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A Study on the Optimum Composition of Rose Bengal Reagent using Phase Transfer Catalyst

상이동 촉매 기법(phase transfer catalyst)을 이용한 rose bengal 시약의 최적 조성에 관한 연구

  • 오수진 (순천향대학교 법과학대학원) ;
  • 차원진 (순천향대학교 법과학대학원) ;
  • 최다운 (순천향대학교 법과학대학원) ;
  • 홍성욱 (순천향대학교 법과학대학원)
  • Received : 2018.06.08
  • Accepted : 2018.06.22
  • Published : 2018.07.28

Abstract

The rose bengal reagent, based on the phase transfer catalysis technique, is a calcium-targeting reagent that forms an insoluble salt that does not dissolve in a neutral or basic solution. It is expected to be effective in developing a latent fingerprint in a wet sample. However, many previous studies did not observe the fluorescence of the developed fingerprints, nor were the proposed methods of producing the reagents the optimal develop conditions. The aim of this study was to investigate the optimum reagent composition of rose bengal by varying the concentration of rose bengal dye and phase transfer catalyst based on maximum emission fluorescence of rose bengal. As a result, it was confirmed that rose bengal and surfactant concentration were the most effective when 0.01M: 0.008M, respectively.

상이동 촉매기법을 기반으로 한 rose bengal 시약은 중성이나 염기성 용액에서 녹지 않는 난용성 염을 형성하는 칼슘을 타겟팅하는 시약으로, 물에 젖은 검체에서 잠재지문 현출시 효과적일 것으로 기대되어 최근 연구가 이루어지고 있다. 그러나 그 동안 선행연구에서는 rose bengal 시약으로 현출된 지문의 형광 특성을 관찰하지 않았을 뿐만 아니라 제시된 시약의 제조법 또한 최적 현출 조건인지 검토되지 않았다. 이에 본 연구는 rose bengal 염료의 최대방출형광을 바탕으로 rose bengal염료와 상이동촉매제(Tetrabutylammonium)의 농도를 달리하여 최적의 시약 조성을 찾고자 하였다. 그 결과 rose bengal과 상이동촉매제의 농도가 각 0.01 M: 0.008 M일 때 가장 효과적인 것을 확인할 수 있었다.

Keywords

References

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