• Animal Bioscience
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• 제35권1호
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• pp.105-114
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• 2022

Objective: This work was carried out to evaluate the effect of pasture (PA) feeding on buffalo meat quality compared with buffaloes reared intensively with the use of corn silage as a forage base or alternatively with polyphite meadow hay (PH). Methods: Thirty Mediterranean bull buffaloes were distributed into three experimental diet groups: maize silage (MS), PH, and PA. The animals were slaughtered at a live weight of 250 kg, and carcass and meat quality were evaluated. After 7 days of ageing, physical and chemical parameters of longissimus thoracis muscle were determined. To evaluate lipid oxidation the thiobarbituric acid reactive substances was tested at 7 and 14 days, and also the fatty acid profile was recorded by gas chromatography. Results: The PA group, even if it showed carcass parameters lower than those of the silage maize group, reported a good meat percentage (60.59% vs 58.46%, respectively) and lower fat percentage (p<0.001). PA-fed animals showed meat redness, and even if only on raw meat, shear force was higher than the others. Low values of conjugate linoleic acid, polyunsaturated fatty acids, and n-3 were reported in the silage maize group. Principal component analysis (PCA) clearly showed the influence of different diets on meat quality, and PCA1 and PCA2 explained 82% of the variability. Conclusion: Buffaloes reared on PA had meat with high nutritional value even if they showed poor carcass performance compared to the animals fed on MS. Buffaloes fed on polyphite hay were in an intermediate position, similar to grazing animals, according to the same nutritional determinations.

• Asian-Australasian Journal of Animal Sciences
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• 제9권4호
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• pp.411-420
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• 1996

Asia Triple HIn this study an attempt was made to determine the cost of producing fodders at Savar Dairy Farm (SDF). For this purpose, all seasonal fodders, such as Maize, Jowar, Cowpea and Oat and all perennial fodders, such as Para and Napier were studied. The highest acreage of land was allocated to high-land Para (33.8%) and the lowest acreage (2.7%) was devoted to Cowpea. Jowar attained the highest yield (74.2 tons) per hectare and Maize attained the lowest yield (25.8 tons/h). The highest cost per hectare was attributed to Jowar (TK. 20944.18) while the lowest cost was attributed to low-land para (TK. 10349.86). The cost of production of fodder per kilogram was the highest (TK. 0.66) for Maize and the lowest for Oat (TK. 0.24). The cost of production of low-land Para was much lower than that of high land Para. The per kilogram cost of silage production was the highest (TK. 0.71) for Maize and the lowest (TK. 0.31) was for Napier. The gross return analysis further showed that the highest net margin and B:C ratio were observed for Napier followed by Low-land Para, Jowar, Oat, Cowpea, High-land Para and Maize. Therefore, those fodders whose B:C ratios and yield/ha were higher should be allocated more area of land to stimulate increased returns to SDF in the future is suggested.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 춘계학술대회
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• pp.92-92
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• 2018

• Asian-Australasian Journal of Animal Sciences
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• 제31권9호
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• pp.1464-1473
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• 2018

Objective: To describe in-depth sequencing, the bacterial community diversity and its succession during ensiling of whole-plant maize and subsequent exposure to air. Methods: The microbial community dynamics of fermented whole-plant maize for 60 days (sampled on day 5, 10, 20, 40, 60) and subsequent aerobic exposure (sampled on day 63 after exposure to air for 3 days) were explored using Illumina Miseq sequence platform. Results: A total of 227,220 effective reads were obtained. At the genus level, there were 12 genera with relative abundance >1%, Lactobacillus, Klebsiella, Sporolactobacillus, Norank-c-cyanobacteria, Pantoea, Pediococcus, Rahnella, Sphingomonas, Serratia, Chryseobacterium, Sphingobacterium, and Lactococcus. Lactobacillus consistently dominated the bacterial communities with relative abundance from 49.56% to 64.17% during the ensiling process. Klebsiella was also an important succession bacterium with a decrease tendency from 15.20% to 6.41% during the ensiling process. The genus Sporolactobacillus appeared in late-ensiling stages with 7.70% abundance on day 40 and 5.32% on day 60. After aerobic exposure, the Lactobacillus decreased its abundance from 63.2% on day 60 to 45.03% on d 63, and Klebsiella from 5.51% to 5.64%, while Sporolactobacillus greatly increased its abundance to 28.15%. These bacterial genera belong to 5 phyla: Firmicutes (relative abundance: 56.38% to 78.43%) was dominant, others were Proteobacteria, Bacteroidetes, Cyanobacteria, and Actinobacteria. The bacterial communities clearly clustered into early-ensiling (d 5), medium-ensiling (d 10, d 20), late-ensiling (d 40, d 60), and aerobic exposure (d 63) clusters, with early- and late-ensiling communities more like each other than to the aerobic exposure communities. Conclusion: High-throughput sequencing based on 16S rRNA genes proved to be a useful method to explore bacterial communities of silage. The results indicated that the bacterial communities varied during fermentation and more dramatically during aerobic exposure. The study is valuable for understanding the mechanism of population change and the relationship between bacteria and ensilage characteristics.

