• Applied Microscopy
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• v.47 no.3
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• pp.171-175
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• 2017

Negative staining has been traditionally used for exosome imaging; however, the technique is limited to surface topology only and can cause staining artifacts. Therefore, to analyze the internal structure of exosomes, we employed a method of block preparation, thin sectioning, and electron tomography. In addition, an automatic serial sectioning technique with 15-nm thickness through focused ion beam was employed to observe the three-dimensional structure of exosomes of various sizes. Cryo-transmission electron microscopy revealed the near-to-native structure of exosomes.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.11a
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• pp.667-670
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• 2004

This study describes a practical method of measurement of the sustained stress in concrete structures. In most cases, the sustained stress was determined by various theoretical calculations. However, the theoretical calculations can not always provide sufficient informations about current stress state having lots of uncertainty. Therefore, the present study proposes a practical measuring method, Partial Sectioning Method.

• Applied Microscopy
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• v.42 no.1
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• pp.49-52
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• 2012

For TEM study of biological samples or polymers that are contained in organic structure, it is often required that the sample is prepared by using ultramicrotome and stained with proper agents to increase the contrast of organic structure. In this study, we investigated an efficient TEM sample preparation method for ductile heterogeneity material by using ultramicrotomy. Cryo-ultramicrotomy is a suitable method that is capable of rendering sample hardness for various ductile materials. However, it has several factors to consider, such as experimental cost, working time and finding the optimal staining conditions. To satisfy these considerations, we prepared TEM sample by using ultramicrotome without cryofunction, and secured the sample hardness by applying the staining process prior to ultrathin sectioning. The cross-linked polyethylene structure in the sample was stained with the 2% $RuO_4$ solution in a sealed test tube for 24 hours at $4^{\circ}C$. After the sample staining, ultrathin sections of sample were prepared using ultramicrotome. As a result, it was revealed that the difficulties associated with staining of ultrathin sections prepared by low-temperature conditions were improved. In addition, appropriate staining depth of sample could be selected for sectioning process. The quality of TEM sample obtained by using this method was better than that of cryo-ultramicroscopy. Finally, it is expected that our method could be effectively applied in TEM sample preparation for a variety of nano-bio convergence materials.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.24 no.10
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• pp.1335-1342
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• 2000

Three-dimensional flow analysis and numerical optimization methods are presented for the design of an axial-flow fan. Steady, incompressible, three-dimensional Reynolds-averaged Navier-Stokes equations are used as governing equations, and standard k- ${\varepsilon}$ turbulence model is chosen as a turbulence model. Governing equations are discretized using finite volume method. Steepest descent method, conjugate gradient method and BFGS method are compared to determine the searching directions. Golden section method and quadratic fit-sectioning method are tested for one dimensional search. Objective function is defined as a ratio of generation rate of the turbulent kinetic energy to pressure head. Two variables concerning sweep angle distribution are selected as the design variables. Performance of the final fan designed by the optimization was tested experimentally.

• 유체기계공업학회:학술대회논문집
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• 1999.12a
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• pp.221-226
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• 1999

Three-dimensional flow analysis and numerical optimization methods are presented for the design of an axial-flow fan. Steady, Incompressible, three-dimensional Reynolds-averaged Wavier-Stokes equations are used as governing equations, and standard k-$\epsilon$ turbulence model is chosen as a turbulence model. Governing equations are discretized using finite volume method. Steepest descent method, conjugate gradient method and BFGS method are compared to determine the searching directions. Golden section method and quadratic fit-sectioning method are tested for one dimensional search. Objective function is defined as a ratio of generation rate of the turbulent kinetic energy to pressure head. Sweep angle distributions are used as design variables.

