• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.310-310
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• 2011

Morphological evolution of amorphous carbon film is investigated by molecular dynamics simulation. Here, energetic carbon atoms (75 eV) are deposited on the diamond (001) substrate to find effect of incidence angles. At normal and near-normal incidences ($0^{\circ}{\sim}30^{\circ}$) atomically smooth surfaces are observed during their growth. However, rough surfaces emerge and develop into a ripple structure at grazing incidences ($60^{\circ}{\sim}70^{\circ}$). The different growth modes according to the incidence angles can be described by impact-induced displacements of atoms. Downhill transport along any sloped surfaces is predominant for the case of normal incidence. As the incidence angles become grazing, uphill transport is allowed along the surfaces, which have smaller slopes than incidence angle, so the surface features can be amplified. Impact-induced transport and self-shadowing effect can be responsible to the initial growth of seeding structures at a grazing incidence, which would be grown up as tilted columnar structures in further depositions.

• Journal of Biosystems Engineering
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• v.3 no.2
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• pp.22-33
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• 1978

To maintain the self-sufficiency of food production , it is required to improved the productivity and increase the effective utilization of land in the double cropping areas using improved cultivation technology. The following reasons encourage this cours of action because the overlapping rice harvest and barely seeding seasons complete for labour, the lower price of barley makes it financially less attractive to grow and these two facts together are responsible for a decrease in barley being planted in certain more seasonably marginal double cropping areas. Fro these reasons, it is desirable to carry out tests to improve the current cultivation techniques . In this interest , the following studies were carried out in the experimental field located at Banweol-myeon, Whasung-jkun, Gyeonggi-do from October 1977 to July 1978 in order to establish the mechanical method of barley cultivation using the power tiller. The summarized results are as follows. 1. The work performance in the seeding operation using the rotary barley seeder was 68 minutes per 10 ares which compares favourably with 408 min/10a using the conventional method. This is only one sixth of the time required by th conventional method. The operating costs using the rotary barley seeder was 1, 463 won per 10 areas as against 3, 486 Won per 10 ares for the conventional method, showing a saving of about 50% in comparison with the conventional method. 2. In the manure spreading operation, the work performance was 25 min/10a for the manure spreading machine , compared to 1089 min/10a for the manual operations. This is about one fourth of the time required by the conventional method, The operating costs were 810 Won/10a for the machine and 857 Won/10a for the manual labour. This cost shows little advantage by the machine over the manual labor costs. The conventional method of manure spreading will continue pending decision which will develope and improve the machine.3. Work performance in the rolling operation using the barley rolIer was 30 min/lOa which compares favourably with 135 min/10a using conventional method. This was one fourth of the time required by the conventional method. The operating costs were 514 Won/103. for the machine and 1, 003 Won/lOa for manual labour. In the weed control operation, the work performance and operating costs were 45min/lOa, 1, 399won/10a for the herbcide application using the power sprayer, 1, 149min/10a 8, 541won/lOa for the conventional method respectively. This is 26 times higher efficiency in comparison to the conventional method. 4. In the harvesting operation using the reaper binder and tiller attachment, the work performance was 60min/lOa and represents a cost of 2, 039won/10a. The con\ulcornerventional method took 640min/10a at a cost of 4, 757won/lOa. The reaper binder showns a saving of one tenth over the conventional method. The automatic thresher is already recommended for the current situations, and is now being used. 5. From a comparison of the results of the above trials, the serial cultivation system using the attachment for the power tiller such as the rotary barley seeder is now to be recommended for the current barley cultivation system. It is also recommend from these results that the mechanized technology now available must be used to improve and maintain the increase in barley production. It is seen th1t this is the only course now avaihble to solve the peak seasonal requirements of labour needed for l11rvesting and seeding between rice and barley cultivation.

• Journal of Microbiology and Biotechnology
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• v.17 no.12
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• pp.2027-2032
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• 2007

