• Title/Summary/Keyword: sequential properties

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Structural Properties of Naked and Covered Barley Starches (쌀보리와 겉보리 전분의 분자구조적 성질)

  • Choi, Jun-Bok;Lee, Shin-Young
    • Korean Journal of Food Science and Technology
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    • v.21 no.1
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    • pp.80-85
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    • 1989
  • The structural properties of covered and naked barley starches and those components were investigated. ${\beta}-amylosis\;limit(%)$ of covered and naked barley starches were 58.6 and 56.3%, respectively and those of their amyloses and amylopectins were 87.0, 77.7, 57.6 and 52.0%, respectively. The ratios of outer chain length ${\overline{OCL}}$ and inner chain length${\overline{ICL}}$ for amylopectins of covered and naked barley were about 2.2:1 and 2.0:1, respectively. The elution curves by Sephadex G-75 after debranching starches with pullulanase were similar patterns for two starches and yielded two peaks consisting of amylose component${\overline{dp}}>55$ and bimodal amylopectin components of ${\overline{dp}_(35-45)$ and ${\overline{dp}}(10-20)$ hydrolysates. Also, hydrolysis products by sequential hydrolysis with pullulanase and ${\beta}-amylase$ contained 0.03-0.5% non-hydrolyzed peak components of ${\overline{dp}}>55$. The elution profiles of ${\beta}-amylase$ hydrolysates yielded two peaks consisting of the inner components${\overline{dp}}>55$ and the outer chain components of amylopectin${\overline{dp}}>10$ regardless of samples. However, ${\overline{dp}}$ distributions of ${\overline{dp}>55}$ hydrolysates exhibited the significant differences due to the varieties.

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Antioxidation Activities of Organic Solvent Fractions Obtained from Seaweed, Hizikia fusiformis (톳(Hizikia fusiformis)에서부터 분리된 유기용매 분획물의 항산화활성)

  • Kim, Min-Jeong;Lee, Hye-Hyeon;Seo, Min-Jeong;Kang, Byoung-Won;Park, Jeong Uck;Jeong, Yong Kee
    • Journal of Life Science
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    • v.23 no.3
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    • pp.361-367
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    • 2013
  • Hizikia fusiformis has been widely used in Oriental herbal medicine and health food. To identify antioxidation properties that contain natural bioactive substances, we investigated the distribution of active compounds existing in batches of organic solvent fractionation. A dried form of H. fusiformis was subjected to sequential fractionation using n-hexane, ethyl acetate, n-BuOH, and aqueous n-BuOH. The results showed that among the four isolated fractions, the n-BuOH fraction showed the highest antioxidation activities. The n-BuOH fraction was applied to reserve-phase silica gel column chromatography, which produced three fractions: BA, BB, and BC. Among these fractions, BB showed the highest antioxidation activities, which increased in a concentration-dependent manner. At a concentration of 1.0 mg/ml n-BuOH fraction, the activities of DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging and reducing power were approximately $45{\pm}0.14%$ and $1.34{\pm}0.23$, respectively. In addition, the activities of ${\beta}$-carotene-linoleic acid, hydrogen peroxide scavenging, and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) radical scavenging were $76{\pm}0.12%$, $82{\pm}0.06%$, and $65{\pm}0.17%$, respectively. These findings suggest that the BB fraction contains potent antioxidation properties and that it could be used in the production of natural and functional foods.

Evaluation of Fluoride Distribution, Fate and Transport Characteristics in Soils (토양 중 불소 분포 및 거동 특성 평가)

  • Lim, Ga-Hee;Lee, Hong-Gil;Kim, Hyoung-Seop;Noh, Hoe-Jung;Ko, Hyoung-Wook;Kim, Ji-In;Jo, Hun-Je;Kim, Hyun-Koo
    • Journal of Soil and Groundwater Environment
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    • v.23 no.6
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    • pp.90-103
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    • 2018
  • Although fluoride is an essential trace element, ingestion of excessive amount of fluoride could have detrimental effect on human health. Generally, the bioavailability of fluoride in soils was low, but it could be harmful to the environment depending on the soil properties. Therefore, it is necessary to understand the concentration distribution, and fate and transport characteristics of fluoride to establish a resonable management strategy for fluoride pollution. This study was conducted to evaluate nationwide fluoride distribution in soils in Korea, as well as its fate and transport characteristics. The average background concentration was 204.5 (15.3~504.8) mg/kg, which is lower than the values of foreign soils. For the three regions of different land use, the average concentration was 229.6 mg/kg in region 1, 195.7 mg/kg in region 2, and 273.4 mg/kg in region 3. The concentration of fluoride was the highest in soils from Youngnam block within tectonic structure derived from metamorphic rocks. The results of sequential extraction to access F bioavailability showed fluoride in soils mainly existed as a residual form, which suggests the bioavailability of fluoride was relatively low. Soil properties such as soil pH, CEC, and clay content were found to affect F bioavailability of soil.

