• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.3
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• pp.496-502
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• 2006

The complexity of IC has rapidly increased as VLSI fabrication technology has grown up quickly. This paper proposes verification methods for data conflicts and protocol between IPs for SoC with coverage metrics. The high-level events is defined to cooperation between blocks or process statement in HDL, or a sequence of performing a job compared to low-level event. They are classified into two categories, resource conflicts and protocol or specification-dependent conflicts. And two coverage metrics used for code coverage in low-level event are proposed to verify the hish-level events. The events of resource conflicts can be detected by using statement coverage metric if global signal or variable has flags in a testbench program, and protocol-dependent events can be checked by data flow metric or path metric.

• Journal of Broadcast Engineering
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• v.17 no.4
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• pp.676-683
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• 2012

Motion estimation in video coding greatly affects implementation complexity. In this paper, a reducing method of the complexity in motion estimation is proposed by using both the depth and color cameras. We obtain object information with video sequence from distance information calculated by depth camera, then perform labeling for grouping pixels within similar distances as the same object. Three search regions (background, inside-object, boundary) are determined adaptively for each of motion estimation blocks within current and reference pictures. If a current block is the inside-object region, then motion is searched within the inside-object region of reference picture. Also if a current block is the background region, then motion is searched within the background region of reference picture. From simulation results, we can see that the proposed method compared to the full search method remains the almost same as the motion estimated difference signal and significantly reduces the searching complexity.

• The KIPS Transactions:PartC
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• v.11C no.3
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• pp.301-308
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• 2004

Misuse IDS is known to have an acceptable accuracy but suffers from high rates of false alarms. We show a behavior based alarm reduction with a memory-based machine learning technique. Our extended form of IBL, (XIBL) examines SNORT alarm signals if that signal is worthy sending signals to security manager. An experiment shows that there exists an apparent difference between true alarms and false alarms with respect to XIBL behavior This gives clear evidence that although an attack in the network consists of a sequence of packets, decisions over Individual packet can be used in conjunction with misuse IDS for better performance.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.5
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• pp.442-448
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• 2006

Human lactoferrin (hLf) is an iron-binding glycoprotein that has been considered to play many biological roles in the human, including the stimulation of the immune system, antimicrobial and anti-inflammatory effects, and regulation of iron absorption. We generated transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing a functional hLf protein using the signal peptide sequence from the endoplasmic reticulum and driven by an oxidative stress-inducible SWPA2 promoter which is highly expressed in plant cell cultures. The production of hLf increased proportionally to cell growth and showed a maximal level (up to 3.6% of total soluble protein) at the stationary phase in suspension cultures. Full-length hLf protein was identified by immunoblot analysis in transgenic cell cultures of Siberian ginseng. Recombinant hLf (rhLf) was purified from suspension cells of Siberian ginseng by ammonium sulfate precipitation, cation-exchange and gel filtration chromatography. N-terminal sequences of rhLf were identical to native hLf (nhLf). The overall monosaccharide composition of rhLf showed the presence of plant specific xylose while sialic acid is absent. Antibacterial activity of purified rhLf was higher than that of nhLf. Taken together, we anticipate that medicinal Siberian ginseng cultured cells, as demonstrated by this study, will be a biotechnologically useful source for commercial production of functional hLf not requiring further purification.

• KSBB Journal
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• v.31 no.2
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• pp.105-112
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• 2016

Ferritin is known to regulate iron metabolism and maintain iron in a variety of the eukaryotic organisms. The region encoding the mature ferritin (0.47 kb, H-type) of Periserrula leucophryna was amplified using the designed primers including restriction enzyme site and termination codon and subcloned in frame to the pRBAS secretion vector containing the signal sequence, RBS, and promoter of amylase gene (E. coli-Bacillus shuttle vector), resulting in recombinant pRBAS-PLF vector. Recombinant ferritin (18 kDa) was correctly processed and secreted from Bacillus subtilis LKS strain harboring the pRBAS-PLF vector and quantitatively analyzed by SDS-PAGE and western blot, respectively. Secretion of the ferritin was optimized by culture conditions (host, medium, temperature, nitrogen source) in 3 L batch culture and 5 L jar fermenter. Finally. the ferritin was largely produced using 50 L fermenter as the following conditions; at $30^{\circ}C$, 150 rpm, 1 vvm in Bacillus subtilis LKS using PY medium. The secreted ferritin was maximally measured (approximately 177.6 ug/ml) when the cell density reached to 14.4 at $OD_{600}$ (20 h incubation). The iron binding activity was confirmed by Perls' staining in 7.5% non-denaturing gel, indicating that the multimeric ferritin (composed of 24 subunits) was formed in the culture broth after secretion. Biologically, the culture broth and powder type containing ferritin were tested for possibility as feed additive in chicken broiler. As a result, the ferritin stimulated the growth of chick broil and improved feed efficiency and production index.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.2C
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• pp.238-245
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• 2010

