• 한국식품영양과학회지
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• 제40권12호
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• pp.1708-1714
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• 2011

본 연구는 느티만가닥버섯의 항비만 효과를 알아보기 위해 4주령된 C57BL/6 수컷 쥐를 30마리를 1주일간 적응시킨 후, 정상식이군, 고지방식이군, 5% 느티만가닥버섯 분말 첨가 식이군으로 나누어 8주간 실시하였다. 체중 및 체중증가량은 고지방식이군에서 유의적으로 증가하였으며, 느티만 가닥버섯 분말 첨가 식이군에서 감소하였다. 간 및 지방조직의 무게도 고지방식이군에 비하여 느티만가닥버섯 처리군에서 낮았다. 고지방 식이에 의한 혈청 중 총콜레스테롤, 인슐린 및 렙틴 수준 증가는 느티만가닥버섯 분말 섭취로 인하여 낮아졌다. 또한, 간 중 지질 함량도 느티만가닥버섯 분말 첨가군에서 감소하였다. HE staining으로 관찰한 간내 지방축적과 지방세포의 크기도 느티만가닥버섯 분말 첨가 식이군에서 현저히 감소하였다. 부고환 지방조직에서 관찰한 지방생성 관련 유전자인 $PPAR{\gamma}$, SREBP-1c, SCD-1의 단백질 발현은 고지방식이군과 비교 시, 느티만가닥버섯 분말첨가 식이군에서 억제되었다. 이상의 실험결과, 고지방 식이와 함께 공급한 느티만가닥버섯 분말은 체중 감소 및 체지방 축적 억제와 더불어 혈청 지방 수준 개선에도 긍정적 영향을 미치는 것으로 나타났다.

• 한국식품영양과학회지
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• 제29권6호
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• pp.1090-1097
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• 2000

본 연구는 여대생들을 대상으로 1997년 9월 22일부터 10월 12일까지의 3주간에 거친 식사기록을 통해 긴 기간 동안의 열량, 열량영양소 섭취량 및 열량 영양소 구성비와 신체계측치, 인슐린, 혈청지질과의 관련성을 살펴보고자 실시하였다. 섭취열량 및 열량영양소 섭취량과 가장 빈번하게 상관성을 보인 신체 계측치는 허리둘레였는 데 허리둘레는 평균 열량, 탄수화물, 단백질 섭취량과 각각 유의한 정상관관계를 보였으나 열량 영양소 구성비와는 상관이 없었다. 이에 비해 BMI는 평균탄수화물 구성비와는 상관이 없었다. 이에 반해 BMI는 평균 탄수화물 구성비와 유의한 정상관계를 보였으나, 체중, BMI, 체지방비, 체지방량과 열량 및 열량영양소 섭취량과는 서로 상관이 없었다. 또 인슐린은 평균 섭취열량 및 단백질 섭취량과 각각 정상관관계를 보였지만 3대 열량영양소 구성비와는 상관이 없었다. 혈청 중성지방은 평균 지방 섭취량 및 지방 구성비와 역상관을 보여 지방섭취와 가장 관련이 있는 변인임을 알 수 있었다. 또 HDL-cholesterol은 평균 단백질 섭취비와 정상관계를 보여 단백질 섭취비와 가장 관련이 있는 변이임을 알 수 있었다. 정상체중의 여대생을 대상으로 3주간의 식사기록을 통해 식사 중의 열량, 3대 열량영양소 섭취량 및 그 구성비와 신체적 생화학적 변인과의 관련성을 살펴본 결과, 신체계측치 중 열량 및 열량영양소 섭취량과 가장 빈번하게 상관성을 보인 신체계측치는 허리둘레였고, 생화학적 변인으로는 인슐인이었지만 구 변이 모두 열량영양소 구성비와 가장 관련이 있는 변이는 BMI였고, 혈청 중성지방은 지방 섭취량 및 지방구성비와 상관이 있었으며, HDL-cholesterol은 단백질 섭취비와 관련이 있었다.