A Study on the Changes in Surface Properties According to Post-treatment of SLA 3D Printing Materials (SLA 3D 프린팅 소재의 후처리에 따른 표면특성 변화 연구)

  • Bae, Seo Jun;Im, Do Jin
    • Korean Chemical Engineering Research
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    • v.60 no.1
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    • pp.132-138
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    • 2022
  • In this study, a basic study was performed to systematically compare the changes in surface properties according to the post-processing method of the stereolithography (SLA) printing method, which is a photocuring 3D printing method, and to provide information on the post-processing method suitable for the application. Although it was possible to improve some of the transparency of the SLA-type output by regularly changing the irregular microstructure of the surface through polishing, it was difficult to secure sufficient transparency like glass. The change in contact angle characteristics due to grinding showed a tendency to slightly increase as the grinding time increased and the particle size of the sandpaper used was small, but the variation between samples was large and the average contact angle was 77~90°, showing no statistically significant difference. Surface treatment methods other than polishing were tried, and it was confirmed that it was possible to easily and simply improve the transparency by applying a commercially available vehicle scratch remover or silicone oil. In addition, a method for securing high transparency such as glass by using a scratch remover after sequential grinding while reducing the particle size of the sandpaper was proposed. Finally, even after surface treatment through polishing and various methods, it was difficult to secure a contact angle of 90° or more.

Failure Properties of Common Tendon Origins at the Human Elbow after Static and Repetitive Loading (정적 및 반복하중 시의 주관절 Tendon의 파괴 물성치 측정)

  • Han, Jeong-Su;Lee, Gwan-Hui;Yu, Jae-Yeong
    • Journal of Biomedical Engineering Research
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    • v.19 no.4
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    • pp.393-401
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    • 1998
  • Based on clinical observations, it is suspected that the bone-tendon origin is the site where piratical failure, leading to pathophysiological changes in the humeral epicondyle after repetitive loading, is initiated Mechanical properties and failure patterns of the common extensor and flexor tendons of the humeral epicondyle under static and repetitive loading have not been well documented. Our goal was to determine mechanical properties of failure strength and strain changes, to correlate strain changes and the number of cyclic repetitions, and to identify the failure pattern of bone-tendon specimens of common extensor and flexor tendons of the humeral epicondyle. Mechnaical properties of human cadaver bone-tendon specimens of the common extensor and flexor tendons of the humeral epicondyle were tested under two different loading rates. No statistically significant difference in ultimate tensile strength was found between male and female specimens or between slow (10 mm/sec) and fast elongation (100 mm/sec) rates. However, a statistically significant difference in ultimate tensile strength between the common extensor (1190.0 N/$cm^2{\pm}$388.8) and flexor 1922.0 N/$cm^2{\pm}$764.4)tendons was found (p<0.05). When loads of 25%, 33%, and 41% of the ultimate tensile strength of their contralateral sides were applied, the number of cycles required to reach 24% strain change for the common extersor and flexor tendons were approximately 8,893, 1,907, and 410, respectively. The relationship between cycles and loads was correlated ($R^2$=0.46) Histological observation showed that complete or partial failure after tensile or cyclic loadings occurred at the transitional zone, which is the uncalcified fibrocartilage zone between tendon and bone of the humeral epicondyle. Sequential histological sections revealed that failure initiated at the upper, medial aspect of the extensor carpi radialis brevis tendon origin. Biomechanical and hstological data obtained in this study indicated that the uncalcified fibrocartilage zone at the bone-tendon origin of the common extensor and flexor tendons is the weak anatomical structure of the humeral epicondyle.

