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http://dx.doi.org/10.23005/ksmls.2022.7.1.29

Heat Shock Protein 90 Gene Expression in Juvenile Sea Cucumber Apostichopus japonicus (Echinodermata; Holothuroidea) according to Releasing Methods  

Lee, Dong-Han (Department of Marine Bioscience, Gangneung-Wonju National University)
Lee, Seungheon (Department of Marine Bioscience, Gangneung-Wonju National University)
Jeong, Dong-Bin (Department of Information Statistics, Gangneung-Wonju National University)
Sohn, Young Chang (Department of Marine Bioscience, Gangneung-Wonju National University)
Publication Information
Journal of Marine Life Science / v.7, no.1, 2022 , pp. 29-36 More about this Journal
Abstract
Sea cucumber, Aposticopus japonicus, is a major invertebrate species in the coastal regions of Korea. To evaluate the short-term stress levels according to the releasing methods, this study investigated the gene expression profiles of heat shock protein 90 (HSP90) by real-time quantitative polymerase chain reaction. When the juvenile sea cucumbers were packed in the vinyl bag with oxygen followed by transportation for 30 min or air-exposed for 1 h, the HSP90 gene expression levels in the experimental groups were significantly increased compared to those of the control groups (transported group, p=0.001; air-exposed group, p=0.032). The experimental group at 6 h post-release by seed-spreading method and at 2~6 h post-release by underwater hose-releasing method on board a fishing boat showed that the levels of HSP90 gene expression were not statistically significant but decreased slightly compared to the control group (seed-spreading group, p=0.069; hose-releasing group, p=0.093). On the other hand, the HSP90 gene expression showed an increasing pattern as the time passed (~6 h) after underwater release of juvenile sea cucumbers by divers (p=0.061). These results suggest that HSP90 gene expression can be used to investigate short-term stress response and effective releasing methods of juvenile sea cucumbers.
Keywords
Apostichopus japonicus; Releasing methods; RT-qPCR;
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