For orthogonal frequency division multiplexing (OFDM) systems sharing the spectrum with narrow band primary devices, a fractional bandwidth (FBW) mode has been proposed recently to reduce the interference to the primary users. The FBW mode divides the total OFDM bandwidth into subbands and activates (or deactivates) a subset of the subbands according to the result of spectrum sensing. In this paper, we analyze the detection error probability of FBW mode information which is delivered by the sequence embedded in the preamble and evaluate the performance in wireless regional area network environments. The results show that the detection probability derived analytically estimates the actual value from simulation adequately and that a low detection error probability less than $10^{-3}$ is obtained at a low signal-to-noise power ratio.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1938-1944
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• 2008

The procpb gene encoding human procarboxypeptidase B (proCPB, GeneBank access code AJ224866) was cloned and its Pichia expression plasmid, $pPIC9{\alpha}$/hproCPB (9.2 kb), was constructed, in which procpb was under the control of the AOXl promoter and connected to the downstream of the mating factor ${\alpha}$-1 ($MF{\alpha}1$) signal sequence. The plasmid was linearized by digestion with Sacl, and integrated into the genome of P. pastoris strain GS115. By culturing of Pichia transformant on methanol medium, the human proCPB was successfully expressed and secreted into the culture supernatant. Moreover, Western blot analysis of the extracellular proteins showed proCPB bands clearly at a molecular mass of 45 kDa, confirming the expression of proCPB with its right size. The CPB activity reached about 3.5 U/ml and 12.7 U/ml in the flask and fermentor batch cultures of Pichia transformant, respectively. No CPB enzyme activity was found in the intracellular fraction. When the fed-batch cultivation was performed with methanol and glycerol mixture as a feeding medium, the extracellular CPB activity was increased to 42.0 U/ml, which corresponds to a 3.3-fold higher level of CPB activity than that of batch culture. The $K_m$ and $k_{cat}$ values of recombinant human CPB enzyme for hippuryl-$_L$-Arg as a substrate were estimated to be 0.16 mM and $11.93\;sec^{-1}$, respectively.

• Journal of Microbiology and Biotechnology
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• v.19 no.11
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• pp.1306-1318
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• 2009

The filamentous ascomycete Sclerotinia sclerotiorum is well known for its ability to produce a large variety of hydrolytic enzymes. Two $\alpha$-amylases ScAmy54 and ScAmy43 predicted to play an important role in starch degradation were showed to produce specific oligosaccharides essentially maltotriose that have a considerable commercial interest. Primary structure of the two enzymes was established by N-terminal sequencing, MALDI-TOF masse spectrometry and cDNA cloning. The two proteins have the same N-terminal catalytic domain and ScAmy43 derived from ScAmy54 by truncation of 96 amino acids at the carboxyl-terminal region. Data of genomic analysis suggested that the two enzymes originated from the same $\alpha$-amylase gene and that truncation of ScAmy54 to ScAmy43 occurred probably during S. sclerotiorum cultivation. The structural gene of Scamy54 consisted of 9 exons and 8 introns, containing a single 1,500-bp open reading frame encoding 499 amino acids including a signal peptide of 21 residues. ScAmy54 exhibited high amino acid homology with other liquefying fungal $\alpha$-amylases essentially in the four conserved regions and in the putative catalytic triad. A 3D structure model of ScAmy54 and ScAmy43 was built using the 3-D structure of 2guy from A. niger as template. ScAmy54 is composed by three domains A, B, and C, including the well-known $(\beta/\alpha)_8$ barrel motif in domain A, have a typical structure of $\alpha$-amylase family, whereas ScAmy43 contained only tow domains A and B is the first fungal $\alpha$-amylase described until now with the smallest catalytic domain.

• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.167-176
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• 2005

PIGK(phosphatidylinositol glycan, class K) is a subunit of GPI transamidase that cleaves the signal peptide in proproteins and replaces it with GPI. In addition, the structure and synthesis of GPI are critically involved in some of the cellular actions of insulin. Therefore, PIGK would be essential for mammalian development and many specific cellular functions as well as for metabolic activity of insulin associated with GPI. Two types of" full-length cDNAs of porcine PIGK were cloned through RT-PCR and RACE experiments. One is thought to be a normal form(consist of 395 amino acids) and the other is considered as an alternative spliced form(consist of 371 amino acids) which contains additional 63 bps in intron 7. Since a stop codon was contained within the insertion, the spliced form has a shorter coding sequence than that of normal form. A missense mutation (T314I) in exon 6 was detected and used for genotyping to estimate association with the growth and fat deposition traits for 545 $F_2$ animals(Korean native boars ${\times}$ Landrace). From the PCR-RFLP analysis using HpyCH4III, CT genotype showed highly significant relationship(P< 0.01) with carcass fat contents.

• Journal of Satellite, Information and Communications
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• v.8 no.1
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• pp.8-13
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• 2013

It is necessary to develop the next generation FTS which is suitable for our environment and effectively operates many launch vehicles. Standard tone, Secure tone, MHA, EFTS and DSSS are studied for the next generation FTS. FTS requires a high quality of performance and reliability because of their specific mission. And few FTSs are needed but the price is very expensive. Therefore we must investigate a part of the FTS whether the part can be reused for a part of the next FTS. In this paper, we use CCDF of the transmitted signal from FTS as the method to study a possibility of reusing HPA used in the present system. The simulation results show that PEP of Standard tone is 0.21dB and Secure tone and MHA has the same PEP. CPFSK's PEP is 1.81dB and PEP of DSSS using BPSK modulation is 2.6dB.