• Title/Summary/Keyword: rose bengal

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Formation of $I_2$ by a Photocatalytic Reaction of Rose Bengal (Rose Bengal의 광촉매 반응에 의한 $I_2$의 생성)

  • Yoon, Kil-Joong
    • Analytical Science and Technology
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    • v.9 no.1
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    • pp.72-77
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    • 1996
  • In the process of solar energy conversion into electrical energy using the photoelectrochemical cell containing the sensitizer, rose bengal and supersensitizer. $I^-$, the photocurrent is stabilized and durable. But the long time span of irradiation causes the decrease of photocurrent monotonically. Spectroscopic and electrochemical analyses of rose bengal solution containing $I^-$ revealed that the decrease of concentration of rose bengal was attributed to the reaction of rose bengal in the dark with $I_2$ formed as a result of the possible photocatalytic reaction of rose bengal with $I^-$.

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A Study on the Optimum Composition of Rose Bengal Reagent using Phase Transfer Catalyst (상이동 촉매 기법(phase transfer catalyst)을 이용한 rose bengal 시약의 최적 조성에 관한 연구)

  • Oh, Soo-Jin;Cha, Won-Jin;Choi, Da-Woon;Hong, Sung-Wook
    • The Journal of the Korea Contents Association
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    • v.18 no.7
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    • pp.245-252
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    • 2018
  • The rose bengal reagent, based on the phase transfer catalysis technique, is a calcium-targeting reagent that forms an insoluble salt that does not dissolve in a neutral or basic solution. It is expected to be effective in developing a latent fingerprint in a wet sample. However, many previous studies did not observe the fluorescence of the developed fingerprints, nor were the proposed methods of producing the reagents the optimal develop conditions. The aim of this study was to investigate the optimum reagent composition of rose bengal by varying the concentration of rose bengal dye and phase transfer catalyst based on maximum emission fluorescence of rose bengal. As a result, it was confirmed that rose bengal and surfactant concentration were the most effective when 0.01M: 0.008M, respectively.

Comparative Study of DNA Extraction Method in Meiofauna (중형저서동물에서 효율적인 DNA 추출 방법 비교 연구)

  • Lee, Seung-Han;Back, Jin-Wook;Lee, Won-Choel
    • Korean Journal of Environmental Biology
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    • v.29 no.3
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    • pp.138-143
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    • 2011
  • The efficiency of mtCOI amplication after DNA extraction of benthic harpacticoid Tigriopus japonicus s.l. was tested under different conditions depending on fixative (99% Ethanol, or 4% Formalin) and additional chemicals (Ludox or Rose Bengal). Each experimental group by the fixative was subdivided into four groups, respectively: 1) Control (fixative only), 2) processed with Ludox HS40, 3) processed with Rose Bengal, and 4) processed with both Ludox HS40 and Rose Bengal. For the 99% ethanol-fixed sample, overall success rate of amplification by PCR was 96% or above, while for the 4% formalin-fixed one, success rate was much lower than those of ethanol-fixed: 1) Control: 27%, 2) Ludox HS40: 3%, 3) Rose Bengal: 7%, and 4) Ludox HS40 and Rose Bengal: 3%. As a result present study verify that 99% ethanol is a proper fixative for DNA extraction in meiofauna organisms.

Study on iodine Labelling (II) Efficient of Labelling Rose Bengal, Hippuran, and Human Serum Albumin in Small Scale