• 한국식품영양과학회지
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• 제43권4호
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• pp.485-490
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• 2014

본 연구는 흰쥐를 이용하여 고콜레스테롤 식이로 고콜레스테롤혈증을 유도하고 이에 천년초 추출물을 2, 4% 식이에 포함시켜 경구로 섭취 시의 천년초 추출물의 콜레스레롤 개선 효과를 알아보고자 하였다. 이에 혈중 및 간 조직 내 지질성분, 혈중 간 손상 지표, 분변 내 지질 성분 및 담즙산을 분석하였다. 천년초 추출물 섭취는 고콜레스테롤 식이로 상승된 혈청 총 콜레스테롤과 저밀도 지단백 콜레스테롤을 정상 수준으로 감소시켰으며 고밀도 콜레스테롤은 증가시켜 혈중 콜레스테롤 개선 효과를 나타내었다. 고콜레스테롤 식이 유도로 인해 상승된 혈중 ALT와 AST는 천년초 추출물 처치로 감소되는 경향을 보였으며 고콜레스테롤 식이로 인해 상승된 간 조직 내의 총 콜레스테롤과 중성 지방 역시 천년초 추출물 처치로 인해 용량 의존적으로 감소되었다. 분변 내 중성 지방과 담즙산은 천년초 추출물 처치로 유의적으로 상승되어 천년물 추출물 섭취로 중성 지방과 담즙산 배설이 유도가 이루어짐을 알 수 있었다. 본 연구 결과 천년초 추출물은 고콜레스테롤 식이로 고콜레스테롤이 유도된 쥐에 있어 콜레스테롤 개선 효과의 가능성 있는 식품 소재로 판단되며, 이를 인체에 적용시키기 위해서는 이에 대한 기전연구와 활성 물질 동정에 대한 계속적인 연구가 이루어져야 할 것으로 생각되어진다.

• 한국가금학회지
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• 제37권3호
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• pp.207-213
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• 2010

본 시험은 목초액이 토종닭 한협 3호의 생산성, 유해 가스 발생량, 혈액 성상 그리고 가슴육의 지방산 함량에 미치는 영향을 평가하기 위하여 토종닭 사료에 수준별로 첨가하여 70일 동안 급여하였다. 목초액은 0, 0.1, 0.2% 수준으로 첨가 급여하였고, 처리구당 5반복, 반복당 16수씩 총 240수를 시험에 이용하였다. 시험 결과, 목초액은 생산성에 있어서 큰 영향을 미치지 못하였으나, 암모니아 가스 발생량은 대조구에 비하여 유의하게 감소시켰다(p<0.05). 혈중 total cholesterol과 HDL-cholesterol 농도에 있어서는 목초액 처리구가 대조구에 비하여 유의하게 증가한 반면 triglyceride의 농도는 감소하였다(p<0.05). 가슴육의 포화지방산인 myristic acid와 stearic acid는 목초액을 급여함에 따라 대조구에 비하여 유의적으로 감소하였으나, 불포화지방산인 oleic acid와 linolenic acid는 유의적으로 증가하였다(P<0.05). 따라서 본 연구 결과, 목초액은 유해 가스 저감 효과가 있으며, 토종닭의 혈중 지질 수준을 개선시키고, 가슴육의 포화지방산 함량을 감소시키고 단쇄 불포화지방산의 함량은 증가시키는 것으로 판단된다.

• Journal of Nutrition and Health
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• 제31권3호
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• pp.253-262
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• 1998

