• Title/Summary/Keyword: separated fractions

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Electrophoretic Patterns of Hemolymph Protein of Healthy and Nuclear-Polyhedrosis Virus Infected Silkworm, Bombyx mori. (건강잠 및 핵다각체 이병잠의 체액단백질의 전기영동상)

  • 김낙정;강석권;김정일
    • Journal of Sericultural and Entomological Science
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    • v.13 no.2
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    • pp.141-143
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    • 1971
  • By means of thin-layer electrophoresis in agarose gel, hemolymph protein of healthy silkworm larvae and of the nuclear polygedrosis virus infected larvae were studied. 1. In the 4th instar, 4 fractions moving toward anode were separated. Dye-binding Capacity of the fraction was increased according to the stage. 2. After 5th day in the 5th instar, 7 fractions moving toward anode were separated, and one fraction toward cathode was separated. 3. On the first day in the 5th instar, 5 fractions were separated, and on the 4th day of the same instar 5 fractions were separated. 4. As for the hemolymph protein fractions of the polyhedrosis virus infected larvae, on the 6th and 7th day, three fractions(D.E.F) were inclined to increase, whereas on the 8th day 4 fractions(A.B.D.E) were disappeared but F fraction was inclined to decrease.

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Antimicrobial and Antitumor Activity of Triterpenoids Fraction from Poria cocos Wolf (재배 복령(Poria cocos Wolf)의 Triterpenoids 분획의 항균 활성 및 항암 활성)

  • 정신교;권미선;최종옥;송경식;이인선
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.28 no.5
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    • pp.1029-1033
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    • 1999
  • Seven fractions were separated by silica gel chromatography from the diethyl ether souble portion of the methanolic extract of the cultured hoelen(Poria cocos Wolf). Three fractions were separated from the Fr.II and Fr.IV by rechromatography, respectively. The most active fraction, Fr.II 3 and Fr.IV 3, were separated to 2 and 4 fractions by preparative HPLC. On the result of antimicrobial test, triterpenoids fractions showed weaker effect than benzoic acid but Fr.II 3 1, Fr.II 3 2 had an excellent antimi crobial activity. Triterpenoids fraction of hoelen(Poria cocos Wolf) showed a high inhibition activity on the growth of lung cancer, ovary cancer, skin cancer, central nerve cancer and rectum cancer cell, especially the activity of Fr.II 3 1 and Fr.II 3 2 was the highest.

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$S^{35}$(1,2-dichloro vinyl) L-Cysteine의 소에 의(依)한 대사물(代謝物)의 분리(分離)

  • Kim, Jae-Uk
    • Applied Biological Chemistry
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    • v.2
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    • pp.36-39
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    • 1961
  • It has been ascertained that S-(1, 2-dichlorovinyl) L-cysteine (DCVC) is probably the toxic component in the trichloro-ethylene extracted soybean oil meal on the bovine. For the study of the metabolites of DCVC, the components with radioactive $S^{35}$-in the urine of the calf, to which $S^{35}$-DCVC was administrated, were separated using of cellulose powder with propanol-water (70:30 V/V) which is easily removable by evaporation. As the results followings were obtained: Peak 1, which shows fractions containing single $S^{35}$-radioactive components, whose Rf is 0.815 Peak 2, which shows fractions containing jingle $S^{35}$-radioactive component, whose Rf is 0.447 Peak 3, which shows fractions containing both $S^{35}$-radioactive components whose Rfs are 0.090 and 0.171 Peak 4, which shows fractions containing single $S^{35}$-radioactive component, whose Rf is 0.142. Peak 5, which shows fractions containing single $S^{35}$-radioactive component, whose Rf is 0.084. Besides these, two of small peak were obtained. Although, the resolving power of the cellulose powder column is not sufficient, it is applicable for the study of the components of metabolytes of DCVC conveniently with the rest of removable solvent easily. Also the components with radioactive $S^{35}$ in the feces of the calf were separated using citrate buffer gradient system of Moore and stein. As the results; three $S^{35}$-radioactive components were separated.

