• Horticultural Science & Technology
• /
• v.19 no.3
• /
• pp.334-337
• /
• 2001

This study was conducted to investigate the effect of highland (Daekwallyung, altitude of 800m above sea level) production of cucumber (Cucumis sativus L.) seedlings for retarding culture in lowland (Kangnung, altitude of 20m above sea level). Cucumbers (cv. Jangilbanbaek, Jeongsunyeoreum and Ginseollok) were raised during 25 days from 31st July to 25th August, and then planted under the rain-shelter at Kangnung 25th August. Mean and minimum temperature during raising seedlings at Daekwallyung, $20.9^{\circ}C$ and $16.1^{\circ}C$ were $3.7^{\circ}C$ and $5.3^{\circ}C$ lower than those of Kangnung, respectively. T/R ratio of the seedlings produced at Daekwallyung was lower than that of Kangnung, but chlorophyll contents were higher. Daekwallyung seedlings were higher female/male ratio compared with seedlings from Kangnung. Marketable yield from cucumber seedlings (cv. Jeongsunyeoreum) produced at Daekwallyung was 32% higher than that of Kangnung. These results showed that cucumber seedling raising place was Daekwallyung for the retarding culture during summer season, particularly, and the best cultivars was Baekchim (cv. Jeongsunyeoreum).

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.46 no.3
• /
• pp.374-380
• /
• 2017

Image analysis and k-mean clustering were conducted to develop a grading system of dried sea cucumber (SC) based on rehydration rate. The SC images were obtained by taking pictures in a box under controlled light conditions. The region of interest was extracted to depict the shape of the SC in a 2D graph, and those 2D shapes were rendered to build a 3D model. The results from the image analysis provided the morphological features of the SC, including length, width, surface area, and volume, to obtain the parameters of the k-mean clustering weight. The k-mean clustering classified the SC samples into three different grades. Each SC sample was rehydrated at $30^{\circ}C$ for 40 h. During rehydration, the flux of each grade was analyzed. Our study demonstrates that the mass transfer rate of SC increased as the surface area increased, and the grade of SC was classified based on rehydration rate. This study suggests that the optimal rehydration process for SC can be achieved by applying a suitable grading system.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.2
• /
• pp.338-348
• /
• 2017

Protein hydrolysates derived from plants and animals having antioxidant, suppression of hypertension, immunodulatory, alleviation of pain, and antimicrobial activity has been known as playing important role like hormone. This study was performed to optimize the hydrolysis of protein of sea of cucumber by a flavourzyme. The ranges of processes were the reaction temperature of 40 to $60^{\circ}C$, pH 6 to 8, and enzyme concentration 0.5 to 1.5%(w/v). As a result, the optimization of process was determined at temperature of $48-50^{\circ}C$, pH of 7.0-7.2, and enzyme concentration of 1.0-1.1%(w/v), and degree of hydrolysis was 43-45 at above conditions. The molecular weight of hydrolysate was distributed to 500-3,500 Da and showed typical peptides. Inhibition concentration ($IC_{50}$) of peptides of DPPH radical scavenging activity, Superoxide anion radical scavenging activity, Hydroxy radical scavenging activity, $Fe^{2+}$ cheating activity was 1.25, 3.40, 10.3, and 22.11 mg/mL, respectively. Therefore, we expect that those products are useful as functional food ingredients.

• Journal of Marine Life Science
• /
• v.7 no.1
• /
• pp.29-36
• /
• 2022

Sea cucumber, Aposticopus japonicus, is a major invertebrate species in the coastal regions of Korea. To evaluate the short-term stress levels according to the releasing methods, this study investigated the gene expression profiles of heat shock protein 90 (HSP90) by real-time quantitative polymerase chain reaction. When the juvenile sea cucumbers were packed in the vinyl bag with oxygen followed by transportation for 30 min or air-exposed for 1 h, the HSP90 gene expression levels in the experimental groups were significantly increased compared to those of the control groups (transported group, p=0.001; air-exposed group, p=0.032). The experimental group at 6 h post-release by seed-spreading method and at 2~6 h post-release by underwater hose-releasing method on board a fishing boat showed that the levels of HSP90 gene expression were not statistically significant but decreased slightly compared to the control group (seed-spreading group, p=0.069; hose-releasing group, p=0.093). On the other hand, the HSP90 gene expression showed an increasing pattern as the time passed (~6 h) after underwater release of juvenile sea cucumbers by divers (p=0.061). These results suggest that HSP90 gene expression can be used to investigate short-term stress response and effective releasing methods of juvenile sea cucumbers.

• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.2
• /
• pp.511-517
• /
• 2016

Scabraside D, a sulfated triterpene glycoside extract from sea cucumber Holothulia scabra, shows various biological activities, but effects on human cholangiocarcinoma cells have not previously been reported. In the present study, we investigated the activity of scabraside D against human cholangiocarcinoma (HuCCA) both in vitro and for tumor growth inhibition in vivo using a xenograft model in nude mice. Scabraside D ($12.5-100{\mu}g/mL$) significantly decreased the viability and the migration of the HuCCA cells in a dose-dependent manner, with 50% inhibitory concentration (IC50) of $12.8{\pm}0.05{\mu}g/mL$ at 24 h. It induced signs of apoptotic cells, including shrinkage, pyknosis and karyorrhetic nuclei and DNA fragmentation on agarose gel electrophoresis. Moreover, by quantitative real-time PCR, scabraside D effectively decreased Bcl-2 while increasing Bax and Caspase-3 gene expression levels suggesting that the scabraside D could induce apoptosis in HuCCA cells. In vivo study demonstrated that scabraside D (1 mg/kg/day, i.p. for 21 days) significantly reduced growth of the HuCCA xenografts without adverse effects on the nude mice. Conclusively, scabraside D induced apoptosis in HuCCA cells and reduced the growth of HuCCA xenographs model. Therefore, scabraside D may have potential as a new therapeutic agent for cholangiocarcinoma.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.6
• /
• pp.775-783
• /
• 2021

Sea cucumber, Holothuria scabra, is a well-known traditional Asian medicine that has been used for suppressing inflammation, promoting wound healing, and improving immunity. Moreover, previous studies demonstrated that the extract from H. scabra contains many bioactive compounds with potent inhibitory effect on tumor cell survival and progression. However, the effect of the methanolic extract from the body wall of H. scabra (BWMT) on human prostate cancer cells has not yet been investigated. In this study, we aimed to investigate the effects and underlying mechanism of BWMT on prostate cancer cell viability and metastasis. BWMT was obtained by maceration with methanol. The effect of BWMT on cell viability was assessed by MTT and colony formation assays. The intracellular ROS accumulation was evaluated using a DCFH-DA fluorescence probe. Hoechst 33342 staining and Annexin V-FITC/PI staining were used to examine the apoptotic-inducing effect of the extract. A transwell migration assay was performed to determine the anti-metastasis effect. BWMT significantly reduced cell viability and triggered cellular apoptosis by accumulating intracellular ROS resulting in the upregulation of JNK and p38 signaling pathways. In addition, BWMT also inhibited the invasion of PC3 cells by downregulating MMP-2/-9 expression via the ERK pathway. Consequently, our study provides BWMT from H. scabra as a putative therapeutic agent that could be applicable against prostate cancer progression.

• Fisheries and Aquatic Sciences
• /
• v.24 no.8
• /
• pp.284-295
• /
• 2021

Red sea cucumber Stichopus japonicus is an invertebrate animal inhabiting in coasts of Korea, China, and Japan. They are traditionally used for food and medicine and well known for their distinctive biologically and pharmacologically important compounds. We investigated the effect of amyloglucosidase (AMG) enzymatic extracts of S. japonicus (AESJ) on the proliferation and cytokine secretion of murine splenocytes stimulated with concanavalin A (Con A). AESJ enhanced the proliferation of splenocytes and the production of IL-2 (Th1 cytokine), IL-1β (Th1 promoting cytokine), and IL-4, IL-10 (Th2 type cytokines) when treated alone. However, under Con A stimulation, AESJ suppressed the proliferation of splenocytes, attenuated the secretion of IL-2, IL-4, IL-10, and enhanced IL-1β secretion. These results suggest that AESJ exhibits immunomodulatory effect by moderating the proliferation of splenocytes and the secretion of IL-2, IL-1β, IL-4, and IL-10 differently depending on the absence and presence of Con A stimulation. These data evidence the immunomodulatory potential of AESJ, which can be further developed into a functional food mediating homeostasis.

