• 산업기술연구
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• 제8권
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• pp.13-21
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• 1988

For the improvement of productivity of Pleurotus ostreatus, the production of liquid spawn was studied. The highest liquid spawn production was obtained after shaking culture for 4 days in the culture medium containing 5%(W/V) wheat flour, 0.2%(W/V) yeast extract, 0.1%(W/V)$KNO_3$ 0.05% (W/V) $MgSO_4{\cdot}7H_2O$, 0.05%(W/V) $KH_2PO_4$. The optimum pH and temperature was 7.0 ana $30^{\circ}C$. The period required to complete the mycelial growth after spawning were 28, 22, 10 and 9 days, respectively, when the 2%(V/V) of solid spawn and 2%(V/V), 5% (V/V) and 10%(V/V) of liquid spawn were inoculated. The days required from spawning to fruiting bodies were 38, 34, 28 and 27 days.

• 화훼연구
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• 제17권2호
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• pp.93-100
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• 2009

본 연구는 청나래고사리의 기내 포자체 유묘를 이용한 효율적인 대량생산 방법을 개발하기 위하여 시행되었다. 유묘의 엽신, 엽병, 근경을 곱게 다져 활성탄 0.1%를 첨가한 1/2MS배지에 배양한 결과, 근경의 절편에서만 포자체 유묘가 생산되었다. 곱게 다진 근경 절편은 1/2MS 배지에서 포자체 재생이 가장 왕성하였으며, sucrose 농도를 2%로 조절하고 $NaH_2PO_4$ $50mgL^{-1}$을 첨가할 경우 포자체 재생이 더욱 촉진되었다. Kinetin과 BA를 단용 또는 NAA, IBA와 각각 혼용하여 배양한 결과, kinetin $1{\mu}M$ 단용 처리구에서 포자체 재생이 가장 왕성하였다. BA 첨가구에서는 분열조직의 증식이 왕성하였으나. 분열조직이 포자체로 분화되지 못하는 특징을 보였다. 변형 1/2MS 배지(sucrose 2%, $NaH_2PO_4$ $50mgL^{-1}$, kinetin $1{\mu}M$, pH 5.8, agar 0.8%)에 활성탄 0 또는 0.1%를 첨가하여 고체, 액체 정체, 액체 진탕배양한 결과, 포자체 재생은 액체 진탕배양시 가장 왕성하였으며, 활성탄의 첨가는 포자체 재생을 촉진하였다.

• 미생물학회지
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• 제40권2호
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• pp.160-166
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• 2004

The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$PO$_4$, 0.05% The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % KH$_2$The optimal conditions for production of Monascus sp. KM100l cell mass on submerged culture and production of monacolin K on rice solid culture were investigated. An overproducing mutant of Monascus pigments, KM 1001 mutant, from Monascus purpureus KCCM60016 was selected by NTG treatment. The optimal medium for the production of KM100l mutant cell mass is instructed to be composed of 3% glucose, 2% yeast extract, 0.1 % $(KH_2PO_4$, 0.05% $MgSO_4{\cdot}7H_2O$, 0.2% L-asparagine, pH 4.5, and the optimal inoculum size and shaking speed were $1.5{\times}10^6$ spores/50 m1 medium and 150 rpm, respectively. On optimal conditions, 4.1 g/l of the cell mass was obtained at 28$^{\circ}C$ for 3 days. The mycelium were inoculated on 500 g of steamed rice using vinyl bag ($30.6{\times}44$ cm) and incubated at $30^{\circ}C$, 85% humidity for 21 days. Lactone form monacolin K was rapidly increased for 2 days and reached highest concentration of monacolin K (2,930 mg/kg) for 15 days, and monacolin K was decreased after 15 days.

