• Title/Summary/Keyword: salt level

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Putrescine Transport in a Cyanobacterium Synechocystis sp. PCC 6803

  • Raksajit, Wuttinun;Maenpaa, Pirkko;Incharoensakdi, Aran
    • BMB Reports
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    • v.39 no.4
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    • pp.394-399
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    • 2006
  • The transport of putrescine into a moderately salt tolerant cyanobacterium Synechocystis sp. PCC 6803 was characterized by measuring the uptake of radioactively-labeled putrescine. Putrescine transport showed saturation kinetics with an apparent $K_m$ of $92{\pm}10\;{\mu}M$ and $V_{max}$ of $0.33{\pm}0.05\;nmol/min/mg$ protein. The transport of putrescine was pH-dependent with highest activity at pH 7.0. Strong inhibition of putrescine transport was caused by spermine and spermidine whereas only slight inhibition was observed by the addition of various amino acids. These results suggest that the transport system in Synechocystis sp. PCC 6803 is highly specific for polyamines. Putrescine transport is energy-dependent as evidenced by the inhibition by various metabolic inhibitors and ionophores. Slow growth was observed in cells grown under salt stress. Addition of low concentration of putrescine could restore growth almost to the level observed in the absence of salt stress. Upshift of the external osmolality generated by either NaCl or sorbitol caused an increased putrescine transport with an optimum 2-fold increase at 20 mosmol/kg. The stimulation of putrescine transport mediated by osmotic upshift was abolished in chloramphenicol-treated cells, suggesting possible involvement of an inducible transport system.

Physicochemical Characteristics of Toha-Jeot Added Cabbage Kimchi during Fermentation (토하젓 첨가 배추김치의 숙성 중 이화학적 특성)

  • 박영희;정난희;이성숙
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.3
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    • pp.426-431
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    • 2001
  • We made the Toha-jeot added cabbage kimchi with Toha-jeot, which is salt-fermented Toha shrimp in Korean traditional fermented food. The physicochemical characteristics of cabbage kimchi for 5 week fermentation at 4$^{\circ}C$ were observed. The salt content was 2.10~1.50% during the fermentation period. The salt content decreased rapidly during the first week, stayed the same level until the 4 week and then decreased after the 4 week. The pH of control kimchi and Toha-jeot added cabbage kimchi were 5.69, 5,64, respectively on preparation day. The rate of decrease of pH of Toha-jeot added cabbage kimchi was slow as the fermentation processed. The total acidity of the two kinds of kimchi was increased. The total acidity was higher from Toha-jeot added cabbage kimchi than control cabbage kimchi at the later stage of fermentation. The contents of total sugar and reducing sugar were higher from Toha-jeot added cabbage kimchi than control cabbage kimchi at the early stage of fermentation. The total sugar and reducing sugar contents of the two kinds of cabbage kimchi were decreased gradually as the fermentation processed. The ascorbic acid contents of the two kinds of kimchi were decreased slightly at the early stage of fermentation and then increased from 2 to 4 week, while there was no further change at 5 week.

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A Study on the Combined Deterioration of Concrete subjected to Freezing-Thawing and Chloride Attack (동해와 염해를 동시에 받는 콘크리트의 복합열화에 관한 연구)

  • Kim Eun-Kyum;Choi Sang-Deok
    • Proceedings of the Korea Concrete Institute Conference
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    • 2005.05b
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    • pp.225-228
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    • 2005
  • This paper was accomplished for analyzing the reason of the above deterioration happened on the deck of concrete bridge. The bridge was constructed at 660m above the sea level having more freezing and snowing days. Therefore, it is placed on the particular condition sprinkling $CaCl_2$ enough for keeping up with moderate traffic condition. When it is considered to the former condition, the bridge can be assumed to potentialities for combined deterioration with freezing-thawing under sprinkling deicing chemical. Core specimens were gathered from the concrete deck for clearing the reason of the above deterioration exactly, and it is used for various tests for measuring the compressive strength, elastic modulus, content of $Cl^-$, freezing-thawing at the fresh and salt water. As a result of freezing-thawing test, the specimen at the fresh water has over 90$\%$ of durability factor, but another specimen at 1$\%$ of salt water has 0$\%$ of durability factor at 140 cycles of the freezing-thawing. The result means that frost damage is sccelerated at the salt water. Therefore, the deterioration of the concrete deck is estimated to be occured by combined effects of freezing-thawing and chloride ion attack.

