• 제목/요약/키워드: rootstocks

검색결과 95건 처리시간 0.03초

Greenhouse Evaluation of Melon Rootstock Resistance to Monosporascus Root Rot and Vine Decline as Well as of Yield and Fruit Quality in Grafted 'Inodorus' Melons

  • Jang, Yoonah;Huh, Yun-Chan;Park, Dong-Kum;Mun, Boheum;Lee, Sanggyu;Um, Yeongcheol
    • 원예과학기술지
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    • 제32권5호
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    • pp.614-622
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    • 2014
  • Melons (Cucumis melo L.) are generally grafted onto Cucurbita rootstocks to manage soilborne pathogens such as Monosporascus root rot and v ine decline (MRR/VD) and Fusarium wilt. However, g rafting onto Cucurbita rootstocks reportedly results in the reduction of fruit quality. In this study, the resistance to MRR/VD, yield, and fruit quality of melons grafted onto melon rootstocks were evaluated under greenhouse conditions. Eight melon rootstocks (R1 to R8) were used and the inodorus melon 'Homerunstar' was used as scion. Melon rootstocks R1 to R6 were selected based on resistance to MRR/VD under greenhouse conditions. Non-grafted 'Homerunstar' and plants grafted onto squash interspecific hybrid 'Shintozwa' rootstock (Cucurbita maxima D. ${\times}$ C. moschata D.) served as controls. Grafted melons were cultivated in the greenhouse infested with Monosporascus cannonballus during two growing seasons (summer and autumn). The responses to MRR/VD, yield, and fruit quality differed depending on the rootstocks and growing season. The melons grafted onto 'Shintozwa' exhibited less severe disease symptoms and higher survival rates than non-grafted melons in both seasons. While the melon rootstocks in the summer cultivation did not increase the survival rate compared to non-grafted melons, the melon rootstocks R1 and R2 in the autumn cultivation led to higher survival rates. The melon rootstocks resistant to MRR/VD increased the percentage of marketable fruits and marketable yields. Grafting onto the melon rootstocks caused little or no reduction of fruit quality such as low calcium content, fruit softening, and vitrescence, especially in lower-temperature autumn season. Accordingly, these results suggest that grafting onto the melon rootstocks may increase the tolerance to MRR/VD and the marketable yield without a reduction of fruit quality.

생장조절제 처리에 따른 과수화상벙 저항성 사과대목의 기내 식물체 유도 (Induction on in vitro Plant Regeneration the Apple Rootstocks of Fire Blight Resistance by Plant Growth Regulators)

  • 권영희;최원일;김희규;김경옥;김주형;송용섭
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2021년도 춘계학술대회
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    • pp.23-23
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    • 2021
  • Apple (Malus×domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. Tissue culture in vitro is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This could be important in the production of genetically uniform scions and rootstocks for commercial apple production. In nurseries, apple plants are produced by grafting scions onto rootstocks. The Cornell-Geneva (Geneva® series) breeding program has bred several dwarf rootstocks that are resistant to diseases and pests and are also cold hardy. This study was conducted to determine the optimal medium strength to improve sprouting shoot rate of apical meristem of the apple rootstocks of fire blight resistance. The apple rootstocks apical meristem at size (0.2 mm to 0.3 mm) with axillary buds were cultured on the MS(Murashige & Skoog) medium supplemented with plant growth regulators. The sprouting ratio and growth characteristics was evaluated after eight weeks in vitro culture. The highest rate of bud differentiation and shoot formation were 23.8% and 55.6%, respectively. After 6 weeks, shoots were regenerated from apical meristem, and their growth characteristics was significantly varied on the respective basal medium with different plant growth regulators. Our studies showed that the apple rootstocks the apple rootstocks of fire blight resistance plantlets could be successfully produced from apical meristem differentiated out of young twigs via organogenic regeneration.