  • Kim, Jaerok
    • Nuclear Engineering and Technology
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    • v.4 no.3
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    • pp.186-193
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    • 1972
  • For efficient micro scale syntheses of Rose $Bengal-^{131}I$, $Hippuran-^{131}I$, and $H.S.A.-^{131}I$, the dependence of labelling yields on pH, on salt contents, and on the volume of buffer solution in the reaction mixtures as well as the reaction apparatus were studied. pH of 5.6 was optimum for preparation of both Rose $Bengal-_{131}I$ and Hippuran $-^{13}I$ but pH of 8.5 was optimum for preparation of $H.S.A.-^{131}I$. Salt in the reaction mixtures hindered drastically the formation of $Hippuran-^{131}I$ but it slightly increased the labelling yield of H.S.A.. The compactly closed reaction vessels were effective for preparations of both Rose $Bengal-^{131}I$and $Hippuran-^{131}I$ in small volume. Thereupon, the labelling procedures were modified to bring about higher labelling yields and better reproducibilities. By these newly established procedures, the labelling yields of Rose $Bengal-^{131}I$ and $Hippuran-^{131}I$ could be increased even with the home-produced sodium $iodide-^{131}I$ solution containing reducing agent.

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Effect of Urea on the Rose Bengal Sensitized Photocurrent (Rose Bengal 감응 광전류에 미치는 Urea의 영향)

  • Yun, Gil Jung;Gang, Seong Cheol;Kim, Gang Jin
    • Journal of the Korean Chemical Society
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    • v.34 no.6
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    • pp.600-605
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    • 1990
  • Electron injection from excited rose bengal into the conduction band of a thin film of SnO$2$, semiconductor was investigated in an electrochemical cell, ITO/SnO$2$/rose bengal, NaClO$4$/Pt. It was observed that urea enhanced the supersensitized photocurrent, followed by the reduction in the photocurrent after a shallow maximum. Spectroscopic analyses of the dye solution containing urea revealed that dye aggregation appeared to be involved in the increased photocurrent, although the concentration of the dye was continuously decreased dye to photochemical reactions.

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Photocatalytic Reaction of Sensitizer, Rose Bengal and Supersensitizer, Thiourea (감응제 Rose Bengal과 초감응제 Thiourea의 광촉매 반응)

  • Yoon, Kil-Joong
    • Analytical Science and Technology
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    • v.9 no.1
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    • pp.62-71
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    • 1996
  • The enhancement of the solar energy conversion efficiency into the electrical energy by the dye sensitization with a photoelectrochemical cell was studied. The magnitude of the rose bengal sensitized photocurrent containing the supersensitizer, thiourea was five times greater than that in the absence of thiourea. It was observed, however, that the long time span of irradiation causes the decrease in the photocurrent. Spectroscopic analysis of the dye solution showed that the dye molecule was photobleached and the insoluble aggregate which settles down in the solution, was formed as a result of the possible photocatalytic reaction and the disappearance of dye from the solution was the cause of the decreased photocurrent in the sensitization run.

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A Study on the Photocatalytic Dimerization of Rose Bengal and Thiourea (Rose Bengal 과 Thiourea 의 광촉매 이합체화 반응에 관한 연구)

  • Yoon, Kil-Joong
    • Analytical Science and Technology
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    • v.12 no.3
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    • pp.209-217
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    • 1999
  • To elucidate the decay of the rose bengal (RB) sensitized and thiourea (TU) supersensitized photocurrent, spectroscopic analyses of the dye solution were performed. Absorption and fluorescence spectroscopic analyses of sensitizing solution before and after irradiation enabled to conform the new mechanism of the photocatalytic dimerization between RB and TU. And it was also found that the geometrical arrangement of the transition dipole moment is oblique and the angle between the dipoles is $124^{\circ}$ in the dimer of dye molecules.

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A Study on the Photocatalytic Dimerization of Rose Bengal and Allylthiourea (Rose Bengal과 Allylthiourea의 광촉매 이합체화 반응에 관한 연구)

  • Yoon, Kil-Joong;Hahm, Eun-Jeong;Kim, Kang-Jin
    • Analytical Science and Technology
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    • v.11 no.1
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    • pp.20-28
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    • 1998
  • Fluorimetric and absorption spectroscopic studies were performed to elucidate the photocurrent decay with time in the conversion process of solar energy into electrical energy using a photoelectrochemical cell containing rose bengal as a sensitizer, and allylthiourea as a supersensitizer. Spectra of dye solution before and after irradiation revealed a new photocatalytic dimerization reaction between sensitizer and supersensitizer. It was also found that the geometrical arrangement of the transition dipoles is oblique in the dimer of dye molecules.