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Studies on the volatile compounds of Cnidium officinale (천궁(Cnidium officinale)의 향기성분)

  • 이재곤;권영주;장희진;김옥찬;박준영
    • Journal of the Korean Society of Tobacco Science
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    • v.16 no.1
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    • pp.20-25
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    • 1994
  • The volatile components were extracted from root of Cnidium officinale M. by SDE(Simultaneous steam distillation and extraction) apparatus and analyzed by GC/M.5 and GC retention index matching. The experimental results revealed the presence of over 22 volatile components. Major components were cnidilide (35.1%), neocnidilids (13.4%), ligustilide (23.2%). The essential oils were separated by silica gel column chromatography(Merck 70-230mesh), and 4 fractions among 12 fractions separated had a, good aroma character.

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Effect of Ganoderma lucidum Mycelial Fractions on the Functional Recovery of Primary Cultured Hepatocytes. (영지 균사체 분획이 일차배양 간세포 기능회복에 미치는 영향)

  • 박혜선;현진원;김하원;심미자;김병각
    • Microbiology and Biotechnology Letters
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    • v.28 no.4
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    • pp.209-213
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    • 2000
  • The cultured mycelia of Ganoderma lucidum were extracted and the extract was separated into six fractions by organic solvent fractionation. The antihepatotoxic activity of all the fractions was evaluated by measuring activities of glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT). Among the fractions tested, the high-polarity fractions such as aqueous and n-butanol fractions significantly reduced activities of GPT and GOT in CCl4- and galactosamine-intoxicated rat primary hepatocytes. When intracellular synthetic activities were measured by pulsing the rate primary cultured hepatocytes with [3H]-uridine and [3H]-leucine, activities of DNA, RNA and protein. When direct toxicities of the fractions were measured against human hepatoma(SK-Hep-1), the non-polarity fractions such as n-butanol and ethyl acetate fractions showed potent direct cytotoxicities even at the concentration of 1 $\mu\textrm{g}$/ml. These data showed that Ganoderma lucidum has hepatoprotective and hepatotoxic recovery principles in its mycelia.

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Volatile flavor components of Jindalrae flower(Korean azalea flower, Rhododendron mucronulatum Turczaninow) (진달래꽃의 휘발성 성분에 관한 연구)

  • Chung, Tae-Yung;Lee, Seung-Eun
    • Applied Biological Chemistry
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    • v.34 no.4
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    • pp.344-352
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    • 1991
  • The whole volatile flavor concentrate obtained from Jindalrae flower was separated into hydrocarbon and oxygen-containing compound(OCC) fractions, and the OCC-fraction was further separated by column chromatography into nine sub-fractions, respectively. These fractions were analyzed by gas chromatography and combined gas chromatography/mass spectroscopy. One hundred and sixty-two components, including 61 hydrocarbons, 18 aldehydes, 18 esters, 41 alcohols, 3 ketones, 4 oxides, 8 acids, 6 phenols and 3 miscellaneous components, were identified.

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Content and Distribution of Transition Metals and Rare Earth Elements in Magnetically and Mechanically Separated Brown Coal Ash

  • Malikov, Sh.R.;Pikul, V.P.;Mukhamedshina, N.M.;Sandalov, V.N.;Kudiratov, S.;Ibragimova, E.M.
    • Journal of Magnetics
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    • v.18 no.3
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    • pp.365-369
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    • 2013
  • Coal ash is known to contain a noticeable amount of valuable elements, including transition metals and lanthanides. Therefore it is quite actual problem to extract them for metallurgy and other applications. This paper presents the results of high gradient magnetic and mechanical separation, microscopy, element analyses and optical spectroscopy of brown coal ash taken from the combustion camera and chimney-stalk of Angren thermal power station. The separated magnetic fraction was 3.4 wt.%, where the content of Fe in ferrospheres increased to 58 wt.%. The highest contents of Fe and rare earth elements were found in the fine fractions of $50-100{\mu}m$. Optical absorption spectroscopy of water solutions of the magnetic fractions revealed $Fe^{2+}$ and $Fe^{3+}$ ions in the ratio of ~1:1. The separated coal ash could be used for cleaning of technological liquid waste by means of the high gradient magnetic field.

