Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Multi-class Analysis of Exposure Chemicals in Deciduous Teeth by Liquid Chromatography-Mass Spectrometry: Preliminary Studies on Sample Preparation Methods

  • Lee, Yujin (Department of Chemistry, Hankuk University of Foreign Studies) ;
  • Seo, Eunji (Department of Chemistry, Hankuk University of Foreign Studies) ;
  • Park, Jun Young (Department of Chemistry, Hankuk University of Foreign Studies) ;
  • Bae, Kwang-Hak (Oral Health Science Research Center, Apple tree Dental Hospital) ;
  • Lee, Jaeick (Analytical Science and Technology Laboratory, Doping Control Center, Korea Institute of Science and Technology) ;
  • Cha, Sangwon (Department of Chemistry, Hankuk University of Foreign Studies)
  • Received : 2018.12.17
  • Accepted : 2018.12.18
  • Published : 2018.12.30
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Abstract

Since accumulation of chemicals in deciduous teeth can occur from the second trimester of fetal development to shedding, a deciduous tooth has been considered as an attractive biomatrix for estimating individual chemical exposures recently. Therefore, detection of organic chemicals from teeth has received an increasing attention in exposomics research. Most previous studies on organic chemical analysis of teeth not only focused on a few targeted chemicals but also ignored potential contaminants from an enamel surface or a dental pulp. Recently, our group started developing a multi-class organic analysis method for deciduous teeth and tried to find a proper incubation condition of tooth materials. Our results showed that incubation with methanolic HCl provided the best performance among tested.

Keywords

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Figure 1. Experimental workflow for analysis of organic chemicals present in deciduous teeth.

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Figure 2. MALDI mass spectra of (a) the first washing solution and (b) the DCM washing solution. Peaks with asterisks (*) are DHB matrix-originated ion signals.

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Figure 3. Chemical structures of organic chemical compounds detected from a pooled deciduous teeth powder.

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Figure 4. (a) Extracted ion chromatograms (EICs) of major compounds detected from a pooled teeth powder which was incubated in 70% (v/v) methanolic HCl. (b) EICs of corresponding compounds present in a positive control.

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