• Applied Microscopy
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• 제47권3호
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• pp.171-175
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• 2017

Negative staining has been traditionally used for exosome imaging; however, the technique is limited to surface topology only and can cause staining artifacts. Therefore, to analyze the internal structure of exosomes, we employed a method of block preparation, thin sectioning, and electron tomography. In addition, an automatic serial sectioning technique with 15-nm thickness through focused ion beam was employed to observe the three-dimensional structure of exosomes of various sizes. Cryo-transmission electron microscopy revealed the near-to-native structure of exosomes.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2004년도 추계 학술발표회 제16권2호
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• pp.667-670
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• 2004

This study describes a practical method of measurement of the sustained stress in concrete structures. In most cases, the sustained stress was determined by various theoretical calculations. However, the theoretical calculations can not always provide sufficient informations about current stress state having lots of uncertainty. Therefore, the present study proposes a practical measuring method, Partial Sectioning Method.

• Applied Microscopy
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• 제42권1호
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• pp.49-52
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• 2012

For TEM study of biological samples or polymers that are contained in organic structure, it is often required that the sample is prepared by using ultramicrotome and stained with proper agents to increase the contrast of organic structure. In this study, we investigated an efficient TEM sample preparation method for ductile heterogeneity material by using ultramicrotomy. Cryo-ultramicrotomy is a suitable method that is capable of rendering sample hardness for various ductile materials. However, it has several factors to consider, such as experimental cost, working time and finding the optimal staining conditions. To satisfy these considerations, we prepared TEM sample by using ultramicrotome without cryofunction, and secured the sample hardness by applying the staining process prior to ultrathin sectioning. The cross-linked polyethylene structure in the sample was stained with the 2% $RuO_4$ solution in a sealed test tube for 24 hours at $4^{\circ}C$. After the sample staining, ultrathin sections of sample were prepared using ultramicrotome. As a result, it was revealed that the difficulties associated with staining of ultrathin sections prepared by low-temperature conditions were improved. In addition, appropriate staining depth of sample could be selected for sectioning process. The quality of TEM sample obtained by using this method was better than that of cryo-ultramicroscopy. Finally, it is expected that our method could be effectively applied in TEM sample preparation for a variety of nano-bio convergence materials.

• 대한기계학회논문집B
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• 제24권10호
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• pp.1335-1342
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• 2000

Three-dimensional flow analysis and numerical optimization methods are presented for the design of an axial-flow fan. Steady, incompressible, three-dimensional Reynolds-averaged Navier-Stokes equations are used as governing equations, and standard k- ${\varepsilon}$ turbulence model is chosen as a turbulence model. Governing equations are discretized using finite volume method. Steepest descent method, conjugate gradient method and BFGS method are compared to determine the searching directions. Golden section method and quadratic fit-sectioning method are tested for one dimensional search. Objective function is defined as a ratio of generation rate of the turbulent kinetic energy to pressure head. Two variables concerning sweep angle distribution are selected as the design variables. Performance of the final fan designed by the optimization was tested experimentally.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 1999년도 유체기계 연구개발 발표회 논문집
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• pp.221-226
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• 1999

Three-dimensional flow analysis and numerical optimization methods are presented for the design of an axial-flow fan. Steady, Incompressible, three-dimensional Reynolds-averaged Wavier-Stokes equations are used as governing equations, and standard k-$\epsilon$ turbulence model is chosen as a turbulence model. Governing equations are discretized using finite volume method. Steepest descent method, conjugate gradient method and BFGS method are compared to determine the searching directions. Golden section method and quadratic fit-sectioning method are tested for one dimensional search. Objective function is defined as a ratio of generation rate of the turbulent kinetic energy to pressure head. Sweep angle distributions are used as design variables.

• 한국추진공학회지
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• 제1권1호
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• pp.104-110
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• 1997

고강도 알루미늄 합금 링롤재의 급냉, 링 팽창(expansion) 및 링 압축(compression) 응력제거처리 후 잔류응력을 예측하기 위하여 2차원 축대칭 열해석 및 탄소성 해석을 수행하였다. 급냉 및 응력제거처리 후 2단 과시효 처리(T73)된 링롤재에 대하여 3단계 절단법(Three step sectioning method)을 적용하여 링롤재의 두께에 따른 잔류응력 분포를 측정하였으며, 측정결과를 급냉 및 응력제거처리후 잔류응력 해석결과와 비교분석하였다. 링의 급냉후 원주 및 축방향의 잔류응력 해석값은 T73후 측정값과 비슷한 경향을 보였으며, 링의 내면과 외면에서 압축응력을 나타내었고 중심에서 인장응력을 나타내었다. 잔류응력은 링 팽창(T7351) 및 링 압축(T7352) 적용후 T73에 비해 현저히 감소하였으며, 축방향의 제거 효과가 원주방향보다 우수하게 나타났다. 또한 링 압축에 의한 제거효과가 링 팽창보다 크게 나타났다. 링롤재의 응력제거처리는 제거 효과 및 실용성 측면에서 링 압축 공정이 유리하며, 치수제어 및 장비용량 측면에서 링 팽창 공정이 유리하다는 결론을 얻었다.