• 한국작물학회지
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• 제66권4호
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• pp.392-402
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• 2021

본 연구는 남부지역 논 토양에서 옥수수(사료용 및 간식용) 재배 시 경운방법과 파종시기의 차이가 작물의 생육 및 수량에 미치는 영향을 구명하고, 여름 재배기간을 거친 후 겨울 소득작물인 양파에 미치는 영향을 구명하여 전·후작물 모두 적절한 수량을 얻을 수 있는 경운방법과 파종시기 및 파종한계기를 구명하고자 실시하였다. 1. 옥수수의 경우 경운처리에 따라서는 생육 및 수량에 유의한 차이가 없었다. 파종시기에 따라서는 광평옥, 일미찰 모두 파종시기가 늦을수록 생육이 저조하였고 착수고의 경우 생육의 차이에 의해 7월 15일 파종 처리구가 비교적 낮았다. LAI의 경우 광평옥은 7월 3일, 7월 15일 파종 처리구에서 초기 생육기 온도가 높음에 따라 엽의 발달이 빨랐고 시간이 경과됨에 따라서 유의한 차이는 없어졌다. 일미찰의 경우는 파종시기에 따라 LAI에 유의한 차이가 없었다. 일미찰은 습해에 약한 품종으로 7월 3일, 7월 15일 파종 처리구는 생육 초기 고온임에도 불구하고 장마에 의한 과습의 피해로 엽의 발달에 차이가 없었다. 2. 광평옥 수량구성요소의 경우 파종시기가 늦을수록 이삭 장 및 단위면적당 이삭수가 적었고, 이삭 및 간엽의 건물수량과 TDN도 낮았다. 일미찰 또한 수량구성요소 및 수량이 파종시기가 늦을수록 낮게 나타났다. 3. 본 실험에서 실시된 심경처리의 경우 옥수수의 생육에는 크게 영향이 없는 것으로 나타났다. 파종시기에 따라서는 파종이 지연됨에 따라 특히 7월 15일 파종 처리구에서 옥수수의 생육 및 수량이 부진하였으므로 파종시기는 7월 3일 파종 처리구와 시기가 비슷한 7월 초까지가 적절할 것으로 판단된다. 4. 양파의 경우 여름작물인 옥수수에 실시된 경운방법 및 파종시기 처리에 따라 생육 및 수량에 유의한 차이가 없었으며 양호한 생육과 수량을 보였다. 이는 2019년도 여름 재배기간을 거치고 겨울작물 재배 준비를 위한 포장정지작업이 이루어짐에 따라 양파 파종까지 4개월 이상이 경과되어 경운 및 파종시기 처리의 효과가 상쇄되어 양파의 생육에 영향이 없었던 것으로 판단된다.

• 한국작물학회지
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• 제65권4호
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• pp.406-415
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• 2020

