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http://dx.doi.org/10.3746/jkfn.2009.38.3.384

Antioxidant Activity of Ethanol Extraction on Citron Seed by Response Surface Methodology  

Woo, Koan-Sik (National Institute of Crop Science, Rural Development Administration)
Jeong, Ji-Young (Dept. of Food Science and Technology, Chungbuk National University)
Hwang, In-Guk (Dept. of Food Science and Technology, Chungbuk National University)
Lee, Yoon-Jeong (Dept. of Food Science and Technology, Chungbuk National University)
Lee, Youn-Ri (Dept. of Food Science and Technology, Chungbuk National University)
Park, Hee-Jeong (Rural Development Administration)
Park, Eui-Seok (Metome Food Co. Ltd.)
Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.3, 2009 , pp. 384-390 More about this Journal
Abstract
Extraction characteristics of citron (Citrus junos Sieb. ex Tanaka) seeds and functional properties of corresponding extract were monitored by response surface methodology (RSM). Maximum extraction yield of 20.23% was obtained at extraction temperature of $50.23^{\circ}C$, extraction time of 3.03 hr, and shaking velocity of 400.06 rpm. At extraction temperature, extraction time, and shaking velocity of $49.88^{\circ}C$, 2.72 hr, and 400.39 rpm, respectively, maximum polyphenol content was 4.37 mg/g. At extraction temperature, extraction time, and shaking velocity of $50.28^{\circ}C$, 3.42 hr, and 399.96 rpm, respectively, maximum electron donating ability (EDA) was 49.69%. Maximum nitrite scavenging activity (NSA) was 47.79% at extraction temperature, extraction time, and shaking velocity of $49.19^{\circ}C$, 0.68 hr, and 602.95 rpm, respectively. Based on superimposition of 3-dimensional RSM with respect to extraction yield, polyphenol, EDA, and NSA, optimum ranges of extraction conditions were extraction temperature of $50^{\circ}C$, extraction time of about 3 hr, and shaking velocity of 400 rpm.
Keywords
citron (Citrus junos Sieb. ex Tanaka) seeds; polyphenol; DPPH radical scavenging activity; response surface methodology (RSM);
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