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Material Characteristics of White Wares from Yeongdong Province, Gangwon-do: Gangneung and Donghae Kiln Sites (강원 영동지역 백자의 재료과학적 특성 연구: 강릉 남양리 백자가마터와 동해 발한동2(사문동) 가마터를 중심으로)

  • Lee, Byeong Hoon;So, Myoung-Gi
    • Journal of Conservation Science
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    • v.31 no.3
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    • pp.181-192
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    • 2015
  • This study aims to examine production technique of twelve white wares from the Gangneung Namyang-ri and Donghae Balhan-dong2(Samun-dong) kiln sites, Kangwon Province and characteristics of the used materials, and to find a correlation among materials of the excavated white wares. X-ray fluorescence sequential spectroscopy(XRF), X-ray diffraction(XRD), Dilatometer and Inductively coupled plasma mass spectrometry(ICP-MS), Inductively coupled plasma automic emission spectrometer(ICP-AES) were applied to determine the chemical composition, crystalline phase of samples, firing temperatures, trace elements and rare earth elements. When analyzing body crystalline phases of the white wares using the XRD method, quartz and mullite were extracted from all the samples. Though firing temperature of each sample was different, they were mostly fired at a temperature below $1200^{\circ}C$. Analyzing the excavated white wares using the Seger formula, compositional properties of white wares in Gangneung Namyang-ri kiln showed diffrently from the Donghae Balhan-dong2(Samun-dong) kiln. The body of white wares from Gangneung Namyang-ri kiln have higher raito of $RO_2$ and $RO+R_2O$ than of white wares from Donghae Balhan-dong2(Samun-dong) kiln site. The white wares from Gangneung Namyang-ri kiln and Donghae Balhan-dong2(Samun-dong) kiln were made of host rocks of the different geological origin, according to the result of rare earth elements analysis.

Screening of Hemicellulose Oligosaccharides and Preparation of the Recipe for Modified MRS Medium by the Replacement of Carbon Source (Hemicellulose계열 올리고당 탐색 및 탄소원 대체에 의한 장내세균 생육활성용 신규 MRS배지의 조제)

  • Lee, Hee-Jung;Park, Gwi-Gun
    • Journal of Applied Biological Chemistry
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    • v.51 no.6
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    • pp.272-276
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    • 2008
  • Purification and some properties of Xylogone sphaerospora ${\beta}$-mannanase were reprevious previous paper. Locust bean gum galactomannan was hydrolyzed by the purified ${\beta}$-mannanase, and then the hydrolysates was separated by activated carbon column chromatography. The main hydrolysates were composed of D.P. (Degree of Polymerization) 4 and 6 galactosyl mannooligosaccharides. For elucidate the structure of D.P 4 and 6 galactosyl mannooligosaccharides, sequential enzymatic action was performed. D.P 4 and 6 were identified as ${Gal^2}{Man_3}\;(6^2-mono-O-{\alpha}-D-galactopyranosyl-4-O-{\beta}-D-mannotriose)$ and ${Gal^2}{Man_5}\;(6^2-mono-O-{\alpha}-D-galacto- pyranosyl-4-O-{\beta}-D-mannopentaose)$. To investigate the effects of locust bean gum galactosyl mannooligosaccharides on in vitro growth of Bifidobacterium longum, B. bifidum, B. infantis, B. adolescentis, B. animalis, B. auglutum and B. breve. Bifidobacterium spp. were cultivated individually on the modified-MRS medium containing carbon source such as D.P. 4 and D.P. 6 galactosyl mannooligosaccharides, respectively. B. longum and B. bifidum grew up to-fold and 6.6-fold more effectively by the treatment of D.P. 6 galactosyl mannooligosaccharides, compared to those of standard MRS medium. Especially, D.P. 6 was more effective than D.P. 4 galactosyl mannooligosaccharide on the growth of Bifidobacterium spp.

Manufacturing Techniques and the Conservation Treatment of Chimi - (Ridge-end tile) Excavated from the Beopcheonsa Temple Site, Wonju - (원주 법천사지 토제 치미의 제작기법과 보존처리)

  • Lee, Seung Gang;Jo, Seong Yeon;Huh, Il Kwon
    • Journal of Conservation Science
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    • v.35 no.5
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    • pp.518-527
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    • 2019
  • This investion studies the manufacturing techniques of chimi(ridge-end roof tiles based on the) fragments excavated from the Wonju Beopcheonsa temple site(Historic site No. 466) and aids in the conservation of the fragments. The results of the investigation are categorized into the production of the body parts, the wing and the feather attachment, the production of the decorative parts, the scratches in the upper and lower part, the perforations connecting the upper and lower parts, and the formative features(bending phenomenon). The procedures in the conservation treatment of the chimi was performed in a sequential order beginning with a preliminary examination, followed by the removal of foreign substances, coating, joining and restoration, and color retouching. A three-dimensional scanning data was employed to restore the missing parts after adhesion to determine the location, size, and angle of the original shape. The restored chimi measures 118 cm in height and weighs 121 kg, which makes it the fifth largest in size among any chimi(including restored) in Korea. We expect that the pointed feathers will make the chimi from the Beopcheonsa temple site a rare reference as no specimens with these features have been found in Korea until now.