This study was performed to investigated the effect of dietary fat sources on renal senescence in aged rats. Seventeen month old male Sprague-Dawley rats were divided into 3 groups according to urinary protein excretion. Four month old rats were used as a control group. The rats were fed one of three different experimental diets ; 20% beef tallow, 20% corn oil 20% fish oil diet. They were fed experimental diets ad libitum for 16 weeks . The results are summarized as follows. Serum lipid concentrations were higher in aged rats than in control rats, with the beef tallow group showing the highest level, followed by the corn oil and fish oil groups. Old rats showed higher HDL and lower LDL levels than the control groups. Age and dietary fat had no effect on VLDL. GFR for the both age groups were increased with experimental period with the beef tallow group showing the highest value. Urinary protein excretion was also increased with experimental period in both age groups. There was a large increase in urinary protein in old rats that were fed beef tallow and corn oil, but not in old rats fed fish oil. On the contrary , the effect of dietary fat on urinary protein was not found in control groups. There was individual susceptibility in the effect of dietary fat on urinary protein. Old rats fed beef tallow with high initial urinary protein showed highest increase, but , the change was not significant in rats with a low initial value . It was also found that the increase was kept low in rats of the fish oil group with high initial urinary protein. The corn oil group showed in between values. There were no differences in urine and renal tissue concentrations of TXB2 . Aged rats showed a tendency of having higher urinary PGE2 excretion and lower renal cortex content. Since higher PGE2 excretion was reported to be associated with decreased renal function, this might suggest that the aged rats show renal function reduction. Light microscopic examination showed that glomerular segmental sclerosis, mesangial matrix expansion and tubular atrophy were more frequently present in aged rats, and that these changes were more significant in the beef tallow group, followed by corn oil and fish oil groups. The percentage of urinary protein excretion was increased in aged rats in association with increased glomerular sclerosis and mesangial matrix . This change could be partly due to a change in eicosanoids metabolism . Therefore, modification of dietary fat could affect the eicosanoids metabolism in kidney and renal senescence.

• 대한본초학회지
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• 제30권4호
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• pp.109-119
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• 2015

Objectives : The aim of this study was to evaluate the effect of aqueous extracts of Mume Fructus(MF) on the 6-n-propyl-2-thiouracil(PTU)-induced rat hypothyroidism.Methods : Aqueous extracts of MF(yield = 19.38%) were administered, once per day for 42 days from 2 weeks before starting of PTU treatment as an oral dose of 300 and 150 ㎎/㎏(body weight), and hypothyroidism was induced by daily subcutaneous treatment of PTU 10 ㎎/㎏ for 28 days. The changes in the body weight, thyroid gland weights, liver weight, serum levels of thyroid hormone-thyroid stimulating hormone(TSH), tri-iodothyronine(T₃) and thyroxine(T₄), total cholesterol, low density lipoprotein(LDL), high density lipoprotein(HDL) and triglyceride (TG), aspartate aminotransferase(AST), alanine aminotransferase(ALT), liver antioxidant defense system-lipid peroxidation, H₂O₂, superoxide dismutase(SOD) and catalase(CAT) were examined with histopathology of thyroid glands and liver.Results : Results were compared with LevoT₄0.5 ㎎/㎏ treated rats. MF extracts recovered from the decreases in the body weight, liver weight, T₃and T₄, TG, liver CAT activities as results of PTU treatment. And MF extracts recovered from the increases of thyroid gland weights, TSH, HDL contents, liver H₂O₂, AST as results of PTU treatment. In addition, these PTU-induced histopathological changes in thyroid glands and liver related to hypothyroidism were dramatically decreased by treatment of both different dosages of MF extract, respectively.Conclusions : According to the above results, it is suggested that MF extracts have advantageous effects on the thyroid hormone productions with beneficial effects on the hypothyroidism related liver injuries mediated by the modulation on the antioxidant system.

• 생명과학회지
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• 제19권3호
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• pp.362-365
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• 2009