본 연구는 간척지 내 재배 가능한 내염성 사료용 옥수수를 선발하기 위해 EMS를 이용하여 돌연변이 집단을 구축하고 기내 선발 방법을 통해 내염성 증진 계통을 선발하였고, 연구결과는 다음과 같다. 1. 사료용 옥수수 모본인 KS140에 다양한 조건으로 EMS를 처리하여 발아율 및 식물의 생육 상태를 조사하였고, 발아율에는 영향을 미치지 않으면서 생육에 큰 영향을 주지않는 0.5% EMS를 돌연변이 유도 적정 농도로 선정하였다. 2. 기내 선발 방법을 통해 선발된 내염성 증진 계통 140ES91 계통을 이용하여 0.5% 염분 스트레스 처리 후 표현형을 조사한 결과, 대조군인 KS140 대비 식물의 초장 및 근장의 생육이 양호하였으며, 높은 proline 함량과 기공전도도를 보였다. 따라서 염분 스트레스 시 높은 proline 함량과 기공전도도가 140ES91 계통의 증가된 내염성과 관련이 있을 것으로 판단된다. 3. 내염성 증진 140ES91 계통의 유전변이 분석을 통해 유전자 기능에 영향을 미칠 수 있는 아미노산 변이를 유발하는 39개의 변이 유전자를 확인하였고, 변이 유전자와 내염성의 관계를 증명하기 위한 유전자 기능 분석이 요구된다. 4. 기 보고된 내염성 관련 유전자들의 발현 양상을 조사한 결과 ABP9과 CIPK31 유전자는 대조군 대비 염분 스트레스 처리 전후 140ES91 계통에서 높은 발현율을 보였으며, CIPK21 유전자는 염분 스트레스 처리 후 대조군에는 발현이 감소하나 140ES91 계통에서는 발현이 유지됨을 확인하였다. 따라서 140ES91 계통에서 보이는 내염성 관련 유전자들의 높은 발현율이 140ES91 계통의 내염성에 관여할 것으로 판단된다. 5. 이상의 결과 기내 선발 방법을 통해 선발한 내염성 증진 계통은 간척지와 같은 염류집적 토양에서 작물의 안정적인 재배와 생산이 가능한 내염성 증진 품종 개발의 육종소재로 활용될 수 있을 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제20권3호
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• pp.378-384
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• 2007

Three microorganisms and one chemical preservative were tested for their effects on the fermentation and aerobic stability of whole-crop wheat, sorghum and maize silages. Wheat at the early dough stage, sorghum at the late milk stage and maize at the one-third milk line stage were harvested and ensiled in 1.5-l anaerobic jars untreated or after the following treatments: control (no additives); Lactobacillus plantarum (LP) at $1.0{\times}10^6$ colony-forming units (CFU)/g of fresh forage; L. buchneri (LB) at $1.0{\times}10^6$ CFU/g; Propionibacterium acidipropionici (PA) at $1.0{\times}10^6$ CFU/g; and a formic acid-based preservative (FAP) at 3 ml/kg of fresh forage weight. Three jars per treatment were sampled on d 90 after ensiling, for chemical and microbiological analysis. At the end of the ensiling period, 90 d, the silages were subjected to an aerobic stability test lasting 5 d. In this test, $CO_2$ produced during aerobic exposure was measured along with chemical and microbiological parameters which serve as spoilage indicators. The silages inoculated with LP had higher concentration of lactic acid compared with the controls and the other treated silages (p<0.05). The controls and LP-inoculated silages spoiled upon aerobic exposure faster than LB, PA and FAP-treated silages. The controls and LP-inoculated silages spoiled upon aerobic exposure faster than LB, PA and FAP-treated silages due to more $CO_2$ production (p<0.05) in these two groups and development of yeasts unlike the other groups. In the experiment, the silages treated with LB, PA and FAP were stable under aerobic conditions. However, the numbers of yeasts was higher in the LP-inoculated wheat, sorghum and maize silages compared with the LB, PA and FAP-treated silages. The LB, PA and FAP improved the aerobic stability of the silages by causing more extensive heterolactic fermentation that resulted in the silages with high levels of acetic and propionic acid. The use of LB, PA and FAP as silage additives can improve the aerobic stability of whole-crop wheat, sorghum and maize silages by inhibition of yeast activity.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.643-648
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• 2005

