• Title/Summary/Keyword: seedling method

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Resistance of Terpenoids to Various Abiotic Stresses in Chamaecyparis obtusa

  • Min, Ji Yun;Park, Dong Jin;Yong, Seong Hyeon;Yang, Woo Hyeong;Seol, Yuwon;Choi, Eunji;Kim, Hak Gon;Choi, Myung Suk
    • Journal of agriculture & life science
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    • v.53 no.3
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    • pp.17-26
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    • 2019
  • Chamaecyparis obtusa is one of the economical conifers planted in Korea due to its good quality timber and wood characteristics. Individuals of C. obtusa containing high terpenes (HT) and low terpenes (LT) were selected for by colorimetric method. The HT of C. obtusa was delayed in wilting against various abiotic stresses compared to the LT plants. The HT group exposed to UV did not significant influence the chlorophyll content, and the chlorophyll value was higher in the HT group than the LT group. Also, chilling treatment (5℃) did not significant influence on the chlorophyll content. However treatment at -4℃ showed relatively low chlorophyll content in the LT group than the HT group. Plants exposure to high temperature was not a difference between the HT and the LT group. However, treatment at 38℃ influenced the chlorophyll content that was increased exposure time-dependently. In salt treatments, chlorophyll in the HT group was lower at high concentrations (300 and 500 mM) of NaCl. However, chlorophyll content increased to slightly in treatment time-dependently, which is 6.7% to 40%. H2O2 treatment has been a negative effect on the chlorophyll content in the HT group. All concentration of H2O2 decreased the chlorophyll content of 5% to 35%. Plants containing high terpenoids were resisted against some abiotic stress such as salt and H2O2. Our results implied that terpenoids could cause various abiotic stress resistance. These results could be utilized for efficient management and biomass production during forest silvicultures.

The Effects of 7 Fertilizers on the Growth and Nutrient Concentrations of Fraxinus rhynchophylla, Fraxinus mandshurica, Pinus koraiensis, and Abies holophylla Seedlings (7가지 시비처리가 물푸레나무, 들메나무, 잣나무, 전나무 묘목의 생장 및 양분농도에 미치는 영향)

  • Han, Si Ho;Byun, Jae Kyung;Cho, Min Seok;An, Ji Young;Park, Gwan Soo;Kim, Se Bin;Park, Byung Bae
    • Journal of Korean Society of Forest Science
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    • v.105 no.2
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    • pp.177-185
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    • 2016
  • Fertilization is required to keep the balance of tissue nutrients and to produce high quality seedlings at the permanent nursery. This study was conducted to verify the optimum fertilization method for Fraxinus rhynchophylla, F. mandshurica, Pinus koraiensis, and Abies holophylla seedlings with vector diagnosis method. Seven treatments include nitrogen (N, $13.8g{\cdot}m^{-2}$), phosphorus (P, $6.1g{\cdot}m^{-2}$), potassium ($7.5g{\cdot}m^{-2}$) fertilization and 1x (N $6.9g{\cdot}m^{-2}$, P $3.05g{\cdot}m^{-2}$, K $3.65g{\cdot}m^{-2}$), 2x (twice of 1x), 4x (four times of 1x) fertilization and no fertilization. Soil pH decreased as fertilization increased. Nitrogen and NPK fertilization decreased exchangeable $Ca^{2+}$ and $Mg^{2+}$ concentrations. Height and root collar diameter of F. rhynchophylla and F. mandshurica significantly increased with N and NPK fertilization, but those of P. koraiensis and A. holophylla did not. The biomass of F. rhynchophylla and F. mandshurica was about twice higher at NPK fertilization compared to the control. The responses of vector diagnosis were different by tree species and fertilization treatment: F. rhynchophylla was in the status of N "dilution", which means the N concentration decreases with N content. Phosphorus and K were "sufficiency" state with 4x fertilization. F. mandshurica showed "retranslocation" as N content decreased without change of dry weight at N, P, K fertilization, but "dilution" state at NPK fertilization. This result suggested that optimal fertilization was required for F. rhynchophylla and F. mandshurica in seedling production stage, but was not essential for P. koraiensis and A. holophylla.

