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Screening of the liver, serum, and urine of piglets fed zearalenone using a NMR-based metabolomic approach

  • Jeong, Jin Young;Kim, Min Seok;Jung, Hyun Jung;Kim, Min Ji;Lee, Hyun Jeong;Lee, Sung Dae
    • Korean Journal of Agricultural Science
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    • v.45 no.3
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    • pp.447-454
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    • 2018
  • Zearalenone (ZEN), a mycotoxin produced by Fusarium in food and feed, causes serious damage to the health of humans and livestock. Therefore, we compared the metabolomic profiles in the liver, serum, and urine of piglets fed a ZEN-contaminated diet using proton nuclear magnetic resonance ($^1H-NMR$) spectroscopy. The spectra from the three different samples, treated with ZEN concentrations of 0.8 mg/kg for 4 weeks, were aligned and identified using MATLAB. The aligned data were subjected to discriminating analysis using multivariate statistical analysis and a web server for metabolite set enrichment analysis. The ZEN-exposed groups were almost separated in the three different samples. Metabolic analysis showed that 28, 29, and 20 metabolites were profiled in the liver, serum, and urine, respectively. The discriminating analysis showed that the alanine, arginine, choline, and glucose concentrations were increased in the liver. Phenylalanine and tyrosine metabolites showed high concentrations in serum, whereas valine showed a low concentration. In addition, the formate levels were increased in the ZEN-treated urine. For the integrated analysis, glucose, lactate, taurine, glycine, alanine, glutamate, glutamine, and creatine from orthogonal partial least squares discriminant analysis (OPLS-DA) were potential compounds for the discriminating analysis. In conclusion, our findings suggest that potential biomarker compounds can provide a better understanding on how ZEN contaminated feed in swine affects the liver, serum, and urine.

Observation by the Microscopic Analysis of Lacquer Layer for Identification of Lacquer-ware Function (칠 기법 규명을 위한 칠도막의 현미경 관찰)

  • Kim, Soo-Chul;Lee, Kwang-Hee
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.6
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    • pp.96-104
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    • 2008
  • In this study, the observation of test pieces with an optical microscope was carried out after fabricating 4 groups of standard lacquer, in order to inquire into the ancient lacquering techniques. Group I had transparent reddish brown color, and the part of layer was indefinite, and the layer polished was easily distinguished. Group II had mostly transparent yellowish brown color, and it was possibility distinguished the layers when they were varnished with mixed lacquer after prime coating, and when they were varnished with lacquer as the prime coating. Group III set up membrane in the hardening process of the lacquer, and the lacquer and the unsaturated fatty acid of internal part was dried, so the top layer and the lacquer layer were separated and observed. Group IV: When the lacquer coat film of Group IV test pieces were observed in polarized light, the ferrous components were seen as the black and red color were mixed, and the silica crystals of silty soil were distinguished by reflection of lights. And the colors were distinguished as the lacquer layer of ruddle had red color, and the mixed lacquer of reddish lacquer and ruddle had the deep red color at the top and the bottom, but the middle part had the bright red color.

Effects of Cone Number and Thinning on Nutrient Content in Needles of Korean Pine (Pinus koraiensis)

  • Han, Sim-Hee;Kim, In-Sik;Kim, Hyun-Suk;Kim, Tae-Su;Jang, Kyung Hwan;Kim, Tae Dong
    • Journal of Korean Society of Forest Science
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    • v.97 no.5
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    • pp.516-524
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    • 2008
  • We investigated on the distribution of nutrient contents (N. P and K) in current and one-year old needles of Korean pine, Pinus koraiensis, to understand the physiological effects of alternate bearing. One experimental set was designed with three types of cone number (one, three and five) under natural state and cone number of another one was controlled by thinning. Test samples were separated to collect into three fractions; current needles on the shoot and one-year old needles above and under the cone. On the branch without cone, N, P and K concentrations in needles on the shoot were higher than those in needles of one-year old branch. At natural state, needle P concentration was the lowest in needles on the five-cone branch in August. Macronutrient concentrations were the lowest in needles above the cone, which was remarkably observed in needle K concentration. Under cone thinning, N. P and K concentrations were the highest in needles on one-cone branch and the lowest in needles on five-cone branch. Needle nutrient concentrations were the lowest in needles above the cone, which was remarkably observed in the needle on five-cone branch. In conclusion, the current needles of the shoot and cones are a strong sink of nutrient like N. P and K, and the increase of cone number influences nutrient removal from needles.

