• Korean Journal of Food Science and Technology
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• v.36 no.2
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• pp.189-195
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• 2004

Rapid and simple method was developed for simultaneous determination of vitamins A and E contents in infant formula. Vitamins A and E were extracted by PDA-HPLC with reversed phase column using organic solvent, and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with results of Food Standards Codex and AOAC method for evaluation of developed method, Vitamins A and E contents in CRM determined by developed method were within certified range of standard values. Developed method has great advantages of simple and rapid sample preparation and simultaneous determination of vitamins A and E by PDA-HPLC using reversed phase column.

• Journal of Life Science
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• v.20 no.5
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• pp.789-793
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• 2010

Artificial chromosome manipulation and amplification of single-copy yeast artificial chromosome (YAC) are usually required in order to use YACs for applications such as physical mapping and functional analysis in eukaryotes. We designed and implemented a Simultaneous YAC Manipulation-Amplification (SYMA) system that combines the copy number amplification system of YAC with a convenient YAC manipulation system. To achieve the desired split and to amplify a YAC clone-harboring plant chromosome, a pBGTK plasmid containing a conditional centromere and thymidine kinase (TK) gene was constructed as a template to amplify the splitting fragment via PCR. By splitting, new 490-kb and 100-kb split YACs containing the elements for copy number amplification were simultaneously generated from a 590-kb YAC clone. The 100-kb split YAC was then successfully amplified 14.4-fold by adding 3 mg/ml sulfanilamide and $50\;{\mu}g/ml$ methotrexate (S3/M50) as inducing substances.

• Communications of Mathematical Education
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• v.23 no.3
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• pp.761-783
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• 2009

Problem solving ability can be fostered by dealing with many different types of problems. We investigated how $5^{th}$ and $6^{th}$ graders who did not learn traditional algebraic methods might approach the word problems of simultaneous equations. This result reveals that the strategy of guess-and-check serves as a basis for elementary school students in solving simultaneous equations. A noticeable remark is that students used the guess-and-check strategy in various ways. Whereas some students changed a variable given in the problem step by step, others did in a sophisticated way focusing on the relation between two variables. Moreover, some students were able to write an equation which was not typical but meaningful and correct. This paper emphasizes the need of connections between pre-algebraic and algebraic solutions.

• Korean Journal of Head & Neck Oncology
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• v.19 no.1
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• pp.47-51
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• 2003

Background and Objectives: Oro-and pharyngocutaneous fistula after irradiation can lead to extensive wound necrosis and fetal complication such as carotid rutpure. So, there is a need to clean and cover the wound rapidly in these compromised patients. Pectoralis major myocutaneous (PMMC) flap has been presented for reconstruction of complex defects in these irradiated patients. We applied the double paddle PMMC flap for the simultaneous reconstruction of above complex defects and evaluated the it's clinical efficacy. Material and Methods: Retrospective chart review of three cases of simultaneous head and neck reconstruction after irradiation with double paddle PMMC. All patients received prior radiotherpy before development of complications. The double paddle PMMC flap was used for mucosal lining of the oral cavity and epithelial lining of external skin in 2 cases, for mucosal lining of the pharynx and epithelial lining of external skin in 1 case. Results: Considerable complications such as major flap necrosis or fistula did not occur in any of the cases, and simultaneous reconstruction was achieved in all cases without reoperations. Conclusion: Many extended defects such as oro-and pharyngocutaneous fistula involving cover and lining about the head and neck after irradiation can be rehabilitated by the double paddle pectoralis major myocutaneous flap.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.39 no.5
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• pp.579-586
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• 2019

Pilot Point Method (PPM) is one of the popular methods to search hydraulic conductivities in the inverse method using groundwater flow equations. In this study, the Simultaneous Search based Pilot Point Method (SSBM) was applied with diverse information (e.g. hydraulic heads and/or tracer concentration) applications over previously developed sensitivity based Pilot Point Method (e.g. D-optimality based Pilot Point Method: DBM). In the case of DBM, due to the minimized the variance size, tracer concentration can be recognized as a tool to control the searching space of hydraulic conductivities. SSBM reduced the procedure of hydraulic conductivity searching, though it produced more variance for exploring hydraulic conductivities. In addition, SSBM was dependent on the initial hydraulic conductivity values for search finalized hydraulic conductivities. When tracer concentration was applied, searching hydraulic conductivities was more preferable than only when hydraulic head was applied. Applications of various data for searching hydraulic conductivities is recommended as a more efficient way.

• Journal of Ginseng Research
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• v.37 no.4
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• pp.457-467
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• 2013

A quick and simple method for simultaneous determination of the 30 ginsenosides (ginsenoside Ro, Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, 20(S)-Rg2, 20(R)-Rg2, 20(S)-Rg3, 20(R)-Rg3, 20(S)-Rh1, 20(S)-Rh2, 20(R)-Rh2, F1, F2, F4, Ra1, Rg6, Rh4, Rk3, Rg5, Rk1, Rb3, Rk2, Rh3, compound Y, compound K, and notoginsenoside R1) in Panax ginseng preparations was developed and validated by an ultra performance liquid chromatography photo diode array detector. The separation of the 30 ginsenosides was efficiently undertaken on the Acquity BEH C-18 column with gradient elution with phosphoric acids. Especially the chromatogram of the ginsenoside Ro was dramatically enhanced by adding phosphoric acid. Under optimized conditions, the detection limits were 0.4 to 1.7 mg/L and the calibration curves of the peak areas for the 30 ginsenosides were linear over three orders of magnitude with a correlation coefficients greater than 0.999. The accuracy of the method was tested by a recovery measurement of the spiked samples which yielded good results of 89% to 118%. From these overall results, the proposed method may be helpful in the development and quality of P. ginseng preparations because of its wide range of applications due to the simultaneous analysis of many kinds of ginsenosides.