The Effect of Polypropylene Mulching Method on Growth of Quercus glauca Thunb. Seedling and Weed Treatments (부직포 멀칭 방식에 따른 종가시나무 묘목의 생장과 제초에 미치는 영향)

  • Sung, Chang-Hyun;Yoon, Jun-Hyuck;Jin, Eon-Ju;Bae, Eun-Ji
    • Journal of the Korean Institute of Landscape Architecture
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    • v.48 no.5
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    • pp.59-66
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    • 2020
  • Recently, cultivation and management technologies have been needed to adapt due to climate change, which is causing abnormal weather conditions. One technique is to increase the utilization of evergreen broad-leaved species with high ornamental value. A total of five treatments were installed (1m×22.5m), including 60g/㎡ and 80g/㎡ using two types mulching material with an overlapping and hole-drilling mulching method and these were compared to un-mulching treatment a total of planted 92㎡ attheWol-aTestSiteForestattheForestforBiomaterialsResearchCenterinJinju-si, Gyeongsangnam-dofor 10monthsusing3-years-oldQuercusglaucaThunb. In comparison with the control site, the 60g/㎡ overlapping method was about 1.9 times higher than the root collar diameter, but there was no statistical significance between the treatments. Healthy seedlings were found to meet these conditions due to high biomass values and below and T/R ratios of 3.0 or lower and H/D ratios of 7.0 or lower. Comparing the values of LWR, SWR, and RWR, which can be evaluated for seedling due to the mulching treatments, as compared to the control, the growth of the ground areas including leaves and stems was enhanced, but the growth of the underground areas containing roots tended to have high control values. Based on this, the SQI value, which can be evaluated for the comprehensive quality of seedlings, was found to be significantly different between the control site and the mulching treatment sites, confirming that the growth and growth improvement effects were achieved with mulching treatments. The chlorophyll content analysis showed that there was a significant difference from the control site, and it was judged that weed generation in the control acted as an environmental stress, causing a decrease in chlorophyll content. It was found that the overlapping 80g/㎡ of polypropylene mulching material generated about 4 times fewer weeds than the control, and the manpower required for the mulching test field and weeding were equal at 3.3 people/100㎡/1 day. Mulching treatments have demonstrated a significant difference in the promotion of growth and quality of the seedlings and are judged as an alternative that can reduce the economic burden incurred by the purchase of the supplies and the manpower required to weed forestry plantations.

Lateral Bud Suppression and Runner Plants Growth of 'Maehyang' Strawberry as Affected by Application Method and Concentration of IBA (IBA 처리방법과 농도에 따른 딸기 '매향'의 측아 발생 억제와 자묘 생육)

  • Hwang, Hee Sung;Jeong, Hyeon Woo;Lee, Hye Ri;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.29 no.1
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    • pp.80-88
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    • 2020
  • This study was conducted to examine the effect by application method and concentration of the indole-3-butyric acid (IBA), which is auxin-based plant growth regulator, on the growth and runner plants production of strawberry in the greenhouse. The seedlings of strawberry were transplanted in the pot (150 ×135 × 90 mm) filled with coir medium on April 12, 2019. The IBA was applied with a foliar spray or drench as 50, 100, 150, and 200 mg·L-1 (50 mL per plant), respectively. The treatment was started on April 29, 2019. The foliar spray and drench treatment of IBA were repeated at 2-week intervals for 9 weeks from the start date of treatment. At 9 weeks after treatment, the petiole length of mother plants was the shortest in the control. The number of runner plants showed a tendency to decreased in the foliar spray. The number of lateral buds showed a tendency to decreased in the IBA treatment, and the least in the foliar with 100 mg·L-1. There was not significantly difference in the fresh and dry weights of the first and second runner plants. However, in the third runner plants, the fresh and dry weights were the greatest in the drench with 100 mg·L-1. Therefore, when considering the growth of third runner plants and lateral bud suppression, the drench with the 100 mg·L-1 could be better application method and concentration of IBA treatment for growth of the third runner plants and runner plants production of strawberry, and the results can be used as a basic research of plant growth regulator application to save the labor force and enhance the seedling quality in strawberry seedling stage.

Comparisons of Growth, Heading and Grain Filling Characteristics between Wet-hill-seeding and Transplanting in Rice (벼 무논점파재배와 이앙재배의 생육, 출수 및 등숙특성 비교)