• Steel and Composite Structures
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• 제33권4호
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• pp.489-507
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• 2019

In order to obtain high bearing capacity and good ductility simultaneously, a structural column with hybrid normal and high strength steel (HNHSS) welded box section has been developed. Residual stress is an important factor that can influence the behaviour of a structural member in steel structures. Accordingly, the magnitudes and distributions of residual stresses in HNHSS welded box sections were investigated experimentally using the sectioning method. In this study, the following four box sections were tested: one normal strength steel (NSS) section, one high strength steel (HSS) section, and two HNHSS sections. Based on the experimental data from previous studies and the test results of this study, the effects of the width-to-thickness ratio of plate, yield strength of plate, and the plate thickness of the residual stresses of welded box sections were investigated in detail. A unified residual stress model for NSS, HSS and HNHSS welded box sections was proposed, and the corresponding simplified prediction equations for the maximum tensile residual stress ratio (${\sigma}_{rt}/f_y$) and average compressive residual stress ratio (${\sigma}_{rc}/f_y$) in the model were quantitatively established. The predicted magnitudes and distributions of residual stresses for four tested sections in this study by using the proposed residual stress model were compared with the experimental results, and the feasibility of this proposed model was shown to be in good agreement.

• 한국추진공학회지
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• 제2권1호
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• pp.31-40
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• 1998

고강도 알루미늄 대형 링롤재의 급냉 및 요소제거 후 발생하는 잔류응력을 2차원 및 3차원 유한요소 해석하였고, 3단계 절단법에 의해 측정된 변형율과 ABAQUS의 요소제거 기능을 이용한 해석값을 직접 비교하였다. 급냉후 잔류응력 해석결과는 2단 과시효후 측정값과 비슷한 경향을 보였으나 그 절대값의 차이가 크게 나타났다. 이는 링 시편의 높이가 작아 측정값이 부정확하였기 때문이며, 링의 높이를 증가시켜 높이방향으로 균일한 응력분포를 갖도록 하면 그 차이가 감소될 것으로 판단된다. 잔류응력을 측정하는 3단계 절단 과정을 모사한 3차원 해석결과와 측정된 변형율을 직접 비교함으로써 실험과 해석의 정확성을 향상시킬 수 있도록 하였다. 이로부터 잔류응력이 내재하고 형상이 복잡한 대형 알루미늄 구조물의 기계가 공에 의한 변형을 정확히 예측할 수 있는 기반을 마련하였다.

• Journal of Welding and Joining
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• 제21권1호
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• pp.66-71
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• 2003

The release of residual stress by mechanical loading and unloading is often performed in the fabrication of box structure fur steel bridge. The proper degree of loading and unloading is significant at release method of residual stress by mechanical loading because that degree is changed by material and geometric shape of welded structure. Therefore, the simulation model that could exactly analyze the release of residual stress by mechanical loading is to be necessary. In this study, the non-linear behavior of weldments under external loading and unloading, such as the decrease and increase of structure stiffness, was investigated by monitoring of nominal stress and strain. Tensile loading and unloading test and the proper degree of stress relaxation was measured by sectioning technique using strain gauge. Analysis model that is indispensable for the effective application of MSR method was established on the basis of test and measurement result.

• 한국정밀공학회지
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• 제19권9호
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• pp.151-156
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• 2002

A new position correction method using four fiducials as reference points was developed and examined. It was aimed to calibrate nonlinear deformation by numerous error sources. A correlation for correction was derived from the geometric relationship between four fiducials and chip position. Compared with three points method, it exhibited more accurate correction, especially for inner area of a quadrilateral composed of four fiducial points. Its accuracy was found to be increased as fiducials moves outwardly within a printed circuit board (PCB) and/or as they form more rectangle-like shape As for arbitrarily nonlinear deformation, this method can be applied using more than five fiducials. In this case, local-area calibration is carried out by sectioning a board area into several rectangular are as.