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Establishment and Maintenance of Embryonic Stem-like Cell Lines from In Vitro Produced Bovine Blastocysts  

Lee, Yu-Yeon (Center for Regenerative Medicine Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Kim, Sun-Uk (Disease Model research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Kim, Ji-Su (Center for Regenerative Medicine Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Song, Bong-Seok (Center for Regenerative Medicine Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Cho, Yoon-Jeong (Center for Regenerative Medicine Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Park, Jung-Sun (Center for Regenerative Medicine Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Yu, Dae-Yeul (Disease Model research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Jin, Dong-Il (Division of Animal Science and Resources, Research Center for Transgenic and Cloned Pigs, Chungnam National University)
Lee, Kyung-Kwang (Center for Regenerative Medicine Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Koo, Deog-Bon (Center for Regenerative Medicine Korea Research Institute of Bioscience and Biotechnology(KRIBB))
Publication Information
Reproductive and Developmental Biology / v.31, no.3, 2007 , pp. 215-220 More about this Journal
Abstract
This study was conducted to examine the establishment of bovine ES-like cells having pluripotency. The hatched blastocysts derived from culture of in vitro fertilized embryos for 10 to 12 days dissociated mechanically into ICM-and trophectoderm-rich clumps using needle, and cultured onto mitotically-inactivated MEF feeder layer. The primary colonies originated from ICM cells were detached mechanically 7 days after seeding and subsequent subculture was conducted at intervals of every 5 to 7 days. Two ES -like cell lines were established and maintained over 40 passages. Self-renewal of the established lines was confirmed by examining the alkaline phosphatase activity, stem cell-specific marker profiles including SSEA isotopes, Oct-4 and STAT3. Moreover, the established cell lines could produce anchorage-independent embryoid bodies (EBs) with gradual decrease of Oct-4 transcript level in time-dependent manner.
Keywords
Embryonic stem cell; Whole blastocyst; Bovine; In vitro;
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