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Diagnostic Significance of the Blood Disappearance Rate of $^{131}I$-Rose Bengal and of $^{198}Au$ colloid in Hepatobiliary Diseases (간담도질환(肝膽道疾患)에 있어서 $^{131}I$-Rose Bengal 및 $^{198}Au$ 교질(膠質)의 혈중소실율(血中消失率)의 진단적(診斷的) 의의(意義)에 관(關)한 연구(硏究))

  • Chun, Young-Kyoon;Koh, Chang-Soon;Hahn, Shim-Suk
    • The Korean Journal of Nuclear Medicine
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    • v.5 no.2
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    • pp.7-18
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    • 1971
  • The liver function test was performed by means of two radioisotope tracer techniques in 20 normal subjects and in 63 patients with hepatobiliary diseases. The blood disappearance rates of $^{131}I$-rose bengal and of $^{198}Au$ colloid were determined by external counting method. The hepatocellular function and the hepatic blood flow were estimated from the observed data and the results were compared with those of the conventional liver function tests. The results obtained were as follows: 1. The mean blood disappearance half time of $^{131}I$-rose bengal was $6.6{\pm}0.63$ minutes in normal control, $17.7{\pm}6.93$ in cirrhosis of the liver, $16.6{\pm}4.80$ in acute hepatitis, and $14.7{\pm}3.46$ in obstructive jaundice. It was markedly prolonged in the hepatobiliary diseases as compared with the normal control, but there was no significant difference among the hepatobiliary diseases. 2. The mean blood disappearance half time of $^{198}Au$ colloid was $4.0{\pm}0.66$ minutes in normal control, $9.8{\pm}3.42$ in cirrhosis of the liver, $4.4{\pm}0.82$ in acute hepatitis, and $5.0{\pm}1.42$ in obstructive jaundice. The difference between cirrhosis of the liver and normal control Was statistically significant. However, there was no definite difference among acute hepatitis, obstructive jaundice, and normal control. The mean blood disappearance rate constant (K value) was $0.177{\pm}0.028/minute$ in normal control. In cirrhosis of the liver, it was markedly decreased which was suggestive of the reduced hepatic blood flow. 3. The ratio of $^{131}I$-rose bengal blood disappearance half time to $^{198}Au$ colloid disappearance half time was $1.68{\pm}0.20$ in normal control, $1.82{\pm}0.31$ in cirrhosis of the liver, $3.80{\pm}0.82$ in acute hepatitis, and $3.01{\pm}0.54$ in obstructive jaundice. The ratios in acute hepatitis and obstructive jaundice were remarkably higher than those in normal control and cirrhosis of the liver. 4. There was a significant correlation between the blood disappearance half time of $^{131}I$-rose bengal and that of $^{198}Au$ colloid in cirrhosis of the liver. 5. In cirrhosis of the liver, the blood disappearance half times of $^{131}I$-rose bengal and of $^{198}Au$ colloid were inversely correlated to the serum albumin level. In acute hepatitis, there was a good positive correlation between the blood disappearance half time of $^{131}I$-rose bengal and the serum transaminase activities. In obstructive jaundice, the blood disappearance half time of $^{131}I$-rose bengal was correlated to the serum bilirubin level.

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The Effect of Ginseng Extracts on Photooxidation in Serum Lipid (혈청지질의 광산화 반응에 미치는 인삼추출물의 영향)

  • 백태홍;천현자
    • Journal of Ginseng Research
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    • v.13 no.2
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    • pp.234-238
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    • 1989
  • In the presence of a photosensitizer, rose bengal, phooxidatlon in serum total lipids has been studied and the effects of ginseng water extract and saponins on it have reviewed. In the presence of rose bengal, serum total lipids undergo photooxidation and produce lipid hydroperoxides. On the other hand, ginseng water extract and dial saponins largely inhibit photooxidation and decrease the amount of lipid hydroperoxides in serum total lipids.

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