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http://dx.doi.org/10.9729/AM.2017.47.3.171

Structural Analysis of Exosomes Using Different Types of Electron Microscopy  

Choi, Hyosun (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University)
Mun, Ji Young (BK21 Plus Program, Department of Senior Healthcare, Graduate School, Eulji University)
Publication Information
Applied Microscopy / v.47, no.3, 2017 , pp. 171-175 More about this Journal
Abstract
Negative staining has been traditionally used for exosome imaging; however, the technique is limited to surface topology only and can cause staining artifacts. Therefore, to analyze the internal structure of exosomes, we employed a method of block preparation, thin sectioning, and electron tomography. In addition, an automatic serial sectioning technique with 15-nm thickness through focused ion beam was employed to observe the three-dimensional structure of exosomes of various sizes. Cryo-transmission electron microscopy revealed the near-to-native structure of exosomes.
Keywords
Exosome; Electron tomography; Serial block face section through focused ion beam; Cryo-transmission electron microscopy;
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