• 한국환경과학회지
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• 제12권10호
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• pp.1079-1084
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• 2003

This study was carried out to check changes of components in the slaughter waste by the bacteria isolated from slaughter wastes from Gyungnam Province from May to June 2000, and to find usefu] organism for treatment of the waste. Bacteria used in this study were Aeromonas hydrophila, as the dominant of the waste. Optimum conditions for bacterial culture were obtained as the temperature of 35$^{\circ}C$, pH 6.5, and shaking of 120 rpm in nutrient broth. The mean values of dissolved oxygen was 4.14 mg/1; biochemical oxygen demand, 1731.21 mg/1; ratio of BOD/COD, 0.53-0.64; ratio of T-P/T-N, 1.0-1.41; and viable counts of the waste, 5.47${\times}$10$\^$7/ CFU. Little change in total nitrogen observed by 36 hr of the culture. The largest amount of increasing NH$_4$$\^$+/-N was observed in the sample that 10% of the waste added in nutrient broth with A. hydrophila showing the value of 29.19 mg/l at the beginning to 570.36 mg/1 by 36 hr of culture. However, the highest increasing ratio between initial amount and finals at 36 hr of culture showed as 41.6 times when 3% of the waste added. NO$_3$ -N was decreased showing the value of 71.27 mg/1 to 32.14 mg/1 by 24 hr of culture with the organism when 10% of the waste added in nutrient broth. Total phosphorus was decreased showing the value from 188.74 mg/1 to 101.41 mg/1 after 12 hr of culture with the organism when 5% of the waste added in nutrient broth, while T-P was decreased gradually by 24 hr of culture from 193.8 to 101.4 mg/1 when 10% of the waste added.

• KSBB Journal
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• 제19권6호
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• pp.451-456
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• 2004

교반배양시 배지에 첨가되는 에탄올의 농도가 증가할수록 $Cel^-$ mutant의 발생율이 증가하므로 $1.5\%$ 에탄올이 첨가된 배지의 경우 500 rpm보다 높은 교반속도를 유지해야 효과적으로 $Cel^-$ mutant의 발생이 억제되고 BC 생산량을 증가시킬 수 있다. 교반 배양과 같은 강한 shear stress의 배양환경 하에서는 진탕배양의 경우와 달리 $1.0\%$ 에탄올을 분할공급하더라도 BC 생산의 효율성을 높일 수 없었다. Phosphate ion이 첨가된 배지보다 첨가되지 않은 배지에서 BC 생산량이 더 높았다. 배지조성 중 아세트산의 농도를 $1.0\%$로 증가시키면 균체의 건조중량은 변화가 없었으나 BC 생산량이 감소되었다. 배양액의 pH 제어는 BC의 생산에는 영향을 미치지 않았으나 균체의 성장과 WSPS의 생산을 향상시켰다.

• KSBB Journal
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• 제25권3호
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• pp.283-288
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• 2010

We investigated the feasibility of bioethanol production from hydrolysate of brown seaweed Sargassum sagamianum. Prior to bioethanol production using yeasts, six yeast strains were compared and the best ones in terms of the ethanol production levels were selected. Pichia stipitis ATCC 7126, Pichia stipitis ATCC 58784, and Pichia stipitis ATCC 58376 were superior to others in terms of ethanol production. These yeast strains were used for producing bioethanol by the shaking bottle culture and the fermentor culture. Out of approximately 30 g/L reducing sugar, about 3~6 g/L and 4~7 g/L bioethanol were produced in the bottle culture and the fermentor one, respectively. Furthermore, it was observed that around 12~28 g-bioethanol was produced from 1 kilogram of Sargassum sagamianum. Compared with those previously published, these data were almost three to eight times higher in value.

• 한국미생물·생명공학회지
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• 제21권4호
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• pp.329-335
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• 1993

Ascorbate oxidase activity in various cucumber tissue extracts was highest in young fruit peeling. Cucumber callus was induced from young fruit peeling and callus cell lines were selected for more than 7 months, which porduced high levels of ascorbate oxidase and had a high growth rate. Induction of callus was optimized with Linsmaier-Skoog(LS) medium at 25$^{\circ}C$ in dark phase. Ascorbate oxidase activity reached a maximum at 5 days after transfer to LS basal liquid-medium ant then declined. The enzyme activity in callus cells was stimulated by addition of 10${\mu}$M $CuSO_4$ in the early logarithmic phase of growth. And also, adding 10${\mu}$M $CuSO_4$ at 3rd day 7th day of culture period, ascorbate oxidase activity in callus cells was maintained to high level. Maximum yield of ascorbate oxidase was found at the 25th day by flask shaking culture, but three-fold of ascorbate oxidase activity was obtained at the 16th day by jar fermentation.