  • Shon, Ji-Young;Lee, Chung-Kuen;Kim, Jun-Hwan;Yoon, Young-Hwan;Yang, Won-Ha;Choi, Kyung-Jin;Choi, Min-Gyu;Park, Hong-Kyu;Ko, Jong-Cheol;Kim, Yeon-Gyu;Kim, Chung-Kon;Yang, Woon-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.2
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    • pp.151-159
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    • 2012
  • Direct seeding of rice is a time- and laborsaving method, compared to machine transplanting. However, the area planted to direct-seeded rice is decreasing because of instability of seedling establishment, lodging, occurrence of weedy rice, and deterioration of grain quality. A newly developed wet-hill-seeding of rice, a type of direct seeding method, has shown features of growth similar to machine transplanted rice. This study was carried out to compare tillering pattern, heading characteristics and grain filling traits between transplanting and wet-hill-seeding in rice. Tillering of wet-hill-seeded rice was more vigorous than that of transplanted rice showing maximum tiller number 30~100% higher than transplanted rice. Wet-hill seeded rice showed later heading and shorter heading duration than transplanted rice. To find out the relationship between heading characteristics and grain filling traits, every panicle was tagged for flowering date, and the panicles on each heading date were inspected for grain filling traits. Wet-hill seeded rice and transplanted rice exhibited no significant difference in culm length, panicle length, the percentage of grain filling and perfect brown rice on each heading date during total heading period. Therefore, we conclude that wet-hill-seeding method is not inferior to machine transplanting in terms of seedling establishment, growth, grain filling and head rice yield.

Effect of Rice Seed Disinfection of Loess-sulfur on the Suppression of Bakanae disease caused by Fusarium fujikuroi (벼 키다리병 방제에 관한 황토유황의 종자소독 효과)

  • So, Hyun-Kyu;Kim, Yong-Ki;Hong, Sung-Jun;Han, Eun-Jung;Park, Jong-Ho;Shim, Chang-Ki;Kim, Min-Jeong;Kim, Seok-Cheol
    • Korean Journal of Organic Agriculture
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    • v.25 no.2
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    • pp.345-355
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    • 2017
  • This study was conducted to evaluate rice seed disinfection efficacy of loess-sulfur for the suppression of Bakanae disease caused by Fusarium fujikuroi. Rice seeds were treated at different concentrations of loess-sulfur, soaking time and temperature, and combination of hot-water treatment. Rice cultivar, Shindongjin harvested from Bakanae disease-infested area in 2015, was used. Loess-sulfur was treated as follows; concentration of undiluted solution, 2%, 1% and 0.5%; soaking time of 24 and 48 hours; treatment temperature of $20^{\circ}C$ and $30^{\circ}C$; hot water treatment or not. Optimal conditions of rice seed disinfection were selected soaking time of 48 hours and the suspension of 0.5% and 1% loess-sulfur by investigating seed germination and isolation frequency of Fusarium spp. on Komada agar medium in vitro, and were established 3 disinfection conditions as hot water ($60^{\circ}C$, 10 min.) + 1% loess-sulfur ($20^{\circ}C$, 48 hours), 1% loess-sulfur only ($30^{\circ}C$, 48 hours) and 1% loess-sulfur only ($20^{\circ}C$, 48 hours) through additional test in greenhouse. Above 3 conditions were verified by rice seedling box and paddy field test in the way of investigating Bakanae diseased plants (%) and healthy plants (%). Consequently, most effective rice seed disinfection conditions on Bakanae disease were combination of hot water and 1% loess-sulfur and loess-sulfur only at $30^{\circ}C$. Furthermore, treatments with these conditions showed control value of 100% were maintained from seedling to the heading stage in the field. However, treatment of 1% loess-sulfur only at $20^{\circ}C$ showed low control value of 78.2% in paddy field. Hot water only treatment turned out to be an effective disinfection method when conducted thoroughly with $60^{\circ}C$, 10 min. However, it was thought additional soaking process with loess-sulfur after hot water treatment served more high control effect against Bakanae disease when rice seeds were disinfected on a large scale. This results expected rice seed disinfection with loess-sulfur were effectively and easily usable method if farmers had only one of either hot water-disinfector or seed-disinfector. In addition, loess-sulfur is well-known to farmers, simple to manufacture method and cheap.

Screening method of varietal resistance to planthoppers labeled with radioisotope $^{32}P$ (II) (방사성 동위원소 $^{32}P$를 이용한 멸구류에 대한 품종저항성 검정방법에 관한 연구 (II))

