• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.11a
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• pp.19-25
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• 1997

Bicyclomycin(BCM)은 1972년에 일본 Hokkaido, Sapporo지방의 토양균중의 하나인 Streptomyces Sapporoneosis의 배양액으로부터 최초로 분리된 항생물질로서 특히 Gram(-) 박테리아인 Escherichia coli. Klebsiella, Shigella, Salmonella, Citrobacter, Enterobacter cloacae, Neisseria gonorrhoeae등에 대한 선택적인 항균효과를 가지고 있고 Streptomycin, Kanamycin, Chloramphenicol, Tetracyclin, Aminobenzylpenicillin 및 naldixic acid 등에 대해 교차내성을 가지고 있지 않다. BCM은 구조적인 면에서 [4.2.2]bicyclic구조를 가지고있는 특이한 항생물질로서 C(1)-triol부분, diketopiperazine부분 그리고 C(5)-C(5a)exomethylene의 3 부분으로 크게 나눌수 있다(Fig. 1). BGM은 E. coli에 대한 항균력으로서 250-500 $\mu\textrm{g}$/mL의 MIC value를 가지고 있고 급성독성으로서 LD$_{50}$가 4g/kg 이상으로 거의 독성이 없는 것이 특징이다. 현재 Fujisawa pharmaceutical Ltd.로부터 Bicozamycin이라는 상품명으로 설사치료제 용도로 시판되고 있다.

• Journal of Microbiology
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• v.40 no.4
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• pp.327-330
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• 2002

The purpose of this research was to test multiplex polymerase chain reaction in investigating the microbiological quality of the risky surfaces in social living places of a military base where over 15 thousand people live together. In 22 samples of 99, there were no bacteria. Only four of the samples contained Shigella, and one of them contained Salmonella, but 77 of the samples contained thermotolerant coliform organisms. There was no statistically significant difference among the microbiological quality of different sites and different equipment surfaces (p>0.05).

• Journal of Environmental Health Sciences
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• v.23 no.1
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• pp.62-65
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• 1997

The insecticidal and antibacterial activity of new synthesized carbamate derivatives(5,7-dibromo-8-hydroxyquinolinyl-N-methylcarbamate(I), 5,7-dibromo-8-hydroxyquinolinyl-N-ethylcarbamate(II)) was examined using 0.2w/v% acetone solutions and 50 $\mu$g/ml-1000 $\mu$g/ml N,N'-dimethylformamide H$_2$O(2:3) solutions of each compounds, respectively. 1. Two carbamates exerted insectiddal effects on Sogata furcifera HORVATH, Delphacodes Striatella FAUEN and Nilaparvate lugens STAL, Whereas no significant effects were observed on the Inazuma dorasalis MOISCHIULSKY Nephateffix apicalis Cincticeps UHLER. 2. These compounds exhibited growth-inhibitory activity against Staphyloccus aureus, Salmonella paratyphi A, Shigella dysenteriae 1a, Escherichia coli NL 1401, at the concentration range of 100-500 $\mu$g/ml in general.

• The Journal of the Korean Society for Microbiology
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• v.17 no.1
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• pp.49-55
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• 1982

Campylobacter fetus subsp. jejuni was isolated from 7 stool specimens(4%) from the total number of 175 stool specimens during February and March, 1982 and the 15 specimens(39.5%) from 33 stool specimens of the chicken in the urban area of Seoul. All isolates showed typical characteristics of the Campylobacter fetus subsp. jejuni in Gram reaction, morphology and biochemical tests. They were all resistant to cephazolin and sensitive to gentamicin and chloramphenicol. During same period of time, the shigella was isolated from 11 stool specimens and the salmonella was isolated from 1 stool specimen.

• Journal of Microbiology and Biotechnology
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• v.4 no.4
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• pp.250-255
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• 1994

Wild-type and four mutant R100 merR genes were cloned and the proteins overproduced under tac promoter control of pKK223-3. His118Ala, Cys117Ser, Cys126Ser, and wild-type MerR were successfully overproduced although amino-terminal 14 amino acids deletion mutant MerR was not successful. The amount of overproduced wild-type MerR protein as well as other mutant MerR was between 15%-20% of the total protein. The protein was able to be purified up to 95% homogeneity. Specific DNA-protein blotting experiments showed that the 95 bp operator containing DNA fragment could bind to Cys126Ser, His118Ala, and wild- type MerR, but not to Cys117Ser. These results were consistent with the previously reported complementation experiment results that His118Ala, Cys126Ser, and wild-type MerR could repress the mer operon but Cys117Ser could not.