Hepatocyte nuclear factor-$4{\alpha}$ (HNF-$4{\alpha}$)는 췌장 베타세포의 발생, 분화에 중요한 역할을 하는 전사인자로서, 그 유전자 변이와 MODY와의 연관성이 보고된 바 있다. 특히, 일본인의 경우에는 HNF-$4{\alpha}$ 12352 C>T가 제2형 당뇨병과의 연관성이 있다고 보고되어 있다. 이에 본 연구에서는 HNF-$4{\alpha}$의 유전자 변이와 제2형 당뇨병 사이에 어떠한 연관성이 한국인에서도 있는지 알아보았다. 그 결과, HNF-$4{\alpha}$의 유전자 변이인 12352 C>T와 제2형 당뇨병사이에는 구체적인 연관성이 없는 것으로 확인되었다. 이는 제2형 당뇨병과의 연관성이 있다고 보고된 이전의 일본인이나 유럽인을 대상으로 한 연구결과와 다소 차이가 있는 것이며, 한국인을 위한 고유의 통계적 data로서 의미가 있는 것이라 하겠다. 이러한 차이를 보이는 원인을 유추해본다면, 인종에 따라 서로 다른 유전적 그리고 환경적인 요인의 차이에 의해 다형성이 미치는 영향력에 차이가 있기 때문으로 생각된다. 이는 당뇨병과 같은 질환이 특정한 한 두 개의 유전자 이상에 의해서 발생되기 보다는, 여러 유전자들이 상호 작용함으로써 발생할 뿐만 아니라 환경적인 요인의 영향을 많이 받기 때문에 인종이나 민독과 같은 인구 집단 사이에 유전인자의 차이가 생기는 것으로도 판단된다. 따라서 연구결과의 임상적인 의의를 확실하게 확인하기 위하여서는 향후 대상 환자 수와 대조군을 더욱 늘리고 다양한 HNF-$4{\alpha}$ 유전자 다형성을 후보로 하여 추가적인 연구를 할 필요가 있을 것으로 생각된다.

• 대한한의학방제학회지
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• 제14권2호
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• pp.76-85
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• 2006

Objectives : We Investigated the effect of Dioscoreae Rhizoma(山藥) on the change of the corticosterone and the rectal temperature(直腸溫渡) of the mice induced by starvation stress(創戰 스트레스). Methods : After administration of Dioscoreae Rhizoma (0.25g/kg, 0.5g, 1.0g/kg, 3g/kg) three times, mice were starved. The corticosterone and rectal temperature were measured after 36.5 hours starvation stress. Results : The plasma cortiosterone levels in the S-2, S-3 and S-4 group were decreased significantly comparing with the control group (P<0.01) after 36.5 hours starvation stress. and rectal temperature was decreased in the control goup comparing with the normal group, but there is no significant change in the Dioscoreae Rhizoma treated group. Conclusion : it might be recognized that Dioscoreae Rhizoma has preventive-effect against starvation stress and also it might be needed further study in various viewpoints. Objectives : This study was disegned to elucidate the short term effect of Rossa rugosae Radix on proliferation. differentiation and maturation of 3T3-L1 Preadipocyte. Methods: 3T3-L1 preadipocytes obtained from Korean Cell Line Bank were cultured in a D ulbecco’ s modified eagle medium(MEM) culture solution containing 10% fetal bovine serum(FBS) and various concentrations of aqueous extract of Rossa rugosae Radix.. The short term effect of the extract of Rossa rugosae Radix on proliferation. differentiation and maturation of 3T3-L1 preadipocytes were investigate after treatment for 24 hours by measuring MTT. Oil Red 0 and latate dehydrogenase activity.. Results: The Rossa rugosae Radix extract inhibited significantly the proliferation of 3T3-L1 preadipocytes and tended to increase latate dehydrogenase activity in the media of differentiated 3T3-L1 preadipocytes & matured 3T3-L1 preadipocytes. the extract also inhibit the lipid accumulation of differentiated 3T3-L1 preadipocytes & matuered 3T3-L1 preadipocytes. Conclusions: These results demonstrated that the Rossa rugosae Radjx extract inhibited the proliferation. differentiation and maturation of 3T3-L1 preadipocytes. suggesting that Rossa rugosae Radix has anti-obesity effect: however further in vivo study is needed to demonstrate its pharmacological effects.