  • Lee Jeong Oon;Kim Yong Heon;Park Joong Soo;Lippold P.C.
    • Korean journal of applied entomology
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    • v.20 no.3 s.48
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    • pp.173-175
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    • 1981
  • New screening method of varietal resistance by isotope $^{32}P$ was examined in these experiments. Insects were fed for 24 hrs. in the vials, with rice seedlings absorbed the solution if $0.6\~7{\mu}\;Ci\;^{32}P$ for 24 to 48 hrs. Feeding amounts of 3 species of plant-hoppers at the different stages and duration of infestation were investigated for effective process of screening method of the varietal resistance using isotope $^{32}P$. Feeding amount of $^{32}P$ of brown plant-hopper was observed for the different varieties. The results may be summarized as follows: 1. Brown planthopper fed greatest and the feeding amount were increased in order of white-backed planthopper and small brown planthopper. Female hoppers fed more than male. 2. Feeding amount was increased in order of adult, 5th instar, adult, 4th instar, 3rd instar 2nd instar and 1st instar. The duration of $24\~48\;hours$ is considered sufficient for insect infestation. 3. New screening method by $^{32}P$ was compared with seedling bulk screening method in view of feeding amount and plant reaction. Feeding amount of $^{32}P$ by brown planthopper in Milyang 47, resistant variety to this insect, was very low, while in TN 1, susceptible variety, it was very high about one hundred tines of Milyang 47.

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Studies on Direct Sowing-Dry Paddy Rice Culture in the Middle Part of Korea (중부지방에 있어서의 수도건답직파재배 기술체계확립에 관한 시험연구)

  • Jai-Hyoun Lee
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.7 no.1
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    • pp.1-29
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    • 1969
  • Experiments on rice concerning it s varieties, fertilization, seedling dates and herbicides have been conducted to determine the most desirable method of direct sowing cultivation on dry paddy field land in the middle part of Korea. The results obtained at the Office of Rural Development of Choongnam Province are as follows:. 1. Sixteen different derivatives from the main varieties of low land rice were cultivated on a dry paddy field by the direct sowing method; at the same time, a few varieties were tried adopting the common transplanting cultivation method. The yield and yield factors from these two groups were examined to give the following results: a) Compared to the common transplanting cultivation, the direct sowing method showed remarkably increased number of panicles while the number of flowers per panicle was shown to be significantly decreased. The maturing ratio was detected to be lowered. The yield horn them differed according to the different varieties : good yield was obtained from Hokwang while Norin #25 proved poor when compared with the common transplanting cultivation method. b) Among sixteen varieties tested, Sunsou, Norin #25, Jaigou, Hokwang, Palkueng and Gosi showed comparatively high yields, their yield being more than 325 kilograms per 10 Are, but Nampoong, Paldal, Nongkwang, Norin #29, Eunbangju #101 and Shiro gane showed less yield, their yield being less than 271 kilograms per 10 Are, the relations between the yield and the yield factors can be summarized as follows; Number of varieties and their rice yield. 1) The varieties which were great in the, number of panicles and high in yield=Jaigoun, Hokwang Palkueng and Gosi. 2) The varieties which were low in the number of panicles and high in yield=Sounsou and Norin #25. 3) The varieties which were great in the number of panicles and poor in yield=Eunbangju #101 and Sirogane. 4) The varieties which were poor in the number of panicles and poor in yield: Nampung, Paldal and Norin #29. Number of flowers per panicle and yield. 1) The varieties which were great in the number of flowers per panicle and high in yield: Sounsou, Norin #25 and Gosi. 2) The varieties which were poor in the number of flowers per panicle and high in yield ; Jaigoun, Hokwang and Palkueng. 3) The varieties which were great in the number of flowers per panicle and poor in yield: Paldal and Nampung. 4) The varieties which were poor in the number of flowers per panicle and poor in yield: Norin #29. Eunbangju #101 and Sirogane. Maturing ratio and yield. 1) The varieties which were high in the maturing ratio and high in yield: Jaigoun, Sounsou, Norin #25 and Palkueng. 2) The varieties which were low in the maturing ratio and high in yield: Hokwang and Gosi. 3) The varieties which were early maturing rat io and low in yield: Hokwang and Gosi. 4) The varieties which were late maturing ratio and poor in yield: Eunbangju #101, Nampungand Sirogane 1, 000 grain weight and yield. 1) The varieties which were heavy in 1, 000 grains weight and high in yield=Norin #25 and Hokwang. 2) The varieties which were light in 1, 000 grains weight and high in yield=Sounsou and Jaigoun. 3) The varieties which were heavy in 1, 000 grains weight and poor in yield=Nongkwang and Eunbanju. 4) The varieties which were light in 1, 000 grains weight and poor in yield=Norin #29 and Sirogane. 2. The experiment on fertilization showed that the most desirable amount to be given per 10 Are was 10 kilograms of Nitrogen, 5 kilograms of phosphate and 6 kilograms of potassium; and when the Nitrogen given exceeded 8 kilograms, its effect was better when given in amsll consecutive (split) amounts, while the maturing ratio and the number of the flowers per panicle increased when Nitrogen was given in large amount during the later stage of growth of rice. 3. The experiment on the date and amount of seedling showed that the tested variety, Sunsou gave the best results when planted on the days between 25 April and 10 May. Eight liters per 10 Are were preferable if planted early and 12 liters per 10 Are if planted late. The reason why the later planting gave a lower yield was that the number of flowers per panicle was fewer. 4. The experiment on the irrigation for rice with direct sowing cultivation immersed in water showed that it was the most satisfactory when irrigated on 25th June, 55 days after its seedling, its plot giving the best yield. The plots 10th June and 15th July showed just as good results. However, irrigated later, than 15th July it showed lower yields. 5. Compared to the yield of the plot controlled by the common method, the yield from the plots treated with chemical herbicide such as LOROX, TOK, PCP, SWEP, Mo-338 on dry condition soil seemed poorer, but significant difference was not found statistically. On the other hand in the case where chemical herbicides such as TOK, Mo-338, Stam F-34 or ORDRAM were used after irrigation, the yield from the ORDRAM and TOK treated plots did not show significant differences compared to the common hand weed controling method, but those treated with chemicals other than the above showed a lower yield.