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Properties of PD Pulses accompanying with propagation of Bush-type tree in LDPE (LDPE에서 부시형 전기트리의 성장에 수반되는 부분방전 펄스의 특성)

  • 박영국;강성화;정수현;박철현;임기조
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.06a
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    • pp.293-296
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    • 1998
  • Surface electrical conduction in insulator is most important factor to assess the insulation performances of outdoor insulating materials. In this paper, contamination performance of the widely used materials for outdoor insulator - porcelain, EPDM, Silicone rubber - were discussed by measuring properties of average leakage current and scintillation discharge pulses under artificial contamination conditions. The artificial contaminations used were deionized distilled water fog, 0.5wt% NaCl salt fog of light pollution and 2wt% NaCl salt fog of medium pollution. The average leakage current was appeared linearly with applied voltage at dry and clean surface condition. The magnitude of leakage current was almost same at different kinds of samples. In case of deionized distilled water fog, the characteristics of leakage current and applied voltage was most different to that in case of dry and clean condition. In case of salt fog pollution condition, The leakage current was increased above critical voltage. The scintillation discharges were also activated at the level. the leakage current and scintillation discharges were increased with increasing pollution degree. The resistance to pollution properties of silicone rubber appeared excellent among them.

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Properties of Surface Electrical Conduction in Materials for Outdoor Insulator (옥외 애자용 재료의 표면 전기전도특성)

  • 박영국;강성화;정수현;이운석;임기조
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 1998.06a
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    • pp.207-210
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    • 1998
  • Surface electrical conduction in insulator is most important factor to assess the insulation performances of outdoor insulating materials. In this paper, contamination performance of the widely used materials for outdoor insulator - porcelain, EPDM, Silicone rubber - were discussed by measuring properties of average leakage current and scintillation discharge pulses under artificial contamination conditions. The artificial contaminations used were deionized distilled water fog, 0.5wt% NaCl salt fog of light pollution and 2wt% NaCl salt fog of medium pollution. The average leakage current was appeared linearly with applied voltage at dry and clean surface condition. The magnitude of leakage current was almost same at different kinds of samples. In case of deionized distilled water fog, the characteristics of leakage current and applied voltage was most different to that in case of dry and clean condition. In case of salt fog pollution condition. The leakage current was increased above critical voltage. The scintillation discharges were also activated at the level the leakage current and scintillation discharges were increased with increasing pollution degree. The resistance to pollution properties of silicone rubber appeared excellent among them.

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Electrical Conduction Properties of Surface in Materials for Outdoor Insulator (옥외 애자용 재료의 표면 전기전도 특성)

  • 박영국;이운석;정수현;장동욱;임기조
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.11 no.10
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    • pp.758-762
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    • 1998
  • Electrical conduction property of insulator surface is most important factor to assess the insulation performances of outdoor insulating materials. In this paper, contamination performance of the materials to be used for outdoor insulator such as porcelain, EPDM, silicone rubber was discussed by measuring properties of average leakage current and scintillation discharge pulses under salt fog conditions. The fog was applied by nozzle in chamber and fogging fluids were deionized distilled water, 0.5wt% NaCI solution and 2wt% NaCl solution. The average leakage current showed linearly with applied voltage at dry and clean surface condition. The magnitude of leakage current was almost same at different kinds of samples. In case of deionized distilled water fog, the characteristics of leakage current and applied voltage were much different to those in case of dry and clean condition with 2wt% salt fog. In case of slat fog pollution condition, the leakage current was increased above critical voltage. the scintillation discharges were also activated at the level. The leakage current and scintillation discharges were increased with increasing pollution degree. The resistance to pollution properties of silicone rubber appeared excellent among them.

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Corrosion in Oil well Stimulation Processes Caused by Different Chelating Agents Based on EDTA Compounds

  • Calderon, J.A.;Vasquez, F.A.;Arbelaez, L.;Carreno, J.A.
    • Corrosion Science and Technology
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    • v.16 no.2
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    • pp.59-63
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    • 2017
  • Chelating solutions can be damaged by strong acids during oil production. To design effective corrosion inhibitors and other alternatives for corrosion control, it is important to understand not only the behavior of the system under operating condition but also the kinetics of electrochemical reactions during the corrosion process. In this study, the electrochemical behaviors of P-110 steel in aqueous fluids based on ethylenediaminetetraacetic acid (EDTA) compounds under various temperatures and hydrodynamic regime conditions were assessed. Electrochemical measurements were conducted using rotating disc electrodes manufactured. Electrolytes were prepared using aqueous compounds of EDTA like diammonium salt, disodium salt, and tetrasodium salt. Potentiodynamic polarization, electrochemical impedance, and mass loss tests were performed in order to assess the corrosion kinetic in electrolytes. Hydrodynamic effects were observed only in the cathodic polarization curve. This proves that hydrodynamic regime plays an important role in the corrosion of steel mainly in disodium and diammonium EDTA solutions. Two cathodic reactions controlled the corrosion process. However, oxygen level and pH of the electrolyte played the most important role in metal corrosion. Corrosion rates in those fluids were decreased drastically when oxygen concentration was reduced.