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접목참외의 생육, 품질 및 양분흡수 특성에 관한 대목의 영향 (Effect of Rootstocks on the Growth, Fruit Quality, and Nurtient Contents in Various Parts of Oriental Melons (Cucumis mels L.var. makuwa MAKINO))

  • 정순재;구우서;정경태
    • 생명과학회지
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    • 제5권4호
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    • pp.190-202
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    • 1995
  • Two oriental melons cultivars, ‘Geumssaragi Euncheon’(GSEC) and ‘House Eunchon’(HEC), were grafted onto different rootstocks and the growth, mineral contents and fruit quality were examined. The seedlings grafted onto‘Geumtozwa’rootsticks showed about 10% defected seedling loss(wilting or dwarfing0as compared to the little or no loss in the seedlings grafted onto 'Sintozwa' or 'Chamtozwa' rootstocks. The seedings grafted onto 'Sintozwa' or 'Chamtozwa' rootstocks also exhibited better vine growth as compared to the intact seedlings or the seedlings grafted onto 'Geumtozwa' rootstocks. The fruits growth, as measured by fruits length, fruit diameter and fruit fresh weight, was accelerated by the rootstocks especially by 'Sintozwa' and 'Chamtozwa' . Intact oriental melons showed the highest soluble solids content in the fruit pericarp followed by 'Chamtozwa' rootstocks. Sucrose contents were higher in the seedling grafted onto 'Sintozwa', whereas higher glucose and fructose contents were measured in intact GSEC fruits or HEC fruits on 'Chamtozwa' rootstock. As compared to the intact plants, the seedling grafted onto 'Sintozwa' rootstock showed higher N. P and K and lower Ca and Mg contents in leaves, stems, roots and fruits. 'Geumtozwa' rootstock, developed specially for oriental melons, showed very similar pattern of mineral absorption or distribution as compared to the intact plants.

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Growth and Development of Grafted Cucumber Transplants as Affected by Seedling Ages of Scions and Rootstocks and Light Intensity during Their Cultivation in a Closed Production System

  • Kwack, Yurina;Park, Seon Woo;Chun, Changhoo
    • 원예과학기술지
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    • 제32권5호
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    • pp.600-606
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    • 2014
  • The objective of this study was to determine the effects of seedling ages of scions and rootstocks for grafting and light intensity during their cultivation in a closed transplant production system on the growth and development of grafted cucumber transplants. Cucumber scions and rootstocks were cultivated under 5 photosynthetic photon flux (PPF) levels: 100, 140, 180, 230, and $280{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ in a closed transplant production system. The scions were grafted onto the rootstocks at 8, 9, 10, 11, and 12 days after sowing (DAS). Hypocotyl length of scions and rootstocks decreased significantly as PPF increased, and an increase in dry weight with increasing PPF was more pronounced in scions. After grafting, cucumber transplants were grown in a greenhouse until 22 DAS and were then transplanted for investigation of their growth and development. Plant height, leaf area, and fresh weight of cucumber transplants grafted at 8, 9, and 10 DAS were greater, but light intensity during cultivation of scions and rootstocks did not significantly affect the early growth of cucumber transplants after grafting. The number of female flowers in grafted cucumber after transplanting was highest when scions and rootstocks were cultivated under PPF 140 and $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and were grafted at 8 DAS. These results indicate that controlling environmental conditions in a closed transplant production system during the production of scions and rootstocks can advance grafting time and promote the growth and development of grafted cucumber transplants.

Evaluating the resistance to crown gall in grape rootstocks.

  • Park, Jeong-Ho;Yun, Hae-Keun;Park, Kyo-Sun;Lee, Chang-Hoo;Jeong, Sang-Bouk
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.70.2-70
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    • 2003
  • To evaluate the resistance to crown gall in grape rootstocks, cuttings from twenty seven grape rootstocks were inoculated with Agrobacterium vitis Cheonan 493 and size of galls from grapevines was measured in a greenhouse. Tumors were formed in all varieties of grape rootstocks tested in this study and no grape rootstock variety was immune to crown gall. Tumors were found on the stems of all plants tested in '196-17'and '41B' Based on measuring size and weight of galls formedon the stem of grape rootstocks, '779P' was extremely susceptible to crown gall. Some varieties such as 'Gloire', '140R', '101-l4M', '3309C', and '333EM' found to be resistant, while '99R', '1447P', 'Rupestris du lot', '110R', 'Freedom', and '41B'were susceptible and '1103P', '5C', '420A', 'Golia', and '5BB' were moderately susceptible to crown gall.

