Determination of Enteric Bacteria at Microbiologically Risky Points by Multiplex Polymerase Chain Reaction

  • Mahir Gulec (Department of Public Health, Gulhane Millitary Medical Academy) ;
  • Bilal Bakir (Department of Public Health, Gulhane Millitary Medical Academy) ;
  • Recai Ogur (Department of Public Health, Gulhane Millitary Medical Academy) ;
  • Tekbas, Omer-Faruk (Department of Public Health, Gulhane Millitary Medical Academy)
  • Published : 2002.12.01

Abstract

The purpose of this research was to test multiplex polymerase chain reaction in investigating the microbiological quality of the risky surfaces in social living places of a military base where over 15 thousand people live together. In 22 samples of 99, there were no bacteria. Only four of the samples contained Shigella, and one of them contained Salmonella, but 77 of the samples contained thermotolerant coliform organisms. There was no statistically significant difference among the microbiological quality of different sites and different equipment surfaces (p>0.05).

Keywords

References

  1. Appl Environ Microbiol. v.57 Detection of coliform bacteria and Escherichia coli by multiplex polymease chain reaction: comparison with defined subrtrate and plating methods for water quality monitiring. Bej,A.K.;S.C.McCarty;R.M.Atlas.
  2. Appl Environ Microbiol. v.56 Detection of coliform bacteria in water by polymerase chain reaction and gene probes. Bej,A.K.R.J.Steffan;J.DiCesare;L.Haff.
  3. Zentralbl. Bakteriol. Mikrobiol.Hyg. v.183 Hygiene problems in dental practice with special attention to dental units. Borneff,M.
  4. Public Health & Preventive Medicine. 14th ed. Water Quality Management. Conway,J.B.;Wallace R.B. Ed.
  5. Appl Environ Mirocbiol. v.46 Survival and detection of rotaviruses on environmental surfaces in day care centers. Keswick,B.H.;L.K.Pickering;H.L.DuPont.
  6. J. Dairy Sci. v.73 Efficiency of sanitizing agents for destroying Listeria monocytogenes on contaminated surfaces. Mafu,A.A.;D.Boy;J.Goulet.
  7. Lab Delo. v.11 Comparative evaluation of methods for tne study of surface contamination with bacteria of the genus Escherichia coli. Natsiashvili,E.
  8. Zentralbl.Bakteriol. v.167 Hygienic and bacteriological comparative studies in 50 hospitals. V.Bacterial contamination of hospital surfaces. Pfeiffer,E.H.;J.R.Wittig;H.Dunkelberg.
  9. Detection and Identification of Enteric Pathogens. no.5627275 Roll,B.
  10. Mirovbiol Rev. v.51 Survival strategies in the natural environment. Roszack,D.B.;R.R.Colwell.
  11. Z Gesamte Hyg. v.17 Culture media plates for quantitative and qualitative determination of the level of surface contamination. Schreiber,M.
  12. J. Appt.Bacteriol. v.56 A comparison of contact plate and calcium alginate swab techniques for quantitative assessment of bacteriological contamination of environmental surfaces. Scott,E.;S.F.Bloomfield;C.G.Barlow.
  13. Br. Vet. J. v.129 A reproducible surface contamination method for disinfactant tests. Slavin,G.
  14. Zentralbl. Bakteriol. v.161 Test for the efficacy of disinfectants at surfaces in test models. I.(communication). Dependence of experimental results on the method of demonstration of surviving germs. Spicher,G.;J.Peters.