• Title/Summary/Keyword: science and technology studies

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Comparing Protein Expression in Erwinia amylovora Strain TS3128 Cultured under Three Sets of Environmental Conditions

  • Lee, Jongchan;Choi, Junhyeok;Lee, Jeongwook;Cho, Yongmin;Kang, In-Jeong;Han, Sang-Wook
    • The Plant Pathology Journal
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    • v.38 no.4
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    • pp.410-416
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    • 2022
  • Erwinia amylovora, the causal agent of fire-blight disease in apple and pear trees, was first isolated in South Korea in 2015. Although numerous studies, including omics analyses, have been conducted on other strains of E. amylovora, studies on South Korean isolates remain limited. In this study, we conducted a comparative proteomic analysis of the strain TS3128, cultured in three media representing different growth conditions. Proteins related to virulence, type III secretion system, and amylovoran production, were more abundant under minimal conditions than in rich conditions. Additionally, various proteins associated with energy production, carbohydrate metabolism, cell wall/membrane/envelope biogenesis, and ion uptake were identified under minimal conditions. The strain TS3128 expresses these proteins to survive in harsh environments. These findings contribute to understanding the cellular mechanisms driving its adaptations to different environmental conditions and provide proteome profiles as reference for future studies on the virulence and adaptation mechanisms of South Korean strains.

Chronic Toxicity of the Triazole Fungicide Tebuconazole on a Heterocystous, Nitrogen-Fixing Rice Paddy Field Cyanobacterium, Westiellopsis prolifica Janet

  • Nirmal Kumar, J.I.;Bora, Anubhuti;Amb, Manmeet Kaur
    • Journal of Microbiology and Biotechnology
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    • v.20 no.7
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    • pp.1134-1139
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    • 2010
  • This study explored the chronic effects of different doses of the triazole fungicide tebuconazole on the growth, and metabolic and enzymatic functions of the filamentous paddy field cyanobacterium, Westiellopsis prolifica Janet. The growth of the cyanobacterium was determined by an estimation of the change in pigment contents. Chlorophyll-a, carotenoids, and accessory pigments such as phycocyanin, allophycocyanin, and phycoerythrin were shown to decline over a 16-day period by a factor of 92%, 93%, 83%, 95%, and 100%, respectively, with increasing doses of the fungicide. Metabolic and enzymatic activities were also adversely affected. Over the 16 days, a gradual rise in total phenol content was recorded when Westiellopsis prolifica Janet was treated with 60 ppm of the fungicide, despite the reduction in carbohydrates, proteins, and amino acids by 96%, 92%, and 90%, respectively. Moreover, the enzymes nitrate reductase (NR), glutamine synthetase (GS), and succinate dehydrogenase (SDH) also registered reductions of 93%, 90%, and 98%, respectively. This study indicates that tebuconazole, although an important fungicide used extensively in rice fields, exhibits an inhibitory effect on the growth and metabolic activities of Westiellopsis prolifica Janet and hence possibly on other varieties as well.

INTERLAYER COUPLING AND MAGNETOOPTICS IN MULTILAYERS

  • Lu, M.;Bie, Q.S.;Xu, Y.B.;Zhai, H.R.;Zhou, S.M.;Chen, Liangyao;Jin, Q.Y.
    • Journal of the Korean Magnetics Society
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    • v.5 no.5
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    • pp.561-566
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    • 1995
  • Additional magnetooptical Kerr effect (AMOKE) was observed in several multilayer structures. For Fe/Pd and Co/Cu Multilayers, AMOKE enhanced the Kerr rotation in short wavelength side, while for Fe/Ag and FeSi/Cu multilayer systems the Kerr rotation enhancement appeared in long wavelength side. A number of ferromagnetic/nonmagnetic/ferromagnetic(FM/NM/FM) sandwiches showed that the AMOKE led to oscillations of Kerr rotation and Kerr ellipticity in certain wavelength range with changing NM layer thickness similar to the oscillatory interlayer coupling. The oscillation of effective optical constants related to the MOKE oscillation was observed for the first time. The mechanisms of the AMOKE were discussed.

