• Mycobiology
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• v.41 no.1
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• pp.37-41
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• 2013

Laccases (EC 1.10.3.2) are copper-containing polyphenol oxidases found in white-rot fungi. Here, we report the cloning and analysis of the nucleotide sequence of a new laccase gene, fvlac7, based on the genomic sequence of Flammulina velutipes. A primer set was designed from the putative mRNA that was aligned to the genomic DNA of F. velutipes. A cDNA fragment approximately 1.6-kb long was then amplified by reverse transcriptase-PCR using total RNA, which was subsequently cloned and sequenced. The cDNA sequence of fvlac7 was then compared to that of the genomic DNA, and 16 introns were found in the genomic DNA sequence. The fvlac7 protein, which consists of 538 amino acids, showed only 42~51% identity with 12 different mushroom species containing two laccases of F. velutipes, suggesting the fvlac7 is a novel laccase gene. The first 25 amino acids of Fvlac7 correspond to a predicted signal sequence, four copper-binding sites, and four N-glycosylation sites. Fvlac7 cDNA was heterologously overexpressed in an Escherichia coli system with an approximate expected molecular weight of 60 kDa.

• 전기의세계
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• v.21 no.3
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• pp.19-30
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• 1972

This paper deals with an analytic design of feedback regulator and signal state estimator in discrete linear systems. On the way of developing the deadbeat regulator, some necessary conditions for control policy have been derived, it is proved that the q periods delay in the control causes q periods delay in the point at which deadbeat response occurs. We have derived some relations such that the eigenvalue of system plant can be arbitrarily changed by the characteristics of minor loop compensator which is introduced in feedback path. And also we show that the signal state estimator which estimates the state of given signal sequence must satisfy some conditions. Theorems and conclusions are described with some simplel nontrivial numerical examples and signal state tracking application problems.

• Journal of Institute of Control, Robotics and Systems
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• v.20 no.9
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• pp.977-983
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• 2014

A repeat-back jamming signal is an intentionally re-broadcasted GNSS (Global Navigation Satellite System) interference. In this paper, a novel repeat-back jamming detection scheme is proposed. The proposed scheme uses a combined pseudo random noise signal (C-PRN) and is available for a generic GNSS receiver with a single antenna. The C-PRN signal is made by combining several received pseudo random noise signals that had been transmitted from the visible GNSS satellites. Through a Monte-Carlo simulation, the detection probability of a repeat-back jamming signal detected with the proposed scheme is presented.

• Molecules and Cells
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• v.26 no.2
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• pp.140-145
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• 2008

Expression of olive flounder hepcidin I (HepI) fused with truncated OmpA signal peptides ($OmpASP_{tr}$) as directional signals does not produce soluble fusion proteins. However, by inserting amino acid segments (xxx) varying in pI and hydrophobicity/hydrophilicity into a leader sequence containing a truncated OmpASP ($OmpASP_{tr}$) and a factor Xa cleavage site (Xa) [$OmpASP_{tr}{\mid}(xxx){\mid}Xa$], we were able in some cases to express soluble recombinant HepI. Soluble expression of the recombinant protein strongly correlated with (xxx) insertions of high pI and hydrophilicity. Therefore, we modified the $OmpASP_{tr}{\mid}(xxx){\mid}Xa$ sequence by inserting Arg and Lys into (xxx) to increase the hydrophilicity of the signal peptide region. These modifications enhanced the expression of soluble recombinant HepI. Hydropathic profile analysis of the $OmpASP_{tr}{\mid}(xxx){\mid}Xa$ HepI fusion proteins revealed that the transmembrane-like domains derived from the $OmpASP_{tr}{\mid}(xxx){\mid}Xa$ sequence were larger than the internal positively charged domain native to HepI. It should therefore be possible to overcome the obstacle of internal positively charged domains to obtain soluble expression of recombinant proteins by monitoring the hydrophilicity and hydropathic profile of the signal peptide region using a computer program.

• Investigative Magnetic Resonance Imaging
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• v.1 no.1
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• pp.66-74
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• 1997

fMRI, functional MRI introduced receently appears based on the gradient echo technique which is sensitive to the field inhomogeneity developed due to the local susceptibility changes of blood oxygenation and deoxygenation. There has been many variants of the basic gradient echo sequence which is sensitive to the local inhomogeniety, among others such as GRASS or SSFP to EPISTAR are the most commonly used gradient echo techniques. Common to all these gradient echo techniques is that the signal due to the susceptibility effects is generally decreased with increasing inhomogeneity due to the $T2^{*}$ effect or conventionally konwn as blood oxygenation level dependent(BOLD) effect. It is, also found that the BOLD sensitivity is also dependent on the imaging modes, namely whether the imaging is in axial, or coronal or sagittal mode as well as the directions of the vessels against the main magnetic field. We have, therefore, launched a systematic study of imaging mode dependent signal change or BOLD sensitivity as well as the signal changes due tothe tilting angle of the imaging planes. Study has been made for both TRFGE sequence and CGE sequence to compare the distinctions of the each mode since each technique has different sensitivity againsst susceptibility effect. Method of computation and both the computer simulations and their corresponding experimental results are presented.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.6A
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• pp.627-633
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• 2006

