase column under 1% acetonitrile, 0.01% TFA buffer solution. The molecular size of purified target peptide was confirmed by Tricine-SDS page analysis. To characterize MC4R-TM2-D90N, we have performed $^{15}N$-isotope labeling of peptide using M9 media and purified labeled target peptide for hetero-nuclear NMR spectroscopy.

• Journal of the Institute of Electronics Engineers of Korea CI
• /
• v.46 no.4
• /
• pp.102-110
• /
• 2009

In this paper, we propose an object detection method for real-time in infrared (IR) images and PowerPC (PPC) and H/W design based on field programmable gate array (FPGA). An open H/W architecture has the advantages, such as easy transplantation of HW and S/W, support of compatibility and scalability for specification of current and previous versions, common module design using standardized design, and convenience of management and maintenance. Proposed background modeling for an open H/W architecture design decreases size of search area to construct a sparse block template of search area in IR images. We also apply to compensate for motion compensation when image moves in previous and current frames of IR sensor. Separation method of background and objects apply to adaptive values through time analysis of pixel intensity. Method of clutter reduction to appear near separated objects applies to median filter. Methods of background modeling, object detection, median filter, labeling, merge in the design embedded system execute in PFC processor. Based on experimental results, proposed method showed real-time object detection through global motion compensation and background modeling in the proposed embedded system.