Concentration and Properties of Particulate Matters (PM10 and PM2.5) in the Seoul Metropolitan (서울시 지하철 시스템 내의 입자상물질(PM10, PM2.5) 농도 특성)

  • Lee, Tae-Jung;Lim, Hyoji;Kim, Shin-Do;Park, Duck-Shin;Kim, Dong-Sool
    • Journal of Korean Society for Atmospheric Environment
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    • v.31 no.2
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    • pp.164-172
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    • 2015
  • Seoul subway plays an important part for the public transportation service in Seoul metropolitan area. As the subway system is typically a closed environment, frequent air pollution problems occurred and passengers get malhealth impact. Especially particulate matters (PM) is well known as one of the major pollutants in subway environments. The purpose of this study was to compare the concentrations of $PM_{10}$ and $PM_{2.5}$ in the Seoul subway system and to provide fundamental data in order to management of subway system. $PM_{10}$ and $PM_{2.5}$ samples were collected in the M station platform and tunnel of Subway Line 4 in Seoul metropolitan and in an outdoor location close to it from Apr. 21, 2010~Oct. 27, 2013. The samples collected on teflon filters using $PM_{10}$ and $PM_{2.5}$ mini-volume portable samplers and PM sequential sampler. The PM contributions were $48.6{\mu}g/m^3$ (outdoor), $84.6{\mu}g/m^3$ (platform) and $204.8{\mu}g/m^3$ (tunnel) for $PM_{10}$, and $34.6{\mu}g/m^3$ (outdoor), $49.7{\mu}g/m^3$ (platform) and $83.1{\mu}g/m^3$ (tunnel) for $PM_{2.5}$. The $PM_{10}$ levels inside stations and outdoors are poorly correlated, indicating that $PM_{10}$ levels in the metro system are mainly influenced by internal sources. In this study, we compared PM concentrations before and after operation of ventilation and Electrostatic Precipitator (EP). Despite the increased PM concentration at outdoor, $PM_{10}$ concentration at platform and tunnel showed the 31.2% and 32.3% reduction efficiency after operation the reduction system. The overall results of this study suggest that the installation and operation of the ventilating system and EP should have served as one of the important components for maintaining the air quality in the subway system.

Anticancer Activity of Acanthopanax trifoliatus (L) Merr Extracts is Associated with Inhibition of NF-κB Activity and Decreased Erk1/2 and Akt Phosphorylation

  • Wang, Hua-Qian;Li, Dong-Li;Lu, Yu-Jing;Cui, Xiao-Xing;Zhou, Xiao-Fen;Lin, Wei-Ping;Conney, Allan H.;Zhang, Kun;Du, Zhi-Yun;Zheng, Xi
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.21
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    • pp.9341-9346
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    • 2014
  • Acanthopanax trifoliatus (L) Merr (AT) is commonly used as an herbal medicine and edible plant in some areas of China and other Asian countries. AT is thought to have anticancer effects, but potential mechanisms remain unknown. To assess the anticancer properties of AT, we exposed prostate cancer cells to AT extracts and assessed cell proliferation and signaling pathways. An ethanol extract of AT was suspended in water followed by sequential extraction with petroleum ether, ethyl acetate and n-butanol. PC-3 cells were treated with different concentrations of each extract and cell viability was determined by the MTT and trypan blue exclusion assays. The ethyl acetate extract of the ethanol extract had a stronger inhibitory effect on growth and a stronger stimulatory effect on apoptosis than any of the other extracts. Mechanistic studies demonstrated that the ethyl acetate extract suppressed the transcriptional activity of NF-${\kappa}B$, increased the level of caspase-3, and decreased the levels of phospho-Erk1/2 and phospho-Akt. This is the first report on the anticancer activity of AT in cultured human prostate cancer cells. The results suggest that AT can provide a plant-based medicine for the treatment or prevention of prostate cancer.