• 제목/요약/키워드: sample enrichment

검색결과 121건 처리시간 0.021초

초등학생 자녀를 둔 취업모의 일-가족·양육 갈등 및 향상 유형: 다양한 예측 요인과 심리적 결과 (Profiles of Work-Family/Parenting Conflict and Enrichment Among Korean Employed Mothers of Children in Elementary School: Various Antecedents and Psychological Outcomes)

  • 박인숙;이재림
    • 가족자원경영과 정책
    • /
    • 제26권2호
    • /
    • pp.19-36
    • /
    • 2022
  • 본 연구의 목적은 (1) 초등학교 3학년 자녀를 둔 취업모의 일-가족 갈등, 일-가족 향상, 일-양육 갈등, 일-양육 향상을 분류지표로 하는 유형을 도출하고, (2) 각 유형을 예측하는 개인, 가족, 직장, 지역사회 특성을 규명하며, (3) 유형별로 심리적 결과에 차이가 있는지를 살펴보는 것이다. 이를 위해, 한국아동패널 10차년도(2017년, 초등학교 3학년 시기) 보호자용과 어머니용 자료 중 기혼이면서 상용직으로 근무하고 있는 여성 451명의 응답을 사용하였다. 잠재프로파일분석 결과, 일-가족·양육 (1) 향상형(11.91%), (2) 중간형(47.85%), (3) 혼재형(40.24%)의 세 가지 유형으로 구분되었다. 유형분류를 예측하는 요인은 주관적 건강상태, 자녀의 학교적응, 근무시간, 양육환경 적절성 및 지역사회 서비스인프라 만족도였다. 유형별 심리적 결과는 삶의 만족도, 결혼만족도, 직장만족도의 경우 향상형, 중간형, 혼재형의 순서였고, 세 집단의 차이가 유의하였다. 우울은 혼재형, 중간형, 향상형의 순서였고, 역시 세 집단의 차이가 유의하였다. 본 연구는 일-가족 갈등과 향상, 일-양육 갈등과 향상을 기반으로 발견한 유형별 차이를 알아봄에 있어 다양한 예측요인과 심리적 결과를 살펴봄으로써, 일-가족·양육 유형화에 관한 종합적인 이해를 도모했다는 의의가 있다.

Headspace Hanging Drop Liquid Phase Microextraction and GC-MS for the Determination of Linalool from Evening Primrose Flowers

  • Kim, Nam-Sun;Jung, Mi-Jin;Yoo, Zoo-Won;Lee, Sun-Neo;Lee, Dong-Sun
    • Bulletin of the Korean Chemical Society
    • /
    • 제26권12호
    • /
    • pp.1996-2000
    • /
    • 2005
  • Headspace hanging drop liquid phase micro-extraction (HS-HD-LPME) is studied as a novel solvent-based sample pretreatment method for floral volatile aroma compounds. This paper reports on application of the HSHD- LPME combined with GC-MS for the analysis of linalool component emitted from evening primrose flowers. The effect of several variables on the method performance was investigated. Additionally, the separation of enantiomers on a cyclodextrin capillary column was performed to identify chirality of (−)-linalool component. Since the unsurpassed volume of a few micro-liters of solvent is used, there is minimal waste or exposure to toxic organic solvents. This method enables to combine extraction, enrichment, clean-up, and sample introduction into a single step prior to the chromatographic process.

Sorbent Extraction of Some Metal Ions on a Gas Chromatographic Stationary Phase Prior to Their Flame Atomic Absorption Determinations

  • Soylak, M.;Saracoglu, S.;Elci, L.
    • Bulletin of the Korean Chemical Society
    • /
    • 제24권5호
    • /
    • pp.555-558
    • /
    • 2003
  • An enrichment/separation system for atomic absorption spectrometric determinations of Cu(Ⅱ), Fe(Ⅲ), Ni(Ⅱ) and Co(Ⅱ) has been established. The procedure is based on the adsorption of the analytes as calmagite chelates on Chromosorb-102. The effects of some parameters including pH, amount of ligand, salt matrix, flow rates of sample and eluent solutions were investigated. Under optimized conditions, the relative standard deviation of the combined method of sample treatment, preconcentration and determination with FAAS (N=5) is generally lower than 5%. The limit of detection (3σ) was between 6.0-112.9 ㎍/L. The results were used for preconcentration of analytes from some sodium and ammonium salt.