• Journal of the Korean Institute of Rural Architecture
• /
• v.24 no.4
• /
• pp.125-132
• /
• 2022

It can be seen that Traditional house of Jeong Si-yeong is located in a place where Saenggi(good energy), which is important in Pungsu(Feng Shui), can be gathered and that energy can be properly maintained. According to the theory of feng shui, a place that is not easily exposed by the surrounding mountains and is well protected by the strong wind was selected, and the main room was placed on the south side so that the sunlight was adequate while facing the north, so that you can live a comfortable life for a long time. Located on such a relatively well-hidden site, it is a location that can cope well with the invasion of Japanese invaders through the sea in the past, and even today, it is seen as a reasonable base that can be properly protected from strong sea winds in reality. On the other hand, if we look at the Hyungguk theory, it was a house built in the late Joseon Dynasty, and we could see the hidden hopes of the nobles at the time. The mountain behind the house is a haebok-type with a crab lying on the seashore, and what the crab symbolizes is the past national exam for official. Considering that the name of the place where the house is located is also Oyat(cucumber tree)-ri, where many cucumber trees closely related to the royal family of the Joseon Dynasty were planted, it seems that the family wished for prosperity by producing many Sadaebu(upper class gentry) in the past and forming a good relationship with the royal family.

• Journal of the Korean Society of Food Culture
• /
• v.27 no.5
• /
• pp.469-480
• /
• 2012

Since its introduction in"Jeungbosallimgyeongje" in the mid-1700s, Chogyetang has continuously changed as a cooked food while still reflecting the era as in the following four phases. In the first phase from 1766 to 1920, Chogyetang was served as a hot pot dish consisting of boiled chicken with spring onions, vinegar, soy sauce, oil, and egg. The second phase from 1930 to 1950 involved the removal of vinegar, an important seasoning. Instead, a wider variety of materials such as beef, sea cucumber, abalone, cucumber, and mushroom were added. Third, from the late-1950s to 1980s, there were significant changes both in the materials and recipe. Chogyetang was changed into a cold food for consumption in the summer in which sesame, a new material, was added to make soup. The prepared soup was then poured over the chicken and vegetables. Fourth, from the late-1980s to the present, sesame, the main ingredient added in the third phase, was removed. Instead, vinegar, mustard, and sugar were added in order to increase taste. Therefore, Chogyetang has been changed into an a la carte menu item in which vegetables and noodles are added to boiled chicken, and it has become a popular summer food consumed when eating-out.

• Korean Journal of Microbiology
• /
• v.49 no.3
• /
• pp.237-244
• /
• 2013

Bacterial diversities in the guts of sea cucumbers (Apostichopus japonicus) and shrimps (Litopenaeus vannamei) were investigated using barcoded or tag-encoded 454 pyrosequencing of 16S rRNA genes. In sea cucumbers, most of sequences were related to two genera, the genus Propionigenium in the phylum Fusobacteria and an unclassified genus in the family Flavobacteriaceae of phylum Bacteroidetes. Shrimps showed various kinds of genera including Lactococcus, Leuconostoc, Prochlorococcus, and Vibrio as well as the unclassified genera in the families, Flavobacteriaceae, Rhodobacteraceae, Desulfobulbaceae, and Helicobacteraceae and in the order Mycoplasmatales. Unclassified genera containing environmental sequences only are more than half of genera from sea cucumbers and shrimps. Sea cucumbers and shrimps could be unexplored sources of novel microbes and the bacterial diversity of them was revealed by high throughput 454 pyrosequencing.