• Journal of Microbiology and Biotechnology
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• 제30권1호
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• pp.93-100
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• 2020

A bacterial strain inhibiting the growth of Vibrio anguillarum, the causative agent of vibriosis, was isolated from fish intestines. The isolated strain HS36 was identified as Aerococcus urinaeequi based on the characteristics of the genus according to Bergey's Manual of Systematic Bacteriology and by 16S rRNA sequencing. The growth rate and antibacterial activity of strain HS36 in shaking culture were higher than those in static culture, while the optimal pH and temperature for antibacterial activity were 7.0 and 30℃, respectively. The active antibacterial substance was purified from a culture broth of A. urinaeequi HS36 by Sephadex G-75 gel chromatography, Sephadex G-25 gel chromatography, and reverse-phase high-performance liquid chromatography. Its molecular weight, as estimated by Tricine SDS-polyacrylamide gel electrophoresis, was approximately 1,000 Da. The antibacterial substance produced by strain HS36 was stable after incubation for 1 h at 100℃. Although its antibacterial activity was optimal at pH 6-8, activity was retained at a pH range from 2 to 11. The purified antibacterial substance was inactivated by proteinase K, papain, and β-amylase treatment. The newly purified antibacterial substance, classified as a class II bacteriocin, inhibited the growth of Klebsiella pneumoniae, Salmonella enterica, and Vibrio alginolyticus.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.383-388
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• 2004

The conversion of a cellulose-producing cell ($Cel^+$) from Gluconacetobacter hansenii PJK (KCTC 10505 BP) to a non-cellulose-producing cell ($Cel^-$) was investigated by measuring the colony forming unit (CFU). This was achieved in a shaking flask with three slanted baffles, which exerted a strong shear stress. The addition of organic acid, such as glutamic acid and acetic acid, induced the conversion of microbial cells from a wild type to $Cel^-$ mutants in a flask culture. The supplementation of $1\%$ ethanol to the medium containing an organic acid depressed the con-version of the microbial cells to $Cel^-$ mutants in a conventional flask without slanted baffles. The addition of ethanol to the medium containing an organic acid; however, accelerated the conversion of microbial cells in the flask with slanted baffles. The $Cel^+$ cells from the agitated culture were not easily converted into $Cel^-$ mutants on the additions of organic acid and ethanol to a flask without Slanted baffles, but some portion of the $Cel^+$ cells were converted to $Cel^-$ mutants in a flask with slanted baffles. The conversion ratio of $Cel^+$ cells to $Cel^-$ mutants was strongly re-lated to the production of bacterial cellulose independently from the cell growth.

• 유기물자원화
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• 제9권1호
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• pp.49-55
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• 2001

남은 음식물을 갈아 액상으로 만든 기질을 이용하여 생효모를 함유한 생균제제사료(probiotics)를 생산하기 위해 효모의 호기적 액상발효를 시도하였다. 기질의 영양조성의 최적화를 위해 미리 진탕배양한 결과를 토대로 액상의 남은 음식물 기질에 우레아($0.5g/{\ell}$), 당밀($4g/{\ell}$), O-phosphate($0.4g/{\ell}$), yeast extract($1g/{\ell}$)를 첨가하여 2리터 용량의 발효기를 이용하여 Kluyveromyces marxianus와 Aspersillus. oryzae를 접종하여 통기발효 시킨 결과 12시간 내에 $1.4{\times}10^{10}/liter$의 생균효모농도를 얻을 수 있었다.