어패류에서 분리한 장염비브리오균의 분포 및 항균제 감수성 (Antibiotic Susceptibility and Distribution of Vibrio parahaemolyticus Isolated from the Seafood)

  • 이향;오영희;최성민;박석기
    • 한국환경보건학회지
    • /
    • 제33권1호
    • /
    • pp.16-20
    • /
    • 2007
  • Vibrio parahaemolyticus was investigated for analysis of distribution and antibiotic susceptibility at the markets, Seoul in 2004. The average isolation rate was 26.8%(306/1,143) and the highest rate with 64.9% was obtained in August. The isolation rates from December to April was 0.0%. It is more effective to inoculate into the enrichment medium, alkaline pepton water by swabbing a sample with a cotton ball rather than pouring mixed sample. The isolation rates were from 24.4% to 37.1% in domestic samples and from 9.8% to 26.8% in imported ones. All of the isolates were resistent to ampicillin and ticarcillin and most of them were sensitive to chloramphenicol, tetracycline, nalidixic acid, amoxicillin, ceftriaxone, streptomycin, kanamycin, trimethoprim/sulfamethoxazol, ampicillin/sulbactam, ciprofloxacin.

Towards inferring reactor operations from high-level waste

  • Benjamin Jung;Antonio Figueroa;Malte Gottsche
    • Nuclear Engineering and Technology
    • /
    • 제56권7호
    • /
    • pp.2704-2710
    • /
    • 2024
  • Nuclear archaeology research provides scientific methods to reconstruct the operating histories of fissile material production facilities to account for past fissile material production. While it has typically focused on analyzing material in permanent reactor structures, spent fuel or high-level waste also hold information about the reactor operation. In this computational study, we explore a Bayesian inference framework for reconstructing the operational history from measurements of isotope ratios from a sample of nuclear waste. We investigate two different inference models. The first model discriminates between three potential reactors of origin (Magnox, PWR, and PHWR) while simultaneously reconstructing the fuel burnup, time since irradiation, initial enrichment, and average power density. The second model reconstructs the fuel burnup and time since irradiation of two batches of waste in a mixed sample. Each of the models is applied to a set of simulated test data, and the performance is evaluated by comparing the highest posterior density regions to the corresponding parameter values of the test dataset. Both models perform well on the simulated test cases, which highlights the potential of the Bayesian inference framework and opens up avenues for further investigation.

MiRNA Synergistic Network Construction and Enrichment Analysis for Common Target Genes in Small-cell Lung Cancer

  • Zhang, Tie-Feng;Cheng, Ke-Wen;Shi, Wei-Yin;Zhang, Jin-Tao;Liu, Ke-Di;Xu, Shu-Guang;Chen, Ji-Quan
    • Asian Pacific Journal of Cancer Prevention
    • /
    • 제13권12호
    • /
    • pp.6375-6378
    • /
    • 2012
  • Background: Small-cell lung cancer (also known as SCLC) is an aggressive form and untreated patients generally die within about 3 months. To obtain further insight into mechanism underlying malignancy with this cancer, an miRNA synergistic regulatory network was constructed and analyzed in the present study. Method: A miRNA microarray dataset was downloaded from the NCBI GEO database (GSE27435). A total of 546 miRNAs were identified to be expressed in SCLC cells. Then a miRNA synergistic network was constructed, and the included miRNAs mapped to the network. Topology analysis was also performed to analyze the properties of the synergistic network. Consequently, we could identified constitutive modules. Further, common target genes of each module were identified with CFinder. Finally, enrichment analysis was performed for target genes. Results: In this study, a miRNA synergistic network with 464 miRNAs and 2981 edges was constructed. According to the topology analysis, the topological properties between the networks constructed by LC related miRNAs and LC unrelated miRNAs were significantly different. Moreover, a module cilque0 could be identified in our network using CFinder. The module included three miRNAs (hsa-let-7c, hsa-let-7b and hsa-let-7d). In addition, several genes were found which were predicted to be common targets of cilque0. The enrichment analysis demonstrated that these target genes were enriched in MAPK signaling pathways. Conclusions: Although limitations exist in the current data, the results uncovered here are important for understanding the key roles of miRNAs in SCLC. However, further validation is required since our results were based on microarray data derived from a small sample size.