• Analytical Science and Technology
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• v.34 no.5
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• pp.212-224
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• 2021

The intent of the present work was to develop a simple, sensitive, accurate, precise, rapid and economical UV- spectrophotometric and reverse phase high pressure liquid chromatographic method for the simultaneous estimation of Spironolactone and Furosemide in bulk and combined tablet dosage forms. UV-Spectrophotometry was carried out by simultaneous equation method using 0.02 M potassium dihydrogen phosphate buffer pH 3.5: Acetonitrile (50:50) v/v as a solvent. The linearity range was 2-14 ㎍ mL^-1 for Spironolactone and Furosemide with a correlation coefficient > 0.99. The chromatographic separation was achieved on 250 mm × 4.6 mm, hypersil BDS C18 column with particle size 5 ㎛, by using an isocratic mixture of 0.02 M potassium dihydrogen phosphate buffer pH 3.5: Acetonitrile: tert butyl methyl ether (49:50:1) v/v/v as a solvent at a flow rate of 1 mL min^-1 and UV detection was carried out at 254 nm. The retention time were observed to be 3.666 and 6.661 minutes for Furosemide and Spironolactone respectively. The two developed methods were validated according to the ICH guidelines for accuracy, precision, linearity, LOD, LOQ and were found to be within the limits. It can be concluded that these two methods could be successfully used for the simultaneous estimation of Spironolactone and Furosemide in bulk and combined tablet dosage forms.

• Journal of the Korean Society of Radiology
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• v.15 no.6
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• pp.821-831
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• 2021

In order to calibrate energy and efficiency using the PENELOPE Code, a PENELOPE simulation was performed using a volume source. Here, we want to verify peak efficiency and usefulness by performing simultaneous measurement and correction. calculate the coincident sum correction for all volumes, first subdivide the volumes of the cylinder and the four Marinelli beakers into three heights again. Therefore, the simultaneous measurement correction coefficient in three areas and the simultaneous measurement correction coefficient for the entire volume source are calculated as output. At low energies, the j value for each source volume (50-300 ml) is small and increases significantly in the high energy range. Simulation results showed good agreement within 2.5% for all source volumes except for 50 ml and 300 ml, which were up to 4%. This means that the correction for the simultaneous measurement effect during measurement is effective. In addition. Based on this, it can be confirmed that there is an advantage to improve the detection efficiency when measuring various sources and environmental samples.

• Journal of Korean Association for Spatial Structures
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• v.19 no.3
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• pp.51-59
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• 2019

A hybrid mid-story seismic isolation system with a smart damper has been proposed to mitigate seismic responses of tall buildings. Based on previous research, a hybrid mid-story seismic isolation system can provide effective control performance for reduction of seismic responses of tall buildings. Structural design of the hybrid mid-story seismic isolation system is generally performed after completion of structural design of a building structure. This design concept is called as an iterative design which is a general design process for structures and control devices. In the iterative design process, optimal design solution for the structure and control system is changed at each design stage. To solve this problem, the integrated optimal design method for the hybrid mid-story seismic isolation system and building structure was proposed in this study. An existing building with mid-story isolation system, i.e. Shiodome Sumitomo Building, was selected as an example structure for more realistic study. The hybrid mid-story isolation system in this study was composed of MR (magnetorheological) dampers. The stiffnessess and damping coefficients of the example building, maximum capacity of MR damper, and stiffness of isolation bearing were simultaneously optimized. Multi-objective genetic optimization method was employed for the simultaneous optimization of the example structure and the mid-story seismic isolation system. The optimization results show that the simultaneous optimization method can provide better control performance than the passive mid-story isolation system with reduction of structural materials.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.3
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• pp.11-21
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• 2021

In the last few years, there is growing interest in the field of simultaneous equation estimation in finance due to the endogeneity problem caused by measurement errors, simultaneity, or omitted variables. This study aims to discuss the endogeneity problem in corporate financing decisions and investigate the interrelationship of financial decision-making such as investment decision, dividend decision, and external financing decision in Pakistan Stock Exchange (PSX) using two-stage least squares (2SLS) and generalized method of moment (GMM) estimation. The Bruech-Pagan test shows that the data has no heteroskedasticity issue and 2SLS is a better approach in the context of this study as compared to the GMM approach, and internal instruments are also sufficiently strong and valid. The three financial decision-making attributes are not jointly determined, and the dividend is influenced by one-sided investment. In the emerging stock market context, external financing and investment are not inter-related and did not affect each other. The question of whether the simultaneous equation estimation can be useful in the context of the emerging stock markets and newly-growing firms remains unanswered. The inclusive evidence shows that the theoretical link in the emerging stock market is difficult to prove like in developed stock markets.