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박과채소의 대목에 따른 복부분비액, 무기성분 및 식물호르몬의 함량 (Effect of Cucurbitaceous Crops and Rootstocks on the Xylem Sap, Amount Mineral Element and Plant Hormone)

  • 정순재
    • 생명과학회지
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    • 제8권1호
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    • pp.97-101
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    • 1998
  • 박과채소류의 접목에 사용되는 대목의 종류 및 접목오이와 참외의 목부분비액의 양, 무기성분 및 식물호르몬 함량에 관하여 실험을 실시하였던 바 그 주요결과는 다음과 같이 요약된다. 100g 식물 생체중당 m1로 표현괸 목부분비량은 '흑종'대목이 다른 매복에 비하여 놓았으며 분비액내의 선기 전도도와 산도는 대복에 따라 큰 영향을 받지 않았다. 접목오이의 목부분비액의 양은 '흑종'호박 접목묘가 자근묘나 '키토라'접목보다 높았고, 무기성분 함량에 있어서는 전질고, 인산 및 K은 접목에 의해 증가되었고 Ca와 Mg은 자근묘보다 감소하였다. 접목참외에 있어서는 목부분비액의 양은 '금보라'참외에서는 적토좌 접목묘에서, '신은천'참외에서는 '신토좌'점목묘에서 높았다. 무기성분함량은 '신은천'참외에서는 전질소는 대 간에 차이가 없고 K와 Mg은 접목에 의해 증가되었다. '금보라'참외에서는 전실소, Ca 및 Mg은 증가하였고 K은 감소하였다. 점복참외의 목부분비액내 cytokinin 유사물질의 함량은 자근묘와 '신토좌'접목묘에서 높았다.에서 높았다.

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감귤 대목과 그것에 접목한 온주밀감의 엽록소 형광특성의 비교 (Comparison of Chlorophyll Fluorescence of Three Citrus Rootstocks and Satsuma Mandarin Grafted on Them)

  • 한상헌
    • 원예과학기술지
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    • 제19권2호
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    • pp.149-152
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    • 2001
  • 3종의 감귤대목 탱자, 비룡, 시트르 메로와 그것에 접목한 온주밀감 '일남 1호'의 $CO_2$ 동화를 평가할 수 있는 엽록소 형광을 비교하였다. 강세 대목인 시트르 메로와 중간 대목인 탱자는 잠재적이며 실재적으로 거의 같은 광양자 흡수율을 나타냈지만, 약세 대목인 비룡은 암조건과 명조건에서 얻어진 Fo, Fs가 높기 때문에 상대적으로 낮은 광양자 흡수율을 보였다. 이러한 결과는 비룡은 흡수한 광 에너지 안테나 엽록소로부터 형광으로 손실이 되는 율이 높다는 것을 시사하고 있다. 그러나 이들 대목에 접목을 한 온주밀감은 잠재적이며 실재적으로 거의 같은 광양자 흡수율을 나타냈다. 이러한 사실로부터 대목은 접수의 양자 흡수율에 직접 영향을 미치지 않는다고 사료된다.

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고랭지 착색단고추의 접목재배 효과 (Effects of Grafting Cultivation on the Growth and Yield of Paprika in Highlands)

  • 이종남;이응호;김점순;김원배;류승열;용영록
    • 생물환경조절학회지
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    • 제13권1호
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    • pp.33-38
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    • 2004
  • 본 시험은 고랭지에서 착색단고추의 접목재배로 토경재배시 지하부 환경개선을 통해 토양전염성 병을 예방하고, 수량성을 향상시키고자 저항성 대목들을 수집하여 접목재배하여 생육, 수량 및 병저항성을 비교하였다. 접수는 적색계인 스피리트품종을 사용하였다. 역병균을 접종한 결과 자근묘인 Spirit는 접종 후 5일 만에 100% 고사하였고, 저항성이 높은 대목으로는 탄탄대목, Skurt-S, AC 2258, PST 8301 등 4품종이었다. 또한 청고병에 저항성이 높은 대목으로는 역강, 탄탄대목, TE 412, 갤럭시, MC 4, PST VK, PST NV 등 7품종이었다. 접목구는 지근묘에 비해 근중이 무거워 지하부의 환경개선효과가 뚜렷하였다. 접목과 비접목에 따른 과실의 외적 ${\cdot}$ 내적인 품질은 큰 차이가 없었다. 수량성이 높은 대목은 PST 8301, MC 4, 왕건 등이었다.

