Browse > Article

Simultaneous Determination of Vitamin A and E in Infant Formula by Rapid Extraction and HPLC with Photodiode Array Detection  

Kwak, Byung-Man (Research and Development Center, Namyang Dairy Products Corporation)
Lee, Ki-Woong (Research and Development Center, Namyang Dairy Products Corporation)
Ahn, Jang-Hyuk (Research and Development Center, Namyang Dairy Products Corporation)
Kong, Un-Young (Research and Development Center, Namyang Dairy Products Corporation)
Publication Information
Korean Journal of Food Science and Technology / v.36, no.2, 2004 , pp. 189-195 More about this Journal
Abstract
Rapid and simple method was developed for simultaneous determination of vitamins A and E contents in infant formula. Vitamins A and E were extracted by PDA-HPLC with reversed phase column using organic solvent, and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with results of Food Standards Codex and AOAC method for evaluation of developed method, Vitamins A and E contents in CRM determined by developed method were within certified range of standard values. Developed method has great advantages of simple and rapid sample preparation and simultaneous determination of vitamins A and E by PDA-HPLC using reversed phase column.
Keywords
rapid extraction; PDA-HPLC; simultaneous determination of the vitamin A and E;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Alphonse FW, Lucia AM. Improved reverse phase liquid chromatographic determination of vitamins A and D in fortified milk. J. Assoc. Off. Anal. Chem. 67: 62-64 (1984)
2 Jonathan WD, Karlene RS. Determination of Vitamins A (Retinol) and E (alpha-Tocopherol) in Foods by Liquid Chromatography : Collaborative. J. AOAC Int. 85: 424-434 (2002)
3 Kim PZ, Kim CH. A strudy on the simultaneous analysis of fatsoluble vitamins in food stuffs and vitamin products by high performance liquid chromatography. Korean J. Chem. Soc. 33: 46-54 (1989)
4 Larry GR, Willie MC, Harold Jr. CT. Simultaneous analysis of vitamins A and E in rodent feed by high-pressure liquid chromatography. J. Agric. Food. Chem. 39: 296-299 (1991)   DOI
5 Wilhelm F. Vitamins. Walter de Gruyter, New York, NY, USA (1988)
6 The Korean Nutrition Society. Recommended dietary allowances for Koreans. 7th rev. The Korea Nutrition Society, Seoul, Korea (2001)
7 William Jr. OL, Don MH, Thomas WH, James IM, Edna RY, Ronald RE, Abdel-Gawad MS. Vitamin A and vitamin E content of infant formulas produced in the United States. J. Assoc. Off. Anal. Chem. 68: 509-511 (1985)
8 Søren KJ. Retinol determination in milk by HPLC and fluorescence detection. J. Dairy Res. 61: 233-240 (1994)   DOI   ScienceOn
9 Korea Food and Drug Administration. Food Standards Codex. Korean Foods Industry Association, Seoul, Korea (2003)
10 AOAC. Official Methods of Analysis of AOAC International. 17th ed. Ch. 45. Association of Official Analytical Chemists, Gaithersbrug, MD, USA (2000)
11 Qian H, Sheng M. Simultaneous determination of fat-soluble vitamins A, D and E and pro-vitamin D2 in animal feeds by onestep extraction and high-performance liquid chromatography analysis. J. Chromatogr. A 825: 127-133 (1998)   DOI   PUBMED   ScienceOn
12 AOAC. Official Methods of Analysis of AOAC International. 17th ed. Ch. 50, pp. 1-5. Association of Official Analytical Chemists, Gaithersbrug, MD, USA (2000)
13 Soledad AH, Sonia NR, M Teresa VN, Abel MF. Determination of vitamins A and E in infant milk formula by high-performance liquid chromatography. J. Chromatogr. A 778: 243-247 (1997)   DOI   PUBMED   ScienceOn
14 Korea Food and Drug Administration. Food Code, pp. 894-897, 912-915. Korean Foods Industry Association, Seoul, Korea (2003)
15 Stephen AB, Leroy WF. Simultaneous determination of vitamin A acetate, vitamin D2 and vitamin E acetate in multivitamin mineral tablets by high performance liquid chromatography with coupled columns. Anal. Chem. 51: 641-645 (1979)   DOI   ScienceOn
16 Harvey EI. Simplified sapnification procedure for the routine determination of total vitamin E in dairy products, foods and tissues by high-performance liquid chromatography. Analyst. 113: 1217-1221 (1988)   DOI   PUBMED   ScienceOn
17 Mary CM, Ronal HS, James RK. Isomerization of retinyl palmitate using conventional lipid extraction solvents. J. Assoc. Off. Anal. Chem. 66: 746-750 (1983)
18 AOAC. Official Methods of Analysis of AOAC International. 17th ed. Association of Official Analytical Chemists, Gaithersbrug, MD, USA (2000)