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Convenient Screening Method of Chinese Cabbage for Resistance to Plasmodiophora brassicae Using Soil-Drenching Inoculation (관주 접종법을 이용한 효율적인 배추 뿌리혹병 저항성 검정법)

  • Jo, Su-Jung;Jang, Kyoung-Soo;Choi, Yong-Ho;Kim, Jin-Cheol;Choi, Gyung-Ja
    • Research in Plant Disease
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    • v.16 no.3
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    • pp.279-284
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    • 2010
  • Clubroot caused by Plasmodiophora brassicae is a widespread disease that causes serious problems in many brassica growing areas. To establish more simple and reliable clubroot screening method of Chinese cabbage to P. brassicae using soil-drenching inoculation, the development of clubroot on Chinese cabbage according to several conditions such as soil type, inoculum concentration of P. brassicae GN-1 (race 9), plant growth stage and incubation period was studied. In a commercial horticulture nursery media soil (CNS), disease severity of the seedling according to inoculum concentration increased in a dose-dependent manner, but did not in mixture of CNS and upland soil (1:1, v/v). To facilitate and acquire precise result of resistance screening of Chinese cabbage to clubroot, 10-day-old seedlings should be inoculated by drenching the spore suspension of P. brassicae to give inoculum density of $4.0{\times}10^8$ spores/pot. To develop the disease, the inoculated seedlings were incubated in a growth chamber at $20^{\circ}C$ for 3 days, and then cultivated in a greenhouse ($25{\pm}5^{\circ}C$) for five weeks. Under the optimum conditions, 25 clubroot-resistant (CR) and 3 clubroot-susceptible (CS) cultivars were tested for resistance to P. brassicae. All CR cultivars showed very clear resistance response, on the other hand all CS cultivars severly infected with the pathogen. The results suggest that this method is efficient screening method of Chinese cabbage for resistance to clubroot disease.

An Evaluation Method for Sesame (Sesamum indicum L.) Resistance to Phytophthora nicotianae var. parasitica (참깨발병 품종저항성 검정방법 연구)

  • Seong Ho, Choi;Eui Kyoo, Cho;Young Am, Chae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.32 no.2
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    • pp.173-180
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    • 1987
  • Symptom development and disease severity of Phytophthora blight in the sesame plants varied depending upon age of the plants tested, inoculation method, watering method, and inoculum density in both susceptible Suweon 9 and Suweon 26 and moderately resistant B-67 and IS 103 sesame lines to Phytophthora nicotianae var. parasitica when inoculated. However, successful differentiation of the sesame lines for varietal resistance was possible using 20-day old seedling, inoculation by soil infestation, saturated soil water condition by half immersion of pots in water tank, and 200 sporangia per one ml of inoculum. Spraying or soil inoculation to 70-day old plants also was effective in differentiating the varietal resistance. By the screening method Suwon 26 showed 100% diseased plants and symptom severity index 9.0, while B-67 showed 20% diseased plants and symptom severity index 1.7. The rating scale given was from 0 through 9. For example, the scale 0 signified no symptom development, 5 signified discoloration of basal part of stem, and 9 signified discoloration of stem more than 10 cm high above the soil surface with blighted leaves. Differentiation in symptom severity also was made by percentage of the lesion area. Results evaluated using both parameters were well corresponded in varietal reaction of sesame to Phytophthora blight.

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