Cost-effective Investigation on Contaminated Land and Assessment of Measurement Uncertainty (오염토양의 효율적 조사기법과 측정불확도의 평가방법)

  • 이종천
    • Economic and Environmental Geology
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    • v.37 no.1
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    • pp.49-59
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    • 2004
  • The concectration and distribution of contaminants obtained from a contaminated land investigation or an environmental geochemistry survey constitutes the basis of a decision-making process on environmental policies or of scientific researches. As the quality of data determines the reliability of the result, the investigation plan should be adjusted according to the purpose of the investigation. In general, the effort to improve the data quality had been focused mainly on the QA/QC procedures in laboratories. The rapid progress of analytical instrument has also contributed toward improving the analytical precision to a sacrificable degree. Nevertheless, in many cases, it is not the analytical precision that needs improvement for the better precision of overall measurement process: it is rather during the sampling process in the field that is responsible for the poor precision. To assess the data quality on a measured value, ISO recommends to provide information on "measurement uncertainty" along with the measured value. The measurement uncertainty in an environmental measurement context can be explained as the statistical number that expresses the degree of the uncertainty stemming from the sampling and analytical procedures. There is a cost involved in order to improve the precision of sampling and analytical methods so as to decrease the degree of measurement uncertainty. The economical point of compromise in an investigation planning can be achieved when the allowable degree of uncertainty has been set before-hand. The investigation can then be planned accordingly not to exceed the uncertainty limit. Furthermore, if the measurement uncertainty estimated from the preliminary investigation can be separated into sampling and analytical uncertainties, it can be used as a criterion where the resources for the investigation should be allotted cost-effectively to reinforce the weakest link of the whole investigation process. This paper aims to present a method of estimating the level of measurement uncertainty of a measured contamination concentration at a site used as an example and to show how the estimated uncertainty can be applied to serve the particular purpose of an investigation.

Development of Computational Thinking-based Educational Program for SW Education (초등 SW교육을 위한 CT교육 프로그램 개발)

  • Ryu, Miyoung;Han, Seonkwan
    • Journal of The Korean Association of Information Education
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    • v.19 no.1
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    • pp.11-20
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    • 2015
  • The researches on the concept of justice and utilization for Computational Thinking with SW education are being actively discussed. However, a program has developed in conjunction with the actual elementary curriculum is not much. In this study, we have developed an educational program in applied mathematics based on CT. First, a separated view for a CT Application of mathematical concepts and objectives are set in three different application models. In order to achieve the CT-based math lessons, we also have developed a teaching and learning materials. We applied the developed materials in class, and to evaluate the satisfaction of learners. In addition to the validation of school application, we conducted a survey of professionals and teachers. The results of the analysis, the data showed that are helpful in the development of the student' CT ability as well as the ability to be helpful teaching and learning in school.

An Analysis of Critical Management Factors for Construction Failure on the Apartment Structural Framework using FMEA (FMEA 기법을 활용한 공동주택 골조공사의 건설실패 핵심관리요인 분석)

  • Oh, Chi-Don;Park, Chan-Sik
    • Korean Journal of Construction Engineering and Management
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    • v.13 no.3
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    • pp.78-88
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    • 2012
  • Previous construction failure researches were focused on the utilization plan based on failure information and development of failure classification. However, it has limitation to set up the plan for prevention of construction failure due to the lack of the number of on-site staffs. In order to prevent effectively construction failure, a prevention plan should be established through quantitative evaluation of failure causes. The purpose of this study is to suggest the assessment method for selection Critical Management Factor(CMF) and to analyze the CMF on the apartment structural framework using FMEA(Failure Mode and Effective Analysis) which is one of the methods of quantitative evaluation. The element of risk evaluation separated degree of failure risk and prevention respectively. The assessment method for selection of CMF can be utilized for planning proactive solutions on the failure, and it can be also selected critical factors about each project phases, type of facility and construction work.

Experimental Study on Cement Cohesion Reduction Effect of Grout Mixer with Vibration Filter (진동필터가 설치된 그라우트 믹서의 시멘트 응집 저감 효과에 대한 실험적 연구)

  • Hwang, Sung-Pil;Jeoung, Jae-Hyeung;Kim, Chang-Yong;Lee, Woo-Je
    • The Journal of Engineering Geology
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    • v.28 no.1
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    • pp.61-67
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    • 2018
  • Grouting is reinforcement or cutoff method which uses the hardening agent which is typically represented by portland cement and injected into the ground or the structure. When mixing the cement in powder form with water, the particles tend to cohere each other. Once they cohered, the particle size tends to become larger while injection efficiency becomes lower. This study, in a bid to reduce the cohesion of cement, the screen was set inside the grout mixer so that the cement particles are separated while vibrating them. To validate the effect of vibration screen, comparison test was conducted by using ordinary portland cement, slag cement and micro cement. Viscosity test, bleeding test and grain-size analysis indicated that the characteristics varied significantly after passing through the vibration filter. It is expected that the vibration filter installed inside the grout mixer will reduce the cement cohesion when mixing with water.

