Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

A study on the differentiation of MC3T3-E1 incubated on the layer-built silica/polycaprolactone non-woven fabric produced by electrospinning

전기방사법으로 제조된 실리카/폴리카프로락톤 적층형 부직포에 배양한 골아 세포의 중식, 분화에 관한 연구

  • AN, Min-Kuk (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Kim, Kyoung-Hwa (Department of Periodontology, School of Dentistry, Seoul National University, BK21 Craniomaxillofacial Life Science) ;
  • Kim, Tae-II (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Lee, Yong-Moo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Rhee, Sang-Hoon (Department of Dental Biomaterials Science, School of Dentistry, Seoul National University) ;
  • Ku, Young (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Han, Soo-Boo (Department of Periodontology, School of Dentistry, Seoul National University) ;
  • Seol, Yang-Jo (Department of Periodontology, School of Dentistry, Seoul National University)
  • 안민국 (서울대학교 치의학대학원 치주과학교실) ;
  • 김경화 (서울대학교 치의학대학원 치주과학교실, 서울대학교 BK21 치의학생명과학사업단) ;
  • 김태일 (서울대학교 치의학대학원 치주과학교실) ;
  • 이용무 (서울대학교 치의학대학원 치주과학교실) ;
  • 이상훈 (서울대학교 치의학대학원 치과생체재료학교실) ;
  • 구영 (서울대학교 치의학대학원 치주과학교실) ;
  • 류인철 (서울대학교 치의학대학원 치주과학교실) ;
  • 정종평 (서울대학교 치의학대학원 치주과학교실) ;
  • 한수부 (서울대학교 치의학대학원 치주과학교실) ;
  • 설양조 (서울대학교 치의학대학원 치주과학교실)
  • Published : 2007.03.30
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Abstract

Silica is known as a promising osteoconductive material, and polycaprolactone is a bioactive and degradable material. The purpose of this study was to monitor the differentiation of MC3T3-E1 cells cultured on the layer-built silica/poly caprolactone non-woven fabric produced by electrospinning. Non-woven fabric (silica, polycaprolactone, PSP, SPS) was made by electrospinning and they were inserted in the 48 well cell culture plate. MC3T3-E1 cells were prepared by subculture. Cells were seeded to each well $1{\times}10^5$ concentration per well. Dulbecco's modified eagle medium with 10% FBS and 1% antibiotic-antimycotic solution was used. Confocal laser scanning microscope was taken 4 hours after incubation (95% air. 5% $CO_2$, $37^{\circ}C$). Cell proliferation was monitored by spectrophotometer on 1, 7, 14 days, and the morphology of the growing cells was observed by field emission scanning electron microscope. To monitor the differentiation of osteoblasts on the materials, MC3T3-E1 cells were incubated in 48 well culture plate after seeding with the density of $1{\times}10^5$ concentration. Then ELISA kit & EIA kit were used on to assess osteocalcin and osteopontin expression respectively. The other conditions were the same as above. MC3T3-E1 cells were proliferated well on all of the materials. There were no statistical differences among them. The osteopontin expression of silica, PSP, SPS was significantly higher than other groups on day 3 (p/0,05), but after that time, there were no statistically signigicant differences. The osteocalcin expression was significantly higher in silica and PSP than other groups on day 14. These findings show that PSP was as good as silica on the effect of osteoblast differentiation. The PSP non-woven fabric may have the possibility as bone graft materials.

Keywords

References

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