Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Effect of Different Types of Dietary Fat on Renal Functions in Aged Rats

식이 지방의 종류가 흰쥐의 노화 과정 중 신장 기능에 미치는 영향

  • 김화영 (이화여자대학교 가정과학대학 식품영양학과)
  • Published : 1998.04.01
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Abstract

This study was performed to investigated the effect of dietary fat sources on renal senescence in aged rats. Seventeen month old male Sprague-Dawley rats were divided into 3 groups according to urinary protein excretion. Four month old rats were used as a control group. The rats were fed one of three different experimental diets ; 20% beef tallow, 20% corn oil 20% fish oil diet. They were fed experimental diets ad libitum for 16 weeks . The results are summarized as follows. Serum lipid concentrations were higher in aged rats than in control rats, with the beef tallow group showing the highest level, followed by the corn oil and fish oil groups. Old rats showed higher HDL and lower LDL levels than the control groups. Age and dietary fat had no effect on VLDL. GFR for the both age groups were increased with experimental period with the beef tallow group showing the highest value. Urinary protein excretion was also increased with experimental period in both age groups. There was a large increase in urinary protein in old rats that were fed beef tallow and corn oil, but not in old rats fed fish oil. On the contrary , the effect of dietary fat on urinary protein was not found in control groups. There was individual susceptibility in the effect of dietary fat on urinary protein. Old rats fed beef tallow with high initial urinary protein showed highest increase, but , the change was not significant in rats with a low initial value . It was also found that the increase was kept low in rats of the fish oil group with high initial urinary protein. The corn oil group showed in between values. There were no differences in urine and renal tissue concentrations of TXB2 . Aged rats showed a tendency of having higher urinary PGE2 excretion and lower renal cortex content. Since higher PGE2 excretion was reported to be associated with decreased renal function, this might suggest that the aged rats show renal function reduction. Light microscopic examination showed that glomerular segmental sclerosis, mesangial matrix expansion and tubular atrophy were more frequently present in aged rats, and that these changes were more significant in the beef tallow group, followed by corn oil and fish oil groups. The percentage of urinary protein excretion was increased in aged rats in association with increased glomerular sclerosis and mesangial matrix . This change could be partly due to a change in eicosanoids metabolism . Therefore, modification of dietary fat could affect the eicosanoids metabolism in kidney and renal senescence.

