Browse > Article
http://dx.doi.org/10.6116/kjh.2015.30.4.109.

Effects of Mume Fructus on the Rat Hypothyroidism Induced by PTU(6-n-propyl-2-thiouracil)  

Choi, Jae Young (Department of Korean Medicine, Daegu Haany University)
Roh, Seong Soo (Department of Korean Medicine, Daegu Haany University)
Park, Ji Ha (Department of Korean Medicine, Daegu Haany University)
Koo, Jin Suk (Dept. of Bioresource Science, Andong National University)
Seo, Bu Il (Department of Korean Medicine, Daegu Haany University)
Publication Information
The Korea Journal of Herbology / v.30, no.4, 2015 , pp. 109-119 More about this Journal
Abstract
Objectives : The aim of this study was to evaluate the effect of aqueous extracts of Mume Fructus(MF) on the 6-n-propyl-2-thiouracil(PTU)-induced rat hypothyroidism.Methods : Aqueous extracts of MF(yield = 19.38%) were administered, once per day for 42 days from 2 weeks before starting of PTU treatment as an oral dose of 300 and 150 ㎎/㎏(body weight), and hypothyroidism was induced by daily subcutaneous treatment of PTU 10 ㎎/㎏ for 28 days. The changes in the body weight, thyroid gland weights, liver weight, serum levels of thyroid hormone-thyroid stimulating hormone(TSH), tri-iodothyronine(T3) and thyroxine(T4), total cholesterol, low density lipoprotein(LDL), high density lipoprotein(HDL) and triglyceride (TG), aspartate aminotransferase(AST), alanine aminotransferase(ALT), liver antioxidant defense system-lipid peroxidation, H2O2, superoxide dismutase(SOD) and catalase(CAT) were examined with histopathology of thyroid glands and liver.Results : Results were compared with LevoT40.5 ㎎/㎏ treated rats. MF extracts recovered from the decreases in the body weight, liver weight, T3and T4, TG, liver CAT activities as results of PTU treatment. And MF extracts recovered from the increases of thyroid gland weights, TSH, HDL contents, liver H2O2, AST as results of PTU treatment. In addition, these PTU-induced histopathological changes in thyroid glands and liver related to hypothyroidism were dramatically decreased by treatment of both different dosages of MF extract, respectively.Conclusions : According to the above results, it is suggested that MF extracts have advantageous effects on the thyroid hormone productions with beneficial effects on the hypothyroidism related liver injuries mediated by the modulation on the antioxidant system.
Keywords
Mume Fructus; hypothyroidism; PTU(6-n-propyl-2-thiouracil);
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Reddy VS, Gouroju S, Suchitra MM, Suresh V, Sachan A, Srinivasa Rao PV, Bitla AR. Antioxidant defense in overt and subclinical hypothyroidism. Horm Metab Res. 2013 ; 45(10) : 754-8.   DOI   ScienceOn
2 Frost PH, Havel RJ. Rationale for use of nonhigh-density lipoprotein cholesterol rather than low-density lipoprotein cholesterol as a tool for lipoprotein cholesterol screening and assessment of risk and therapy. Am J Cardiol. 1998 ; 81 : 26B-31B.   DOI   ScienceOn
3 Jeong WJ, Park BH, Park CY, Ryu MS, Oh SJ, Woo JT, Kim SW, Yang IM, Kim JW, Choi YK, Paeng JR. Prevalence of subclinical hypothyroidism in hypercholesterolemic adults and influence of serum TSH on the serum total cholesterol level. Korean J Med. 2002 ; 62(2) : 187-93.
4 Das K, Chainy GB. Modulation of rat liver mitochondrial antioxidant defence system by thyroid hormone. Biochim Biophys Acta. 2001 ; 1537 : 1-13.   DOI   ScienceOn
5 Griffiths PD. Serum enzymes in disease of thyroid gland. J Clini Pathol. 1965 ; 18 : 660.   DOI
6 Ajala MO, Ogunro PS, Fasanmade OA. Relationship between liver function tests and thyroid hormones in thyroid disorders. Niger Postgrad Med J. 2013 ; 20(3) : 188-92.
7 Yang Y, Gordon CJ. Regulated hypothermia in the hypothyroid rat induced by administration of propylthiouracil. Am J Physiol. 1997 ; 272 : R1390-5.
