• Title/Summary/Keyword: sequence length

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Complete Chloroplast DNA Sequence from a Korean Endemic Genus, Megaleranthis saniculifolia, and Its Evolutionary Implications

  • Kim, Young-Kyu;Park, Chong-wook;Kim, Ki-Joong
    • Molecules and Cells
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    • v.27 no.3
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    • pp.365-381
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    • 2009
  • The chloroplast DNA sequences of Megaleranthis saniculifolia, an endemic and monotypic endangered plant species, were completed in this study (GenBank FJ597983). The genome is 159,924 bp in length. It harbors a pair of IR regions consisting of 26,608 bp each. The lengths of the LSC and SSC regions are 88,326 bp and 18,382 bp, respectively. The structural organizations, gene and intron contents, gene orders, AT contents, codon usages, and transcription units of the Megaleranthis chloroplast genome are similar to those of typical land plant cp DNAs. However, the detailed features of Megaleranthis chloroplast genomes are substantially different from that of Ranunculus, which belongs to the same family, the Ranunculaceae. First, the Megaleranthis cp DNA was 4,797 bp longer than that of Ranunculus due to an expanded IR region into the SSC region and duplicated sequence elements in several spacer regions of the Megaleranthis cp genome. Second, the chloroplast genomes of Megaleranthis and Ranunculus evidence 5.6% sequence divergence in the coding regions, 8.9% sequence divergence in the intron regions, and 18.7% sequence divergence in the intergenic spacer regions, respectively. In both the coding and noncoding regions, average nucleotide substitution rates differed markedly, depending on the genome position. Our data strongly implicate the positional effects of the evolutionary modes of chloroplast genes. The genes evidencing higher levels of base substitutions also have higher incidences of indel mutations and low Ka/Ks ratios. A total of 54 simple sequence repeat loci were identified from the Megaleranthis cp genome. The existence of rich cp SSR loci in the Megaleranthis cp genome provides a rare opportunity to study the population genetic structures of this endangered species. Our phylogenetic trees based on the two independent markers, the nuclear ITS and chloroplast MatK sequences, strongly support the inclusion of the Megaleranthis to the Trollius. Therefore, our molecular trees support Ohwi's original treatment of Megaleranthis saniculifolia to Trollius chosenensis Ohwi.

Performance Comparison and Improvement of STDR/SSTDR Schemes Using Various Sequences (여러 가지 수열을 적용한 STDR/SSTDR 기법의 성능 비교 및 개선)

  • Han, Jeong Jae;Park, So Ryoung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.39A no.11
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    • pp.637-644
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    • 2014
  • This paper investigates the detection performance of fault location using STDR(sequence time domain reflectometry) and SSTDR(spread spectrum time domain reflectometry) with various length and types of sequences, and then, proposes an improved detection technique by eliminating the injected signal in SSTDR. The detection error rates are compared and analyzed in power line channel model with various fault locations, fault types, and spreading sequences such as m-sequence, binary Barker sequence, and 4-phase Frank sequence. It is shown that the proposed technique is able to improve the detection performance obviously when the reflected signal is weak or the fault location is extremely close.

Algorithmic Generation of Self-Similar Network Traffic Based on SRA (SRA 알고리즘을 이용한 Self-Similar 네트워크 Traffic의 생성)

  • Jeong HaeDuck J.;Lee JongSuk R.
    • The KIPS Transactions:PartC
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    • v.12C no.2 s.98
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    • pp.281-288
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    • 2005
  • It is generally accepted that self-similar (or fractal) Processes may provide better models for teletraffic in modem computer networks than Poisson processes. f this is not taken into account, it can lead to inaccurate conclusions about performance of computer networks. Thus, an important requirement for conducting simulation studies of telecommunication networks is the ability to generate long synthetic stochastic self-similar sequences. A generator of pseudo-random self similar sequences, based on the SRA (successive random addition) method, is implemented and analysed in this paper. Properties of this generator were experimentally studied in the sense of its statistical accuracy and the time required to produce sequences of a given (long) length. This generator shows acceptable level of accuracy of the output data (in the sense of relative accuracy of the Hurst parameter) and is fast. The theoretical algorithmic complexity is O(n).

A Fast Map-matching Method using a Laser Range Finder

  • Moon, Jung-Hyun;You, Bum-Jae;Oh, Sang-Rok;Kim, Hag-bae
    • 제어로봇시스템학회:학술대회논문집
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    • 2002.10a
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    • pp.38.4-38
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    • 2002
  • We propose a fast map-matching algorithm based on the length and the slope for the sequence of lines extracted from a laser range finder and a map. After finding two feature set from laser data and a map, the position and heading of the mobile robot can be determined exactly.

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Nucleotide Sequence Analysis and Secondary Structure Modeling of the 3'-Noncoding Regions of Two Korean Strains of Turnip Mosaic Virus (순무 모자이크 바이러스 두 한국계통의 3' 말단 비번역부위에 대한 염기서열분석 및 2차구조 모델링)

  • 최장경;류기현;최국선;박원목
    • Korean Journal Plant Pathology
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    • v.11 no.3
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    • pp.271-277
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    • 1995
  • The RNA nucleotide sequences of the 3/-noncoding regions (3'-NCRs) of two Korean strains of turnip mosaic virus (TuMV), Ca and cqs, have been determined from their cDNA clones that encompassed the 3'-terminal regions of the viral genomic RNAs. The 3'-NCRs of both strains were 209 nucleotides long, terminated with GAC residues and poly (A) tails. The potential polyadenylational signal motif, UAUGU, was located 140 nucleotides upstream from the poly (A) tail in each of the virus. A highly conserved hexanucleotide sequence [A G U G A/U G/C], which was common in the 3'-NCRs of the potyvirus RNAs, was also found at the regions of 119 bases upstream from the 3'-end. Comparison of the 3'-NCRs of the two Korean isolates with those of four strains from Canada, China and Japan showed significantly identical genotypes (94.3∼99.5%). The secondary structure of three loops with long stems was found within the 3'-NCRs by sequence analysis. The substituted bases in the region among the six TuMV strains did not alter their secondary structures. Length of the 3'-NCRs of the know 11 potyviral RNAs and TuMV RNAs was different from one another and their nucleotide sequences showed 55.7% to 24.0% of homology. The 3'-NCR, therefore, is considered to be useful for phylogenetic studies in potyviruses.