Near infrared reflectance spectroscopy (NIRS) has become increasingly used as a rapid, accurate method of evaluating some chemical constituents in cereal grains and forages. If samples could be analyzed without drying and grinding, then sample preparation time and costs may be reduced. This study was conducted to develop robust NIRS equations to predict fermentation quality of corn (Zea mays) silage and to select acceptable sample preparation methods for prediction of fermentation products in corn silage by NIRS. Prior to analysis, samples (n = 112) were either oven-dried and ground (OD), frozen in liquid nitrogen and ground (LN) and intact fresh (IF). Samples were scanned from 400 to 2,500 nm with an NIRS 6,500 monochromator. The samples were divided into calibration and validation sets. The spectral data were regressed on a range of dry matter (DM), pH and short chain organic acids using modified multivariate partial least squares (MPLS) analysis that used first and second order derivatives. All chemical analyses were conducted with fresh samples. From these treatments, calibration equations were developed successfully for concentrations of all constituents except butyric acid. Prediction accuracy, represented by standard error of prediction (SEP) and $R^2_{v}$ (variance accounted for in validation set), was slightly better with the LN treatment ($R^2$ 0.75-0.90) than for OD ($R^2$ 0.43-0.81) or IF ($R^2$ 0.62-0.79) treatments. Fermentation characteristics could be successfully predicted by NIRS analysis either with dry or fresh silage. Although statistical results for the OD and IF treatments were the lower than those of LN treatment, intact fresh (IF) treatment may be acceptable when processing is costly or when possible component alterations are expected.

• Asian-Australasian Journal of Animal Sciences
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• 제22권2호
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• pp.216-224
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• 2009

In Japan, since rice consumption has been decreasing with the westernization of Japanese eating habits, surplus paddy fields have been increasing. If these surplus paddy fields can be utilized for forage rice production as feed for animal production and excretions (feces and urine) from animal production can be applied to the paddy fields as manure, then the problems of surplus paddy fields and excretions from animal production may be solved, and the environment kept sustainable. The objectives of the present study were to apply a bio-economic model to dairy and forage rice integration systems in Japan and to examine the merit of introducing whole crop rice silage (WCRS), as well as economic and environmental effects of various economic and management options in the systems. Five simulations were conducted using this model. The use of WCRS as a home-grown feed increased environmental loads and decreased economic benefit because of the higher amount of purchased feed, when compared to the use of typical crops such as maize, alfalfa and timothy silage (simulation 1). Higher economic benefits from higher forage rice yields and higher milk production of a dairy cow were obtained (simulations 2, 3). There were no economic and environmental incentives for utilizing crude protein (CP) rich WCRS, because an increase in the CP content in WCRS led to the use of more chemical fertilizers, resulting in high production costs and nitrogen outputs (simulation 4). When evaluated under the situation of a fixed herd size, increasing forage rice yields decreased the total benefit of the production, in spite of the fact that the amount of subsidies per unit of land increased (simulation 5). It was indicated that excess subsidy support may not promote yield of forage rice. It was, however, observed in most cases that dairy and forage rice integration systems could not be economically established without subsidies.

• 한국작물학회지
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• 제30권3호
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• pp.277-286
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• 1985

다수다얼성 재래종 옥수수 (MET라 칭함)의 자식5세대인 3계통을 Siliage생산을 목적으로 상이한 재식밀도에서 대조품종인 수원 19호, 부여 ＃3 $\times$ ＃2를 공시하여 재배한 결과 그 성적은 다음과 같다. 1. 수확기의 초장에 대한 분산분석은 품종간에 유의차가 인정되지 않았으며, 특히 MET 계통은 교잡종이나 합성 품종에 비해 초기생육이 늦었는데 이는 MET계통이 재내종으로 자식열세 현상때문이라 추측된다. 재식밀도의 차이에 있어서 공시 품종의 초장은 큰 효과를 기대할 수 없었다. 2 수확기에서 총생체중은 MET 1이 교잡종보다 10a당 2000kg이 많았으며 특히, 밀식구인 60$\times$20cm에서 가장 높았다. 3. 단위 면적당 건물중 역시 생체량과 같은 경향의 증감으로 나타났으며, 10a부 MET 1의 총건물중은 2.4톤으로 수원 19호보다 10% 높았는데, 밀식구인 60$\times$ 20cm에서 그 효과가 컸다. 4. 10a당 종실수량은 MET 1 이 교잡종에 비해 소식구에서 낮았다. 5. MET계통의 평균 분얼수는 4.5개인 반면에 교잡종과 합성품종에서는 나타나지 않았다. 6. MET계통의 재배상의 가장 큰 문제로 도복율이 높았는데 MET계통의 착수고를 낮춤으로서 피해를 경감시킬 수 있을 것으로 생각되며 7. MET계통의 100입중은 9gr인 반면에 교잡종과 합성품종은 30gr정도이었다.