• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.1
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• pp.81-87
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• 2019

The investigation of mycelial growth for artificial cultivation in T. albuminosus determined that within PDA, YM, MEA medium, both KNCAF 9001 and 9002 strains showed the highest mycelial growth in MEA media with 89, 81 mm, respectively, followed by YM, PDA in respected order. Furthermore, optimal mycelial growth of 89 and 77 mm in 9001 and 9002, respectively, at 25 ℃. Mycelial growth rapidly decreased at 30 ℃, but it was confirmed that it survived at 40 ℃. At pH 6, mycelial growth was fast with growth of 89 and 88 mm for both 9001 and 9002, respectively. In the case of 9001, the addition of with cotton seed hull(80%) and rice bran (20%) each other resulted in maximum productivity of 99 mm/10 days. The result differed in order of corn cob(97 mm), oak sawdust(96 mm) and poplar sawdust(93 mm). Ultimately, the result confirmed that addition of rice bran in comparison to bran resulted in higher yield. Even with identical medium component, 9002 showed the same tendency as 9001 but its growth was relatively slower than 9001. The effect of mycelial growth on the moisture content of the medium was best at 101. 94 mm/10 days when the moisture content of 9001 and 9002 was 65%. Mycelial growth rate tended to be slower when the moisture content was higher or lower than 65%.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.269-269
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• 2022

Pre-harvest sprouting (PHS) on rice panicles is getting problematic in recent several years in Korea due to climate changes such as high temperature and more frequent typhoons during harvesting season. PHS negatively affects grain quality severely and also yield. Genetic improvement of Korean varieties (Oryza sativa ssp. japonica) through a marker assisted-backcross breeding (MAB) with the known PHS resistant genes must be one of ideal solutions. However, the final breeding products of MAB occasionally exhibit unwanted traits, especially the cross between genetically distant parents. This might be caused by linkage drag and/or presence of the gene-unlinked donor introgressions, resulting that the final products could not be released to the farmers. The major PHS resistance gene, Sdr4 (Seed dormancy 4) originated from an indica cultivar, Kasalath was selected as a donor gene. In order to avoid unexpected phenotypes in the breeding products, we performed a precision marker-based breeding (PMBB) consisting of foreground, recombinant, and background selections (FS, RS, and BS) which aim to develop 'single small introgression lines' (~100 kb introgression). Korean varieties (Ilpum and Gopum) were crossed with Kasalath. We developed Sdr4-allele specific markers for FS and a set of polymorphic flanking markers near the Sdr4 (-350kb and +420kb) for RS. To minimize linkage drag, the small introgression (< 125kb) containing Sdr4 was selected in Ilpum background (BC₂F₄) through 1^st RS with ~1,200 F₂ or BC₁F₂ plants (one side trimmed) and then 2^nd RS with ~1,000 progenies from the 1^st RS selected plants (another side trimmed). After RS, the selected lines were genotyped by using Infinium 7K SNP chip to detect other donor introgressions and the lines were backcrossed. Currently BS is on-going from the backcross-derived progenies with BS markers to remove residual introgressions. During the PMBB process, genetic effect of Sdr-4-Kasalath allele was confirmed in Ilpum and Gopum backgrounds by PHS phenotyping using the segregating BC₂F₃ or BC₁F₄ materials. The Sdr4 PMBB lines in Ilpum background (< 125kb introgression) will be valuable genetic resources to improve PHS resistance in modem popular temperate japonica varieties.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.4
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• pp.295-300
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• 2008

This experiment was conducted to compare forage production and feed value of winter forage crops at the experimental field of Livestock Division, National Institute of Animal Science from 2003 to 2004 in Jeju. The experiment was arranged in slit plot design with three replications. Main plots consisted of two cutting times, one time cutting and two times cutting. Sub plots consisted of 5 different seed combinations, T1 (Italian ryegrass), T2 (Oat), T3 (Italian ryegrass 25+Oat 75%), T4 (Italian ryegrass 50+Oat 50%), T5 (Italian ryegrass 75+Oat 25%). One time cutting showed the highest dry matter (DM) yield by 18,680 kg/ha. Tow times cutting showed the highest crude protein (CP) content by 11.97%. The all treatments have narrow range of total digestible nutrient (TDN) from 60.6% to 70.4%.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.3
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• pp.156-164
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• 2004