Development of TREND dynamics code for molten salt reactors

  • Yu, Wen;Ruan, Jian;He, Long;Kendrick, James;Zou, Yang;Xu, Hongjie
    • Nuclear Engineering and Technology
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    • v.53 no.2
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    • pp.455-465
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    • 2021
  • The Molten Salt Reactor (MSR), one of the six advanced reactor types of the 4th generation nuclear energy systems, has many impressive features including economic advantages, inherent safety and nuclear non-proliferation. This paper introduces a system analysis code named TREND, which is developed and used for the steady and transient simulation of MSRs. The TREND code calculates the distributions of pressure, velocity and temperature of single-phase flows by solving the conservation equations of mass, momentum and energy, along with a fluid state equation. Heat structures coupled with the fluid dynamics model is sufficient to meet the demands of modeling MSR system-level thermal-hydraulics. The core power is based on the point reactor neutron kinetics model calculated by the typical Runge-Kutta method. An incremental PID controller is inserted to adjust the operation behaviors. The verification and validation of the TREND code have been carried out in two aspects: detailed code-to-code comparison with established thermal-hydraulic system codes such as RELAP5, and validation with the experimental data from MSRE and the CIET facility (the University of California, Berkeley's Compact Integral Effects Test facility).The results indicate that TREND can be used in analyzing the transient behaviors of MSRs and will be improved by validating with more experimental results with the support of SINAP.

Early Growth, Carbohydrate and Phytic Acid Contents of Germinating Rice Seeds under NaCl Stress

  • Park So-Hyeon;Sung Jwa-Kyung;Lee Su-Yeon;Park Jae-Hong;Lee Ju-Young;Jang Byoung-Choon;Lee Ki-Sang;Song Beom-Heon;Kim Tae-Wan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.2
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    • pp.137-141
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    • 2006
  • Germination characteristics and alterations in soluble sugar-starch transition and phytic acid during germination were studied in rice seeds under saline conditions. NaCl significantly reduced the speed of germination. Also, the radicle growth out of seeds was severely inhibited by the exposure to NaCl solution, thus, seeds were almost impossible to grow to seedlings. Soluble sugar was remarkably accumulated, whereas starch was decomposed stepwise during seed germination. The metabolism of soluble sugar and starch in germinating seeds showed a distinct difference. The level of phytic acid in seeds decreased in all NaCl treatments during germination, but the level was affected differently by NaCl concentration in the two varieties. Overall, our results suggest that salt stress retard the radicle growth of rice seeds, and affect the starch-to-sugar conversion and the decomposition of phytic acid differently in two varieties.

Change in Photosynthesis, Proline Content, and Osmotic Potential of Corn Seedling under High-Saline Condition

  • Yoon Byeong Sung;Jin Chengwn;Park Sang Un;Cho Dong Ha
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.1
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    • pp.28-31
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    • 2005
  • To identify salt-tolerance characteristics of corn seedling was treated in solution of 0, 50 and 100 mM NaCl of hydroponic cultivation. In photosynthesis of corn seedling, there was no large difference between 50mM and 0 mM NaCl solution, however, in 100 mM NaCl solution, the tolerance gradually decreased to $76\%,\;49\%,\;and\;31\%$ after one day, four days, and seven days, respectively, in comparison to 0 mM NaCl solution. Osmotic potential of corn in seedling period was significantly decreased with increasing saline level, however, free proline content in the plant on the ground was significantly increased with increasing saline level and with the lapse of time. In terms of correlation among major characteristics, there was a highly significant positive difference between osmotic pressure potential and photosynthesis, However, highly negative correlation was found between osmotic pressure potential and free proline content. In addition, it was expected that young seedling of corn with saline tolerance may be utilized in the transplantation in salt-accumulated land. Based on above-shown result, in terms of saline tolerance of Chalok-2 variety, growth suppression was serious with 100mM NaCl solution. However, growth was expected that seedling growth would be favorable under 50 mM NaCl solution.