Effects of Serum Fractions Separated by Molecular Weight on the Development of Mouse Embryos Fertilized In Vitro (분자량에 따라 분획화된 혈청성분이 생쥐 체외수정란의 발생에 미치는 영향)

  • 한정호;정구민
    • Journal of Embryo Transfer
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    • v.9 no.1
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    • pp.127-137
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    • 1994
  • This study was carried out to investigate the inhibiting or promoting effect of fetal bovine serum fractionated by the molecular weight and to examine the effect of reconstruction of serum fractions on the development of 1- and 2-cell mouse embryos fertilized in vitro (IVEE) . The serum was separated by ultrafiltration or gel filtration methods and added in m-KRB medium for culture of IVFE. The developemental ability(cavitation and hatching) of embryos following culture of day 4 and 6 was compared among fractions. Small molecular weight fraction( <3 kDa) significantly inhibited the development of 1-and 2-cell IVFE to the blastocyst stages, compared with other fractions. One-cell IVFE were more sensitively damaged than 2-cell embryos by that fraction and arrested mainly at 2~4 cell stages. Moreover, small amount(<3%,v /v) of the inhibiting fraction acted even with protein rich fraction(100~30 kDa) and arrested the embryonic development. On the other hand, 100~30 kDa fraction promoted the embryonic development and no inhibiting effect was observed at the level of 50%(v /v) in culture medium In the experiment of gel filtraton, =30 kDa fraction showed the highest promoting effect on the embryonic development, but <4 kDa fraction inhibited significantly the development. These results suggest that serum contains not only small molecular weight inhibitory component(s) but also promoting one rather than albumin on embryonic development. And serum can be more effectively used in the IVF program after removal of inhibitory component(s) by one of above separation methods.

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Separation of Ether-Soluble Neutral Extract of a Commercial High-Grain Feed Stimulating Hay Intake in Cattle

  • Shahjalal, M.;Dohi, H.;Kosako, T.;Hayashi, Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.2
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    • pp.188-191
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    • 2000
  • Appetite stimulant ether-soluble neutral extract of a high-grain compound feed was separated into four fractions with an open column of silica gel chromatograph using four carrier solutions containing n-pentane and diethyl ether as 100:0 (Fraction A), 90:10 (Fraction B), 75:25 (Fraction C) and 0:100 (Fraction D). The stimulative effects of the fractions were examined by comparing the intake of hay (with or without the fractions) in two-choice test bioassays with cattle. The Fractions A, B and C of the neutral extract stimulated (p<0.05) hay consumption in cattle, whereas Fraction D had no effect on selective feeding when compared with the control fraction. Furthermore, Fractions A, B and C had higher (p<0.05) feeding stimulative indices (FSI) than that of the Fraction D. The results suggest that chemical stimulants to increase palatability of hay are present in the neutral Fractions A, B and C of high-grain concentrate.

Isolation of Antioxidative Components of Perillae semen (자소자 항산화성분의 분리)

  • Kim, Yong-Jae;Kim, Choong-Ki;Kwon, Yong-Ju
    • Korean Journal of Food Science and Technology
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    • v.29 no.1
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    • pp.38-43
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    • 1997
  • Free phenolic acids (FPA), soluble phenolic acid esters (SPA) and insoluble-bound phenolic acids (IPA) were extracted from defatted Perillae semen flour and the antioxidative components in FPA extract was separated by column chromatography and HPLC. Total phenolic content of defatted Perillae semen flour was 0.38% as chlorogenic acid and each percent ratio of the content of FPA, SPA and IPA to total phenolic content was 71.1%, 15.8% and 13.1%, respectively. The antioxidative activity was compared by measuring of electron donating ability (EDA) and thiobarbituric acid value (linoleic acid substrates). The FPA extract was showed the highest antioxidative activity among the three kinds of phenolic extracts. The FPA extract showing the highest antioxidative activity was separated by silica gel column chromatography and then the separated fractions were compared in terms of antioxidative activity. The fractions of acetone : methanol (8 : 2) showing the highest antioxidative activity was further separated by HPLC. Five fractions (F-I, F-II, F-III, F-IV and F-V) were observed on the HPLC chromatogram and F-I fraction showed the highest antioxidative activity.

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