• Journal of Periodontal and Implant Science
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• v.37 no.1
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• pp.115-124
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• 2007

Silica is known as a promising osteoconductive material, and polycaprolactone is a bioactive and degradable material. The purpose of this study was to monitor the differentiation of MC3T3-E1 cells cultured on the layer-built silica/poly caprolactone non-woven fabric produced by electrospinning. Non-woven fabric (silica, polycaprolactone, PSP, SPS) was made by electrospinning and they were inserted in the 48 well cell culture plate. MC3T3-E1 cells were prepared by subculture. Cells were seeded to each well $1{\times}10^5$ concentration per well. Dulbecco's modified eagle medium with 10% FBS and 1% antibiotic-antimycotic solution was used. Confocal laser scanning microscope was taken 4 hours after incubation (95% air. 5% $CO_2$, $37^{\circ}C$). Cell proliferation was monitored by spectrophotometer on 1, 7, 14 days, and the morphology of the growing cells was observed by field emission scanning electron microscope. To monitor the differentiation of osteoblasts on the materials, MC3T3-E1 cells were incubated in 48 well culture plate after seeding with the density of $1{\times}10^5$ concentration. Then ELISA kit & EIA kit were used on to assess osteocalcin and osteopontin expression respectively. The other conditions were the same as above. MC3T3-E1 cells were proliferated well on all of the materials. There were no statistical differences among them. The osteopontin expression of silica, PSP, SPS was significantly higher than other groups on day 3 (p/0,05), but after that time, there were no statistically signigicant differences. The osteocalcin expression was significantly higher in silica and PSP than other groups on day 14. These findings show that PSP was as good as silica on the effect of osteoblast differentiation. The PSP non-woven fabric may have the possibility as bone graft materials.

• Journal of Gastric Cancer
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• v.1 no.3
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• pp.180-186
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• 2001

Purpose: The prognosis for early gastric cancer (EGC) is favorable, and the 10-year disease-specific survival rate is reported to be around $90\%$. The absolute number of recurred EGC is too small to assess the risk factors, so recruitment of a large number of cases for statistical analysis is very difficult. We carried out this study to analyze the incidence and the patterns of recurrence of EGC and to identify the clinicopathological risk factors for recurrence of EGC. Materials and Methods: The authors retrospectively investigated the follow-up records of 1418 patients who underwent a curative resection for EGC from Jan. 1984 to Dec. 1999 at the Korea Cancer Center Hospital and analyzed them with special reference to cancer recurrence. Results: In this retrospective study of 1418 cases, 43 patients died of a recurrence of gastric cancer, and 105 patients died of unrelated causes. The five-year and the ten-year overall survival rates were $89.6\%$ and $81.7\%$, respectively, while the five-year and the ten-year diseasespecific survival rates were $96.5\%$ and $94.3\%$, respectively. The recurrence patterns of the 45 recurred EGC were hematogenous metastasis (19 cases), lymph node (L/N) metastasis (8 cases), locoregional recurrence (2 cases), peritoneal seeding (3 cases), and combined form (13 cases). The mean time interval to recurrence was 38.6 months, and the number of delayed recurred cases after 5 years was 10 ($22.2\%$). Of the clinicopathologic factors, depth of invasion, L/N metastasis, macroscopic type, lymphatic invasion, and vessel invasion, were significant risk factors in the univariate analysis. However, in the multivariate analysis, only L/N metastasis was an independent prognostic factor. Conclusion: Based on the results of this study, L/N metastasis is an independent prognostic factor. Thus, in patients with node-positive disease, adjuvant therapy might be considered, and long-term close follow-up might facilitate early detection and treatment of recurrent disease due to delayed recurrence.

• Journal of the Korea Society of Computer and Information
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• v.20 no.4
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• pp.131-138
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• 2015

One of the most conspicuous characteristics of a business model that pursues expanding customer relationship is that it tries to lock in customers by encouraging them to repeat purchase in the long-term with the help of "Follow" function in Social Networking Service (SNS), which enables producers to automatically register the customers as potentially important ones and to offer them customized marketing services. In the value chain of the agriculture sector, producers of agricultural products can use SNS functions to provide loyal customers with valuable information and experiences such as the real-time information of their farm and products, hidden stories about the whole process from seeding to harvesting, and the storage and cooking methods of their products. These activities help the producers invoke customers' desire to live in the farm and to grow the products themselves. They also raise the accessibility of the producers' websites as customers are able to share a variety of news and knowledge such as the release of new products. This means that the producers's websites are now functioning to enable the producers to perform sales and promotion related activities. It is a big leap from the traditional e-commerce business model where sales and promotion of a product were separated and could be connected only through outside links. This two-way, viral characteristics of marketing services using SNS facilitate customers to share product information and their purchase experience with each other, which leads to more effective and efficient communication within the customer community.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.6
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• pp.532-539
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• 1991