This study was conducted to evaluate the effect of the application of green manure in the form of either green barley and Chinese milkvetch in reducing the amounts of N fertilizers and conventional fertilizers needed for paddy rice. Prior to rice transplanting, the green barley and Chinese milkvetch as a green manure produced respectively $668kg\;10a^{-1}$ and 3,492kg\;$10a^{-1}$ in fresh shoot weight basis. Calculated nitrogen content from harvested green manures was 3.9 and $17.8kg\;10a^{-1}$, respectively. Plant height and tiller number of rice increased when two kinds of green manure incorporated into soil. Above mentioned parameters also increased with increasing amounts of N fertilizers at both ear formation and heading stage of rice. Rice grain number was not affected by green manures treatment but increased when N fertilizers were applied. Although rice panicle and grain number increased with green manure treatments and fertilizer applications, whereas the percentage of ripened grain decreased. Chinese milkvetch with additional N fertilizer applications increased brown rice yield from 1 to 5% compared to rice yields in plots where non-green manure with the conventional amount of fertilizer application was applied. Rice treated with Chinese milkvetch and 30% of the conventional N fertilizer rate yielded the same as rice fertilized conventionally. During the rice growing season, $NH_4-N$ content of paddy soil was higher in green manures treatment than non-green manure one. Average $NH_4-N$ content in paddy soil drastically decreased after heading stage below $5.7mg\;kg^{-1}$ in non-green manure treated plots. While on the other, $NH_4-N$ content in soil slowly decreased in plots those were treated with green manures at harvesting stage, average $NH_4-N$ content was still greater than $5.5mg\;kg^{-1}$. Nitrogen content of rice shoot and brown rice seed was higher in green manure treatment.

• Journal of Food Hygiene and Safety
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• v.22 no.4
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• pp.359-364
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• 2007

This study was conducted to determine the optimum condition of extraction and antioxidant activity of ethanol extracts of Epimedium koreanum Nakai based on the icariin quantity. Also, further organic solvent fractions of hexane, chloroform, ethyl acetate and butanol were obtained from the ethanol extract of Epimedium koreanum Nakai at different temperatures. Total ethanol extraction yield of wild grape seed ranged from 11.8% to 39.3% depending on the concentration of icariin as well as different ethanol concentration, extraction temperature and time condition. Among different extraction temperatures and time, the highest extraction yield of 39.3% was obtained at 70% ethanol for 3 hour at $50^{\circ}C$ in the sample containing the 0.596% icariin (KE9412). In the meantime, the strongest free radical scavenging effect $(RC_{50})$ with $18.9{\mu}g/mL$ in the KE9412 sample was observed in 70% ethanol extract of Epimedium koreanum Nakai extracted for 7 hour at $70^{\circ}C$, while $RC_{50}$ with $35.2{\mu}g/mL$ was observed in the KE9405 (0.20% icariin content) sample at same condition. Also, antioxidant activity of ethanol extracts of Epimedium koreanum Nakai increased as icariin concentration increased. Among each fraction obtained from organic solvents, butanol fraction was found to have the strongest $RC_{50}\;(39.2{\mu}g/mL)$ and followed by ethylacetate $(49.0{\mu}g/mL)$, water $(118.8{\mu}g/mL)$, ethylacetate $(119.8{\mu}g/mL)$, and chloroform fraction $(138.5{\mu}g/mL)$ respectively.