• Microbiology and Biotechnology Letters
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• v.31 no.1
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• pp.32-35
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• 2003

This study was carried out to determine the inhibitory effect of garlic juice against Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Shigella flexneri. Staphylococcus aureus, Streptococcus mutans, Virio. parahaemolyticus which are food pathogenic bacteria and Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus casei, Lactobacillus plantarum, Lactococcus. lactis, Leuconostoc mesenteroides which are lactic acid bacteria. An aqueous extract of garlic was bacteriocidal against Gram-positive and Gram-negative bacteria in all concentrations (0.1∼2.5(w/v)%) tested in this experiment. Especially 0.5(w/v)% garlic juice inactivated completely E. coli, S. typhimurium, S. flexineri, V. parahaemolyticus and 1.0(w/v)% garlic juice perfectly reduced P. aeruginosa, S. mutans. Generally, the experiment result indicate that garlic juice restrains the growth of the pathogenic bacteria better than the lactic acid bacteria. Therefore, garlic has potential for the preservation of processed foods.

• Clinical and Experimental Pediatrics
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• v.54 no.7
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• pp.313-315
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• 2011

Reactive arthritis comprises a subgroup within infection-associated arthritides in genetically susceptible hosts. Researchers and clinicians recognize two clinical forms of reactive arthritis which occurs after genitourinary tract infection and after gastrointestinal tract infection. Chlamydia infection has been implicated as the most common agent associated with post-venereal reactive arthritis. Studies have proposed Shigella infection, Salmonella infection, or Yersinia infection as the microorganisms responsible for the post-dysenteric form. The human leukocyte antigen (HLA)-B27 antigen is the best-known predisposing factor. We report a case of HLA-B27-associated reactive arthritis after Salmonella enteritis at Pusan National University hospital.

• Journal of environmental and Sanitary engineering
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• v.18 no.1
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• pp.1-7
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• 2003

There are lots of waterborne diseases which are caused by pathogenic microorganisms disseminated in contaminated environment. The purpose of this study is to investigate the status of water qualify and in order to investigate the sanitary condition of water purifier in northern part of Gyeonggi-do. We analyzed pathogenic microorganisms and 44 items of drinking water criteria from April to July in 2002. The results were as follows. 1. In 774 samples of water purifier, Pathogenic bacteria(salmonella, E coli O-157, O-26, O-111, shigella, pseudomonas etc.) were not isolated and total coliforms not detected. 2. About 76.5% of the water with purifier are found to be appropriated to the drinking water quality criteria. but 182 samples(23.5%) were without limits of drinking water criteria. 3. In the microbiological examination, 774 samples of purified water showed that 169 samples(21.9%) exceed the range of recommended limits(100 $CFU/m{\ell}$) to the total viable bacterial counts by pour plate method.

• Genomics & Informatics
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• v.1 no.1
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• pp.55-60
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• 2003

The nucleotide sequence of pZMO1, a small cryptic plasmid of Zymomonas mobilis ATCC10988 was determined. Analysis of 1,680 bp of sequence revealed $69\%$ identity with Shigella sonnei plasmid, pKYM and $61\%$ identity with Nostoc sp. ss DNA replicating plasmid. Analysis of a deduced amino acid sequence of an orf of pZMO1 revealed $75\%$ identity and $90\%$ similarity with the repA gene of Synechocystis sp. plasmid pCA2.4. The upstream region of the repA gene of pZMO1 possesses six directed repeat sequences and two inverted repeat sequences at downstream of the IR consensus sequence of nick region of rolling circle replication (RCR) plasmid. A typical terminator hairpin structure was found at the downstream region of repA gene. Degradation of single-stranded plasmid DNA by S1 nuclease was detected by Southern hybridization. It suggests that pZMO1 replicates by a rolling circle mechanism in Z. mobilis ATCC10988 cells.

• Journal of Environmental Health Sciences
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• v.21 no.1
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• pp.74-77
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• 1995

The insecticidal and antibacterial activity of new synthesized carbamate derivatives(8-Hydroxy-5-chloroquinolyl-N-methylcarbamate(I), 8-Hydroxy-5-chloroquinolyl-N-ethylcarbamate(II)) was examined using 0.2 w/v% acetone solutiolas and 50 r/ml~1000 r/m N,N'-dimethylformamide-$H_2O$(2:3) solutions of each compounds, respectively. 1) Two carbamates exerted insecticidal effects on Sogata furcifera HORVATH, Delphacodes Striatella FAUEN, Whereas no significant effects were observed on the Nilaparvate lugens STAL, Inazuma dorasails MOISCHIULSKY and Nephateffix apicalis Cincticeps UHLER. 2) These compounds exhibited growth-inhibitory activity against Staphylococcus aureus, Salmonella paratyphi A, Shigella dysenteriae 1a, Escherichia coli NL 1401,at the concentration range of 100~500 r/ml in general.