8 Oner J, Kükner A, Oner H, Ozan E, Yekeler H. Effect of vitamin E on follicular cell proliferation and expression of apoptosis-associated factors in rats with 6-N-propyl-2-thiouracil-induced goitrogenesis. Folia Histochem Cytobiol. 2003 ; 41 : 213-17.
9 Mansourian AR. A review of literatures on the adverse effects of thyroid abnormalities and liver disorders : an overview on liver dysfunction and hypothyroidism. Pak J Biol Sci. 2013 ; 16(23) : 1641-52.   DOI
10 Ko BS, Park SK, Choi SB, Jun DW, Jang JS, Park SM. Hypoglycemic Effects of Crude Extracts of Prunus mume. J Korean Soc Food Sci Nutr. 2004 ; 33(6) : 951-7.   DOI   ScienceOn
11 Kim SM, Ku SK, Cho SY, Park SJ. Effects of Bupleuri Radix on the Rat Hypothyroidism induced by PTU(6-Prophyl, 2-thiouracil). Kor J Orient Physiol Pathol. 2012 ; 26(5) : 714-23.
12 Hong MJ, Lee BC, Ahn YM, Ahn SY. The Effects of Epimedii Herba on a Hypothyroidism Rat Model induced by PTU(6-Prophyl, 2-thiouracil). J Pharmacopuncture. 2011 ; 14(4) : 13-22.   DOI   ScienceOn
13 Lee SJ, Baek SH, Ahn SY, Lee BC, Ahn YM. Effects of Cistanche Deserticola on Thyroid Function in Hypothyroidism Rat Model induced by PTU(6-Prophyl, 2-thiouracil). Korean J Orient Physiol Pathol. 2011 ; 25(6) : 989-95.
14 Jung SE, Bae JH. The Effect of Prunus mume Extracts on the Growth of HepG2 and HeLa Cell Lines. Korean J Nutr. 2002 ; 35(4) : 439-45.
15 Roti E, Minelli R, Gardini E, Braverman LE. The use and misuse of thyroid hormone. Endocr Rev. 1993 ; 14 : 401-24.
16 Kim MS, Yoon CA, Cho YM, Jung HS, Shin CS, Park GS, Kim SY, Cho BY, Lee HG, Bloom SR. Changes in Plasma Leptin Levels Relating to ShortTerm Thyroid Manipulation in Rats. Endocrinol metabolism. 2002 ; 17(2) : 197-205.
17 Canaris GJ, Manowitz NR, Mayor G, Ridgway EC. The Colorado thyroid disease prevalence study. Arch Intern Med. 2000 ; 160 : 526-34.   DOI   ScienceOn
18 Guyton AC. Textbook of Medical Physiology. Philadelphia : WB Saunder's Company. 1986 : 906-8.
19 O’Hare E, Kim EM, Page D, Reid R. Effects of thyroxine treatment on histology and behavior using the methimazole model of congenital hypothyroidism in the rat. Neuroscience. 2015 ; 285 : 128-38.   DOI   ScienceOn
20 Nishikimi M, Rao NA, Yagi K The occurrence of superoxide anion in the reaction of reduced PMS and molecular oxygen. Biochem Biophys Res Comm. 1972 ; 46 : 849-54.   DOI   ScienceOn
21 Kakkar P, Das B, Viswanathan PN. Modified spectrophotometric assay of SOD. Indian J Biochem Biophys. 1984 ; 2 : 130-2.
22 Subudhi U, Das K, Paital B, Bhanja S, Chainy GB. Supplementation of curcumin and vitamin E enhances oxidative stress, but restores hepatic histoarchitecture in hypothyroid rats. Life Sci. 2009 ; 84 : 372-9.   DOI   ScienceOn
23 Andreoli TE, Carpenter CJ, Plum F, Smith L. Cecil Essentials of Medicine. Philadilphia : WB Saunder's Company. 1986 : 436-7.
24 Schmidt RF, Thews G. Human Physiology. Berlin Heidelberg : Springer-Verlag. 1989 : 385-6.
25 Koo JS, Kim BH, Seo BI. A clinical study on a patient with hypothyroidism. Kor J Herbol. 2014 ; 29(5) : 17-21.   DOI   ScienceOn
26 Kim DH, Choi JS, Kim CJ, Cho CS. The Effects of Daeyoungjeon(DYJ) on the Hypothyroidism in Rats. Kor J Herbol. 2007 ; 22(4) : 35-43.
27 Cho CS, Kim DB, Kim CJ. The Effects of Danggwisaeyeoktang on the Hypothyroidism of Rats. Kor J Herbol. 2007 ; 22(1) : 95-102.