• Journal of the Korean Applied Science and Technology
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• v.35 no.1
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• pp.99-110
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• 2018

In this study, oil of Platycodon grandiflorum seeds was prepared using supercritical carbon dioxide extraction (SCE) and its physicochemical indices as a new edible oil were investigated. Compared to Soxhlet solvent extraction, SCE under the condition of 6,000 psi at $40^{\circ}C$ produced more oil, especially from the roasted seeds to 32.7%. TLC analysis showed triacylglycerols accounted for most of the oil obtained from roasted Platycodon grandiflorum seeds by SCE similarly to commercial soybean oil or perilla seeds oil. The oil had highly unsaturated lipid with considerable amount of linoleic acid(73.27%) much more than two commercial oils followed by oleic acid(13.16%). Physicochemical properties of the oil were as follows; specific gravity, 0.92; dynamic viscosity, 45.37 cP; refractive index, 1.48; color, L=47.30, a=-3.69, b=25.72; iodine value, 141.57 g $I_2/100g$ oil; saponification value, 191.21 mg of KOH/g of oil; acid value, 2.60 mg of KOH/g of oil. Among those, refractive index, viscosity and iodine value, which were related to unsaturation degree of lipid, were ranged between those of two commercial oils. The oxidation stability of oil(2.03 hr) was also ranged between less stable perilla seeds oil(1.79 hr) and more stable soybean oil(2.94 hr) based on the induction time measured by Rancimat assay. In addition to extraction yield increase, seeds roasting provided further benefits such as reductions of cholesterol ester content and acid value without change in fatty acid composition. In conclusion, oil was extracted from the roasted Platycodon grandiflorum seeds at high yield by supercritical carbon dioxide and it seemed to have proper characteristics as a edible oil.

• Horticultural Science & Technology
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• v.18 no.3
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• pp.334-341
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• 2000

To develop a nutrient solution for a closed hydroponic system in potato (Solanum tuberosum L.) 'Atlantic' and 'Superior' potatoes were grown with the nutrient solutions whose strengths were 0.25, 0.5, 1.0, and 1.5 of the concentration of the nutrient solution developed by the National Horticultural Experiment Station in Japan. The best results in potato growth and yield were obtained with 0.5 and 1.0 strength nutrient solutions, and nutrient compositions for potato were determined based on the 1.0 strength nutrient solution; $14.4me{\cdot}L^{-1}\;N,\;4.2me{\cdot}L^{-1}\;P,\;7.5me{\cdot}L^{-1}\;K,\;5.5me{\cdot}L^{-1}\;Ca$, and $3.5me{\cdot}L^{-1}\;Mg$ for stolon growth stage and $14.8me{\cdot}L^{-1}\;N,\;4.0me{\cdot}L^{-1}\;P,\;8.5me{\cdot}L^{-1}\;K,\;6.5me{\cdot}L^{-1}\;Ca$, and $3.0me{\cdot}L^{-1}\;Mg$ for tuber growth stage. To examine the suitability of the nutrient solutions developed for potato, the strengths of 1.0 (PS 1.0S), 0.75 (PS 0.75S), or 0.5 (PS 0.5S) were compared with half-strength of Japanese Horticultural Experiment Station' solution (JH 0.5S). Changes in pH, EC, and mineral concentrations in nutrient solutions depended more on solution strength and growth stage than on the type of nutrient solution. However, most elements in solution remained constant with plant age in PS 0.75S solution during stolon growth stage, and in PS 0.5S solution during tuber growth stage. The greatest growth and tuber yield was obtained in the standard strength solution (PS 1.0S), and potato solution developed in this experiment was recommended for hydroponic culture of potato in a closed system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.149-154
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• 1992