This study was conducted to screen genetic stock for resistant to pod shedding. Materials used were 7 recommended varieties in Korea and 211 introduced varieties containing 94 Virginia type, 99 Spanish type and 18 Valencia type varieties. the days from seeding to first anthesis. number of mature pods per plant. 100 seeds weight and shedded pod ratio per total mature pod at harvesting were investigated. And the breaking tensile strength required to detach a pod from its gynophore was measured at a proper time for harvesting of each variety. In addition. factors related to pod shedding were discussed. The first anthesis of Spanish and Valencia types were earlier than that of Virginia type, but Korean recommended varieties which were recognized as Virginia type had relatively earlier anthesis. The varieties of Spanish type yielded more pods compared with those of Valencia and Virginia types. but Korean recom-mended varieties yielded slightly more pods than the others. Seeds weight of Korean recommended varieties was larger than not merely those of Spanish and Valencia types which were small seeds but also those of Virginia type which were large seeds. The breaking tensile strengths between pod and gynophore were various among the varieties belonging to same type. The strength was relatively strong in the Spanish type, but weak in both Virginia type and Korean recommended varieties. Therefore, pod shedding in the varieties of Spanish type was low and high in Virginia type and Korean recommended varieties. There were positive or negative correlations at the 0.1 % significan level among the days to first anthesis. number of pods per plant. seed weight, breaking tensile strength and shedding ratio. The pod shedding is considered a compound character that is affected by the factors such as breaking tensile strength between pod and gynophore, number of pods per plant and seed size.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.250-250
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• 2017

Studies were conducted to select the optimum sowing date and planting density of sesame in the middle area of Korea. To select the optimum sowing date, sesame seeds sowed from 20 April to 7 July with 15-day intervals. To select the optimum planting density, sesame seeds investigated under four different planting distances ($30{\times}10,\;30{\times}15,\;30{\times}20$, and $30{\times}25cm$)respectively in the experimental field. As seeding date was delayed, days to emergence were shortened flowering and maturing date were delayed. Delayed sowing date resulted in decreased length, capsule setting stem length and number of capsules, and branchs per plant. Number of Capsules was high sowing date on 5 May in the range of 90~95ea/plant in sesame. Also yield of sesame seeds was most high on 5 May in the range of 142kg/10a by sowing date. Sowing date up to 5 May showed no effect on grain yield, but from 5 June to 5 July decreased 27%, 68% and 86%, respectively. For all planting distances, weight of 1,000 grain was not significantly different. However, number of branches and capsules tended to increase. Number of Capsules was high planting distance of $30{\times}20cm$ and $30{\times}25cm$ in the range of 146.7~165.7ea/plant in the Geonbaekkae. Areumkkae also showed the same tendency on planting distance of $30{\times}20cm$ and $30{\times}25cm$ in the range of 122.0~147.5ea/plant, respectively. Yield of Geonbaekkae and Areumkkae seeds was most high 116kg/10a, 117kg/10a, respectively on planting distance $30{\times}20cm$. Decreased in the planting distance of sesame has increased the incidence of disease and lodging. Based on the results, we suggest a planting distance of $30{\times}20cm$ maximal growth and yield of sesame in the middle area of Korea. Considering growth characteristics, sesame yield ability, the optimum sowing date was 5 May and optimum planting pattern was founded to be two rows planting in one ridge and planting densities was $30{\times}20cm$.

• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.383-390
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• 1998

The purpose of this study was to evaluate the effect of new materials with forest topsoil for hydroseeding measures that is environmentally and ecologically stable revegetation measures on rock cut-slopes. Field hydroseeding experiment was used with a completely randomized design at highway rock cut-slopes in April, 1997. Results obtained in this study were summarized as follows: Particle size distribution of 3 mixed-soil materials that consisted of forest topsoil, decomposed granite soil, compost, sludge, and bottom ash did not show a significant difference. As appending the amount of forest topsoil, soil bulk density was increased. Soil hardness was slightly increased in early period, and then decreased with the flourishing of plants. The number of individuals increased, more than $3,000seedlings/m^2$, after 1 month, and it was decreased as time passes because of competition between the seeded species and the naturally emerged species. In addition to the seeded species, seeding plot has more than 6 species (Rubus crataegifolius, Eleusine indica, Erigeron canadensis, Lycopersicon esculentum var. cerasiforme, etc.) per $m^2$ were naturally appeared in the first year. From the viewpoint of species diversity promotion, the capability of using forest topsoil as seed bank sources was high. In order to apply in the field, the investigation and analysis of topsoil availability (quantity and quality of seed source, soil texture and organic composition) should be carried out before-hand.