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Multi-class Analysis of Exposure Chemicals in Deciduous Teeth by Liquid Chromatography-Mass Spectrometry: Preliminary Studies on Sample Preparation Methods

  • Lee, Yujin;Seo, Eunji;Park, Jun Young;Bae, Kwang-Hak;Lee, Jaeick;Cha, Sangwon
    • Mass Spectrometry Letters
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    • v.9 no.4
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    • pp.110-114
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    • 2018
  • Since accumulation of chemicals in deciduous teeth can occur from the second trimester of fetal development to shedding, a deciduous tooth has been considered as an attractive biomatrix for estimating individual chemical exposures recently. Therefore, detection of organic chemicals from teeth has received an increasing attention in exposomics research. Most previous studies on organic chemical analysis of teeth not only focused on a few targeted chemicals but also ignored potential contaminants from an enamel surface or a dental pulp. Recently, our group started developing a multi-class organic analysis method for deciduous teeth and tried to find a proper incubation condition of tooth materials. Our results showed that incubation with methanolic HCl provided the best performance among tested.

Prognostic Significance of C-reactive Protein in Urological Cancers: a Systematic Review and Meta-analysis

  • Dai, Jin;Tang, Kun;Xiao, Wei;Yu, Gan;Zeng, Jin;Li, Wei;Zhang, Ya-Qun;Xu, Hua;Chen, Zhi-Qiang;Ye, Zhang-Qun
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.8
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    • pp.3369-3375
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    • 2014
  • Background: C-reactive protein (CRP), considered as a prototypical inflammatory cytokine, has been proposed to be involved in tumor progression through inflammation. Recent studies have indicated CRP as a progostic predictor for urological cancers, but the results remain controversial. Materials and Methods: A systematic search of Medline, Scopus and the Cochrane Library was performed to identify eligible studies published between Jan 1, 2001 and Sep 1, 2013. Outcomes of interest were collected from studies comparing overall survival (OS), cancer-specific survival (CSS) and relapse-free survival (RFS) in patients with elevated CRP levels and those having lower levels. Studies were pooled, and combined hazard ratio (HR) of CRP with its 95% confidence interval (CI) for survival were used for the effect size estimate. Results: A total of 43 studies (7,490 patients) were included in this meta-analysis (25 for RCC, 10 for UC, and 8 for PC). Our pooled results showed that elevated serum CRP level was associated with poor OS (HR: 1.26, 95%CI: 1.22-1.30) and RFS (HR: 1.38 95%CI: 1.29-1.47), respectively. For CSS the pooled HR (HR: 1.33, 95%CI: 1.28-1.39) for higher CRP expression could strongly predict poorer survival in urological cancers. Simultaneously, elevated serum CRP was also significantly associated with poor prognosis in the subgroup analysis. Conclusions: Our pooled results demonstrate that a high serum level of CRP as an inflammation biomarker denotes a poor prognosis of patients with urological cancers. Further large prospective studies should be performed to confirm whether CRP, as a biomarker of inflammation, has a prognostic role in urological cancer progression.

Doing Science, Technology, and Women Studies : "With the Body" in-between New Material Feminism and STS (과학기술과 여성 연구하기: 신유물론 페미니즘과 과학기술학의 안-사이에서 "몸과 함께")

  • Leem, So Yeon
    • Journal of Science and Technology Studies
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    • v.19 no.3
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    • pp.167-200
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    • 2019
  • In recent years, the material has mattered to the humanities and social sciences in the West and feminism is no exception. It is a notable change that feminist analyses, which had previously shown critiques against science and technology, attempted to build new engagements with matter. STS (science and technology studies) has also accumulated scholarships and developed conceptual tools on the thing or the existence itself through "the ontological turn." The objective of this paper is not to introduce new materialism or new material feminism in full, but to reveal the possibility and potential of doing science, technology and women studies by selectively relying on the achievement of new materialism feminism. This article shows a way to study women's practice of science and technology by analyzing the case of plastic surgery practices through the ontological concepts of STS, particularly those of Annemarie Mol, Karen Barad, and Charis Thompson, and proposes a new engagement among new materialism, feminism, and STS. This article is organized as follows. First of all, after briefly discussing main issues in new material feminism, I will show the limitations of previous feminist studies of plastic surgery under the light of new material feminism. The rest of the article introduces the conceptual tools of ontological STS, describes plastic surgery practices with those tools, and finally provides their feminist implications.