• Journal of the Korean Society of Propulsion Engineers
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• v.1 no.1
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• pp.104-110
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• 1997

To predict the effect of ring expansion and ring compression on residual stress relief of Al 7175 ring rot]s, 2-D axisymmetric thermal analysis and elastoplastic analysis were performed. The residual stress distributions along the thickness of T73, T7351 and T7352 treated rings were measured using three step sectioning method. The measured results were compared to numerical ones for quenched and stress relieved rings. After quenching, calculated hoop and axial residual stresses were similar to measured ones for T73 treated rings. The residual stresses of T7351 and T7352 treated rings were decreased remarkably compared to T73 treated rings. The effect of axial residual stress relief was superior to that of hoop one, and also ring compression to ring expansion. It was concluded that ring compression is advantageous over ring expansion in view of stress relief effect and practicality, and vice versa in view of dimensional control and press power.

• Steel and Composite Structures
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• v.33 no.4
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• pp.489-507
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• 2019

In order to obtain high bearing capacity and good ductility simultaneously, a structural column with hybrid normal and high strength steel (HNHSS) welded box section has been developed. Residual stress is an important factor that can influence the behaviour of a structural member in steel structures. Accordingly, the magnitudes and distributions of residual stresses in HNHSS welded box sections were investigated experimentally using the sectioning method. In this study, the following four box sections were tested: one normal strength steel (NSS) section, one high strength steel (HSS) section, and two HNHSS sections. Based on the experimental data from previous studies and the test results of this study, the effects of the width-to-thickness ratio of plate, yield strength of plate, and the plate thickness of the residual stresses of welded box sections were investigated in detail. A unified residual stress model for NSS, HSS and HNHSS welded box sections was proposed, and the corresponding simplified prediction equations for the maximum tensile residual stress ratio (${\sigma}_{rt}/f_y$) and average compressive residual stress ratio (${\sigma}_{rc}/f_y$) in the model were quantitatively established. The predicted magnitudes and distributions of residual stresses for four tested sections in this study by using the proposed residual stress model were compared with the experimental results, and the feasibility of this proposed model was shown to be in good agreement.

• Journal of the Korean Society of Propulsion Engineers
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• v.2 no.1
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• pp.31-40
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• 1998

To predict residual stresses of aluminum ring rolls after quenching and element removal, 2-D and 3-D thermal elasto-plastic analyses were performed. Strains measured by three step sectioning method were directly compared to those analysed using ABAQUS's element removal. Numerical residual stresses after quenching had similar tendency to measured ones after 2 step aging, but the difference between numerical and measured ones was large. The difference is the reason why there are nonuniform residual stress distributions to ring height direction due to small height of ring, It is judged that the increase of ring height will improve the accuracy of measured ones and decrease the difference. By direct comparison between 3-D numerical strains to simulate three step sectioning method and measured ones, the accuracy of measurement and analysis can be improved. It is concluded that there can be predicted the deformation behavior on machining complex shaped large structures with residual stresses.

• Journal of Welding and Joining
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• v.21 no.1
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• pp.66-71
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• 2003

The release of residual stress by mechanical loading and unloading is often performed in the fabrication of box structure fur steel bridge. The proper degree of loading and unloading is significant at release method of residual stress by mechanical loading because that degree is changed by material and geometric shape of welded structure. Therefore, the simulation model that could exactly analyze the release of residual stress by mechanical loading is to be necessary. In this study, the non-linear behavior of weldments under external loading and unloading, such as the decrease and increase of structure stiffness, was investigated by monitoring of nominal stress and strain. Tensile loading and unloading test and the proper degree of stress relaxation was measured by sectioning technique using strain gauge. Analysis model that is indispensable for the effective application of MSR method was established on the basis of test and measurement result.

• Journal of the Korean Society for Precision Engineering
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• v.19 no.9
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• pp.151-156
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• 2002

A new position correction method using four fiducials as reference points was developed and examined. It was aimed to calibrate nonlinear deformation by numerous error sources. A correlation for correction was derived from the geometric relationship between four fiducials and chip position. Compared with three points method, it exhibited more accurate correction, especially for inner area of a quadrilateral composed of four fiducial points. Its accuracy was found to be increased as fiducials moves outwardly within a printed circuit board (PCB) and/or as they form more rectangle-like shape As for arbitrarily nonlinear deformation, this method can be applied using more than five fiducials. In this case, local-area calibration is carried out by sectioning a board area into several rectangular are as.