A Historical Study on the Achitectural Cooking and Storing Spaces in Traditional Korean Houses (한국(韓國)의 전통적(傳統的) 식생활공간(食生活空間))

  • Joo, Nam-Chull
    • Journal of the Korean Society of Food Culture
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    • v.2 no.2
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    • pp.169-179
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    • 1987
  • In the life style of the neolithic age, cooking and sleeping space was in one room dugout without differentiation of spaces, so to say one room system. Ro(a kind of primitive fire place) was used for both cooking and heating. However, in the early part of the Iron Age, the uses of Ro were separated into two major uses of cooking and heating. Especially, L-shaped Kudle(an unique under floor heating structure of Korea) was invented for the new system of heating, extending to Koguryo Period. The life style of Koguryo Dynasty could be seen through the mural paintings of tombs. For these mural paintings contain of cooking space(Kitchen), meat storage, and mill house drawing, we can recognize that houses were specialized many quaters according to their function. Also a kitchen fuel hole for preparing food was built without relation to L-shaped Kudle. But during Koryo Period, Kudle could be set up all over the room, the so-called Ondol(the unique Korean panel heating system) settled down. From this development of heating system, room could be adjacent to kitchen, and kitchen fuel hole and heating fuel hole be onething. This system was developed with variety, extending to Chosun Dynasty. In the period of Chosun Dynasty, a kitchen was made close to an Anbang(Woman's living room), and Anbangs Ondol was heated by the warmth of a cooking fireplace. Therefore Handae Puok, outer kitchen was used in summer. As for its storage space, it was seen that there were a pantry near the kitchen and a store house constructed as an independant building. In the latter, it was devided into a firewood storage, a Kimchi storage, and a rice storage, etc. Especially it is a unique feature that 'Handae-Duyju', an outer rice chest which keeps rice, was constructed as an isolated small building.

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Simultaneous Determination of Six Bioactive Components in Guibi-tang by HPLC-DAD (HPLC-DAD를 이용한 귀비탕 중 6종 생리활성 물질의 동시분석법 확립)

  • Yang, Hye-Jin;Weon, Jin-Bae;Ma, Jin-Yeul;Ma, Choong-Je
    • Korean Journal of Pharmacognosy
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    • v.41 no.4
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    • pp.313-318
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    • 2010
  • Guibi-tang, a traditional herbal medicine, is used for anti-oxidant, anti-osteoporosis, hemostasis and gastroprotection. To develop an analysis method of simultaneous determination of six compounds, swertisin, decursinol, glycyrrhizin, 6-gingerol, costunolide and decursin in Guibi-tang, a high performance liquid chromatography was used with diode array detector. Six bioactive components were separated on a SHISEIDO $C_{18}$ column ($5\;{\mu}m$, 4.6 mm I.D.${\times}$250 mm) with column temperature $30^{\circ}C$. The gradient elution was composed of water with 0.1% trifluoroacetic acid (TFA) and acetonitrile. UV wavelength was set at 230 nm, 254 nm and 330 nm, respectively. Calibration curve showed good linear regression ($R^2$ > 0.9999). The limits of detection (LOD) and the limits of quantification (LOQ) ranged in 0.03 - 0.23 ${\mu}g/ml$ and 0.08 - $0.70\;{\mu}g/ml$, respectively. The RSD values of intra- and inter-day test were in the range of 0.03 ~ 0.96% and 0.01 ~ 1.46%, respectively. The evaluated results of accuracy test were varied from 92.28% ~ 105.14% with RSD < 1.60%. In conclusion, this developed simultaneous determination method was accuracy and sensitive to the quality evaluation of Guibi-tang.

A Study on Thermo-Bed Design Development of HPT System (HPT 방식의 온열침대 디자인 개발에 관한 연구)

  • Lee, Bong-Kyu
    • Journal of the Korea Furniture Society
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    • v.22 no.1
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    • pp.72-81
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    • 2011
  • These days, beds have been developed not as furniture to sleep in but Healthcare Furniture. The function of heating, non-electronic-waves and far-infrared rays have been added. Also, eco-friendly materials such as stone, mud, rubber and wood are combined to them together with the radiation-heating method using heat conduction through electronic coil and hot water circulation. Modern people suffer from stress and fatigue. The hot issue is "health." Focused on the health, the development of design for beds considering the influence electronic waves, anions, deep sleep and the density of toxic materials have on human bodies has become urgent. In this study, the trend, specification and efficiency of the functional health-related electronic coil technology are analyzed. Through the analysis, the environmental standards are set. According to them, technology, eco-friendly materials and additional functions for the development of the design are considered. Also, the government is promoting the IEC international standardization for the Korean traditional floor heating style beds. In preparation for that, we adopted new HPT method technology. It enables non-electronic-waves, low electricity and stable temperature maintenance control for the warm top and the cool bottom. Also, the head boards and the frames of the beds can be separated. Through this separation, the beds can be easily moved or installed. Eco-friendly materials such as Hinoki cypress wood and red clay and the existing tables function are combined to the head boards so they can be used as drawers and display shelves. If they are used as separate items, they can be used as covers. This is how we suggest the design for the heating beds.

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