Buckwheat has been a popular favorite food crop in Korea for a long time. The objective of the study was to investigate the effects of climatic conditions and cultural methods on grain yields of summer buckwheat variety Sinnong 1 planted during the spring season in Suwon, Korea from 1989 to 1991. Frost minimum temperature of late April was -0.3$^{\circ}C$ in 1990 being very low as compared with 3.7$^{\circ}C$ of the normal year, and affected early growth of the seedlings emerging from the soil surface. In late May of 1990, the frost minimum temperature was 7.3$^{\circ}C$ being low as compared with 8.8$^{\circ}C$ of the normal year, and also induced cold injury to fertilization and grain filling. Total precipitation 374.5mm of mid and late June, 1990 provided serious damage to the grain filling and maturing buckwheat seeds and along with causing seed sprouting before harvest. However, the climates of 1989 and 1991 were very good for the growth and development of spring-sown buckwheats. When summer buckwheat cultivar Sinnong 1 was planted on April 20, 1989, its highest grain yields 268-292kg /10a were harvested from the plots of seeding rate 8kg /10a, drill seeding and polyethylene film mulching, and the mean grain yield of the plots was 238kg /10a in 1989, but 64.3kg in Suwon, and 40.2kg /10a in Muan in 1990. In 1991 maximum grain yield 277kg /10a was produced from the April 15 planted and vinyl-mulched plot, and 255kg /10a from the April 25 planted and non-mulched plot. Herbicide Alachlor-sprayed plots produced lower grain yields than no weed control and manual weeding plots. Mechanized drill-seeding saved 83～84% in planting hours as compared with manual broadcasting 21.6 hours /ha, and produced 9% more in grain yields from the two-season croppings of mechanized drill-seeding culture being 364kg /10a in total yields per year.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.2
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• pp.156-160
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• 2008

This study was conducted to find on the screening of a optimal variety after compare with characteristics of a colored soybean recommended and a vegetable soybean by pure line isolation from the indigenous variety in Chungnam area. The budding period in results compared with a budding property of testing varieties between the recommended colored soybean 6 varieties and the indigenous 3 varieties, was showed that it was delayed by increasing of a grain weight but the budding rate was not significant. And the harvest period was the most early day at the black colored soybean plot at the 30th July and was the latest day at the native variety (Nonsan jaerae) at the 21 th October. The stem length was showed a wide range from 25 to 104 cm, and the Asan jaerae in this result was the highest on 104 cm, but the Geomjeongolkong was the lowest on 25 cm. The number of pod per plant was searched about 9 to 111 range, and the most numerous plot in this experimental varieties was the Ilpumgeomjeongkong as 111 number, on the other hand, the least variety was the Asan jaerae as 9 number. The rate of more than 2 seed per pod in the vegetable soybean property was the highest at the Geomjeongolkong about 82%. Additionally, the Hwangkuemkong and Geomjeongkong 1 was relatively the high levels as 78% and 73%, respectively. At the pod yield per $1000\;m^2$ (10a) was the most much as 1,567 kg at the Ilpumgeomjeongkong. Also, the Sunheukkong and the Galmikong was 1,468 kg, 1,397 kg, respectively. Therefore, the Ilpumgeomjeongkong, Sunheukkong, and Galmikong were proper varieties for the vegetable cultivation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.3
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• pp.384-390
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• 2009

Extraction characteristics of citron (Citrus junos Sieb. ex Tanaka) seeds and functional properties of corresponding extract were monitored by response surface methodology (RSM). Maximum extraction yield of 20.23% was obtained at extraction temperature of $50.23^{\circ}C$, extraction time of 3.03 hr, and shaking velocity of 400.06 rpm. At extraction temperature, extraction time, and shaking velocity of $49.88^{\circ}C$, 2.72 hr, and 400.39 rpm, respectively, maximum polyphenol content was 4.37 mg/g. At extraction temperature, extraction time, and shaking velocity of $50.28^{\circ}C$, 3.42 hr, and 399.96 rpm, respectively, maximum electron donating ability (EDA) was 49.69%. Maximum nitrite scavenging activity (NSA) was 47.79% at extraction temperature, extraction time, and shaking velocity of $49.19^{\circ}C$, 0.68 hr, and 602.95 rpm, respectively. Based on superimposition of 3-dimensional RSM with respect to extraction yield, polyphenol, EDA, and NSA, optimum ranges of extraction conditions were extraction temperature of $50^{\circ}C$, extraction time of about 3 hr, and shaking velocity of 400 rpm.