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Analysis and Identification of Expressed Sequence Tags in Hairy Root Induced from Korean Ginseng (Panax ginseng C. A. Meyer)

  • Yang, Deok-Chun;In, Jun-Gyo
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.2
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    • pp.154-162
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    • 2004
  • Hairy roots were induced from Korean ginseng (Panax ginseng C. A. Meyer) root explants and studied for their gene expression. A total of 3,000 ESTs (expressed sequence tags) from ginseng hairy root were determined and about 2,700 ESTs have a length of readable sequence, which result in 1,352 unique ESTs sequences. The 879 ESTs showed significant similarities to known nucleotide or amino acid sequences in other plant species, which were divided into eleven categories depending upon gene function. The remaining 473 sequences showed no significant matches, which are likely to be transcripts or to be matched to other organisms. The results indicated that the analysis of the ginseng hairy root ESTs by partial sequencing of random cDNA clones may be an efficient approach to isolate genes that are functional in ginseng root in a large scale. Our extensive EST analysis of genes expressed in ginseng hairy root not only contributes to the understanding of the dynamics of genome expression patterns in root organ but also adds data to the repertoire of all genomic genes.

Nucleotide sequence analysis of the 5S ribosomal RNA gene of the mushroom tricholoma matsutake

  • Hwang, Seon-Kap;Kim, Jong-Guk
    • Journal of Microbiology
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    • v.33 no.2
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    • pp.136-141
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    • 1995
  • From a cluster of structural rRNA genes which has previsouly been cloned (Hwang and Kim, in submission; J. Microbiol. Biotechnol.), a 1.0-kb Eco RI fragment of DNA which shows significant homology to the 25S and rRNA s of Tricholoma matsutake was used for sequence analysis. Nucleotide sequence was bidirectionally determined using delection series of the DNA fragment. Comparing the resultant 1016-base sequence with sequences in the database, both the 3'end of 25S-rRNA gene and 5S rRNA gene were searched. The 5S rRNA gene is 118-bp in length and is located 158-bp downstream of 3'end of the 25S rRNA gene. IGSI and IGS2 (partial) sequences are also contained in the fragment. Multiple alignment of the 5S rRNA sequences was carried out with 5S rRNA sequences from some members of the subdivision Basidiomycotina obtained from the database. Polygenetic analysis with distance matrix established by Kimura's 2-parameter method and phylogenetic tree by UPGMA method proposed that T. matsutake is closely related to efibulobasidium allbescens. Secondary structure of 5S rRNA was also hypothesized to show similar topology with its generally accepted eukaryotic counterpart.

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Cloning and Characterization of DAP10 homologue gene from Olive Flounder, Paralichthys olivaceus

  • Park, Chan-Il;Kim, Mu-Chan;Hwang, Jee-Youn;Kim, Ki-Hyuk;Kim, Joo-Won
    • Journal of fish pathology
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    • v.19 no.3
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    • pp.227-233
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    • 2006
  • Olive flounder immunoreceptor DAP10 homologue cDNA was cloned from a peripheral blood lymphocytes (PBLs) cDNA library. The length of the olive flounder DAP10 cDNA is 473bp and it contains an open reading frame of 234bp. The predicted polypeptide sequence is 78 amino acids, consisting of a 22-amino acid leader, an 11-amino acid extracellular domain, a 21-amino acid transmembrane segment, and a 24-amino acid cytoplasmic domain. The amino acid sequence of olive flounder DAP10 has 56%, 50%, 32%, 31%, and 31% sequence identity with zebrafish DAP10, catfish DAP10, cattle DAP10, rat DAP10 and Monkey DAP10, respectively. Olive flounder DAP10 has a conserved aspartic acid in the transmembrane domain and a phophatidylinositol-3 kinase-binding site (YxxM/V) in the cytoplasmic region. Genomic organization reveals that olive flounder DAP10 comprises five exons and four introns. A phylogenetic analysis based on the deduced amino acid sequence grouped the olive flounder DAP10 with other species DAP10. In RT-PCR analysis, DAP10 transcripts were detected predominantly in PBLs, kidney, spleen and intestine.

Coreference Resolution using Hierarchical Pointer Networks (계층적 포인터 네트워크를 이용한 상호참조해결)

  • Park, Cheoneum;Lee, Changki
    • KIISE Transactions on Computing Practices
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    • v.23 no.9
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    • pp.542-549
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    • 2017
  • Sequence-to-sequence models and similar pointer networks suffer from performance degradation when an input is composed of multiple sentences or when the length of the input sentence is long. To solve this problem, this paper proposes a hierarchical pointer network model that uses both the word level and sentence level information to encode input sequences composed of several sentences at the word level and sentence level. We propose a hierarchical pointer network based coreference resolution that performs a coreference resolution for all mentions. The experimental results show that the proposed model has a precision of 87.07%, recall of 65.39% and CoNLL F1 74.61%, which is an improvement of 21.83% compared to an existing rule-based model.