사과 왜성대목들의 줄기 황화처리에 따른 발근 부위의 생리적 변화 (Physiological Changes in Rooting Zone of Dwarf Apple Rootstocks (Malus domestica Borkh.) after Stem Etiolation Treatment)

  • 권순일;김목종;백봉렬;남종철;강인규
    • 생물환경조절학회지
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    • 제16권2호
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    • pp.151-156
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    • 2007
  • 본 시험은 주요 왜성대목인 M.26 과 M.9, 발근력이 낮은 O.3, 발근력이 높은 Mo.84 등 4 종류의 사과 대목 품종을 재료로 휘묻이 번식시 황화처리에 따른 발근 부위의 생리적 변화를 시기별로 각각 비교, 분석하여 부정근 발생에 관여하는 요인을 구명하고자 하였다. 휘묻이 번식시 대목별 발근율은 Mo.84가 가장 높았고, O.3가 가장 낮았다. 대목 별 휘묻이 전 후의 IAA함량은 황화 처리 전 발근력이 높은 Mo.84에서 가장 높았으나 휘묻이 일수가 경과할 수록 IAA 힘량 변화는 일정한 경향이 없었다. 반면 발근력이 기장 좋은 Mo.84의 ABA 함량은 휘묻이 처리 전 후 모두 가장 낮게 나타났다. 또한 휘묻이 처리전에 비하여 처리후에서 ABA 함량은 모든 대목 품종에서 감소하였다. Boron의 함량은 발근율이 낮은 O.3에서 가장 낮게 나타났으며, C/N율은 모든 대목들에서 발근율이 높은 Mo.84 대목이 가장 높게 나타났다. 그러므로, 대목의 휘묻이 처리에 따른 IAA 함량은 대목의 발근율에 미치는 주요한 요인이 아닌 것으로 판단되지만 ABA 함량과 boron의 수준은 사과왜성대 목들의 발근을 제한하는 요인으로 추정된다.

CGMMV-CP 형질전환 수박대목의 CGMMV 내성시험 및 계통확보 (CGMMV Tolerance Test of CGMMV-CP Trangenic Watermelon Rootstock and Establishment of Transgenic Line)

  • 박상미;권정희;임미영;신윤섭;허남한;이장하;류기현;한지학
    • Journal of Plant Biotechnology
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    • 제34권1호
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    • pp.11-17
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    • 2007
  • 수박작물의 대목용으로 사용하는 수박공대에 CGiMMV-CP 유전자를 도입하여 개발된 LM 수박공대의 CGMMV 내성 정도를 격리하우스와 노지 포장내에서 조사하였다. 격리온실에서의 $T_{3}$ 형질전환 수박대목의 CGMMV 내성은 접종 후 70일까지 유지되는 반면 대조구는 접종 후 20일에 전부 이병되었다. 인위적 토양전염 포장에서 형질전환체는 접종후71일까지 약 40%의 내성률을 보였으며, 대조구는 접종 후 37일에 모두 이병되었다. 인위적 접촉전염 포장에서 형질전환체는 대조구에 비해 약 10일 정도 지연효과를 보였다. 따라서 CGMMV-CP 형질전환체는 CGMMV에 저항성을 가진 것이 아니라 감염시기를 지연시키는 부분 내성으로 나타났다. CGMMV-CP homozygous T 세대를 진전시켜서 형질전환 수박공대 계통을 $BC_{1}T_{5}$ 세대에서 선발하였다. 또한 LM 수박공대에 형질전환 되지 않은 접수 (슈퍼금천수박)를 접목하여 non-LM 수박을 생산하고 CGMMV-CP 유전자에 관련된 물질의 이동 여부를 조사하였다. PCR, northern, western 분석한 결과 수박공대 대목에서 형성되는 DNA, RNA, protein 물질이 접수로 이동되지 않음을 확인하였다.