[ ${\alpha}$ ]-Synuclein is the major component of Lewy bodies and responsible for the amyloid deposits observed in Parkinson's disease. Ordered filamentous aggregate formation of the natively unfolded ${\alpha}$-synuclein was investigated in vitro with the periodic ultrasonication. The ultrasonication induced the fibrillation of ${\alpha}$-synuclein, as the random structure gradually converted into a ${\beta}$-sheet structure. The resulting fibrils obtained at the stationary phase appeared heterogeneous in their size distribution, with the average length and height of $0.28\;{\mu}m{\pm}0.21\;{\mu}m$ and $5.6\;nm{\pm}1.9\;nm$, respectively. After additional extensive ultrasonication in the absence of monomeric ${\alpha}$-synuclein, the equilibrium between the fibril formation and its breakdown shifted to the disintegration of the preexisting fibrils. The resulting fragments served as nucleation centers for the subsequent seed-dependent accelerated fibrillation under a quiescent incubation condition. This self-seeding amplification process depended on the seed formation and subsequent alterations in their properties by the ultrasonication to a state that accretes the monomeric soluble protein more effectively than their reassociation of the seeds back to the original fibrils. Since many neurodegenerative disorders have been considered to be propagated via the seed-dependent amyloidosis, this study would provide a novel aspect of the significance of the seed structure and its properties leading to the acce]erated amyloid formation.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.225-229
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• 2016

This study was conducted to investigate the herbicidal efficacy of up granule formula of benzobicyclon mixtures, benzobicyclon + azimsulfuron + oxaziclomefone up granule (BAO UG) and benzobicyclon + bensulfuron-methyl + fentrazamide up granule (BBF UG) to control annual and perennial weed species in a rice paddy field. Four annual weed species including Echinochloa crus-galli L. and three perennial weeds were dominated in the experimental field. Application of BAO UG and BBF UG was highly effective to control both annual and perennial weed species. No phytotoxic effect was observed in the rice based on the plant height, the number of tillers, and culm and panicle length. Finally, rice yield in the BAO UG and BBF UG treatment was as much as that in the hand weeding. This study indicates that self-dispersible floating formula of benzobicyclon mixtures can be applied to control both annual and perennial weed species in rice paddy fields and contribute to save labor costs.

• Korean Journal of Veterinary Research
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• v.58 no.4
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• pp.201-209
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• 2018

Canine mammary tumors are among the most frequently observed cutaneous tumors in female dogs. Cancer stem cells (CSCs), referred to as tumor-initiating cells, are thought to have properties similar to normal stem cells such as the ability to self-renewal and to differentiate into various cell types. Biological understanding of CSCs and the critical pathways involved in their maintenance are important in research and therapy for mammary tumors. We conducted the present study on sphere formation from REM134 cells by using methylcellulose to produce tumorspheres on a large scale and compared the specific markers of the spheres-formed and plating-cultured REM134 cells. The results revealed that the tumorspheres cultured in methylcellulose had higher seeding density and improved morphology compared to those produced in normal sphere formation medium. Expression levels of stemness markers and CSC-related markers were higher in tumorsphere-forming cells than in plating-cultured cells. Subsequently, we transplanted the tumorsphere-forming and plating-cultured cells into female nude mice to examine their tumorigenic potential. Tumor volume increased rapidly in mice transplanted with tumorsphere-derived cells compared to plating-cultured cells. We observed a novel sphere-forming condition for REM134 cells and showed that REM134 cell tumorspheres can exhibit improved CSC properties.

• Korean Chemical Engineering Research
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• v.57 no.5
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• pp.606-610
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• 2019

In this study, we demonstrated that the nonenzymatic glucose sensor based on the flexible carbon fiber bundle electrode with BDD nanocomposites (CF-BDD electrode). As a nano seeding method for the deposition of BDD on flexible carbon fiber, electrostatic self-assembly technique was employed. Surface morphology of BDD coated carbon fiber electrode was observed by scanning electron microscopy. And the electrochemical characteristics were investigated by cyclic voltammetry, electrochemical impedance spectroscopy and chronoamperometry. This CF-BDD electrode exhibited a large surface area, a direct electron transfer between the redox species and the electrode surface and a high catalytic activity, resulting in a wider linear range (3.75~50 mM), a faster response time (within 3 s) and a higher sensitivity (388.8 nA/mM) in comparison to a bare CF electrode. As a durable and flexible electrochemical sensing electrode, this brand new CF-BDD scheme has promising advantages on various electrochemical and wearable sensor applications.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.495-500
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• 2001