Information Verification Practices and Perception of Social Media Users on Fact-Checking Services

  • Rabby Q., Lavilles;January F., Naga;Mia Amor C., Tinam-isan;Julieto E., Perez;Eddie Bouy B., Palad
    • Journal of Information Science Theory and Practice
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    • v.11 no.1
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    • pp.1-13
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    • 2023
  • This study determines how social media users (SMUs) verify the information they come across on the Internet. It determines SMUs' perception of online fact-checking services in terms of their ease of use, usefulness, and trust. By conducting a focus group discussion and key informant interviews, themes were derived in determining fact-checking practices while a survey was further conducted to determine such perceived ease of use, usefulness, and trust in fact-checking services. The thematic analysis revealed major information verification practices, such as cross-checking and verifying with other sources, inspecting comments and reactions, and confirming from personal and social networks. The results showed that SMUs considered fact-checking services easy to use. However, a concern was raised about their usefulness stemming from the delayed action in addressing the information issues that need to be verified. As to perceived trust, it was found that SMUs have reservations about fact-checking services. Finally, it is believed that fact-checking services are expected to be credible and need to be promoted to mitigate any form of fake news, particularly on social media platforms.

The MTHFR C677T Polymorphism and Risk of Acute Lymphoblastic Leukemia: an Updated Meta-analysis Based on 37 Case-control Studies

  • Jiang, Yuan;Hou, Jing;Zhang, Qiang;Jia, Shu-Ting;Wang, Bo-Yuan;Zhang, Ji-Hong;Tang, Wen-Ru;Luo, Ying
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.11
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    • pp.6357-6362
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    • 2013
  • Background: The C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) has been associated with acute lymphoblastic leukemia (ALL). However, results were conflicting. The aim of this study was to quantitatively summarize the evidence for the MTHFRC677T polymorphism and ALL risk. Methods: Electronic searches of PubMed and the Chinese Biomedicine database were conducted to select case-control studies containing available genotype frequencies of C677T and the odds ratio (OR) with 95% confidence interval (CI) was used to assess the strength of any association. Results: Case-control studies including 6,371 cases and 10,850 controls were identified. The meta-analysis stratified by ethnicity showed that individuals with the homozygous TT genotype had decreased risk of ALL (OR= 0.776, 95% CI: 0.687~0.877, p< 0.001) in Caucasians (OR= 0.715, 95% CI: 0.655~0.781, p= 0.000). However, results among Asians (OR=0.711, 95% CI: 0.591~1.005, p= 0.055) and others (OR=0.913, 95% CI: 0.656~1.271, p= 0. 590) did not suggest an association. A symmetric funnel plot, the Egger's test (P=0.093), and the Begg- test (P=0.072) were all suggestive of the lack of publication bias. Conclusion: This meta-analysis supports the idea that the MTHFR C677T genotype is associated with risk of ALL in Caucasians. To draw comprehensive and true conclusions, further prospective studies with larger numbers of participants worldwide are needed to examine associations between the MTHFRC677T polymorphism and ALL.

Optimization of modal load pattern for pushover analysis of building structures

  • Shayanfar, Mohsen Ali;Ashoory, Mansoor;Bakhshpoori, Taha;Farhadi, Basir
    • Structural Engineering and Mechanics
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    • v.47 no.1
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    • pp.119-129
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    • 2013
  • Nonlinear Static Procedures (NSPs) have been developed as a practical tool to estimate the seismic demand of structures. Several researches have accomplished to minimize errors of NSPs, namely pushover procedures, in the Nonlinear Time History Analysis (NTHA), as the most exact method. The most important issue in a typical pushover procedure is the pattern and technique of loading which are extracted based on structural dynamic fundamentals. In this paper, the coefficients of modal force combination is focused involving a meta-heuristic optimization algorithm to find the optimum load pattern which results in a response with minimum amount of errors in comparison to the NTHA counterpart. Other parameters of the problem are based on the FEMA recommendations for pushover analysis of building structures. The proposed approach is implemented on a high-rise 20 storey concrete moment resisting frame under three earthquake records. In order to demonstrate the effectiveness and robustness of the studied procedure the results are presented beside other well-known pushover methods such as MPA and the FEMA procedures, and the results show the efficiency of the proposed load patterns.