• Journal of Korean Society of Forest Science
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• v.97 no.4
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• pp.398-407
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• 2008

This study is to give management state information about changes of income and net profit due to the fluctuations of chestnut prices to someone who will manage a chestnut orchard or has been managing a chestnut orchard in the market opening time. This study, hence, evaluated 133 households who manage a chestnut orchard at Jin-ju, San-cheoung in Gyeong-nam, Gu-rae in Jeon-nam, Gong-ju, Bu-yeo, and Cheoung-yang in Chung-nam. This study analyzed three scenarios by chestnut prices and by tree ages to provide informations for working expenses, production cost, income and net income to cultivators. In case of chestnut of one thousand won, the income of chestnut trees generated from nine ages and the net income is obtained from ten ages and turned into deficit from twenty-eight ages. In case of chestnut of one thousand and five hundred won, the income of chestnut trees generated from seven ages and the net income is obtained from nine ages. In case of chestnut of two thousand won, the income of chestnut trees generated from six ages and the net income is obtained seven nine ages. We calculated out labor costs and material cost and figured out management cost by tree ages. Namely two man and twenty woman are employed in harvesting 3,000 kg by hectare from ten till eighteen ages maximum harvesting seasons. Material cost is fertilizer cost, agricultural chemicals cost and seeding cost.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.1
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• pp.1-7
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• 2015

The photochemical characteristics were analyzed in the context of sowing time and different levels of fertilized nitrogen during the maize (Zea mays L.) growth. When maize was early sawn, the fluorescence parameters related with electron-transport, in photosystem II (PSII) and PSI, were effectively enhanced with the higher level of fertilized nitrogen. Highest values were observed in maize leaves grown in double N-fertilized plot. The photochemical parameters were declined in the progress of growth stage. In early growth stage, the fluorescence parameters were highest, and then reduced to about half of the parameters related with electron transport on PSII and PSI at middle and late growth stages. In 1/2 N plot, the photochemical energy dissipation was measured to 13% in term of active reaction center per absorbed photon resulting in decrease in performance index and driving force of electron. This decrease induced to lower the photochemical effectiveness. In 2 N plots, the electron transport flux from $Q_A$ to $Q_B$ per cross section and the number of active PSII RCs per cross section were considerably enhanced. It was clearly indicated that the connectivity between photosynthetic PSII and PSI, i.e. electron transport, was far effective.

• Archives of Plastic Surgery
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• v.36 no.4
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• pp.380-384
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• 2009

Purpose: Autologous vessels remain the gold standard for vascular grafts in microanastomoses. However, they are sometimes unavailable and have a limited long - term patency. Synthetic vessels have high success rates in large - diameter reconstructions but failed when used as small - diameter grafts due to graft occlusion. It has been proved that endothelial cell seeding improves prosthesis performance and long - term patency. Among polyurethane, PET and ePTFE, polyurethane has the best affinity to endothelial cells and mechanical properties closest to human vessels. We examined the ability of endothelial cells to attach to a polyurethane graft manufactured by the electrospinning method. Methods: Endothelial cells, which were cultured from porcine internal jugular veins, were attached to polyurethane grafts with an internal diameter of 3 mm. The same cells were attached to allogeneic decellularized porcine internal carotid artery grafts as controls. Both of the 10 mm - long grafts were exposed to endothelial cells in a well for 1 hour. Each well contained $2{\times}10^5$ endothelial cells. The graft materials were rotated through 90 degrees every 15 minutes in order to minimize the effect of gravity. The extent of cell attachment was examined with the MTT assay. Results: The MTT assay showed good incorporation of endothelial cells into both grafts. For the evaluation of affinity, the number of attached cells was counted at 10 fields of microscopic examination with ${\times}40$ magnification. Endothelial cells adhered more to polyurethane grafts (mean, $127.4{\pm}6.2cells$) compared to porcine artery grafts (mean $45.8{\pm}5.1cells$)(p<0.05,Mann - Whitney test). Conclusion: In this study, we attached porcine endothelial cells to polyurethane grafts, manufactured by electrospinning. The grafts exhibited a better affinity to endothelial cells than allogeneic decellularized porcine internal carotid artery grafts. It is suggested that the time required for endothelial cells to attach to decellulized artery grafts may be longer than that which is required for attachment to polyurethane grafts.

• Korean Journal of Weed Science
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• v.8 no.3
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• pp.244-249
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• 1988

Some experiments were conducted to utilize the raphanus test as a bioassay method for plant growth regulaters and hormonal herbicides. Among seven radish cultivars, the response of Jangbaegminong was the most sensitive and quantitative to ${\alpha}$-NAA and its coefficient of variation value was the lowest. The optimum application time of test compounds was estimated seven to nine days after seeding. After ${\alpha}$-NAA treatment the curvature angle of radish cotyledonary petiols was sharply increased to the maximum value at 24 hours after and was changed by light and temperature conditions. The curvature response was observed by application of ${\alpha}$-NAA, phenoxy and indole compounds but often plant growth regulators, $GA_3$ benzyladenine, ABA and homobrassinolide was not detected by this method. So this bioassay was useful to evaluate the activity of a compound as an auxin or a hormonal herbicide.