Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Characterization of Phosphatidylinositol Glycan, Class K (PIGK) Gene and Analysis of Association with Quantitative Traits in Pigs

돼지 Phosphatidylinositol Glycan, Class K (PIGK) 유전자의 동정과 양적형질과의 연관성 분석

  • Lim, H.T. (Division of Applied Life Science, Gyeongsang National University) ;
  • Kim, J.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Choi, B.H. (National Livestock Research Institute, R.D.A.) ;
  • Lee, S.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Park, E.W. (National Livestock Research Institute, R.D.A.) ;
  • Kim, T.H. (National Livestock Research Institute, R.D.A.) ;
  • Cho, I.C. (National Institute of Subtropical Agriculture, R.D.A.) ;
  • Oh, S.J. (National Livestock Research Institute, R.D.A.) ;
  • Lee, J.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University)
  • 임현태 (경상대학교 응용생명과학부) ;
  • 김재환 (경상대학교 응용생명과학부) ;
  • 최봉환 (농촌진흥청 축산연구소) ;
  • 이상호 (경상대학교 응용생명과학부) ;
  • 박응우 (농촌진흥청 축산연구소) ;
  • 김태헌 (농촌진흥청 축산연구소) ;
  • 조인철 (농촌진흥청 난지농업연구소) ;
  • 오성종 (농촌진흥청 축산연구소) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부)
  • Published : 2005.04.30
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Abstract

PIGK(phosphatidylinositol glycan, class K) is a subunit of GPI transamidase that cleaves the signal peptide in proproteins and replaces it with GPI. In addition, the structure and synthesis of GPI are critically involved in some of the cellular actions of insulin. Therefore, PIGK would be essential for mammalian development and many specific cellular functions as well as for metabolic activity of insulin associated with GPI. Two types of" full-length cDNAs of porcine PIGK were cloned through RT-PCR and RACE experiments. One is thought to be a normal form(consist of 395 amino acids) and the other is considered as an alternative spliced form(consist of 371 amino acids) which contains additional 63 bps in intron 7. Since a stop codon was contained within the insertion, the spliced form has a shorter coding sequence than that of normal form. A missense mutation (T314I) in exon 6 was detected and used for genotyping to estimate association with the growth and fat deposition traits for 545 $F_2$ animals(Korean native boars ${\times}$ Landrace). From the PCR-RFLP analysis using HpyCH4III, CT genotype showed highly significant relationship(P< 0.01) with carcass fat contents.

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References

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