Seed germination, seedling growth, plant establishment after transplanting, set of female flowers, and subsequent fruit growth were examined in cucumbers grown in soils having varying electrical conductivities (EC) of $1.0-5.0dS{\cdot}m^{-1}$ adjusted by adding different amount of a commercial compound fertilizer (N : $P_2O_5$ : $K_2O$ = 21 : 17 : 17). Slower seed germination was recorded in soils having EC value of $3.0dS{\cdot}m^{-1}$ or higher and little or no germination took place in soils with EC $4.0dS{\cdot}m^{-1}$ until 8 days after sowing. Suppression of seedling growth was apparent in soils having EC level of $2.5dS{\cdot}m^{-1}$. All seedlings died eventually at EC level of $3.5dS{\cdot}m^{-1}$. Leaf color became progressively dark green with the increase in EC from 1.0 to $3.0dS{\cdot}m^{-1}$. The first female flower was formed at higher node and the number of days required for the first flowering of female flower increased with the increase in EC values up to $3.0dS{\cdot}m^{-1}$. Occasionally, leaf yellowing and self-topping symptoms appeared in plants grown in soils having EC level of $3.0dS{\cdot}m^{-1}$.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.1
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• pp.100-113
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• 2007

In Korea, there has been no revegetation applicable standard for slopes formed by road construction work up to now, so revegetation work has been done using mostly foreign varieties in the manner of early revegetation. However, foreign varieties have some shortcomings; for example, they tend to be dried to death after construction work. Besides, due to the rift in the supporting soil, these revegetated varieties are often displaced from the slopes. Thus, the Ministry of Construction & Transportation on July, 2005 established revegetation standard on the slopes suited for the natural eco-system of Korea and organized positively recommending directions for using self-sewn plants growing near the slopes in overall consideration of soil, weather, regional conditions. The locations specified in this direction are the slopes at the road construction jobsite like the expressway, highways, and local roads. In addition, the Ministry's standard and directions stipulated that damaged slopes's natural environment and eco-system due to various road construction work should be restored, and thus a feeling of stability and pleasantness should be provided to road users as well. Also, the Ministry tried to select seed plants and revegetation measures suited for surrounding environment to put the environment-friendly slope revegetation measures into practice through the test revegetation work in order to prevent illegal construction practices and to improve the quality of revegetation. According to the direction, revegetation districts aimed at the slopes are divided into three ones in consideration of weather environment, regional environment, and forest environment as follows : national territory's core ecological green-land based district centering on the Taebaek Mountains; coastal ecosystem district including islands off the coast; inland eco-system district. The combination of revegetation plants according to environment revegetation districts, should be executed by dividing into herb-oriented type, woody plant colony type, and bio-species versatility restoration type, and the selection of seeding plants should be done in the presence of a supervisor and through test construction results and technology counseling from a specialist in natural eco-system restoration and revegetation measure seed combination standard according to environment revegetation districts. This direction will be executed in the manner of monitoring until the yea 2008 and 2009 it will be finalized and enforced on December, 2009.

• Korean Journal of Medicinal Crop Science
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• v.1 no.1
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• pp.74-80
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• 1993

Survey on the status of cultivation and production of medicinal crop was studied on 18 medicinal herbs and 5 medicinal trees in 66 major crop producing complexes. Native seeds or seedlings of the crops obtained from self-seed or neighbored farmer were cultivated and organic fertilizer was preferred to chemical fertilizer in the complexes. More than 60.7% of farmers were applied compost among the organic fertilizers, while 34.8% of them cultivated without any organic fertilizers. Both of using the compound chemical fertilizers and urea and urea only used were showed 51. 5% and 40.9%, respectively. Ostericum koreanum, Angelica gigas, Phellopterus littoralis, and Bupleurum fulcatum belonging to the family Umbeliferae and Aralia continentalis, Polygonatum sibiricum and Eucommia ulmoides in the complexex were not damaged by insect pests and plant diseases. Mite, aphid, grub, cutworm, onion magot fly and european corn borer were known to as major insect pests and anthracnose, powdery mildew, brown leaf spot, leaf blight and root rot as major plant diseases. Productivity and income of a farm by medicinal crops in the complex were greatly different from seeding time, planting distance, year yielded, and selling routes even in the same cultivation method and in the same cultivated area.

• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.215-220
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• 2007

This study was conducted to examine the establishment of bovine ES-like cells having pluripotency. The hatched blastocysts derived from culture of in vitro fertilized embryos for 10 to 12 days dissociated mechanically into ICM-and trophectoderm-rich clumps using needle, and cultured onto mitotically-inactivated MEF feeder layer. The primary colonies originated from ICM cells were detached mechanically 7 days after seeding and subsequent subculture was conducted at intervals of every 5 to 7 days. Two ES -like cell lines were established and maintained over 40 passages. Self-renewal of the established lines was confirmed by examining the alkaline phosphatase activity, stem cell-specific marker profiles including SSEA isotopes, Oct-4 and STAT3. Moreover, the established cell lines could produce anchorage-independent embryoid bodies (EBs) with gradual decrease of Oct-4 transcript level in time-dependent manner.