Keywords

References

  1. N. Engl. J. Med. v.318 The progression of renal disease Klahr,S.;Schreiner,G.;Ichikawa,I.
  2. Proc. Soc. Exp. Biol. Med. v.230 Diet and kidney disease : the role of dietary fatty acids Schreiner,G.F.;Klahr,S.
  3. Am. J. Kid. Dis. v.5 The role of nutritional factors in the course of experimental renal failure Laouari,D.;Keenkeche,C.
  4. Korean J. Nutr. v.29 no.10 Effect of dietary protein level on renal functions and structure in uninephrectomized aging model in rat Kim,W.Y.;Lee,H.S.;Jeong,H.J.
  5. Korean J. Nutr. v.29 no.10 Effect of dietary protein level on renal function : Lipid and eicosanoids metabolism in uninephrectomized aging model in rats Kim,W.Y.;Lee,H.S.
  6. Isr. J. Med. Sci. v.29 Role of lipids in the progression of renal disease in chronic renal failure : evidence from animal studies and pathogenesis Shohat,J.;Boner,G.
  7. J. Immunol. v.134 A fish oil diet rich in eicosapentoic acid reduces cyclooxygenase metabolism and suppresses lupus in MRL-lpr mice Kelley,V.E.;Ferretti,A.;Izui,S.(et al.)
  8. Prostaglandins v.31 The protective effect of dietary fish oil on murine lupus Robinson,D.R.;Prickett,J.D.;Polisson,R.(et al.)
  9. J. Nutr. v.107 Ad Hoc committe on standard for nutritional studies Report of the American Institute of Nutrition
  10. Korean J. Nutr. v.25 no.10 The effect of age and dietary protein level on Ca metabolism in rats Lee,J.A.;Jang,Y.A.;Kim,W.Y.
  11. Prostaglandins v.22 Measurement of prostaglandins in embryonic tissue using radioimmunoassay Kein,K.L.;Scott,W.J.;Clark,K.E.
  12. Am. J. Clin. Pathol. v.53 A colorimetric method for determination of total serum lipids based on the sulfophosphovanillin reaction Frings,C.S.;Dunn,R.T.
  13. standard method of clinical chemistry Total and free cholesterol Zak,B.
  14. Clin. Chem. v.21 A manual colorimetric assay of triglyceridein serum Biggs,H.G.;Erickson,J.M.;Moorehead,W.R.
  15. Electrophoresis in the seperation of biological macromolecules Ga$`{a}$l,$"{O}$.;Medgyesi,G.A.;Vereczkey,L.
  16. Haw's physiological chemistry(4th edition) Oser,B.L.
  17. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry,O.H.;Resebrouh,N.J.;Randal,R.J.
  18. Kidney Int. v.29 Prostaglandins and other arachidonic acid metabolites in the kidney Schlondorff,D.;Arvaillou,R.
  19. Prostaglandins v.34 Role of prostaglandin in the cortial distribution of renal blood flow following reductions in renal perfusion pressure Opgenorth,T.J.;Fiksen-Olsen,M.J.;Romero,J.C.
  20. Am. J. Clin. Nutr. v.62 Incorporation of dietary n-3 fatty acids into the fatty acids of human addipose tissue and plasma lipid classes Leaf,D.A.;Connor,W.E.;Barstad,L.;Sexton,G.
  21. Lab. Invest. v.60 Induction of glomerulosclerosis by dietary lipids : a functional and morphologic study in the rat Gr$"{o}$ne,H.J.;Walli,A.;Gr$"{o}$ne,E.;Niedmann,P.;Thiery,J.
  22. Am. J. Kidney Dis. v.15 Renal vadilatory response to intrvenous glycine in the agig rat kidney Baylis,C.;Fredericks,M.;Wilson,C.;Munger,K.;Collins,R.
  23. J. Gerontol. v.24 The effect of age on renal function of female rats Gregory,J.G.;Barrows,C.H.
  24. Lab. Invest. v.46 Adriamycin-induces nephrotic syndrome in rats Brtani,T.;Poggi,A.;Pozzoni,R.(et al.)
  25. Kidney Int. v.44 Studies of alteration of hepatic cholesterol metabolism in puromycin-induced nephrotic syndrome on rats Challa,A.;Pandak,W.M.;Heuman,D.M.
  26. Br. Med. J. v.293 Does sodium restriction lower blood pressure? Grobbee,D.E.;Hofman,A.
  27. Nephron. v.46 Urinary prostaglandins and thromboxane in patients with chronic glomerulonephritis Niwa,T.;Maeda,K.;Shibata,M.
  28. Clin. Nephrol. v.15 Urinary prostaglandin E₂ in chronic renal disease Blum,M.;Bauminger,S.;Algueti,A.;Kisch,E.;Ayalon,D.
  29. Kidney Int. v.38 Role of enhanced glomerular synthesis of thromboxane A2 in progressive kidney disease Salvati,P.;Ferti,C.;Ferrario,R.G.;Lamberti,E.(et al.)
  30. Isr. J. Med. Sci. v.29 Role of lipids in the progression of renal disease in chronic renal failure : evidence from animal studies and pathogenesis Shoat,J.;Boner,G.
  31. J. Gerontol. v.32 Pathological changes during aging in barrier-reared Fisher 344 male rats Colemann,G.L.;Barthold,S.W.;Osbaliston,G.W.;Foster,S.J.;Jonas,A.M.