28 Choi IG, Chae EY, Chang SK, Cho CS, Kim CJ. Effects of Jinmutang(JMT) on Hypothyroidism in Rats. J Korean Orient Int Med. 2006 ; 27(4) : 879-87.
29 KimSM. Effects of Ginseng Radix on the Rat Hypothyroidism Induced by PTU (6-n-propyl-2-thiouracil). Kor J Herbol. 2010 ; 25(3) : 11-8.
30 Kim SJ, Kim DC. Effects of Jaeumkanghwa-tang on the Rat Hypothyroidism induced by Propylthiouracil (PTU). J Orient Obstet Gynecol. 2014 ; 27(1) : 41-64.   DOI   ScienceOn
31 Jeon YH, Kwon JE, Kim MR. Study on Antioxidant and Cytotoxic Activities in Ethanol Extract from Prunus mume. J East Asian Soc Dietary Life. 2010 ; 20(5) : 751-8.
32 O’Connor JC, Frame SR, Ladics GS. Evaluation of a 15-day screening assay using intact male rats for identifying steroid biosynthesis inhibitors and thyroid modulators. Toxicol Sci. 2002 ; 69 : 79-91.   DOI   ScienceOn
33 The textbook compilation committee of Korean herbology. Korean herbology. Seoul : Younglimsa. 2011 : 676-7.
34 Sarandöl E, Taş S, Dirican M, Serdar Z. Oxidative stress and serum paraoxonase activity in experimental hypothyroidism : effect of vitamin E supplementation. Cell Biochem Funct. 2005 ; 23 : 1-8.   DOI   ScienceOn
35 Cooper DS. Antithyroid drugs. New Engl J Med. 1984 ; 311 : 1353-62.   DOI   ScienceOn
36 Kavutcu M, Canbolat O, Oztürk S, Olcay E, Ulutepe S, Ekinci C, Gökhun IH, Durak I. Reduced enzymatic antioxidant defense mechanism in kidney tissues from gentamicin-treated guinea pigs : effects of vitamins E and C. Nephron. 1996 ; 72 : 269-74.   DOI
37 Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ : Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 ; 193 : 265-75.
38 Jamall IS, Smith JC. Effects of cadmium on glutathione peroxidase, superoxidase dismutase and lipid peroxidation in the rat heart : a possible mechanism of cadmium cardiotoxicity. Toxicol Appl Pharmacol. 1985 ; 80 : 33-42.   DOI   ScienceOn
39 Aebi H. Catalase. In : Bergmeyer HU (Ed.), Methods in Enzymatic Analysis. New York : Academic Press Inc., 1974 : 673-86.
40 Pick E, Keisari Y. Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages--induction by multiple nonphagocytic stimuli. Cell Immunol. 1981 ; 59 : 301-18.   DOI   ScienceOn
41 Wiersinga WM. Thyroid hormone replacement therapy. Horm Res. 2001 ; 56 : 74-81.   DOI   ScienceOn
42 Doo HK. The sixth internal medicne of oriental medicine. Seoul : Oriental Medicine Research Institute. 1991 : 867-74.
43 Escobar-Morreale HF, Botella-Carretero JI, Morreale de Escobar G. Treatment of hypothyroidism with levothyroxine or a combination of levothyroxine plus L-triiodothyronine. Best Pract Res Clin Endocrinol Metab. 2015 ; 29(1) : 57-75.   DOI   ScienceOn
44 Cho BY. The clinical thyroidology. the 3rd editoin. Seoul : Korea Medicine. 2010 : 437-76.
45 Lanxiuping. Ziniziyinjianghuotang zhiliao gengnianqibumeiliaoxiao guancha. Shanxizhigong yixueyuanxuebao. 2003 ; 13(3) : 25.
46 Sanocka D, Miesel R, Jedrzejczak P, Kurpisz MK. Oxidative stress and male infertility. J Androl. 1996 ; 17(4) : 449-54.
47 Sahoo DK, Roy A, Chainy GB. Protective effects of vitamin E and curcumin on L-thyroxine-induced rat testicular oxidative stress. Chem Biol Interact. 2008 ; 176 : 121-8.   DOI   ScienceOn
48 Seo BI, Ju YS, Choi HY, Park JH, Roh SS, Koo JS, Kim JJ, Kim DY. Illustrated book of herbal plants in oriental medicine. Daegu : Daewondang. 2011 : 439-40.