In spite of many advantages of Meteor Burst Communications(MBC) on its transmission channel, the fact that its duty rate is less than 10 percents is a considerable deficiency of MBC. To overcome with this deficiency without paying large cost, we use a direct sequence(DS) simple reception system. This method doesn't need to add the components for acquisition or tracking the signal so that it keeps the cost efficiency of the MBC. But it has a disadvantage that its duty rate shows a large decline by noise. For this problem we adopt the proposed system applying a transversal filter circuit. The improvement by this method is more significant when the received signal is weak Simulation results show as t1 is 100ms, then the burst length would be increased by 35% when the initial value of the received signal power increases by 3 dB, t2 is 135ms. If the power increases by 6dB the burst length would be 170ms thus increased by 70%, which shows a great enhancement. Suppose now that t1 is 50ms and the power is increased by 3dB, then t2 would be increased by 70% to 85ms, and increasing the power by 6dB it would be increased by 130% to 115ms.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.38A no.4
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• pp.325-336
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• 2013

FlashLinQ, one of the typical D2D communication systems developed by Qualcomm, considers a single-tone communication based distributed channel-aware link scheduling method to realize the link scheduling process with low control overheads. However, considering the frequency selective fading effect of practical multi-path channel, the single-tone based SIR estimation causes a critical scheduling error problem because the received single-tone signal has quite different channel gain at each sub-carrier location. In order to overcome this problem, we propose a novel link scheduling method based on CAZAC (Constant Amplitude Zero Auto-Correlation) sequence for D2D communication system. In the proposed method, each link has a unique offset value set for the generation of CAZAC sequences. CAZAC sequences with the cyclic offsets are transmitted using multiple sub-blocks in the entire bandwidth, and then each device can obtain nearly full-band SIR using a good cyclic cross-correlation property of CAZAC sequence.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.22 no.8
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• pp.45-51
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• 2008

HID(High Intensity Discharge) search lamp for shipment requires a high open circuit and output current compare than vehicle. This paper presents a soft-start open circuit voltage and constant current sequence control method for 2.5[kW] HID search lamp. The proposed method controls the opal circuit voltage and discharge current of HID lamp according to ignition signal with a simple 8-bit micro-processor and PWM device. For the stable control of lamp, micro-processor checks the output voltage and current. And the checked signals are compared with ignition signal and changes the control mode for stable operation. An ignition signal and micro-processor change the control mode from open circuit voltage contort to constant current control. The proposed control scheme is verified from experimental tests of 2.5[kW] HID search lamp for shipment.

• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.7
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• pp.10-14
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• 2015

Recently, the collection of the sensor data and its analysis become important as the smart buildings equipped with the various sensors appear as a usual scene. The interconnection through the wire cable among the sensors is indispensible because of the information collections such as the temperature, the humidity, and the luminance in the rooms and the hallways for the effective management of the in-building energies. However, these interconnections through the cabling will be very costly, time-consuming, and a difficult task since they will cause some damages to the buildings. Therefore, the interconnections through the unwired connections are required in terms of the deployment effectiveness such as time and cost In this paper, the design and the short sequence operation appropriateness are confirmed through the simulation of the signal measurement system for in-building propagation characteristics based on short signature sequence and the analysis of the system characteristics based on the false alarm probability is performed thereafter.

• Investigative Magnetic Resonance Imaging
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• v.6 no.1
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• pp.21-27
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• 2002

Purpose : To evaluate diffusion-weighted imaging findings of intracerebral hematoma according to the time sequence. Materials and methods : Seventeen patients with intracerebral hematoma were studied. Diffusion weighted images using 1.5 tesla MRI machine were obtained with b-value of $1000{\;}sec/\textrm{mm}^2$. The patients were grouped as hyperacute stage(within 12 hours, 5 patients), acute stage(within 3 days, 4 patients), subacute stage(within 3 weeks, 4 patients), and chronic stage(after 3 weeks,4 patients). The signal intensities were analysed as bright, high, iso, low and dark at the central and peripheral portions of the hematoma in each stage, and compared with those of T2 and T1 weighted images. Results : The signal intensities of the central and peripheral portion of the intracerebral hematoma on diffusion-weighted images were high and dark in hyperacute stage, dark and high-bright in acute stage, and high-bright and dark in subacute and chronic stages. The patterns of signal change of hematoma on diffusion-weighted image according to the time sequence were similar to those on T2-weighted image, but changed early and prominently. Conclusion : The intracerebral hematoma on diffusion-weighted image showed unique central and peripheral signal intensity according to the time sequence. Central portions show high to bright signals in hyperacute, subacute and chronic stage, and dark signal in acute stage, and peripheral portions show dark signals in hyperacute, subacute and chronic stage, and high to bright signal in acute stage.