• Journal of Acupuncture Research
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• 제32권3호
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• pp.41-51
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• 2015

Objectives : This study was performed to investigate the effects of $LR_3$ and $SP_6$ acupuncture on renal damage in streptozotocin(STZ)-induced diabetic mice. Methods : ICR male mice were stabilized for a week and divided into four groups: a normal mice group(N), no-acupuncture diabetic mice group(Control), $LR_3$ acupuncture diabetic mice group($LR_3$), and $SP_6$ acupuncture diabetic mice group($SP_6$). Diabetes was experimentally induced by intraperitoneal injection of STZ(150 mg/kg) in citrate buffer(pH 4.5). For two weeks, $LR_3$ and $SP_6$ acupunctures were administered bilaterally at each point once a day. After two weeks, the animals' weight was measured and they underwent a laparotomy. Serum glucose and blood urea nitrogen(BUN) were measured from the blood taken from the heart. We measured glucose, reactive oxygen species(ROS), peroxynitrite($ONOO^-$) and thiobarbituric acid reactive substances(TBARS) in the kidney and compared expression levels of superoxide dismutases(SOD), glutathione peroxidase(GPx), nuclear factor-kappa B(NF-${\kappa}B$), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase(iNOS) and Interleukin-1 beta(IL-$1{\beta}$). Results : BUN significantly decreased in $LR_3$, $SP_6$ compared to the control group. $LR_3$ showed significantly decreased glucose compared to the control group. $LR_3$, $SP_6$ significantly decreased in ROS and $ONOO^-$ compared to the control group. $LR_3$ significantly decreased in TBARS compared to the control group. $SP_6$ significantly increased in expressions of SOD-1, catalase, and GPx compared to the control group. $LR_3$, $SP_6$ significantly decreased in COX-2 compared to the control group. $SP_6$ significantly decreased in IL-$1{\beta}$ compared to the control group. Conclusions : This study suggests that $LR_3$ acupuncture may be effective in controlling glucose and lipid peroxidation and that $SP_6$ acupuncture may have anti-oxidative and anti-inflammatory effects on renal damage in STZ-induced diabetic mice.

• 대한간호학회지
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• 제30권3호
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• pp.741-749
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• 2000

This study was performed to investigate the relationship between reversed circadian blood pressure and risk factors of peripheral vascular disease in non-insulin-dependent diabetes mellitus (NIDDM) subjects. The subjects in this study were 18 NIDDM patients who were hospitalized in a medical unit of an university medical center located in Incheon, Korea, between November, 1998 and March, 1999. Blood pressure was measured with a mercury sphygmomanometer by 2 trained examiners every 2 hours during 24 hours. NIDDM subjects were divided into a dipper group and non-dipper group. Dippers are defined as those who show a mean nighttime blood pressure(BP) drop of more than 10% compared with daytime BP. Non-dippers are defined as those who show a mean nighttime BP drop of less than 10%, or an elevation in BP compared with daytime BP. Daytime BP included values obtained between 6 a.m. and 10 p.m. Night time BP included values obtained between 10 p.m. and 6 a.m. Data was analyzed by SPSS/PC package. Chi-square( $^2$) test was used for the comparison of sex between The dipper group and non-dipper group. Mann-Whitney test was used for comparisons of values of the risk factors of peripheral vascular disease and the frequency of complications of diabetes between the dipper group and non-dipper group. The results are as follows. There were no significant differences in daytime systolic, diastolic, and mean blood pressures between the dipper group and non-dipper group. However, night time systolic, diastolic, and mean blood pressures in the non-dipper group were significantly nigher than those in the dipper group (p=.021). There were no differences in sex, age, body, weight, duration of diabetes, serum lipid levels, BUN and HbA1c between the two groups. On the contrary, 87.5% of non-dipper group subjects showed having hypertension, 30% of dipper group subjects showed having hypertension and this difference was statistically significant (p=.018). All of the non-dipper group subjects (N=8) showed having at least one diabetic complication. However, 40% of the dipper group subjects (N=10) showed having no diabetic complication at all and this difference was also statistically significant (p=.049). There were no significant differences in frequency of nephropathy, neuropathy and retinopathy between the dipper group and non-dipper group.