Determination of Boron Isotopic Ratio by Using an Alpha Track Technique

  • Park, Yong-Joon;Pyo, Hyung-Yeal;Song, Kyu-Seok;Song, Byoung-Chul;Jee, Kwang-Yong;Kim, Won-Ho
    • Bulletin of the Korean Chemical Society
    • /
    • 제27권10호
    • /
    • pp.1609-1612
    • /
    • 2006
  • The determination of the boron isotopic ratio in solutions was achieved by means of a solid state track detector by using an alpha track. The neutron flux was optimized by using a Cd-foil to find the optimum conditions for counting the number of alpha tracks on the selected solid detector caused by the (n, $\alpha$) nuclear reaction of boron. The home-made multi-dot detector plate was utilized in this study to increase the reproducibility of the measurement by uniformly drying the boron solution within the marked circle area on the detector plate. The experimental results of this study verified that the $^{11}B/^{10}B $ isotopic ratio can be measured by observing the number of alpha tracks for different concentrated standard solutions with various isotopic compositions. This technique was applied to the determination of $^{10}B$ enrichment factor in a biological sample for a boron neutron capture therapy.

중합효소연쇄반응을 이용한 실험적 리스테리아 감염증의 신속진단 (Rapid diagnosis of experimental listeriosis in mice by polymerase chain reaction)

  • 강호조;이성미;석주명;이덕규;손원근
    • 대한수의학회지
    • /
    • 제38권3호
    • /
    • pp.559-564
    • /
    • 1998
  • The polymerase chain reaction(PCR) assay was used for rapid diagnosis from blood and organ samples experimentally infected with Listeria monocytogenes. This method used a pair of primers based on a unique region in the 16S rRNA sequence of L monocytogenes. Procedure A was based on dilution of the blood sample followed by lysis of bacterial cell and direct analysis of the lysate with PCR. In artificially infected blood samples with L monocytogenes, it was possible to detect fewer than 40 cells per ml of blood. However, L monocytogenes was detected low rates on infected organs by the direct PCR. In procedure B, enrichment cultivation was used to increase numbers of bacteria before lysis and PCR. L monocytogenes was detected from 23 samples of 24 liver and spleen, respectively, and 18 samples of 24 blood were found to be positive by PCR on a subset of 72 organ samples, whereas L monocytogenes were detected on 63 organ samples in classical culture technique. It was required to analyze including enrichment steps were 6h and 18h on the procedure A and B, respectively.

  • PDF

Enrichment of Peptides using Novel C8-functionalized Magnetic Nanoparticles for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometric Analysis

  • Song, Sun-Mi;Yang, Hyo-Jik;Kim, Jin-Hee;Shin, Seong-Jae;Park, Eun-Hye;Kim, Jeong-Kwon
    • Mass Spectrometry Letters
    • /
    • 제2권2호
    • /
    • pp.53-56
    • /
    • 2011
  • [ $C_8$ ]functionalized magnetic nanoparticles were synthesized by coating magnetic $Fe_3O_4$ nanoparticles with silicaamine groups using 3-aminopropyltriethoxysilane and by subsequently modifying the amine groups with chloro(dimethyl)octylsilane to produce octyl groups on the surface of the MNPs. The $C_8$-functionalized MNPs were used to enrich peptides from tryptic protein digests of myoglobin and ${\alpha}$-casein. The enriched peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). MALDI-MS was also used to investigate desalting of the $C_8$-functionalized MNPs. Sample solutions were prepared in 1.0 M NaCl, and the successful removal of salt was observed. Enrichment with $C_8$-functionalized MNPs was very effective for separating and concentrating tryptic peptides.

THE CHEMICAL PROPERTIES OF PG QUASARS

  • 신재진;우종학
    • 천문학회보
    • /
    • 제37권2호
    • /
    • pp.74.2-74.2
    • /
    • 2012
  • Metallicity is an important tracer of star formation in galaxy evolution. Based on the flux ratios of broad emission lines, AGN metallicity has shown a correlation with AGN luminosity. However, it is not clear what physical parameter drives the observed correlation. Using a sample 69 Palomar-Green QSOs at low-z (z<0.5), we determine BLR gas metallicity from emission line flux ratios, i.e., N V1240/C IV1549, (Si IV1398+O IV1402)/C IV1549 and N V1240/He II1640 based on the UV spectra from the HST and IUE archives. We compare BLR gas metallicity with various AGN properties, i.e., black hole mass, AGN luminosity and Eddington ratio, in order to investigate physical connection between metal enrichment and AGN activity. In contrast to high-z QSOs, which show the correlation between metallicity and black hole mass, we find that the metallicity of low-z QSOs correlates with Eddington ratio, but not with black hole mass, suggesting that metallicity enrichment mechanism is different between low-z and high-z.

  • PDF