[KSCI] Korea Science Citation Index Service

Determination of Enteric Bacteria at Microbiologically Risky Points by Multiplex Polymerase Chain Reaction

Mahir Gulec (Department of Public Health, Gulhane Millitary Medical Academy)
Bilal Bakir (Department of Public Health, Gulhane Millitary Medical Academy)
Recai Ogur (Department of Public Health, Gulhane Millitary Medical Academy)
Tekbas, Omer-Faruk (Department of Public Health, Gulhane Millitary Medical Academy)

Publication Information

Journal of Microbiology / v.40, no.4, 2002 , pp. 327-330 More about this Journal

Abstract

The purpose of this research was to test multiplex polymerase chain reaction in investigating the microbiological quality of the risky surfaces in social living places of a military base where over 15 thousand people live together. In 22 samples of 99, there were no bacteria. Only four of the samples contained Shigella, and one of them contained Salmonella, but 77 of the samples contained thermotolerant coliform organisms. There was no statistically significant difference among the microbiological quality of different sites and different equipment surfaces (p>0.05).

Keywords

swab; coliform organisms; multiplex polymerase chain reaction; surface contamination;

Citations & Related Records

Times Cited By Web Of Science : 0 (Related Records In Web of Science)
Times Cited By SCOPUS : 0

Reference

1	Test for the efficacy of disinfectants at surfaces in test models. I.(communication). Dependence of experimental results on the method of demonstration of surviving germs. / [ Spicher,G.;J.Peters. ] / Zentralbl. Bakteriol.
2	A comparison of contact plate and calcium alginate swab techniques for quantitative assessment of bacteriological contamination of environmental surfaces. / [ Scott,E.;S.F.Bloomfield;C.G.Barlow. ] / J. Appt.Bacteriol.
3	Hygiene problems in dental practice with special attention to dental units. / [ Borneff,M. ] / Zentralbl. Bakteriol. Mikrobiol.Hyg.
4	Efficiency of sanitizing agents for destroying Listeria monocytogenes on contaminated surfaces. / [ Mafu,A.A.;D.Boy;J.Goulet. ] / J. Dairy Sci. ScienceOn
5	Survival and detection of rotaviruses on environmental surfaces in day care centers. / [ Keswick,B.H.;L.K.Pickering;H.L.DuPont. ] / Appl Environ Mirocbiol.
6	Detection of coliform bacteria and Escherichia coli by multiplex polymease chain reaction: comparison with defined subrtrate and plating methods for water quality monitiring. / [ Bej,A.K.;S.C.McCarty;R.M.Atlas. ] / Appl Environ Microbiol.
7	Survival strategies in the natural environment. / [ Roszack,D.B.;R.R.Colwell. ] / Mirovbiol Rev.
8	Detection of coliform bacteria in water by polymerase chain reaction and gene probes. / [ Bej,A.K.R.J.Steffan;J.DiCesare;L.Haff. ] / Appl Environ Microbiol.
9	Culture media plates for quantitative and qualitative determination of the level of surface contamination. / [ Schreiber,M. ] / Z Gesamte Hyg.
10	Comparative evaluation of methods for tne study of surface contamination with bacteria of the genus Escherichia coli. / [ Natsiashvili,E. ] / Lab Delo.
11	Hygienic and bacteriological comparative studies in 50 hospitals. V.Bacterial contamination of hospital surfaces. / [ Pfeiffer,E.H.;J.R.Wittig;H.Dunkelberg. ] / Zentralbl.Bakteriol.
12	Water Quality Management. / [ Conway,J.B.;Wallace R.B. Ed. ] / Public Health & Preventive Medicine. 14th ed.
13	A reproducible surface contamination method for disinfactant tests. / [ Slavin,G. ] / Br. Vet. J.
14	/ [ Roll,B. ] / Detection and Identification of Enteric Pathogens.