과제정보
본 연구는 농촌진흥청 공동연구사업 "과수화상병 등 현안 문제병해충 피해경감기술 개발/과수화상병 등 문제병해충 특성조사 및 방제법개발(과제번호: PJ0155492021)의 지원을 받아 수행되었습니다.
한국자원식물학회:학술대회논문집 (Proceedings of the Plant Resources Society of Korea Conference)
- 한국자원식물학회 2021년도 춘계학술대회
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- Pages.23-23
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- 2021
생장조절제 처리에 따른 과수화상벙 저항성 사과대목의 기내 식물체 유도
Induction on in vitro Plant Regeneration the Apple Rootstocks of Fire Blight Resistance by Plant Growth Regulators
- 권영희 (충청북도농업기술원 원예연구과) ;
- 최원일 (충청북도농업기술원 원예연구과) ;
- 김희규 (충청북도농업기술원 원예연구과) ;
- 김경옥 (충청북도농업기술원 원예연구과) ;
- 김주형 (충청북도농업기술원 원예연구과) ;
- 송용섭 (충청북도농업기술원 원예연구과)
- Young Hee Kwon (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
- Won IL Choi (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
- Hee Kyu Kim (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
- Kyung Ok Kim (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
- Ju Hyoung Kim (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services) ;
- Yong Sup Song (Horticultural Research Division, Chungcheongbuk-do Agricultural Research and Extension Services)
- 발행 : 2021.04.29
초록
Apple (Malus×domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. Tissue culture in vitro is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This could be important in the production of genetically uniform scions and rootstocks for commercial apple production. In nurseries, apple plants are produced by grafting scions onto rootstocks. The Cornell-Geneva (Geneva® series) breeding program has bred several dwarf rootstocks that are resistant to diseases and pests and are also cold hardy. This study was conducted to determine the optimal medium strength to improve sprouting shoot rate of apical meristem of the apple rootstocks of fire blight resistance. The apple rootstocks apical meristem at size (0.2 mm to 0.3 mm) with axillary buds were cultured on the MS(Murashige & Skoog) medium supplemented with plant growth regulators. The sprouting ratio and growth characteristics was evaluated after eight weeks in vitro culture. The highest rate of bud differentiation and shoot formation were 23.8% and 55.6%, respectively. After 6 weeks, shoots were regenerated from apical meristem, and their growth characteristics was significantly varied on the respective basal medium with different plant growth regulators. Our studies showed that the apple rootstocks the apple rootstocks of fire blight resistance plantlets could be successfully produced from apical meristem differentiated out of young twigs via organogenic regeneration.